Labshake search
Citations for ApexBio :
1 - 32 of 32 citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Protein transfer from the Mn2+ phos-tag acrylamide (APExBIO, Houston, USA) gel to the PVDF membrane (Millipore ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were separated on a 10% SDS gel containing 20 μM Phos-tag acrylamide (ApexBio) and 100 µM MnCl2 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Protein samples were resolved by SDS-PAGE with or without 50 μM phos-tag (ApexBio F4002). OsBZR1 was detected by the commercial anti-OsBZR1 and anti-HSP was used as equal loading control (BPI ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 µM Phos-tag Biotin (ApexBio) and 2 ng/ml streptavidin-HRP (Jackson ImmunoResearch)] ...
-
bioRxiv - Molecular Biology 2021Quote: ... 20 μM Phos-tag chemical (ApexBio) was added to the homemade 6.5% Tris-glycine SDS-PAGE gel bearing a ratio of 149:1 acrylamide/bisacrylamide (10).
-
bioRxiv - Genetics 2022Quote: ... Phos-tag chemical purchased from ApexBio was added at the final concentration of 20 μM to the 6.5% SDS-PAGE Tris-Glycine gel with a ratio of 149:1 acrylamide/bisacrylamide (Wang et al ...
-
bioRxiv - Plant Biology 2019Quote: ... After adding 50 mM Phos-Tag (APExbio, China) and 100 mM MnCl2 ...
-
bioRxiv - Biochemistry 2024Quote: ... pH 6.5 prior to competitive elution with ∼2.5 column volumes of buffer containing 0.3 mg/mL of DYKDDDK tag peptide (A6002, APExBIO). The eluted protein was concentrated on a 10 kDa MWCO PES membrane Vivaspin 6 Centrifugal Concentrator (Sartoris ...
-
bioRxiv - Cell Biology 2020Quote: ... FLAG-importin α purified from mitotic and interphase HeLa cells was eluted from the beads using 300 µg/ml 3xFLAG-tag peptide (Apexbio). The 58 kDa bands were cut from the gel and processed by the Proteomics Core Facility at UT Southwestern.
-
bioRxiv - Biophysics 2020Quote: ... The GABAARs were eluted with aliquots of 200 μg/ml FLAG tag peptide and 100 μg/ml 3X FLAG (ApexBio) in the washing buffer ...
-
bioRxiv - Plant Biology 2023Quote: ... The purified protein was digested with PreScission Protease (APEXBIO) to remove GST tag and treated with 25 mM DTT or 50 μM H2O2 at 25 °C for 30 min ...
-
bioRxiv - Genomics 2019Quote: ... bound proteins were eluted with 3xFLAG peptide (0.5 mg/ml, ApexBio). Elution was performed 2 times with 0.1 ml 3xFLAG peptide for 20 min at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... Protein samples were resolved by SDS-PAGE and stained with InstaBlue (APExBIO). The intensity of gel bands was quantified with ImageJ (38 ...
-
bioRxiv - Cell Biology 2022Quote: ... GST-Lamin and GST-dKeap1 proteins were treated with PreScission Protease (APExBIO). GST pull downs were conducted using Glutathione Sepharose 4B beads (Cytiva 17075601 ...
-
bioRxiv - Cancer Biology 2019Quote: ... the bound proteins were eluted with 3xflag peptide (ApexBio, Houston, TX, USA) for 1 h at 4°C.
-
bioRxiv - Immunology 2021Quote: ... The protein was eluted with 100 μg/ml 3xFLAG peptide (ApexBio, Cat. no: A6001), incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell surface protein labeling was accomplished using sulfo-NHS-biotin (APExBio, no disulfide bond) as described above after which crude membrane fractions were isolated as described for the adherent cells ...
-
bioRxiv - Bioengineering 2023Quote: ... we used an mRNA encoding enhanced green fluorescent protein (eGFP) with ARCA cap modifications (Apexbio Technologies ...
-
bioRxiv - Molecular Biology 2021Quote: ... FLAG proteins were eluted for 10-20 mins with 250 ug/ml 3× FLAG peptide (APExBIO) in Isotonic Wash Buffer at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with buffer A supplemented with 0.2 mg/ml 3× FLAG peptide (APExBio A6001), concentrated in a 30-kD MWCO Amicon centrifugal filtration device ...
-
bioRxiv - Biochemistry 2022Quote: ... and the proteins were eluted by incubating the beads with 200 μg/mL 3xFLAG peptide (APExBIO, A6001) for 2 hours at 4 °C ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by incubation with buffer A containing 0.5 mg/ml 3xFLAG peptide (ApexBio, Houston TX) at room temperature for 30 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... The protein was eluted with buffer A supplemented with 0.25 mg/ml 3× FLAG peptide (A6001; APExBio) for biochemical assays ...
-
Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... we incubated the monoclonal Dynabeads MyOne Streptavidin T1 (Thermo) was incubated with purified protein diluent and biotin (APExBIO) or biotin-poly (I:C ...
-
bioRxiv - Biophysics 2024Quote: ... The protein was eluted with 5 CV of purification buffer supplemented with 150 μg/mL 3X-FLAG peptide (Apexbio). Fractions containing NavPas were pooled and concentrated ...
-
bioRxiv - Cancer Biology 2019Quote: ... Proteins were eluted using 2 50 μL washes of PBS supplemented with 250 ng/μL 3 × FLAG peptide (ApexBio A6001). CuAAC was performed to append rhodamine-azide onto alkyne probe-labeled proteins and after addition of loading buffer samples were separated on precast 4-20% TGX gels (Bio-Rad Laboratories ...
-
bioRxiv - Biochemistry 2021Quote: Cells were washed with PBS and pre-treated with 5 µmol L-1 inhibitor of apoptosis protein antagonist BV6 (APExBIO) and 20 µmol L-1 pan-caspase-inhibitor zVAD-fmk (Bachem ...
-
bioRxiv - Cancer Biology 2019Quote: ... and rotated at 4° C for 2 h before washing 3 × with 500 μL PBS and subsequent elution of FLAG-tagged proteins with 100 μL of 250 ng/μL 3 × FLAG peptide (APExBIO) in PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... Culture media was washed from cells twice with 1x PBS and then cell surface exposed proteins were labeled with sulfo-NHS-SS-biotin (APExBio) at a final concentration of 1 mg/mL in 1x PBS at 4℃ for 20 minutes ...
-
Identification of a PRC2 accessory subunit required for subtelomeric H3K27 methylation in NeurosporabioRxiv - Molecular Biology 2020Quote: ... Protein was eluted twice from the resin by incubating with 300 µL of 500 µg/mL 3X Flag Peptide (APExBIO, A6001) rotating at 4 °C for 20 minutes ...
-
bioRxiv - Cancer Biology 2019Quote: ... were performed before proteins were eluted using 50 μL of TBS supplemented with 250 ng/μL 3 × FLAG peptide (ApexBio A6001). Supernatant (30 μL ...
-
bioRxiv - Immunology 2021Quote: ... The beads were washed with 50 column volumes (CV) of the same buffer and the protein was eluted with 100 μg/ml 3xFLAG peptide (ApexBio, Cat. no: A6001). The eluted protein was incubated with 0.5 mM ADP and 0.5 mM MCC950 (Millipore Sigma ...