Labshake search
Citations for Agilent :
1 - 5 of 5 citations for pcDNA5 FRT since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... a gift from Mark Cookson (Addgene plasmid #25361)77 was cloned into pcDNA5 frt/to by site-directed mutagenic removal of a single LRRK2 internal HpaI site (Quikchange, Stratagene), followed by HpaI/Eco53KI digest ...
-
bioRxiv - Molecular Biology 2022Quote: ... E197A and D199A were introduced in the parental pcDNA5/FRT/TO-DDX39B plasmid (Galarza- Munoz et al., 2017) using QuickChange Lightning Mutagenesis Kit (Agilent Technologies). All constructs and mutations were confirmed by Sanger sequencing ...
-
bioRxiv - Cell Biology 2022Quote: ... Full-length GFP-LRRK2 harboring an R361A mutation was generated by site-directed mutagenesis of pcDNA5 FRT/TO GFP-LRRK2 (DU13363) using the QuikChange Lightning Site-Directed Mutagenesis kit (Agilent Technologies, 210518) according to the manufacturer’s protocol and the following mutagenic primers ...
-
bioRxiv - Developmental Biology 2021Quote: ... a BirA-2A-Cherry-SV40pA-FRT-Kan-FRT cassette was PCR-amplified using Herculase II fusion DNA polymerase (Agilent Technologies) and recombined into the first coding exon of tcf21 within the DKEYP 79F12 BAC clone as previously described (Weinberger ...
-
bioRxiv - Cell Biology 2021Quote: ... W224A mutants were obtained in the pcDNA5 His PC TEV Arpin plasmid using QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent). ORFs encoding Arpin WT and mutant forms were subcloned between FseI and AscI sites into custom-made pcDNAm FRT PC GFP ...