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Citations for Cell Biolabs :
1 - 44 of 44 citations for Mouse ADAMTS16 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... the U6-VDAC1-shRNA sequences were then cut using ApaI and BstEI to be cloned into a pAAV-CMV-GFP vector (Cell Biolabs, Inc.), the pAAV-mito-GCaMP2 ...
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bioRxiv - Biochemistry 2019Quote: ... 10‐20 μg plasmid DNA and 5 μg helper plasmid (pCMV-Gag-Pol, Cell Biolabs) were used ...
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bioRxiv - Immunology 2021Quote: ... a pAAV6-Rep-Cap plasmid (both Cell Biolabs), and an ITR-flanked transfer plasmid at a 1:1:1 molar ratio ...
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bioRxiv - Biochemistry 2023Quote: ... were seeded into 10 cm2 plates and transfected at 90% confluency with 20 µg plasmid DNA and 10 µg helper plasmid (pCMV-Gag-Pol, Cell Biolabs) using CalPhos Mammalian Transfection Kit (Clontech®/Takara ...
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bioRxiv - Biochemistry 2019Quote: ... and the sense guides (sgRNA1) into the puromycin-selectable pBABED P U6 plasmid (Dundee-modified version of the original Cell Biolabs pBABE plasmid, DU48788). Cells at about 80% confluency were co-transfected in 10-cm dishes with 2 µg each of the sgRNA plasmid pairs and 20 µl of PEI stock solution (1 mg/ml) ...
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bioRxiv - Neuroscience 2019Quote: ... trans plasmids pAAV2/1 (Cell Biolabs Inc., Cat# VPK-421), pAAV2/2 (Cell Biolabs Inc. ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal Rac1 (240106; Cell Biolabs) at 1:100 ...
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bioRxiv - Immunology 2021Quote: ... Plasmids pAAV-GFP and pAAV-mCherry were purchased from Cell Biolabs. All the constructed plasmids were sequenced to confirm correct fragment insertion (Retrogen ...
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bioRxiv - Neuroscience 2023Quote: ... and RapCap DJ plasmids (Cell Biolabs Inc., San Diego, CA, USA). From 48-72 hours after transfection ...
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bioRxiv - Neuroscience 2024Quote: ... and RapCap DJ plasmids (Cell Biolabs Inc., San Diego, CA, USA). From 48-72 hours after transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies (mouse anti-yH2AX, Cell Biolabs, STA-321 ...
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bioRxiv - Synthetic Biology 2019Quote: The AAV6 capsid plasmid pRC6 was purchased from Cell BioLabs (San Diego, CA). AAV9 capsid plasmid p5E18-VD2/9 was obtained from the University of Pennsylvania Vector Core ...
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bioRxiv - Immunology 2021Quote: ... pHelper (helper plasmid) and pAAV-DJ (catalog number VPK-430-DJ, Cell Biolabs), as described [13] ...
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bioRxiv - Physiology 2022Quote: ... The scAAV-CMV-GFP plasmid was purchased from Cell Biolabs (Cat. No. AAV410). The insulin-like growth factor 1 (IGF1 ...
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bioRxiv - Cell Biology 2024Quote: ... The mouse fibroblast cell line SNL (Cell Biolabs) was used as feeder cells during the reprogramming process of infected NSCs ...
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bioRxiv - Neuroscience 2020Quote: ... was assembled and transfected with helper-free DJ plasmids (Cell Biolabs, San Diego, USA). Viral particles were purified by HiTrap heparin column (GE Healthcare) ...
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bioRxiv - Cancer Biology 2022Quote: ... along with the envelope plasmid pCMV-VSV-G (Cell Biolabs, San Diego, CA, USA), into cells Plat-GP packaging cells (Cell Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... at 70% confluency were transfected with a mix of Eco envelope plasmid (Cell Biolabs, 320026), pCMVR8.74 (Addgene ...
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Mammalian-unique eIF4E2 maintains GSK3β proline kinase activity to resist senescence against hypoxiabioRxiv - Cell Biology 2020Quote: Adeno-associated viruses were generated using packaging plasmids AAV-helper and AAV-8 (Cell Biolabs, Inc) together with pAAV-TOPBP1 ...
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bioRxiv - Genetics 2021Quote: ... barcoded sgRNA plasmid library carrying a neomycin-resistance cassette was packaged in PlatinumE cells (Cell Biolabs) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... two micrograms of retroviral plasmid DNA was transfected into platinum-E cells (Cell Biolabs; RV-101) using the FuGENE HD transfection reagent ...
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bioRxiv - Immunology 2022Quote: ... mouse anti-GAPDH (1:1,000; Cat#: AKR-001, Cell Biolabs Inc.), rabbit-anti Influenza A H1N1 HA (1:1,000 ...
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bioRxiv - Neuroscience 2021Quote: ... using the AAV-DJ/8 Helper Free Packaging System containing pAAV-DJ/8 and pHelper plasmids (Cell Biolabs). The triple plasmids (AAV-DJ/8-NPGL-IRES-GFP or AAV-DJ/8-IRES-GFP ...
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bioRxiv - Neuroscience 2020Quote: ... The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). The PHP.eB capsid variant was generated by Dr ...
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bioRxiv - Microbiology 2021Quote: ... grown in 10 cm dish were transiently transfected with the following plasmids: 5 µg of pLenti-GFP (Cell Biolabs), 6 µg of psPAX2 and 0.9 µg of pCMV-VSVG (Cell Biolabs) ...
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bioRxiv - Neuroscience 2022Quote: ... The AAV293 packaging cell line and plasmid supplying the helper function are available from a commercial source (Cell Biolabs). The PHP.eB capsid variant was generated by Dr ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: HDL was isolated from mouse plasma using HDL Purification Kit (Cell BioLabs, Inc.) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... or a LAT-eGFP P2A NFAT-mCherry plasmid-containing supertatants collected from Platinum-Eco cells (Cell Biolabs, San Diego, CA). T cells were imaged on days 5-8 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells (ATCC, CRL-3216) were transfected with each construct and third generation lentiviral packaging plasmids (Cell BioLabs, VPK-206) using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: The HA-tagged mouse Bhlhe40 coding sequence was excised from a pCAGGS-DEC1 plasmid (RDB08473, Riken, Saitama, Japan; ref. 52) and subcloned into pcDNA3.1 and pMXs-Puro Retroviral vector (RTV-012, Cell Biolabs, Inc.). For knockdown experiments ...
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bioRxiv - Neuroscience 2022Quote: ... HEK293T cells were transiently transfected with the ITR-flanked transgene together with three separate packaging plasmids including pHelper Vector (340202, Cell Biolabs), pAAV-RC1 Vector (VPK-421 ...
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bioRxiv - Immunology 2023Quote: Homology donors comprising sequences to be inserted and flanking homology arms were cloned into plasmid ITR-CMV-GFP (containing ITRs from AAV2; Cell Biolabs) or pVAX by Infusion cloning (Takara) ...
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bioRxiv - Cell Biology 2022Quote: ... For production of virus for mouse cells platinum E cells (Cell Biolabs, Cat#RV-101) were transfected ...
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bioRxiv - Biochemistry 2021Quote: ... The plasmid expressing the VSV-G (vesicular stomatitis virus glycoprotein) was purchased from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). The lentiviral plasmid used to generate the HEK-293T stable cell lines expressing the human ACE2 gene (GenBank ID NM_021804 ...
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bioRxiv - Biochemistry 2021Quote: ... while the plasmid expressing VSV G (vesicular stomatitis virus glycoprotein) was purchased from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). The HDM-IDTSpike-fixK plasmid was employed as a template for site-directed mutagenesis to generate the expression plasmid for the D614G mutant of SARS-CoV-2 spike protein ...
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bioRxiv - Neuroscience 2019Quote: ... HEK293T cells were transfected with the pAAV-hSyn-OsTIR1-P2A-mAID-EGFP and two AAV helper plasmids encoding serotype DJ (Cell Biolabs, San Diego, CA, USA) using polyethylenimine (Polysciences ...
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bioRxiv - Neuroscience 2019Quote: ... was introduced via AAV-mediated expression under the synapsin promoter (addgene plasmid #51085 deposited by Jonathan Ting was packaged in-house with the AAV-DJ Helper Free Packaging System of Cell Biolabs; 0.01 µl crude lysate/well). Overexpression of hTau.P301L was induced via AAV-mediated transduction of an expression vector under the synapsin promotor [70,71] and were administered at 3 DIV at MOI 300.
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bioRxiv - Physiology 2023Quote: ... The AAV-HSA-GFP plasmid was developed by inserting a human HSA promoter and GFP (ZsGreen1) into the promoterless AAV vector (Cell BioLabs, Cat. No. VPK-411-DJ) using In-Fusion Cloning (Takara Bio ...
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bioRxiv - Biochemistry 2022Quote: ... The mouse Mafa coding sequence was amplified by PCR and subcloned into pAAV-MCS (VPK-410, Cell Biolabs. Inc.).
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bioRxiv - Genetics 2020Quote: ... and glucose (#STA-681) in mouse plasma were determined using the appropriate kit (all purchased from Cell Biolabs, Inc). Hearts and whole aortas (from the aortic arch to the iliac artery ...
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bioRxiv - Biochemistry 2022Quote: ... The FLAG-tagged mouse Pdx1 coding sequence was amplified by PCR and subcloned into a pMXs-Neo Retroviral vector (RTV-011, Cell Biolabs, Inc.). pGL3-basic-MafA plasmid (−10427/+22 from transcription start site ...
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bioRxiv - Neuroscience 2021Quote: ... The full-length open reading frame of mouse Npgl was amplified from cDNA of the mediobasal hypothalamus and inserted into the pAAV-IRES-GFP expression vector (Cell Biolabs, San Diego, CA). The primers for mouse NPGL were 5’-CGATCGATACCATGGCTGATCCTGGGC-3’ for the sense primer and 5’-CGGAATTCTTATTTTCTCTTTACTTCCAGC-3’ for the antisense primer.
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bioRxiv - Neuroscience 2020Quote: ... targeting the open reading frame of mouse Tenascin-R into adeno-associated viral (AAV) vector U6 GFP (Cell Biolabs Inc., San Diego, CA 92126, USA), using BamH1 and EcoR1 restriction sites (Okuda et al ...
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bioRxiv - Plant Biology 2022Quote: ... Ten micrograms of total protein were separated on 10% SDS- PAGE gel and were followed by protein blot analysis using mouse anti- methylglyoxal monoclonal antibodies (Cell Biolabs, Catalog number- STA-011).