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Citations for Calbiochem :
1 - 50 of 50 citations for ssc mir 299 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... TdT Colorimetric FragEL DNA Fragmentation Detection Kit (QIA33 TdT FragEL; Calbiochem) was used for TUNEL staining ...
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bioRxiv - Neuroscience 2020Quote: TUNEL staining was performed using the FragEL DNA Fragmentation Detection Kit from Calbiochem (EMD Millipore). 10-day old adult fly brains were dissected in PBST and then fixed in 4% paraformaldehyde for 20 minutes on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were then incubated in a wash buffer consisting of 2X nuclease free SSC and 10% deionized formamide (Calbiochem 4610) for 5 minutes at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Then preparations were incubated for 15 min with hybridization buffer prepared using 2x saline-sodium citrate (SSC) solution / 10 % deionized formamide (4610-OP, Calbiochem). Hybridization with Stellaris FISH probes was done in a total volume of 50 μl hybridization buffer containing 50 μg competitor tRNA from E.coli (10109541001 ...
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bioRxiv - Developmental Biology 2022Quote: ... we performed terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay according to the manufacturer’s instructions (FragEL™ DNA Fragmentation Detection Kit, Colorimetric-Klenow Enzyme, Calbiochem). Briefly ...
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bioRxiv - Developmental Biology 2020Quote: Apoptotic chondrocytes in frontal knee joint sections were assessed for Terminal deoxynucleotidyltransferase-mediated dUTP Nick End Labeling (TUNEL) using DNA Fragmentation Detection Kit (QIA33-1KT, Calbiochem) and following manufacturer’s instructions.
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bioRxiv - Biophysics 2020Quote: ... The cells were washed three times in the washing buffer 1X of OxyDNA Assay Kit (500095, Calbiochem) and incubated overnight at 37°C in FITC-conjugated anti 8-oxoguanine (500095 ...
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bioRxiv - Biophysics 2020Quote: ... Cells were stained with Annexin-V-FITC and propidium iodide as indicated in the manual of the annexin V-FITC detection kit (CalbioChem, Darmstadt, Germany) and fluorescence was measured using a FACS Calibur flow cytometer (Becton-Dickinson ...
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bioRxiv - Physiology 2022Quote: ... then washed three times (each time 10 min) and subsequently mounted with Fluorsave medium (Calbiochem). The controls were the sections where primary antibody was omitted ...
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bioRxiv - Bioengineering 2021Quote: NO detection was performed by incubating microvessels with 5μM DAF-2 (Calbiochem) for 30 minutes at 37°C prior to imaging at 2-minute intervals ...
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bioRxiv - Evolutionary Biology 2021Quote: ... blocked (200 μL, PBS 1% BSA wt/vol, Calbiochem #12659-100GM, 3 h, RT) and washed (3x ...
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bioRxiv - Immunology 2020Quote: ... blocked (200 µL, PBS 1% BSA wt/vol, Calbiochem #12659-100GM, 3 h, RT) and washed (3x ...
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bioRxiv - Cell Biology 2023Quote: ... in PBS for 5 min at RT and cells were mounted in Mowiol 4-88 (Calbiochem) solution (Wei and Seemann ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed three times with PBS and mounted with Mowiol (Calbiochem). Z-stack images (spacing 0.3 μm ...
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bioRxiv - Neuroscience 2019Quote: ... diluted in KPBS containing 0.3 % Triton X-100 and incubated overnight at RT in cFos rabbit polyclonal antiserum (1:20,000; Calbiochem) diluted in KPBS containing 1 % NGS and 0.3 % Triton X-100 ...
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Tight junction membrane proteins regulate the mechanical resistance of the apical junctional complexbioRxiv - Cell Biology 2023Quote: ... the cells were washed three times with PBS and mounted using FluoroSave™ Reagent (Calbiochem; #345789). For STED imaging ...
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bioRxiv - Neuroscience 2021Quote: Pharmacological agents were used in the following concentrations and time courses: cycloheximide (Calbiochem, 0.2 µg/µl, 24hrs), bicuculline (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... the monolayers were washed three times with PBS and the glass coverslips were mounted using Fluorsave (Calbiochem).
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bioRxiv - Physiology 2020Quote: ... The remaining precipitate was washed with cold acetone for three times (All reagents from Sigma-Aldrich and Calbiochem). The protein was redissolved in 8M urea and the protein concentration was determined with BCA Protein Assay Kit (P0009 ...
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bioRxiv - Immunology 2020Quote: ... Fixed cells were washed three times in PBS and permeabilized by incubating them in PBS containing 5% non-fat milk and 0.05% saponin (Calbiochem). Coverslips were placed in primary antibody for 1 hour then washed 3x in PBS and placed in secondary antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were then washed two times for 10 min in PBST before mounting using Mowiol (30% wt/vol glycerol, 3.87 mM Mowiol [Calbiochem, 475904] ...
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bioRxiv - Bioengineering 2022Quote: ... The microfluidic devices were then washed three times with PBS and permeabilized for 20 min with 0.1% Triton X-100 (Calbiochem) diluted in PBS ...
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bioRxiv - Microbiology 2023Quote: ... Wells were then washed six times with PBST and added with human fibrinogen (500 ng/well, PLG depleted; Calbiochem) and uPA (1 U/well) ...
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bioRxiv - Neuroscience 2021Quote: Pharmacological agents were used in the following concentrations and time courses: cycloheximide (Calbiochem, 0.2 μg/μl, 2, 4 or 8h), dexamethasone (Invivogen ...
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bioRxiv - Immunology 2022Quote: ... Cells were left unstimulated or stimulated for indicated time at 37 °C with PMA (10 ng/ml; Alexis) and ionomycin (1 μM; Calbiochem) or solvent (DMSO) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 3 times with PBS and fixed to a slide using 5 μL of Mowiol (4-88 Reagent, Calbiochem). Fluorescent images were acquired with a Leica Dmi8 microscope (Leica Microsystems).
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bioRxiv - Neuroscience 2023Quote: ... cells were washed with PBS three times and the coverslips mounted using FluorSave (Millipore Calbiochem; Cat Number: 34-578-9).
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bioRxiv - Neuroscience 2023Quote: ... After washing three times for 10 min in TBST the sections were mounted on glass slides using Mowiol (Calbiochem/ MerckMillipore), glass covered and stored at 4°C.
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bioRxiv - Molecular Biology 2023Quote: ... Fixed cells were washed three times with a wash solution (nuclease-free water with 300 mM NaCl, 30 mM sodium citrate (Calbiochem 567446), 10% (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... cells were rinsed two times with pre-warmed medium and incubated for 30 min at 37°C with rabbit anti-GluA1 (1/150; Calbiochem/Millipore, RRID: AB_564636) in culture medium ...
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bioRxiv - Immunology 2023Quote: ... 2 µl were used as PCR template for amplification with the KOD Polymerase (Calbiochem, 71086-3). The PCR products were cloned into the pI.18 vector ...
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bioRxiv - Cell Biology 2022Quote: ... a proteoExtract protein precipitation kit (Calbiochem, #539180) was used followed by mass spectrometry analysis (Taplin Biological Mass Spectrometry Facility ...
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bioRxiv - Molecular Biology 2019Quote: The ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem) was used to generate lysates from the cytosolic ...
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bioRxiv - Immunology 2021Quote: ... then precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem). Equal amounts of protein for each set of samples (e.g ...
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bioRxiv - Immunology 2022Quote: ... prior to protein precipitation (Calbiochem Proteo-Extract precipitation kit). Precipitants were resuspended in 1% SDS and quantified by BCA assay for normalization ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were precipitated out using ProteoExtract precipitation kit (Calbiochem) and dissolved in 1X Laemmli buffer ...
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bioRxiv - Bioengineering 2020Quote: ... Tested additives were added to the qRT-PCR reaction mix at the following concentrations: 1% w/v Tween-20 (Calbiochem 655204-100mL), 0.8 µg/µL BSA (Fisher BP675-1) ...
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bioRxiv - Immunology 2021Quote: ... Proteins were precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem) and quantified by Coomassie (Bradford ...
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bioRxiv - Neuroscience 2021Quote: Calcineurin activity was assessed using Calcineurin activity assay kit (#20700, Calbiochem). Hippocampus tissue of 8 weeks old animals was homogenised in the lysis buffer (25 mM Tris-HCl pH 7.5 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... we used the Superoxide Dismutase Assay Kit II (catalogue no. 574601, Calbiochem, supplied by Merck Pty Ltd. ...
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bioRxiv - Systems Biology 2021Quote: ... The proteins were then precipitated using Proteoextract protein precipitation kit (Calbiochem; cat # 539180) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... the cytoskeleton) were extracted using the “ProteoExtract Subcellular Proteome Extraction kit” (Calbiochem/ MERCK) according to the supplier’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: We used a non-specific Protease Activity Assay Kit (Calbiochem, cat. no. 539125) for 96-well plates as previously described (Schulte et al. ...
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bioRxiv - Developmental Biology 2021Quote: ... All primary/secondary antibodies were diluted in SignalBoost™ Immunoreaction Enhancer Kit (407207–1KIT, Calbiochem). After three washes in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... all fractions were individually precipitated using the Proteoextract Protein Precipitation Kit (EMD Milipore, Calbiochem, 539180-1KIT). For each 600 µL fraction ...
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bioRxiv - Developmental Biology 2021Quote: Native membrane proteins were extracted from transfected cells using the ProteoExtract® Native Membrane Protein Extraction Kit (Calbiochem). Proteins from whole cells and from the membrane and soluble fractions were separated on a 12% SDS-PAGE protein gel ...
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bioRxiv - Neuroscience 2021Quote: Cytoplasmic and nuclear fractions were prepared from cell lysates using a ProteoExtract® Subcellular Proteome Extraction Kit (Calbiochem) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: ... an aliquot of the cushion supernatant and eluate were precipitated using the ProteoExtract protein precipitation kit (Calbiochem 539180) per manufacturer protocol and redissolved in 2X Laemmli buffer (Fisher 50-196-784) ...
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bioRxiv - Neuroscience 2022Quote: Cultures were rinsed twice with PBS and cell membrane extracts were collected according to the method of ProteoExtract Kit (Calbiochem). Samples were heated with equal volumes of SDS sample buffer (Novex ...
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bioRxiv - Physiology 2021Quote: Sample preparation - In-gel digests were performed for each excised sample using the ProteoExtract All-in-One Trypsin Digestion Kit (Cat. No. 650212; Calbiochem, EMD Millipore, Billerica, MA) according to manufacturer’s instructions ...