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Citations for Agilent :
1 - 42 of 42 citations for PGK Promoter Lin28 Lentivirus since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2021Quote: The core promoter library was synthesized by Agilent technologies through a limited licensing agreement as 200bp oligonucleotides including flanking sequences for cloning ...
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bioRxiv - Physiology 2022Quote: ... and cloned into a pBlue Script containing the H1 promoter (Agilent). The casettes were inserted into a lentiviral vector pRRLsin-PGK-eGFP-WPRE (Addgene plasmid # 12252 ...
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bioRxiv - Plant Biology 2024Quote: ... The promoter fragments were cloned in the binary vector pBI121 (Stratagene) by a two steps reaction ...
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bioRxiv - Microbiology 2024Quote: AmpC promoter was amplified by Herculase II Fusion DNA Polymerase (Agilent) using isolated genomic DNA as template and F-AmpC Prom ...
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bioRxiv - Molecular Biology 2020Quote: ... Mutant plasmids for yeast promoter analyses were constructed by Quikchange mutagenesis (Stratagene) following adaptation for use of Phusion DNA polymerase (NEB ...
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bioRxiv - Genomics 2023Quote: ... The cells were then transduced with guide lentivirus using Bravo automated liquid handler from Agilent in three different replicates and incubated at 37°C ...
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bioRxiv - Genetics 2023Quote: ... Promoter capture Hi-C was performed using a custom-made RNA capture bait system (Agilent Technologies) consisting of 39,021 individual biotinylated RNAs targeting the ends of 22,225 promoter-containing mouse HindIII restriction fragments ...
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bioRxiv - Neuroscience 2021Quote: ... attached to the U6 promoter and subcloned into pBluescriptII SK(+) vector (Stratagene #212205, La Jolla, CA, USA), as described previously (Matsuzaki et al. ...
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bioRxiv - Developmental Biology 2024Quote: ... Promoter-specific Capture Hi-C was performed using the SureSelect XT Library Prep Kit ILM (Agilent Technologies), which included a custom-designed biotinylated RNA bait library and paired-end blockers ...
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bioRxiv - Biochemistry 2024Quote: The UAS+promoter DNA template was prepared by PCR using Herculase II Fusion DNA Polymerase (Agilent #600675) from the SB649 plasmid92 and upstream primer 5′-biotin-TTGGGTAACGCCAGGGT-3′ (IDT ...
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bioRxiv - Genetics 2021Quote: ... cloned into an expression plasmid driven by CMV promoter and modified using QuikChange Site-Directed Mutagenesis Kit (Agilent). For each affinity purification ...
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bioRxiv - Pathology 2021Quote: ... The promoter DNA was amplified with primers containing Nhe1 and Xho1 sites using Pfu Ultra II polymerase (Agilent) to generate the 0.7 kb truncated form in the pGL3-basic vector ...
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bioRxiv - Neuroscience 2023Quote: ... The CLEAR mutant promoters were generated using site-directed mutagenesis (QuikChange II XL Site-Directed Mutagenesis Kit, Agilent). Renilla plasmid was transfected at 1/20 the amount of the other plasmids ...
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bioRxiv - Genomics 2024Quote: Ligation products containing promoters were captured using the SureSelectXT Target Enrichment System for the Illumina Platform (Agilent Technologies), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... SVBP KO and TTL KO embryos were infected with eGFP containing lentivirus and fixed at DIV18 prior to mounting with DAKO antifade mounting reagent (Agilent S3023). Dendritic segments visualized by eGFP were obtained using an inverted microscope (Axio Observer ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We produced the promoter inserts by performing error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). We used the plasmid constructs with MG1655 promoter variants cloned into them as template DNA for the error-prone PCR aiming to achieve approximately 1.5 SNPs per promoter sequence ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We produced the promoter inserts by performing error-prone PCR using the GeneMorph II Random Mutagenesis Kit (Agilent Technologies). We used 25 ng of the plasmid construct with the MG1655 lacZ promoter variant cloned into it as a template DNA for the error-prone PCR to achieve approximately 1.5 SNPs per variant sequence ...
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bioRxiv - Neuroscience 2023Quote: AAV plasmids carrying cDNA for hIL-6 downstream of a CMV promoter were co-transfected with pAAV-RC (Stratagene) encoding the AAV genes rep and cap ...
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bioRxiv - Genomics 2022Quote: Enrichment of promoter-containing ligation products was performed using SureSelectXT Target Enrichment System for the Illumina Platform (Agilent Technologies) as instructed by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... the full-length legK1 gene was cloned under the control of its own promoter into pBC-KS (Stratagene, 212215) using SacI and KpnI restriction enzymes ...
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bioRxiv - Developmental Biology 2022Quote: ... A 5.5-kb fragment of the ptf1a promoter sequence was retrieved at HindIII and SmaI sites and inserted into a pBluescript SK (+) (Stratagene) shuttle vector upstream of the membrane-targeting mCherry-CAAX sequence ...
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bioRxiv - Systems Biology 2020Quote: Block 3-6s for the motif-rich core regions of our synthetic promoter constructs were amplified from a library synthesized by Agilent Technologies (LeProust et al. ...
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bioRxiv - Biochemistry 2022Quote: ... BioID2-tagged variants were generated by introducing a BamHI site between the 8xHis and MBP tags in the 8xHis-MBP-pFastBac1 modified with a CMV promoter vector by Quikchange Lightning site-directed mutagenesis (Agilent) and cloning BioID2 into the BamHI sites using In-Fusion EcoDry cloning according to manufacturer protocols.
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bioRxiv - Evolutionary Biology 2022Quote: We isolated the plasmids containing the three segregating lacZ promoter variants from the MG1655 strain using StrataPrep Plasmid Miniprep Kit (Agilent). We also isolated the promoter- less vector pMV001 the same way ...
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bioRxiv - Genomics 2021Quote: The different Sen1 HD versions were fused to a C-terminal His8-tag coupled to Vibrio cholerae MARTX toxin cysteine protease domain (61)and expressed from the T7 promoter (see plasmids in table S6) in Escherichia coli strain BL21 (DE3) CodonPlus (Stratagene). Overexpression was induced by growth in auto-inducing medium (62 ...
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bioRxiv - Biochemistry 2021Quote: ... and transfected with a DNA mixture containing either the 4xNFAT or the 7xAP-1 promoter-Firefly luciferase reporter (100 ng; Stratagene), along with Renilla luciferase (50 ng of pGL4.70[hRluc] or 1 ng of pRL-null ...
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bioRxiv - Plant Biology 2022Quote: ... was used as a template to create different promoter mutants using the QuikChange II kit according to manufacturer’s protocol (Agilent Technologies). Mutagenic mismatch primers are listed in Tab ...
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bioRxiv - Biochemistry 2023Quote: ... of C2C12 cells expressing UCP1 under the control of the doxycycline-inducible promoter (TRE3G) were measured with Seahorse XFe24 analyzer (Agilent). Cells were seeded at 4 x 104 cells per well in XFe24 cell culture microplates (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... The gene sequence was PCR-amplified with primers to introduce T7 promoters on both ends (see Table S2, Oligonucleotides tab) and cloned into pSC-A-amp/kan (Stratagene) vector ...
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bioRxiv - Microbiology 2024Quote: ... The amplified vectors were digested with Dpn1 and 1 µL of each (vector and promoter region insert) were transformed into ultracompetent XL-10 Gold cells (Agilent) for in vivo assembly ...
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bioRxiv - Plant Biology 2024Quote: ... together with a C-terminal 3xFLAGtag (MoClo toolbox; pICSL50007) (Engler et al., 2014) behind the SP6 promoter into the pSPUTK expression vector (Stratagene) that was made Golden Gate compatible as described (Kerstens et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... The deletion constructs of MAD2 as well as BUB1B promoters were generated by omitting one of the estrogen response element (ERE) using Quikchange II site-directed mutagenesis kit (Agilent).
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bioRxiv - Synthetic Biology 2020Quote: ... the eight genes flanked with the same promoters and terminators were cloned into the 2μ-based high-copy plasmids pESC-URA-USER and pESC-HIS (#217451, Agilent Technologies), resulting in pESC-URA-USER harboring CYP79A2 ...
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bioRxiv - Genomics 2022Quote: ... and T7-Mutagenesis-primer-F and T7-Mutagenesis-primer-R to replace the “G” following the T7 promoter sequence with an “A” by the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, 210518). T7-5’UTR fragment was PCR-synthesized using the mutagenesis-modified pcDNA3.3-eGFP and T7-5’UTR-primer-F and T7-5’UTR-primer-R ...
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bioRxiv - Molecular Biology 2021Quote: ... Enzymatically-dead zDHHC3 mutant transgenic mice overexpressing zDHHC3DHHS in cardiomyocytes were generated by site-directed mutagenesis of the α-MHC-Zdhhc3 promoter-transgene construct using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) to encode a mutation of Cys-157 in mouse zDHHC3 protein to Ser ...
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bioRxiv - Cancer Biology 2023Quote: Deletions of SP1 sites on human MGMT promoter were performed using Quick-Change XL Site-Directed Mutagenesis kit (Agilent Technologies, USA).34 The mutated promoters were further cloned into pGL3 basic vector ...
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bioRxiv - Molecular Biology 2024Quote: The ssDNA libraries were converted into dsRNA by PCR using primers in T7 promoter _ Mega Fwd and Moe Rev sequences and the Taq Precision Plus enzyme (Agilent Technologies). Cy5-CTP/Cy5-UTP (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: ... PCR was performed using primers containing the T7 promoter sequence (on both forward and reverse primers) with PfuUltra II Fusion Hotstart DNA Polymerase (Agilent Technologies, #600672). PCR products with the expected size was separated using 1% agarose gel ...
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bioRxiv - Neuroscience 2023Quote: ... The transfer plasmid was constructed by replacing the CMV promoter of an AAV backbone plasmid (pAAV-CMV, Stratagene, San Diego, CA, USA) with a human Syn promoter ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids harboring mutated sRNA alleles under the control of the PLlac promoter were constructed using the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies, Santa Clara, CA) with oligonucleotides that contain mismatched bases at the desired locations (Table S2 ...
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bioRxiv - Cancer Biology 2021Quote: Deletion mutants of two TCF4 binding sites (TBE1 and TBE2) on the STUB1 promoter were generated as previously performed [25] using the Quickchange XL Site directed mutagenesis kit (Agilent technologies, Santa Clara, CA, USA) as per the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Deletion of TBE consensus sites in the FOXM1 promoter was accomplished using the QuickChange XL Site-Directed Mutagenesis44 kit (Agilent Technologies, Santa Clara, CA, USA) by following manufacturer’s instructions and was then cloned into the pGL3 basic vector ...