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Citations for Agilent :
701 - 750 of 824 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed for 5 minutes using 1X Wash Buffer and then blocked by adding 8 drops of Protein Block Serum-Free (Agilent). Primary antibodies were diluted in diluent (Dako ...
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bioRxiv - Cell Biology 2020Quote: ... One hour before the analysis the cells were washed twice in phenol red and bicarbonate free DMEM Seahorse Media (SM) (part nb 103575-100, Agilent) containing 25 mM glucose (Cat nb ...
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bioRxiv - Biochemistry 2022Quote: ... according to the construct used by the Xia lab [48] was generated by the restriction free cloning method [54] and site-directed mutagenesis (QuickChange Site-Directed Mutagenesis Kit, Agilent). Expression and purification were carried out as described in Tang and Xia [48].
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bioRxiv - Plant Biology 2022Quote: His6-MBP-PFA-DSP protein fusions or free His8-MBP were expressed in Escherichia coli BL21 CodonPlus (DE3)-RIL cells (Stratagene). Overnight bacterial cultures were inoculated 1:1000 into fresh 2YT medium (1.6 % tryptone ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were washed with TBST (Tris Borate Saline Tween-20) and then blocked with Protein Block Serum-Free (Ref: No X0909, Dako) for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... RNA samples were eluted from columns using 30 μl of RNase-free water and their concentrations were determined or spectrophotometrically by A260 (Nanodrop–ND1000) or using a 2100 Bioanalyzer (Agilent) resulting in RNA integrity number ≥8.
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bioRxiv - Biochemistry 2021Quote: The fluorophore-labeled single-stranded DNA oligonucleotide was separated from unlabeled oligonucleotide and unincorporated free fluorophore by C18 reverse-phase HPLC chromatography (Poroshell 120 EC-C18, Agilent). Labeled fractions were pooled and ethanol precipitated as described above and resuspended in aqueous solution (10mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... Sensor plates were calibrated overnight in a CO2-free incubator at 37°C using 1 mL/well of XF calibrant solution (Agilent). After polarization ...
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bioRxiv - Pathology 2020Quote: ... Sections were allowed to thaw at RT and thereafter blocked for > 60 min at RT with blocking-buffer (serum-free protein blocking solution, DAKO), supplemented with 0.2% Triton X-100 (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... free floating sections were blocked with 3% donkey serum and incubated overnight with either rabbit anti-GFAP (1:10000, Dako), rat anti-CD68 (1:200 ...
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bioRxiv - Systems Biology 2021Quote: ... The ethanol was removed carefully and the resulting RNA extract was resuspended in 10 μL RNase-free water for immediate analysis (RNA 6000 Nano kit, Bioanalyzer instrument and the Bioanalyzer 2100 software, Agilent).
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bioRxiv - Genomics 2021Quote: ... The cfDNA concentration was determined using an Agilent 4200 TapeStation System with the Cell-free DNA ScreenTape Analysis assay (Agilent).
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bioRxiv - Cell Biology 2020Quote: ... magnesium-free PBS and irradiated on ice with 150 mJ/cm2 of UVC (254 nm) in a Stratalinker 2400 (Agilent). Cells were collected in ice-cold PBS ...
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bioRxiv - Genomics 2022Quote: ... The cfDNA concentration was determined using an Agilent 4200 TapeStation System with the Cell-free DNA ScreenTape Analysis assay (Agilent).
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bioRxiv - Microbiology 2022Quote: ... HNO3 using metal free water and the intracellular Zn and Mn content determined using a 7900 ICP Mass Spectrometer (Agilent). Metal concentrations were normalized to total protein content determined by the bicinchoninic acid (BCA ...
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bioRxiv - Neuroscience 2022Quote: Adeno-associated viral (AAV) vector serotype 2 was prepared using an AAV Helper-Free system (Agilent Technologies, Santa Clara, CA) as described previously (Kato et al. ...
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bioRxiv - Cancer Biology 2022Quote: The sensor cartridge plate was hydrated with sterile distilled water overnight at 37°C in a CO2-free incubator and was replaced with a calibrant solution (Agilent) for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... The elution was in 10-20 µL of RNase-free water and the RNA profiling and quantification was assessed by Agilent 2100 Bioanalyzer and RNA 6000 Pico Kit (5067-1513) ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were then assessed for appropriate amplicon size and complete elimination of free primer peaks using the High Sensitivity ScreenTape Assay on the Tape Station2200 (Agilent) and quantified using the qPCR-based quantification in order to ensure only NGS-compatible amplicon was quantified using the Library Quant ROX Low Kit (Kapa Biosystems ...
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bioRxiv - Immunology 2024Quote: ... and MØ cell culture media was replaced with FCS-and bicarbonate-free DMEM medium supplemented with 4.5 mg ml-1 D-glucose and 2 mM glutamine (Agilent, USA) for another 60 min incubation at 37°C without CO2 ...
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
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bioRxiv - Immunology 2023Quote: ... Antigen retrieval was then performed for 20 minutes at 95°C using Lecia Epitope Retrieval Buffer 2 followed by treatment with Dako serum-free protein block (X090930-2, Agilent Dako) for 15 minutes to prevent non-specific binding of the antibody ...
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bioRxiv - Neuroscience 2023Quote: ... woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) and SV40 polyadenylation signal was assembled using a modified helper-free system (Stratagene) as a serotype 2/1 (rep/cap genes ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous peroxidases were blocked by incubation with 3 % H2O2 and then blocked for 1 h (Dako Serum-free protein block). Sections were then incubated with the 1st primary antibody (FABP7 ...
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bioRxiv - Physiology 2024Quote: ... the cells were washed twice in phenol red and bicarbonate free DMEM Seahorse Media (SM) (part. no.: 103575-100, Agilent) containing 25 mM glucose (Cat no ...
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bioRxiv - Physiology 2024Quote: ... RNA was reconstituted in nuclease-free water (20-50 µL) and RNA quantity and quality was assessed using a bio-analyzer (2100 Bioanalyzer, Agilent Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... An increase of fluorescent signal from free AMC (λex 365nm; λem 440 nm) was monitored by using BioTek Synergy H1 (Agilent) at room temperature ...
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bioRxiv - Genomics 2024Quote: ... The slides were dried for 15 min and then cover slipped using 1.5 mm thick cover glass and Cytoseal Mountant XYL (ProSciTech, 1A013-XYL-118) or toluene-free mounting media (Dako, CS705). For post-CosMx (STAMP-X-CP/CP/C ...
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bioRxiv - Cancer Biology 2024Quote: ... Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays” by Brad Larson (Agilent Technologies, Inc., USA).
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bioRxiv - Molecular Biology 2021Quote: ... approximately 8 µg of protein was injected on a Zorbax 300SB-C18 column (5 µm, 300Å, 1×250mm IDxL; Agilent Technologies) and separated using a 30 min gradient from 5% to 80% solvent B at a flow rate of 100 µl/min (solvent A ...
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bioRxiv - Microbiology 2022Quote: ... and Sialyl-Lewis x (sLex/CD15s) were detected with 2 µg/ml of mAbs NS-1116-19.9 (Dako, Agilent Technologies, USA) and CSLEX-1 (Becton Dickinson ...
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bioRxiv - Cell Biology 2021Quote: ... washed three times in PBST containing 10 µg/ml DAPI for 5 minutes and mounted with a coverslip in fluorescent mounting media (Dako, S3023). All images were taken using a Zeiss LSM 710 confocal microscope and quantified manually using ImageJ software ...
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bioRxiv - Microbiology 2023Quote: ... approximately 2 µg of protein was injected on a Zorbax Poroshell 300SB-C8 column (5 µm, 300Å, 1×75mm IDxL; Agilent Technologies) and separated using a 15 min gradient from 5% to 80% solvent B at a flow rate of 100 µl/min (solvent A ...
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bioRxiv - Plant Biology 2023Quote: ... One hundred micrograms of precipitated protein sample were digested with trypsin and 30 µg of the resulting peptides desalted with PepClean C-18 Spin Columns (Agilent Technologies) according to manufacturer recommendations as previously described (Martinez-Marquez et al ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes were washed in TBS-t and subsequently incubated with goat anti-rabbit IgG horseradish peroxidase-conjugated secondary antibody (0.5 µg/ml; Dako, VIC, Australia) or rabbit anti-Goat IgG horseradish peroxidase-conjugated secondary antibody (0.125 µg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... 15 min) before incubation with either rabbit anti-mouse IgG (1.3 µg ml−1, 1% BSA in PBST; Agilent, P026002-2), goat anti-rabbit IgG (250 ng ml−1 ...
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bioRxiv - Cell Biology 2024Quote: ... pmol/min/µg of protein) and extracellular acidification rate (ECAR, mpH/min/µg of protein) using the Seahorse XF96 extracellular flux analyser (Seahorse Bioscience), respectively ...
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bioRxiv - Bioengineering 2024Quote: ... Primary (FHL2 2 µg/mL and Ki67 2 µg/mL) and secondary antibodies (same as for in vitro immunofluorescence staining) were diluted in Dako antibody diluent (Dako, Germany). Washing steps were done in Dako washing buffer ...
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bioRxiv - Cell Biology 2024Quote: ... were seeded at 6,000 cells per well in 80 µL of MEM media (MEM 10% FBS + 1% P/S + 1 mM Sodium Pyruvate + 2 mM Glutamax + 1% nonessential amino acids + 50 µg/mL uridine) in a Seahorse 96-well cell culture plate (Agilent, USA). After 24 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... was applied for 30 min at a concentration of 0.108 µg/mL at room temperature using Antibody Diluent (S302283-2, Agilent Technologies Inc). Detection was performed using the Bond Polymer Refine Kit (DS9800 ...
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bioRxiv - Molecular Biology 2020Quote: ... were induced with 2μg/mL doxycycline for 6 hours prior to crosslinking at 100mJ/cm2 at 4°C in a Stratalinker 1800 (Stratagene). Cells were then processed according to the eCLIP protocol for input and immunoprecipitated samples until cDNA was obtained ...
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bioRxiv - Systems Biology 2021Quote: ... The staining was performed according to the manufacturer’s procedure with EnVision G|2 Doublestain System Rabbit/Mouse (DAB+/Permanent Red) kit (Dako/Agilent K5361) on the Dako Autostainer ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant viruses were produced in 293T cells by cotransfecting pLPCX plasmids with pVPack-GP and pVPack-VSV-G packaging plasmids (Stratagene). The pVPack-VSV-G plasmid encodes the vesicular stomatitis virus (VSV ...
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bioRxiv - Cell Biology 2020Quote: ... pGEX-PBD (a gift from G. Bokoch70) was transformed into BL21(DE3)-competent bacteria (Agilent Technologies, Santa Clara, CA, USA) and grown in a shaker flask at 225 rpm and 37°C until an optical density of 1.0 at 600 nm was achieved ...
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bioRxiv - Cancer Biology 2020Quote: ... The secondary antibodies used for western blotting were horseradish peroxidase (HRP)-conjugated antibody against mouse immunoglobulin G (IgG) and (HRP)-conjugated antibody against rabbit IgG from Dako.
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bioRxiv - Plant Biology 2021Quote: ... The filtrate was passed through a 1 g phase C18 silica-bonded non-polar column (HF Mega Bond Bond Elut C18, Agilent, 5301 Stevens Creek Blvd ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... OCI-AML3 and NALM6 cells stained with PBS containing 0.1 μg/mL acridine orange were evaluated for GFP and RFP channels using a fluorescent microscope (Lionheart FX Automated microscope; Agilent BioTek Instruments Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with 1 μg/mL recombinant anti-PSD95 antibody (Rabbit Fc fusion, NanoTag Biotechnologies #N3783) diluted in Dako REAL Antibody Diluent (S2022, Agilent) for 1 h at RT ...
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bioRxiv - Cell Biology 2024Quote: ... 3 µg of DNA were sonicated to obtain 150-200 bp fragments and the efficiency was checked by the Bioanalyzer (Agilent). DNA libraries were prepared using NEBNext® reactives (New England Biolabs) ...