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Citations for Leica :
551 - 600 of 1613 citations for 2 Chloro 1 1 trifluoromethyl 1 3 4 9 tetrahydro 2H beta carbolin 2 yl ethan 1 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... The experimental setup consisted of (1) our microfluidic device installed on a Leica inverted microscope (DMIL LED Inverted Routine Fluorescence Microscope, Leica, Germany) and imaged using a colour camera (MC170 HD ...
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bioRxiv - Plant Biology 2021Quote: ... tumefaciens clones carrying the appropriate BiFC plasmids using a 1 mL needleless syringe and imaged two days post-infiltration with a Leica TCS SMD confocal microscope (Leica Microsystems) using the preset settings for YFP (Ex ...
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bioRxiv - Cell Biology 2020Quote: ... Interaction between swiprosin-1 and Rab21 in cells was detected using confocal microscopy (Leica TCS SP5, 63×1.4 Apo oil objective) and the number of PLA spots per 1000 µm3 was quantified using IMARIS software.
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bioRxiv - Plant Biology 2022Quote: ... seven-day-old Arabidopsis seedlings were incubated in 150 mM NaCl or 50 μM DNP for 1 hour and were then rapidly frozen with an EM ICE high-pressure freezer (Leica Microsystems).
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bioRxiv - Developmental Biology 2022Quote: ... A second planchet was placed over the first and then the sandwich was immediately frozen in a Leica EMPact 1 High Pressure Freezer (Leica Microsystems). The two halves of the planchet were then separated under liquid nitrogen to expose the tissue within the cavity ...
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bioRxiv - Developmental Biology 2022Quote: ... The fixed tails were placed in 1 × PBS solution for 2 hours and then embedded in 7% agarose gel (1 × PBS as the medium) and cut into 180 μm horizontal sections by a vibratome (LEICA VT1000S). All lizards were released at the exact location of capture in less than 4 hours.
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bioRxiv - Neuroscience 2020Quote: ... The signals of Dicer 1 and B2 Mm2 RNA and images of cells were obtained with a fluorescent microscope(DMi8, Leica Biosystems). The following probes were used:B2 Mm2 RNA probe 2 ...
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bioRxiv - Physiology 2021Quote: ... 20 larvae per tank were anaesthetized with MS-222 (50 mg l-1, Pharma Q) prior to feeding and directly photographed individually with a microscope (Leica M165C). The larvae were then frozen in liquid nitrogen and stored at −80°C until dry mass (DM ...
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bioRxiv - Neuroscience 2021Quote: ... 4 μm paraffin sections were prepared and processed on a Leica BOND Rx automated stainer using citrate-buffer based heat-induced antigen retrieval solution (Epitope Retrieval Solution 1 Bond, Leica, Germany) and DAB-based detection (Leica ...
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bioRxiv - Cell Biology 2022Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... elegans behaviors were recorded at 20 frames/s through a macroscope with an apochromat objective lens (1 ×) (Z16 APO, Leica, Germany) and a CCD camera (1940 × 1460 pixels ...
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bioRxiv - Developmental Biology 2021Quote: ... ten fixed larvae were randomly selected from each culture (Dpf 1-24) and photographed under a microscope (Leica with DFC295 camera). Body length (BL ...
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bioRxiv - Cancer Biology 2020Quote: ... applied at either 1:300 or 1:500 for 15-30 minutes prior to detection and color visualization using Refine DAB Polymer (DS9800, Leica Biosystems). Rabbit primary antibodies were incubated for either 15 or 30 minutes followed by detection with Refine DAB Polymer ...
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bioRxiv - Microbiology 2020Quote: ... were positioned on preheated flat agar plates (1% agar in egg water with 0.017% MS-222) and imaged with Fluorescence Stereo Microscope (Leica M205 FA). The image acquisition settings were as following ...
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bioRxiv - Cancer Biology 2020Quote: ... The standard protocol was followed apart from the primary antibody incubation which was extended to 1 hr at room temperature and the post primary step was excluded.27 BMI1 antibody was used at a 1:200 dilution and antigen retrieval was performed with E1 (Leica Biosystems) retrieval solution for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The mixture was incubated at room temperature for 5 min and 1 μL of each sample was pipetted onto a glass dish and imaged using THUNDER Imaging Systems (Leica Microsystems).
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bioRxiv - Neuroscience 2023Quote: ... cut into 2 cm x 2 cm x 1 cm (LxWxH) tissue blocks using lancets and sectioned into 80 µm coronal sections on a microtome (Leica SM2010R). Brain sections were stored in cryoprotectant at -20 °C and washed in PBS at 4 °C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... they were kept at 28±1°C in dark for 16 h and observed under confocal microscope (Leica microsystems, TCS, SP5). BiFC for each combination was repeated twice and appropriate negative controls were used (Kudla and Bock ...
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bioRxiv - Cancer Biology 2023Quote: ... Primary antibody used for Dp427 was the anti-human Dystrophin form Leica Biosystems (1:25, Dy4/6D3, NCL-DYS1, RRID:AB_442080, Leica Biosystems, Wetzlar, Germany) and the Dystrophin antibody from Abcam (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... The gelatin cube was sectioned coronally with 100 μm thickness (except for sagittal sections tilted 12 degrees away from the midline in Fig. 1) using a vibratome (Leica VT1200). Imaging was performed using upright confocal laser-scanning microscope (LSM880 ...
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bioRxiv - Pathology 2023Quote: ... (Supplementary table 1) were cut into 10 μm-thick sections and mounted on Leica PET-membrane slides (76463-320, Leica Microsystems). Postnatal lung tissues were considered as “healthy” after microscopic/histological analysis by a pulmonary pathologist ...
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bioRxiv - Bioengineering 2023Quote: ... one ending of the tube was connected to the tip of a syringe (cut from a 1 ml syringe; Braun, 9166017V) which held the perfusion needle (Leica, 39471024), and the other ending was immersed in the solution chamber where the animal was placed ...
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bioRxiv - Cancer Biology 2022Quote: ... was incubated at 100 degrees Celsius for 20 minutes (for anti-Cytokeratin 5, Bond Epitope Retrieval 1, pH6.0 (Leica Biosystems, AR9961) was used instead) ...
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bioRxiv - Plant Biology 2023Quote: ... 1-Butanol was gradually replaced with melted paraffin wax (Surgipath Paraplast X-tra Tissue Infiltration/Embedding Media; Leica Biosystems, Wetzlar, Germany) using 1-butanol:paraffin (3:1) ...
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bioRxiv - Biochemistry 2023Quote: ... Rabbit mAb (Mouse Preferred; IHC Formulated) (CST, #12202)) was used 1:200 and developed with Bond Polymer Refine Detection (Leica, DS9800).
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bioRxiv - Plant Biology 2023Quote: ... The transformed onion epidermal cells were inoculated on 1/2MS agar plates for ∼12h in the dark and then visualized under a confocal microscope (Leica SP8) for YFP signals ...
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bioRxiv - Cancer Biology 2023Quote: ... the RNA unmasking conditions for the FFPE tissue consisted of 10-minute incubation with Proteinase K from the Bond Enzyme Pretreatment Kit at 1:1000 dilution (Leica Biosystems). HERV-K (Cat# DVF1-19321) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained with 1 µM siR-DNA solution and subsequently migratory behavior of the cells was recorded under a confocal-microscope (Leica SP8) for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 um (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Sections were mounted on gelatin-coated slides and coverslipped with VECTASHIELD containing DAPI (catalog no ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen and coronally sectioned at a thickness of 25 mm (1:5 series) using a LeicaCM1900 cryostat (Leica Microsystems). Following immunohistochemistry for cFos ...
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bioRxiv - Cell Biology 2024Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Developmental Biology 2024Quote: ... The individuals were first anesthetized (with MS-222, at a concentration of 150 mgL−1) and then photographed under a stereoscopic microscope (Leica, Germany) to measure the TL ...
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bioRxiv - Cell Biology 2024Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Microbiology 2024Quote: ... tissue sections were incubated with the primary antibody (rabbit polyclonal anti-Influenza A virus NP [ThermoFisher Scientific, PA5-32242] diluted 1:500 in Primary Antibody diluent [Leica Biosystems]) for 30 min at ambient temperature followed by a polymer-labeled goat anti-rabbit IgG coupled with alkaline phosphatase (30 min) ...
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bioRxiv - Microbiology 2024Quote: ... the cells were stained with Hoechst (50 µg/mL) and propidium iodide (1 mg/mL) and imaged using M205FCA stereoscope (Leica microsystems).
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bioRxiv - Pathology 2024Quote: ... applied at a dilution of 1:50 for 30 minutes at 25 °C in an automated Leica BOND III system (Leica Biosystems). Visualization of the primary antibody binding to tissue section was performed using BOND Polymer Refine Detection (DS9800 ...
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bioRxiv - Microbiology 2024Quote: ... Sections were subjected to heat-induced epitope retrieval with citrate (Bond Epitope Retrieval Solution 1, Leica Biosystems; CD31, Ki-67, NUMA1) or EDTA (Bond Epitope Retrieval Solution 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... 9-14 sections per case were mounted on PEN membrane slides (Leica) and lightly stained with haematoxylin to facilitate microscopic identification of tumor and stroma areas ...
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bioRxiv - Immunology 2022Quote: ... Mouse tissues (at least 2 mm2/organ) were imaged by confocal microscopy (SP8, Leica) using 40x oil objective ...
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bioRxiv - Cell Biology 2021Quote: ... followed by cryo-immobilization in the EMPACT-2 HPF apparatus (Leica Microsystems; Vienna Austria) as described (Largeau & Legouis ...
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bioRxiv - Systems Biology 2021Quote: ... Mice were anesthetized with 2% isoflurane in oxygen using a precision vaporizer (Leica Biosystems). In each group ...
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bioRxiv - Plant Biology 2021Quote: ... and then transferred at −90 °C into an AFS 2 freeze-substitution device (Leica). Grafts were incubated in a cryosubstitution mix containing only uranyl acetate 0.1 % in pure acetone for 30 h (freeze substitution and embedding protocol adapted from Nicolas et al. ...
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bioRxiv - Genetics 2021Quote: ... Stained 2-day-old animals were analysed by a vertical fluorescent microscope (DM2500, Leica). We selected the area between two pharyngeal bulbs to evaluate the number of β-amyloid deposits ...
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bioRxiv - Developmental Biology 2022Quote: Widefield images were acquired every 2 min with an M205 fluorescent dissection microscope (Leica) equipped with a PLANAPO 2.0x objective ...
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bioRxiv - Cell Biology 2022Quote: ... and 2% FBS) using a Leica EM PACT2 high-pressure freezing unit (Leica Microsystems) with rapid transfer system at high pressure (2100 bar) ...
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bioRxiv - Developmental Biology 2022Quote: ... They were fixed by high pressure freezing with the EMPACT-2 system (Leica Microsystems). Cryosubstitution (FS ...
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bioRxiv - Cell Biology 2024Quote: ... followed by cryo-immobilization in the EMPACT-2 HPF apparatus (Leica Microsystems; Vienna Austria) as described (Largeau & Legouis ...
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bioRxiv - Immunology 2021Quote: ... primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800, Leica Biosystems) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Samples were stained with phalloidin 647 and fluorescent z-stacks were acquired by confocal microscopy (Leica SP8, or Zeiss Examiner Z.1) at a pixel size of 60-70 nm ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were longitudinally cut into slices with a thickness of 1 mm and observed by confocal laser scanning microscopy (CLSM, Leica TCSSP8 MP, Leica microsystems, GER). Semi-quantitative analysis was conducted through the assay of the horizontal and vertical fluorescence intensity of the hybrid layer ...