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Citations for ApexBio :
1 - 32 of 32 citations for Tick Borne Encephalitis Virus Envelope Protein Human Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Protein transfer from the Mn2+ phos-tag acrylamide (APExBIO, Houston, USA) gel to the PVDF membrane (Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on a 10% SDS gel containing 20 μM Phos-tag acrylamide (ApexBio) and 100 µM MnCl2 ...
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bioRxiv - Developmental Biology 2019Quote: ... Protein samples were resolved by SDS-PAGE with or without 50 μM phos-tag (ApexBio F4002). OsBZR1 was detected by the commercial anti-OsBZR1 and anti-HSP was used as equal loading control (BPI ...
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bioRxiv - Cell Biology 2020Quote: ... 2 µM Phos-tag Biotin (ApexBio) and 2 ng/ml streptavidin-HRP (Jackson ImmunoResearch)] ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μM Phos-tag chemical (ApexBio) was added to the homemade 6.5% Tris-glycine SDS-PAGE gel bearing a ratio of 149:1 acrylamide/bisacrylamide (10).
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bioRxiv - Genetics 2022Quote: ... Phos-tag chemical purchased from ApexBio was added at the final concentration of 20 μM to the 6.5% SDS-PAGE Tris-Glycine gel with a ratio of 149:1 acrylamide/bisacrylamide (Wang et al ...
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bioRxiv - Plant Biology 2019Quote: ... After adding 50 mM Phos-Tag (APExbio, China) and 100 mM MnCl2 ...
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bioRxiv - Biochemistry 2024Quote: ... pH 6.5 prior to competitive elution with ∼2.5 column volumes of buffer containing 0.3 mg/mL of DYKDDDK tag peptide (A6002, APExBIO). The eluted protein was concentrated on a 10 kDa MWCO PES membrane Vivaspin 6 Centrifugal Concentrator (Sartoris ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-importin α purified from mitotic and interphase HeLa cells was eluted from the beads using 300 µg/ml 3xFLAG-tag peptide (Apexbio). The 58 kDa bands were cut from the gel and processed by the Proteomics Core Facility at UT Southwestern.
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bioRxiv - Biophysics 2020Quote: ... The GABAARs were eluted with aliquots of 200 μg/ml FLAG tag peptide and 100 μg/ml 3X FLAG (ApexBio) in the washing buffer ...
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bioRxiv - Plant Biology 2023Quote: ... The purified protein was digested with PreScission Protease (APEXBIO) to remove GST tag and treated with 25 mM DTT or 50 μM H2O2 at 25 °C for 30 min ...
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bioRxiv - Genomics 2019Quote: ... bound proteins were eluted with 3xFLAG peptide (0.5 mg/ml, ApexBio). Elution was performed 2 times with 0.1 ml 3xFLAG peptide for 20 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Protein samples were resolved by SDS-PAGE and stained with InstaBlue (APExBIO). The intensity of gel bands was quantified with ImageJ (38 ...
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bioRxiv - Cell Biology 2022Quote: ... GST-Lamin and GST-dKeap1 proteins were treated with PreScission Protease (APExBIO). GST pull downs were conducted using Glutathione Sepharose 4B beads (Cytiva 17075601 ...
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bioRxiv - Cancer Biology 2019Quote: ... the bound proteins were eluted with 3xflag peptide (ApexBio, Houston, TX, USA) for 1 h at 4°C.
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bioRxiv - Immunology 2021Quote: ... The protein was eluted with 100 μg/ml 3xFLAG peptide (ApexBio, Cat. no: A6001), incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface protein labeling was accomplished using sulfo-NHS-biotin (APExBio, no disulfide bond) as described above after which crude membrane fractions were isolated as described for the adherent cells ...
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bioRxiv - Bioengineering 2023Quote: ... we used an mRNA encoding enhanced green fluorescent protein (eGFP) with ARCA cap modifications (Apexbio Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... FLAG proteins were eluted for 10-20 mins with 250 ug/ml 3× FLAG peptide (APExBIO) in Isotonic Wash Buffer at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with buffer A supplemented with 0.2 mg/ml 3× FLAG peptide (APExBio A6001), concentrated in a 30-kD MWCO Amicon centrifugal filtration device ...
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bioRxiv - Biochemistry 2022Quote: ... and the proteins were eluted by incubating the beads with 200 μg/mL 3xFLAG peptide (APExBIO, A6001) for 2 hours at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by incubation with buffer A containing 0.5 mg/ml 3xFLAG peptide (ApexBio, Houston TX) at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was eluted with buffer A supplemented with 0.25 mg/ml 3× FLAG peptide (A6001; APExBio) for biochemical assays ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... we incubated the monoclonal Dynabeads MyOne Streptavidin T1 (Thermo) was incubated with purified protein diluent and biotin (APExBIO) or biotin-poly (I:C ...
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bioRxiv - Biophysics 2024Quote: ... The protein was eluted with 5 CV of purification buffer supplemented with 150 μg/mL 3X-FLAG peptide (Apexbio). Fractions containing NavPas were pooled and concentrated ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were eluted using 2 50 μL washes of PBS supplemented with 250 ng/μL 3 × FLAG peptide (ApexBio A6001). CuAAC was performed to append rhodamine-azide onto alkyne probe-labeled proteins and after addition of loading buffer samples were separated on precast 4-20% TGX gels (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: Cells were washed with PBS and pre-treated with 5 µmol L-1 inhibitor of apoptosis protein antagonist BV6 (APExBIO) and 20 µmol L-1 pan-caspase-inhibitor zVAD-fmk (Bachem ...
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bioRxiv - Cancer Biology 2019Quote: ... and rotated at 4° C for 2 h before washing 3 × with 500 μL PBS and subsequent elution of FLAG-tagged proteins with 100 μL of 250 ng/μL 3 × FLAG peptide (APExBIO) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Culture media was washed from cells twice with 1x PBS and then cell surface exposed proteins were labeled with sulfo-NHS-SS-biotin (APExBio) at a final concentration of 1 mg/mL in 1x PBS at 4℃ for 20 minutes ...
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Identification of a PRC2 accessory subunit required for subtelomeric H3K27 methylation in NeurosporabioRxiv - Molecular Biology 2020Quote: ... Protein was eluted twice from the resin by incubating with 300 µL of 500 µg/mL 3X Flag Peptide (APExBIO, A6001) rotating at 4 °C for 20 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... were performed before proteins were eluted using 50 μL of TBS supplemented with 250 ng/μL 3 × FLAG peptide (ApexBio A6001). Supernatant (30 μL ...
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bioRxiv - Immunology 2021Quote: ... The beads were washed with 50 column volumes (CV) of the same buffer and the protein was eluted with 100 μg/ml 3xFLAG peptide (ApexBio, Cat. no: A6001). The eluted protein was incubated with 0.5 mM ADP and 0.5 mM MCC950 (Millipore Sigma ...