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Citations for Lucigen :
1 - 45 of 45 citations for Human Apolipoprotein AI B Free Serum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... PCR-free library preparation (Lucigen) and NextSeq 500 sequencing (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: ... (ii) Shotgun PCR-free library preparation kit (Lucigen) or (iii ...
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bioRxiv - Microbiology 2023Quote: ... Free nucleic acids were digested using OmniCleave Endonuclease (Lucigen, USA) for 30 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were lysed with 4000U of RNase-free lysozyme (Lucigen) at 37 °C for 2 hours ...
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bioRxiv - Plant Biology 2019Quote: ... the total RNA was treated with RNase free DNase1 (Epicentre Biotechnologies, USA) by incubating at 37 °C for 15 min ...
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bioRxiv - Molecular Biology 2019Quote: ... rRNA was depleted using the Ribo-Zero kit Human/Mouse/Rat (Epicentre), and libraries were prepared using random priming ...
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bioRxiv - Molecular Biology 2020Quote: ... with ribosomal depletion using Human/Mouse/Rat Ribo-Zero kit (Epicentre/Illumina). All samples were sequenced using a 100 base-pair (bp ...
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bioRxiv - Genomics 2022Quote: ... and patients A and B healthy and cancer tissue RNAs (500 ng) were incubated with 20 U exonuclease I (Lucigen) and 2 U Baseline-ZERO DNase (Lucigen ...
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bioRxiv - Genomics 2019Quote: ... Gram-negative and Human/mouse/rat Ribo-Zero rRNA Removal Kit (Epicentre Technologies). The resulting RNA was purified using Zymo RNA clean & Contentrator-5 column kit (ZYMO Research) ...
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bioRxiv - Molecular Biology 2019Quote: Five micrograms of small RNAs was dissolved in nuclease-free water and incubated with TEX (Lucigen) for 1 h at 30°C according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: The DNA libraries for Illumina sequencing were prepared using a Shotgun PCR-free library preparation (Lucigen) Illumina Library at the McGill University and Genome Quebec Innovation Center (Montréal ...
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bioRxiv - Microbiology 2020Quote: ... Free mRNA and rRNA ends were then ligated into circular forms with a circRNA ligase (Lucigen); it favors ligation events between proximal RNA ends11 and it has limited activity at high temperature ...
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bioRxiv - Physiology 2022Quote: ... we added 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen, Middleton, USA) and incubated for 15 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then blocked in a probe-free hybridization reaction mixture of 1XAmplifase buffer (Lucigen, A1905B), 0.05M KCl ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were then blocked in a probe-free hybridization reaction mixture of 1XAmplifase buffer (Lucigen, A1905B), 0.05M KCl ...
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bioRxiv - Synthetic Biology 2022Quote: ... two ncRNA expression cassettes (for barcoded ncRNAs “A” and “B”) from the Marionette plasmids were cloned into pSol-TSF (Lucigen F843213-1) facing in opposite directions ...
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bioRxiv - Genomics 2019Quote: ... The sample was placed on ice and preincubated with the CircLigase II reaction buffer on ice for 5 min (Nuclease-free H2O, 1 × CircLigase buffer, 2.5 mM MnCl2, 0.5 M Betaine, [all included in kit from CL9025K, Epicentre]). After aspiration the CircLigase II reaction mix (same buffer composition as previous but with 1U/µl of CircLigase II enzyme ...
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bioRxiv - Neuroscience 2021Quote: ... we added a 50 µL DNase mix containing 30 U RNase-free DNase I (Lucigen Corporation, Middleton, USA) together with the appropriate buffer and incubated for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... For RNAse-free IP conditions TBS-0.05% NP-40 was supplemented with 1U/ul NxGen RNAse inhibitor (Lucigen). For nuclease treated samples ...
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bioRxiv - Genomics 2020Quote: ... rRNA free samples were used for RNA-seq library preparation using ScriptSeq v2 RNA-Seq Library Preparation Kit (EPicentre). Microsome-polysomal RNA was obtained using a previously described method with some modifications (Li et al. ...
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bioRxiv - Plant Biology 2021Quote: ... a PCR-free library was prepared with the NxSeq® AmpFREE Low DNA Library Kit (Lucigen, Middleton, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Purified genomic DNA from each individual was used to prepare paired-end libraries (Lucigen Shotgun library preparation, PCR free) and sequenced on an Illumina HiSeq X PE 150 bp platform to 10-20X coverage at the Centre d’ Expertise et de Services ...
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bioRxiv - Biochemistry 2019Quote: ... The human gDNA was isolated from U2OS cells using the QuickExtract™ DNA Extraction Solution (EPICENTRE). From the gDNA ...
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bioRxiv - Biochemistry 2023Quote: ... Extraction of human genomic DNA was carried out using the QuickExtract DNA Extraction Solution (Lucigen, QE09050) following the standard protocol ...
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bioRxiv - Microbiology 2022Quote: ... DNA-Seq library preparation and sequencing were performed by the Genome Quebec Company (Genome Quebec Innovation Center, McGill University, Montreal, Canada) using the Shotgun PCR-free library preparation kit (Lucigen) and the NovaSeq™ 6000 Sequencing system (Illumina® ...
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bioRxiv - Neuroscience 2019Quote: ... After the second round of amplification contaminant cDNA was digested by treating the samples with RNAse-free DNAse (2U, Epicentre). Performance of the samples was tested by RT-PCR ...
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bioRxiv - Genomics 2022Quote: ... and FACS-sorted into 96-well PCR plates containing 3μl mild lysis buffer (nuclease-free water with 0.2% Triton + 0.1U/μl RNase inhibitor (Lucigen, Cat. 30281-2)) ...
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bioRxiv - Neuroscience 2022Quote: ... After the second round of amplification contaminant cDNA was digested by treating the samples with RNAse-free DNAse (2U, Epicentre). Performance of the samples was tested by RT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... and purified DNA-free RNA samples were subjected to ribosomal depletion with Ribo-Zero™ Magnetic Kits (Epicentre®, Singapore), all according to manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2020Quote: Only cytoplasmic RNA was treated with the Ribo-Zero Magnetic Gold Kit for human/mouse/Rat (Epicentre) to remove ribosomal RNA ...
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bioRxiv - Neuroscience 2023Quote: Genomic DNA from human iPSC-RPE was isolated by adding 40 µL QuickExtract DNA Extraction Solution (Lucigen) to each well ...
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bioRxiv - Immunology 2021Quote: ... the GeCKOv2 plasmid library with 6 guide RNAs per human gene was propagated in Endura ElectroCompetent cells (Lucigen), and lentivirus stocks were generated by co-transfecting 293T cells with the GeCKOv2 plasmid library ...
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bioRxiv - Molecular Biology 2019Quote: ... 5µg of total RNA was subjected to rRNA depletion using the RiboZero Human/Mouse/Rat kit (Epicentre Biotechnologies). cDNA was generated from the depleted RNA using random hexamers or custom primers and Superscript III (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA was depleted of ribosomal RNA using Ribo-Zero rRNA Removal Kit (Epicentre/Illumina, Human/Mouse/Rat) and library preparation was completed using NEBNext® Ultra Directional RNA Library Prep Kit for Illumina® (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... Purified RNA was subjected to ribosomal RNA depletion using the Ribo-Zero Magnetic Gold Kit (Human/Mouse/Rat; Epicentre), according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... rRNA was depleted using Ribo-Zero™ Magnetic Gold Kit for rRNA depletion (Human/Mouse/Rat) (Epicentre for Illumina #MRZG12324) according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA (rRNA) was depleted from RNA used for transcriptome analysis using Ribo-Zero rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... about 15 μg of total RNA of human frontal cortex were treated with 10 units of RNase R (RNR07250, Epicentre) for 1 hour at 37 °C and purified by phenolchloroform extraction ...
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bioRxiv - Molecular Biology 2022Quote: ... were sent to BGI (Hongkong) for ribosomal RNA depletion using Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) (Epicentre) followed by library construction using non-stranded (Replicate 1 ...
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bioRxiv - Immunology 2019Quote: ... The DNase-treated RNA (2 μg) was treated with Ribo-Zero using an Epicentre Ribo-Zero Gold Kit (Human/Rat/Mouse) (Epicentre) and re-purified on Ampure XP beads.
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bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from collected tissues and primary cultures was isolated using a standard TRIzol protocol followed by ribodepletion with a Ribo-Zero Gold rRNA Removal Kit (Human/Mouse/Rat) according to the manufacturer’s instructions (Epicentre; RZG1224). Strand-specific libraries were prepared using a TruSeq RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Systems Biology 2020Quote: ... Purified RNA (2.5 μg) was used for ribosome depletion using a Ribo-Zero™ Gold Kit (Human/Mouse/Rat) (Epicentre Biotechnologies) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... 5μg of RNA was subjected to ribosomal and mitochondrial RNA depletion using the RiboZero Gold kit (Human/Mouse/Rat, Epicentre #MRZG12324) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: One microgram of total RNA was rRNA depleted using the Ribo-Zero rRNA Removal Kit (Human, Mouse, Rat) (Epicentre, Madison, WI, USA) followed by a purification step using AMPure XP Beads (Beckman Coulter ...