Labshake search
Citations for Agilent :
651 - 700 of 1459 citations for 3 Chloro 5 fluoro 4' morpholinomethyl benzophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... LC-MS experiments were carried out on an Agilent 1100 Series LC with a Poroshell 120 EC-C18 column (100 × 3 mm, 2.7 µm, Agilent Technologies) and an Agilent G1956B Series Single Quadripole MS in positive ion mode for mass detection ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were resuspended in FACS staining buffer (3% FBS in PBS) and analyzed by flow cytometry on a Novocyte 3000 (Agilent). Flow cytometry results were analyzed using NovoExpress (version 1.5.6).
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bioRxiv - Microbiology 2023Quote: ... solution and 3 μl of each sample were subjected to high-resolution liquid chromatography-mass spectrometry (LC-MS) analysis (Agilent iFunnel 6550 quadrupole time-of-flight (QTOF) ...
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bioRxiv - Microbiology 2023Quote: ... along with gene-specific primers listed in Supplemental Table 3 and qPCR was performed in technical triplicate on a StepOnePlus (Stratagene). Ct values of target genes were normalized to β-actin and relative gene expression levels between conditions were calculated via the ΔΔCt method ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were acidified with trifluoroacetic acid (TFA) to lower the pH below 3 and desalted on reversed phase (RP) C18 OMIX tips (Agilent). The tips were first washed 3 times with 100 µl pre-wash buffer (0.1% TFA in water/acetonitrile (ACN ...
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bioRxiv - Cell Biology 2023Quote: ... GEMs were used to generate barcoded cDNA libraries following the manufacturer’s protocol (Single Cell 3’ Reagent Kit v3.1, 10X Genomics) and quantified using the TapeStation High Sensitivity D5000 kit (Agilent, Germany). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were incubated in the following primary antibodies for 3 h at 24L: gastrin (1:1000, Cat #A0568, A0568, AB_2757270, Dako Agilent Technologies), Chromogranin A (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... of HIV-1 were amplified by PCR using KOD-Plus-Neo and pNL4-3 as a template and cloned into pBluescript II KS(-) (212208, Agilent). The MLV 5’-LTR (1 - 207 nt ...
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bioRxiv - Cell Biology 2023Quote: ... then washed 3 × 10minute and post-fixed with 0.1% PFA for 10minutes and mounted using fluorescent mounting medium (DAKO, USA).
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bioRxiv - Cell Biology 2023Quote: ... the HA epitope sequence was inserted immediately 3’ to the signal peptide sequence by site directed mutagenesis using the QuickchangeXL site directed Mutagenesis kit (Agilent) to obtain the following intermediate vector ...
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bioRxiv - Physiology 2023Quote: ... The cells were transfected with 100 ng of NF-κB firefly luciferase reporter plasmid p(NF-κB)3-Luc (Stratagene) and 10 ng of Renilla luciferase reporter plasmid pRL-RSV (Promega) ...
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bioRxiv - Physiology 2023Quote: ... Acetone was measured using an Agilent DB-35MS column (30 m 3 0.25 mm i.d. x 0.25 µm, Agilent J&W Scientific) installed in an Agilent 7890A gas chromatograph (GC ...
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bioRxiv - Molecular Biology 2023Quote: Size Exclusion Chromatography in line with Multi Angle Laser Light Scattering (SEC-MALLS) experiments for absolute mass determination were conducted at 25°C with a BioSEC-3 column (Agilent) equilibrated in 20 mM Na-phosphate pH 7.0 ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous peroxidases were blocked by incubation with 3 % H2O2 and then blocked for 1 h (Dako Serum-free protein block). Sections were then incubated with the 1st primary antibody (FABP7 ...
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bioRxiv - Physiology 2023Quote: ... and 4 μl of supernatant was injected into an HILIC column (HILIC Silica 3 μm, 2.1 × 150 mm, SN: 186002015; Atlantis) on a 1290 Infinity II LC System (Agilent Technologies) which is interfaced with a 6545 MS (Agilent Technologies) ...
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bioRxiv - Cell Biology 2023Quote: ... All sections were washed three times for 3 min with PBS and mounted with Dako Fluorescent Mounting Medium (Agilent, USA).
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bioRxiv - Neuroscience 2024Quote: ... blots were washed 3 times during 10 min with PBS-T and incubated with horseradish peroxidase-conjugated secondary antibody (Dako) for 1 h and washed again 3 times ...
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bioRxiv - Microbiology 2024Quote: ... These pellets were lysed and 3 µl samples were analyzed using Agilent InfinityLab Poroshell 120 HILIC-Z (Agilent 683775-924). The chromatographic separation employed two solvent phases ...
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bioRxiv - Synthetic Biology 2024Quote: By acidic methanolysis processed PHB (3-hydroxybutyrate methyl ester) was analyzed by gas chromatography (GC 6850, Agilent Technologies, Basel, Switzerland) equipped with a 7683B Series injector coupled to a flame ionization detector (FID) ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then washed 3 times with TBST for 10 min at room temperature and incubated with horseradish peroxidase coupled secondary antibodies (Dako anti-rabbit #P0448 or anti-mouse #P0447 ...
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bioRxiv - Microbiology 2024Quote: The separation was performed using a ZORBAX RRHD Eclipse Plus (C18, 3 × 50 mm, 1.8 μm; Agilent Agilent 959757-302) or a Poroshell 120 EC-C18 ...
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bioRxiv - Microbiology 2024Quote: The separation was performed using a ZORBAX RRHD Eclipse Plus (C18, 3 × 50 mm, 1.8 μm; Agilent Agilent 959757-302) or a Poroshell 120 EC-C18 ...
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bioRxiv - Immunology 2024Quote: ... cDNA and libraries were made using the Lexogen QuantSeq 3’ mRNA-seq FWD library prep kit and quality was assessed by Agilent High Sensitivity DNA kit on a Bioanalyzer (Agilent) ...
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bioRxiv - Biochemistry 2024Quote: ... The effect of PFKFB2/3 inhibitors on glycolysis was determined by glycolysis stress test utilizing Seahorse XFe24 Extracellular Flux Analyzer (Agilent) measuring extracellular acidification rate (ECAR ...
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bioRxiv - Bioengineering 2024Quote: ... sections were washed in PBS three times for 3 minutes each before being incubated with DAKO Rabbit/Mouse HRP Kit-provided HRP (Mouse-K4001, Rabbit-K4003; Dako) for 30 minutes at room temperature ...
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bioRxiv - Synthetic Biology 2021Quote: ... The extract was purified on an Eclipse XDB-C18 (5 µm, 9.4 mm × 250 mm, Agilent) on an Agilent 1200 HPLC using a linear gradient from 15 to 45% acetonitrile over 42 min with water as the aqueous mobile phase after an initial increase of 0 to 15% acetonitrile over 1.5 min at flow rate of 4 mL/min ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μg pooled RNA was quality tested on an ABI2000 bioanalyzer (Agilent Technologies, Sta. Clara, CA), labeled ...
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bioRxiv - Biochemistry 2021Quote: ... separations were carried out on an AdvanceBio Peptide Map Guard (2.1×5 mm, 2.7μm, Agilent technologies) and AdvanceBio Peptide Map column (2.1×150 mm ...
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bioRxiv - Biochemistry 2020Quote: ... the peaks separation occurred on Zorbax Eclipse Plus C18 5 µm (4.6×250 mm) column (Agilent) in 0.1 M TEAA buffer system ...
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bioRxiv - Cancer Biology 2020Quote: ... Following antigen retrieval by autoclaving for 5 min in DAKO antigen retrieval solution (DAKO, Carpenteria, CA) the sections were washed twice in TBS buffer ...
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bioRxiv - Microbiology 2021Quote: ... equipped with a 5% phenylmethyl silicone capillary column and a mass spectrometer (model 5975C; Agilent Technologies). Helium was used as carrier gas ...
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bioRxiv - Microbiology 2020Quote: ... equipped with an Eclipse XDB-C18 reverse-phase column (5 μm; 4.6 × 250 mm; Agilent, USA) and an LCQ Deca XP Max MS instrument (Thermo Finnigan ...
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bioRxiv - Cancer Biology 2021Quote: ... Eluates were desalted using C18 cartridges (5 µL bed volume, Agilent Technologies, cat. no. 5190–6532) on an automated liquid handler as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... then washed four times for 5 min with PBS and mounted with Fluorescence Mounting Medium (Dako). Measurement of the percentage of Evans Blue Dye uptake was performed counting the number of Evan’s blue dye positive fibers on total muscle section reconstructions ...
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bioRxiv - Cell Biology 2021Quote: ... then washed four times for 5 min with PBS and mounted with Fluorescence Mounting Medium (Dako). For Nfix-F4/80 double immunolabeling ...
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bioRxiv - Cell Biology 2022Quote: ... residual PBS was removed and cells were blocked with 5% Normal Swine Serum (NSS, Dako, X0901) in PBS for 20 min ...
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bioRxiv - Physiology 2022Quote: ... and rotenone and antimycin A (5 μM) according to the manufacturer’s instructions (Agilent Technologies, 103015-100).
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bioRxiv - Cancer Biology 2022Quote: ... Fluorescence was monitored for 17 h using a Cytation 5 device (BioTek, Agilent, Winooski, VT, USA). Excitation was provided by a monochromator ...
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bioRxiv - Neuroscience 2021Quote: ... Mobile phase A consisted of water with 10 mM ammonium acetate + 5 μM medronic acid (Agilent), pH 9 ...
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bioRxiv - Microbiology 2022Quote: ... using a C8 reverse phase micro-column (Zorbax 300SB-C8, 5μm, 5 × 0.3 mm, Agilent Technologies). The sample was then eluted with 70% of mobile phase B (flow rate of 50 μl/ min ...
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bioRxiv - Developmental Biology 2022Quote: ... Slides were visualized using a Cytation 5 microscope and Gen5 imaging software (Agilent, Santa Clara, CA).
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bioRxiv - Immunology 2021Quote: ... - Kinetex EVO (5 μm, 100 Å)) on a HPLC system (Agilent, LC 1260 Infinity II, Agilent). A two-step gradient was applied ...
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bioRxiv - Microbiology 2020Quote: 5 μl of each sample was injected into XCT6330 LC/MSD ultra trap system (Agilent Technologies) equipped with a Nucleosil 100 C18 column (3 μm x 100 mm × 2 mm internal diameter ...
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bioRxiv - Cell Biology 2021Quote: ... Support fibers were generated from 5 wt% solutions of polystyrene (MW: 15,000,000 g/mol, Agilent Technologies) dissolved in xylene ...
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bioRxiv - Plant Biology 2022Quote: ... on a J&W DB-5 ms column (30 m × 0.25 mm × 0.25 μm, Agilent Technologies) and analyzed using a transmission quadrupole mass spectrometer (Agilent 5975 C series ...
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bioRxiv - Neuroscience 2023Quote: ... Plates were consecutively incubated with: rabbit anti-total tau antibody (K9JA, Dako #A0024, 5 μg/ml), anti-rabbit secondary antibody conjugated with biotin (Invitrogen #A16114 ...
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bioRxiv - Microbiology 2023Quote: ... cells were grown with agitation at 37°C in an imaging multimode reader (Cytation 5, Agilent). Cell growth was monitored every 5 min by measuring absorbance at 600 nm ...
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bioRxiv - Zoology 2024Quote: ... We used an HP-5 column (30 x 0.25 mm ID X 0.25 μm, Agilent, US) with nitrogen (2 ml/min ...
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bioRxiv - Neuroscience 2024Quote: ... mut-shM4 (Table 5) and the QuikChange Multi Site-Directed Mutagenesis kit (200514; Agilent; Manufacturer Instructions) in several assays until all the mutations were incorporated ...