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1 - 8 of 8 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag HEK293 since 2019

• LncRNA Malat1 represses Th17 effector program by maintaining a critical bivalent super-enhancer and promotes intestinal inflammation

  Shengyun Ma, et al., bioRxiv - Immunology 2022

  Quote: ... cells were cross-linked with 2 mM disuccinimidyl glutarate (DSG, ProteoChem) and 1% (vol/vol ...
• The fingering patterns in the epithelial layer control the gap closure rate via curvature-mediated force

  Hyuntae Jeong, et al., bioRxiv - Biophysics 2023

  Quote: ... the polymerized PA gel was treated with sulfosuccinimidyl-6-(4-azido-2-nitrophenylamino) hexanoate (Sulfo-SANPAH; Proteochem) 1 mg/ml in 50 mM HEPES buffer (Life Technologies ...
• Directed biomechanical compressive forces enhance fusion efficiency in model placental trophoblast cultures

  Prabu Karthick Parameshwar, et al., bioRxiv - Biophysics 2024

  Quote: ... the gels were activated twice with 0.1 mg/ml of the photoactivable bifunctional crosslinker N-sulfosuccinimidyl-6-[4’-azido-2’-nitrophenylamino] hexanoate (sulfo-SANPAH, ProteoChem); and incubated with an excess amount of 80-100 μl of bovine Collagen ı (0.1 mg/ml in phosphate buffered saline or PBS ...
• Epithelial layer unjamming shifts energy metabolism toward glycolysis

  Stephen J. DeCamp, et al., bioRxiv - Biophysics 2020

  Quote: Polymerized polyacrylamide gels were first treated with 440 μL of a 1:50 (v/v) mixture of Sulfosuccinimidyl 6-(4'-azido-2'-nitrophenylamino)hexanoate (Sulfo-SANPAH) (ProteoChem) and 50 mM HEPES solution under a UV light for 10 minutes ...
• A Machine Learning Approach to Predict Cellular Mechanical Stresses in Response to Chemical Perturbation

  V.A. SubramanianBalachandar, et al., bioRxiv - Biophysics 2023

  Quote: The petri dish samples with PDMS micropatterns stamped on PA gels were then subject to treatment with sulfosuccinimidyl-6-(4-azido-2-nitrophenylamino) hexanoate (Sulfo-SANPAH; Proteochem) dissolved in 0.1 M HEPES buffer solution (Fisher Scientific ...
• Mechanisms of Epigenomic and Functional Convergence Between Glucocorticoid- and IL4-Driven Macrophage Programming

  Dinesh K Deochand, et al., bioRxiv - Immunology 2024

  Quote: Macrophages were crosslinked with 1% methanol-free formaldehyde (Thermo) for 10 min (H3K27ac) or double-crosslinked with 2 mM disuccinimidyl glutamate (Proteochem, c1104) for 30 min followed by 1% methanol-free formaldehyde for 10 min at RT (GR ...
• Time-of-day defines the efficacy of NAD⁺ to treat diet-induced metabolic disease by adjusting oscillations of the hepatic circadian clock

  Q Escalante-Covarrubias, et al., bioRxiv - Physiology 2022

  Quote: ... Dual crosslinking was performed in a final volume of 1ml using 2 mM of DSG (Disuccinimidyl glutarate, ProteoChem, CAS: 79642-50-5) for 10 min at RT on a rotary shaker ...
• High-resolution assessment of multidimensional cellular mechanics using label-free refractive-index traction force microscopy

  Moosung Lee, et al., bioRxiv - Biophysics 2023

  Quote: ... the polymerized PA gel was treated with 1 mg/mL sulfosuccinimidyl-6-(4-azido-2-nitrophenylamino) hexanoate (Sulfo-SANPAH; Proteochem, UT, USA) in 50 mM HEPES buffer (Life Technologies ...