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Citations for American BioInnovations, :
1 - 48 of 48 citations for 6 Phenyl 1H imidazo 1 2 b pyrazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Real-time PCR was then performed using a QuantStudio 6 Flex System (ABI) according to the manufacturer’s standard protocol ...
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bioRxiv - Microbiology 2021Quote: ... according to the manufacturer’s instructions and completed on QuantStudio 6 Flex system (ABI). RNA expression levels were determined through the ΔΔCt method and normalized with GAPDH or 18S as a housekeeping gene.
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bioRxiv - Genetics 2019Quote: ... and then 1–2 μg RNA was reverse-transcribed by random hexamer priming and Multi Scribe reverse transcriptase (ABI). Amplification was performed using the primers ...
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bioRxiv - Genetics 2022Quote: ... and the data collection was performed on QuantStudioTM 6 Flex system(ABI, Life, USA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... The media were collected from the upper channel at different time points (0 h, 1 h and 2 h) and the fluorescence intensity was measured using microplate system (ABI Vii 7).
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bioRxiv - Immunology 2019Quote: ... six samples from each run were separately derivatized with TMT 6-plex reagents (ABI, Framingham, MA) according to the instructions provided by the manufacturer ...
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bioRxiv - Plant Biology 2023Quote: ... China) was used for quantitative real-time PCR in a Thermo Fisher system (ABI QuantStudio 6 Flex) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real-time PCR reactions were performed on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR Instrument (ABI). Delta-delta-cycle threshold (ΔΔCT ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... Quantification of gene expression was measured by RT-qPCR on Quant Studio 6 Flex Real -Time PCR system (ABI). Target genes expression was calculated using the ∆∆Ct method and expression was normalized with GAPDH expression levels ...
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bioRxiv - Developmental Biology 2022Quote: ... qPCR was run in 384-well plates on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (ABI) and analyzed in Microsoft Excel.
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bioRxiv - Genetics 2022Quote: ... The F-primer of each microsatellite was labeled with fluorescent dye (6-Fam, Vic, Ned or Pet) and the amplification products were read by ABI 3130xl Fluorescence Reader (Applied Biosystems).
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bioRxiv - Plant Biology 2023Quote: ... by using the HieffTM qPCR SYBR Green Master Mix (Low Rox Plus, cat no. 11202ES08, Yeasen, Shanghai, China) on a QuantStudio 6 Flex PCR (ABI). The qPCR signals were normalized to those of the reference gene PST in pine trees ...
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bioRxiv - Microbiology 2023Quote: ... was performed using SYBR Green Real-time PCR Master Mix (QPK-201, Toyobo) with the QuantStudio 6 Flex multicolor real-time PCR detection system (ABI). Relative mRNA levels were normalized to GAPDH levels and calculated using the 2-ΔΔCT method (Livak & Schmittgen ...
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bioRxiv - Plant Biology 2024Quote: ... with Hieff™ qPCR SYBR Green Master Mix (Low Rox Plus, cat no. 11202ES08, Yeasen, Shanghai, China) on a QuantStudio 6 Flex PCR system (ABI). The qPCR signals were normalized to those of the reference gene PST in Pinus by applying the 2-ΔΔCT method (Fang et al. ...
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bioRxiv - Physiology 2019Quote: ... Quantitative real-time PCR assays were performed under a Real-time PCR Detection System of QuantStudio™6 Flex (ABI, USA) in a 384-well plate format using SYBR Green PCR (TOYOBO ...
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bioRxiv - Biochemistry 2019Quote: ... In some experiments we used 6-FAM-labeled DNA and resolved and quantified products using an Applied Biosystems DNA sequencer (ABI 3130xl). Control experiments using radiolabeled DNA established that the 6-FAM label did not alter the kinetics.
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bioRxiv - Neuroscience 2023Quote: ... was used to perform qPCR using 2× Takara TB Green Mastermix (ABI), and the respective specific primers are shown in Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: Real-time PCR was performed as reported previously (Tan et al., 2019) using the QuantStudio 6 Flex Real-Time PCR System (ABI, Thermo Fisher, Shanghai, China) and Roche LightCycler® 480 (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... Gene expression was conducted using the 2^-deltadeltaCT method using ViiA qPCR system (ABI) following MIQE guidelines [17] ...
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bioRxiv - Immunology 2020Quote: ... the relative standard curve method (see Applied Biosystems User Bulletin #2 https://www.gu.se/digitalAssets/1125/1125331_ABI_Guide_Relative_Quantification_using_realtime_PCR.pdf ...
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bioRxiv - Immunology 2020Quote: ... the relative standard curve method (see Applied Biosystems User Bulletin #2 https://www.gu.se/digitalAssets/1125/1125331_ABI_Guide_Relative_Quantification_using_realtime_PCR.pdf ...
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bioRxiv - Neuroscience 2022Quote: Aliquots (2 μl) of samples were used for MALDI-TOF/TOF MS (ABI 4800 model, Applied Biosystems) measurements ...
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bioRxiv - Neuroscience 2021Quote: ... The protocol followed the manufacturer’s recommendations with the exception of using 2 μl for each RT primer (ABI) in a 10 μl total reaction volume (i.e. ...
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bioRxiv - Immunology 2023Quote: ... 2 μl of cDNA were added to 23 μl of PCR mixture containing 2xSYBR Green Master Mix (ABI) and 0.2 μM of forward and reverse primers ...
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bioRxiv - Neuroscience 2020Quote: ... Each ChIP sample and a range of dilutions of the corresponding input sample (0.01 – 2% input) were quantitatively analyzed with gene-specific primers using the StepOnePlus System (ABI) and SYBR qPCR Powermix (ABI).
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bioRxiv - Neuroscience 2021Quote: ... Each ChIP sample and a range of dilutions of the corresponding input sample (0.01 – 2% input) were quantitatively analyzed with gene-specific primers using the StepOnePlus System (ABI) and SYBR qPCR Powermix (ABI) ...
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bioRxiv - Neuroscience 2020Quote: ... Each ChIP sample and a range of dilutions of the corresponding input sample (0.01 – 2% input) were quantitatively analyzed with gene-specific primers using the 7500 detection system (ABI) and SYBR qPCR Premix (Clontech) ...
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bioRxiv - Neuroscience 2021Quote: ... Synthesized cDNA was used for qPCR by 2× ChamQTM Universal SYBR qPCR Master Mix (Vazyme) on StepOnePlusTM Real-Time PCR System (ABI) or BioRad CFX96 Touch Real-Time PCR system ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was generated from 2 μg RNA in 20 μl reactions using High-Capacity Reverse Transcriptase kit (ABI, Thermo Fisher). Real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Molecular Biology 2019Quote: ... and 2 μl diluted cDNA in Optical 8-Tube Strip using the Applied Biosystems 7300 Real-Time PCR Instrument (ABI, USA). The conditions for real-time PCR were as follows ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were verified by 2% agarose electrophoresis gel and subjected to sequencing by using a 3730XL DNA Analyzer (ABI, USA).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1-10 ng template in 9 μl Nuclease-free water were added to 10.0 μL of 2× TaqMan® Universal PCR master mix (ABI/Life technologies, USA) and 1.0 μL of a 20× combined primers and probes mix (ABI/Life Technologies ...
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bioRxiv - Genomics 2021Quote: ... and Silencer Select Negative Control #1 siRNA (ABI) and Nuclease Free Water (ABI ...
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bioRxiv - Molecular Biology 2021Quote: ... The cDNA was further diluted by 1/3 and 1/10 in ROX350/HiDi and samples loaded onto capillary electrophoresis sequencers (ABI-3730) on capillary electrophoresis (CE ...
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bioRxiv - Molecular Biology 2021Quote: ... and DNA sequences were determined with a 3130×1 DNA Sequencer (ABI). Transfection was performed using Lipofectamine LTX and PLUS Reagent (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... including optimized mouse primers (Supplementary Table 1) on a ViiA7 (Thermofisher/ABI). Cycles consisted of an initial incubation at 50° C and 95° C for 2 and 10 minutes respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... cDNA was prepared from 1 μg total RNA using the high-capacity reverse transcription kit (ABI) according to the manufactures’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 µg total RNA was reverse transcribed using the cDNA archive kit (Applied Biosystems – ABI, USA) following manufacturer’s instructions and the resulting cDNA was used as a template for Real Time PCR analysis ...
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bioRxiv - Microbiology 2019Quote: ... The viral loads in organs were determined using TaqMan fast virus 1-step master mix (ABI, USA). One step RT-PCR was also performed to amplify viral RNA from the organs using MyTaq One-Step RT-PCR kit (Bioline ...
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bioRxiv - Genetics 2020Quote: ... The assay follows the protocol published by the WHO (https://www.who.int/docs/default-source/coronaviruse/protocol-v2-1.pdf) and uses the 7500 Fast Dx instrument (ABI) and 7500 SDS software (ABI) ...
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bioRxiv - Microbiology 2019Quote: ... Supernatants were titered by measuring p24gag levels with an HIV-1 p24 antigen capture assay kit (ABI#5447), and also by TZM-bl assay.
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bioRxiv - Neuroscience 2021Quote: ... We performed qPCR using 1 μL of cDNA with 7.60 µl of Taqman Universal PCR Master Mix (with No Amperase UNG, ABI) and 0.75 µl of miRNA-specific PCR TaqMan MicroRNA probes (20X ...
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bioRxiv - Immunology 2022Quote: ... 95°C for 10 min, 40 cycles of 15 s at 95°C, and 60°C for 1 min; ABI PRISM 7000 Sequence Detection System ...
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bioRxiv - Microbiology 2020Quote: ... 5 μl of each replicate was analyzed by nested quantitative PCR (45 cycles of 95°C for 15 seconds and 60°C for 1 minute) using Fast Advanced Master Mix (ABI Life Technologies) in an ABI StepOnePlus Real-Time PCR machine ...
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bioRxiv - Bioengineering 2021Quote: ... The images were obtained with a system developed in-house consisting of two red LED lamps (610-630 nm, GR-PAR38-12W-R-1, ABI, Indianapolis, IN) for excitation ...
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bioRxiv - Microbiology 2023Quote: ... 5 μl of each replicate was analyzed by nested quantitative PCR (45 cycles of 95 C for 15 seconds and 60 ℃ for 1 minute) using Fast Advanced Master Mix (ABI Life Technologies) in an ABI StepOnePlus Real-Time PCR machine ...
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bioRxiv - Genomics 2022Quote: ... was used for RSV subtype detection with TaqPath 1-Step Multiplex Master Mix (No ROX) on Applied Biosystems 7500 Real-Time PCR System (ABI, Life Technologies, Waltham, MA) as instructed ...
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bioRxiv - Genomics 2020Quote: ... sequencing using universal primers (Table 1) followed by sequencing at First Base Laboratories SdnBhd (Malaysia) using Applied Biosystems highest capacity-based genetic analyzer (ABI PRISM® 377 DNA Sequencer) platforms with the BigDye® Terminator v3.1 cycle sequencing kit chemistry [38] ...