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Citations for American BioInnovations, :
1 - 48 of 48 citations for 7 CHLORO 3 PHENYL PYRAZOLO 1 5 A PYRIMIDINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... PCSK9 mRNA expression was assessed by real-time PCR using PCSK9 primer sets 5’-TGTCTTTGCCCAGAGCATC-3’ and 5’-GTCACTCTGTATGCTGGTGTC-3 (Integrated DNA Technologies) and SYBR Select Master Mix (ThermoFisher, ABI 4472908).
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Quantitative three-dimensional nondestructive imaging of whole anaerobic ammonium-oxidizing bacteriabioRxiv - Cell Biology 2019Quote: ... The V3-V4 regions of the anammox 16S rRNA gene were amplified with the primer 338F (5’-ACTCCTACGGGAGGCAGCAG-3’) and the primer 806R (5’-GGACTACHVGGGTWTCTAAT-3’) using polymerase chain reaction (PCR) (ABI GeneAmp 9700). The PCR program was as follows ...
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bioRxiv - Genetics 2021Quote: ... the QTR locus in TCERG1 was amplified by PCR using a fluorescently-labelled forward (5’-FAM-AACTGACACCTATGCTTG-3’) and unlabelled reverse (5’-GTTGAAGTGGATACTGCA-3’) primer before sizing by capillary electrophoresis (ABI 3730 genetic analyzer) and Genescan against a LIZ600 ladder of size standards (Thermofisher) ...
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bioRxiv - Immunology 2022Quote: ... The hypervariable region V3-V4 of the bacterial 16S rRNA gene was amplified with primer pairs 338F (5’-ACTCCTACGGGAGGCAGCAG-3’) and 806R (5’-GGACTACHVGGGTWTCTAAT-3’) by an ABI Gene Amp® 9700 PCR thermocycler (ABI, CA, USA). The PCR reaction mixture included 4 μL 5× Fast Pfu buffer ...
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bioRxiv - Neuroscience 2022Quote: ... in QuantStudio 7 Flex (Applied Biosystems, ABI) machine ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR was performed on an QuantStudio 7 instrument (ABI) using 2X Universal SYBR green fast mix (ABClonal) ...
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bioRxiv - Bioengineering 2020Quote: ... The media were collected from the upper channel at different time points (0 h, 1 h and 2 h) and the fluorescence intensity was measured using microplate system (ABI Vii 7).
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA was further diluted by 1/3 and 1/10 in ROX350/HiDi and samples loaded onto capillary electrophoresis sequencers (ABI-3730) on capillary electrophoresis (CE ...
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bioRxiv - Genetics 2022Quote: ... by QuantStudio 3 (ABI). The detailed method was followed by Iijima et al ...
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bioRxiv - Neuroscience 2023Quote: ... and qRT-PCR analysis was run using the ViiaTM 7 Real-Time PCR System (ABI). Primer efficiencies were determined by standard dilution curve analysis ...
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bioRxiv - Genomics 2020Quote: ... the purified DNA was quantified with ABI ViiA 7 and Power SYBR Green Master Mix (ABI 4368706) and normalized by genomic DNA ...
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bioRxiv - Plant Biology 2021Quote: ... The qRT-PCR reaction system (ABI QuantStudio 3) included ...
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bioRxiv - Neuroscience 2022Quote: ... detector system (ABI Quant studio 5) and the SYBR Green detection protocol ...
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bioRxiv - Physiology 2022Quote: ... employing the QuantStudio® 3 Real-Time PCR systems (ABI). qPCR primers are shown in Table S1.
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bioRxiv - Genomics 2020Quote: The purified cDNA or dsDNA samples were assayed by quantitative PCR with ABI ViiA 7 and Power SYBR Green Master Mix (ABI 4368706) using custom designed primers (Additional file 3 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... using a QuantStudio™ 7 Flex device and results were analyzed with QuantStudio™ Real-Time PCR software (both ABI, Waltham, MA, USA)
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bioRxiv - Molecular Biology 2023Quote: ... The reaction was conducted on Applied Biosystems® QuantStudio 3 (ABI, USA) following the conditions ...
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bioRxiv - Cancer Biology 2022Quote: ... and real-time quantitative PCR (QuantStudio ™ 3 Real-Time PCR System, ABI, USA).
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bioRxiv - Microbiology 2020Quote: ... followed by 5 min on ice and then analyzed by ABI Prism 3730XL DNA analyzer (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: qPCRs were carried out in a QuantStudio 5 System (ABI/Life Technologies) equipped with the QuantStudio TM Design and Analysis Software version 1.4.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... We conducted qPCR on 384-well plates (ABI Quantstudio 5, Foster City, CA). For each sample ...
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bioRxiv - Immunology 2021Quote: ... and levels of IFNG transcript are quantified by real-time qPCR (ABI QuantStudio 5) using the TaqMan Fast Advanced MasterMix (ABI ...
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bioRxiv - Molecular Biology 2022Quote: ... RT–qPCR of ame-miR-34 was conducted on a QuantStudio 3 fluorescent quantitative PCR system (ABI Company, USA). AmU6 (GenBank ID ...
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bioRxiv - Biochemistry 2023Quote: ... before the cells were heated at 53°C for 3 min in a Veriti 96-well Thermal Cycler (ABI). Samples were subsequently rested at RT for 3 min ...
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bioRxiv - Microbiology 2022Quote: ... using a 5 μL injector loop on a Tempo LC MALDI Spotting system (ABI-MDS/Sciex). Separation was over a 50 min solvent gradient from 2% ACN and 0.1% TFA (v/v ...
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bioRxiv - Molecular Biology 2019Quote: ... The cycling conditions consisted of denaturation at 95°C for 3 min followed by 40 cycles of 95°C for 3 s and 60 or 63°C for 30 s (ABI 7500 instrument). At the end of the PCR amplification ...
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bioRxiv - Physiology 2024Quote: ... PCR reactions were performed in a 96-well format on an ABI QuantStudio 3 using Fast SYBR Green Master Mix (ABI, cat. 4385612). β2-macroglobulin was used for normalization ...
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bioRxiv - Pathology 2022Quote: ... Real-time PCR was carried out in an Applied Biosystems QuantStudio 5 Real-Time PCR System (ABI, Warrington, UK) with SYBR Green PCR master mix (Takara ...
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bioRxiv - Microbiology 2021Quote: ... Gene-specific products were normalized to a group of 5 housekeeping genes (ACTB, B2M, GAPDH, HPRT1, RPLP0) and quantified using the comparative ΔΔCt method (ABI PRISM 7500 sequence detection system user guide) ...
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bioRxiv - Microbiology 2020Quote: ... were selected for 16S rRNA gene PCR using universal primers (27F 5’-AGAGTTTGATCMTGGCTCAG-3’ and 1492R 5’-TACGGYTACCTTGTTACGACTT-3’) followed by sequencing at First Base Laboratories SdnBhd (Malaysia) using Applied Biosystems highest capacity-based genetic analyzer (ABI PRISM® 377 DNA Sequencer) platforms with the BigDye® Terminator v3.1 cycle sequencing kit chemistry (Masomian et al. ...
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bioRxiv - Plant Biology 2019Quote: ... 25 µL samples were prepared by mixing 5 µL of gDNA sample with 12.5 µL of Sybr Green Mastermix (ABI, Carlsbad, California) along with primers and water ...
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bioRxiv - Developmental Biology 2023Quote: ... Real-time PCR was performed by using a SYBR Premix Ex Taq kit (Q711-02, Vazyme) on the QuantStudio 5 Real-Time System (ABI, USA). The data were normalized against the levels of Rpl7 expression ...
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bioRxiv - Genomics 2021Quote: ... and Silencer Select Negative Control #1 siRNA (ABI) and Nuclease Free Water (ABI ...
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bioRxiv - Molecular Biology 2021Quote: ... and DNA sequences were determined with a 3130×1 DNA Sequencer (ABI). Transfection was performed using Lipofectamine LTX and PLUS Reagent (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... including optimized mouse primers (Supplementary Table 1) on a ViiA7 (Thermofisher/ABI). Cycles consisted of an initial incubation at 50° C and 95° C for 2 and 10 minutes respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was prepared from 1 μg total RNA using the high-capacity reverse transcription kit (ABI) according to the manufactures’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µg total RNA was reverse transcribed using the cDNA archive kit (Applied Biosystems – ABI, USA) following manufacturer’s instructions and the resulting cDNA was used as a template for Real Time PCR analysis ...
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bioRxiv - Microbiology 2019Quote: ... The viral loads in organs were determined using TaqMan fast virus 1-step master mix (ABI, USA). One step RT-PCR was also performed to amplify viral RNA from the organs using MyTaq One-Step RT-PCR kit (Bioline ...
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bioRxiv - Genetics 2020Quote: ... The assay follows the protocol published by the WHO (https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf) and uses the 7500 Fast Dx instrument (ABI) and 7500 SDS software (ABI) ...
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bioRxiv - Microbiology 2019Quote: ... Supernatants were titered by measuring p24gag levels with an HIV-1 p24 antigen capture assay kit (ABI#5447), and also by TZM-bl assay.
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bioRxiv - Neuroscience 2021Quote: ... We performed qPCR using 1 μL of cDNA with 7.60 µl of Taqman Universal PCR Master Mix (with No Amperase UNG, ABI) and 0.75 µl of miRNA-specific PCR TaqMan MicroRNA probes (20X ...
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bioRxiv - Genetics 2019Quote: ... and then 1–2 μg RNA was reverse-transcribed by random hexamer priming and Multi Scribe reverse transcriptase (ABI). Amplification was performed using the primers ...
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bioRxiv - Immunology 2022Quote: ... 95°C for 10 min, 40 cycles of 15 s at 95°C, and 60°C for 1 min; ABI PRISM 7000 Sequence Detection System ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of each replicate was analyzed by nested quantitative PCR (45 cycles of 95°C for 15 seconds and 60°C for 1 minute) using Fast Advanced Master Mix (ABI Life Technologies) in an ABI StepOnePlus Real-Time PCR machine ...
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bioRxiv - Bioengineering 2021Quote: ... The images were obtained with a system developed in-house consisting of two red LED lamps (610-630 nm, GR-PAR38-12W-R-1, ABI, Indianapolis, IN) for excitation ...
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bioRxiv - Microbiology 2023Quote: ... 5 μl of each replicate was analyzed by nested quantitative PCR (45 cycles of 95 C for 15 seconds and 60 ℃ for 1 minute) using Fast Advanced Master Mix (ABI Life Technologies) in an ABI StepOnePlus Real-Time PCR machine ...
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bioRxiv - Genomics 2022Quote: ... was used for RSV subtype detection with TaqPath 1-Step Multiplex Master Mix (No ROX) on Applied Biosystems 7500 Real-Time PCR System (ABI, Life Technologies, Waltham, MA) as instructed ...
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bioRxiv - Genomics 2020Quote: ... sequencing using universal primers (Table 1) followed by sequencing at First Base Laboratories SdnBhd (Malaysia) using Applied Biosystems highest capacity-based genetic analyzer (ABI PRISM® 377 DNA Sequencer) platforms with the BigDye® Terminator v3.1 cycle sequencing kit chemistry [38] ...