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Citations for Repligen :
1 - 45 of 45 citations for U3 Small Nucleolar RNA Associated Protein 4 Homolog CIRH1A Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Antibody-bound nucleosomes were captured for 1 hr at 4°C using protein A agarose (Repligen) beads ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound nucleosomes were captured using protein A agarose (Repligen) beads ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C followed by Protein A agarose beads (CA-PRI-0100, Repligen) for 1 hr the following morning ...
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bioRxiv - Microbiology 2022Quote: ... antibodies were purified using the Protein A resin (Repligen, CA-PRI-0100). All the antibody preparations were reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Genetics 2020Quote: ... Vectors that encoded the anti-FAM19A5-IgG1 antibody were transfected into HEK293F cells and the recombinant antibody was purified using Protein A beads (RepliGen). Anti-FAM19A5 antibodies that recognized the epitopes formed at N-terminal and C-terminal regions were generated and called N-A5-Ab and C-A5-Ab ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates clarified by centrifugation at 10,000g for 15 minutes at 4°C were incubated with 5ul of serum containing antibodies for 2 hours at 4°C followed by the addition of pre-blocked (with 1% BSA) Protein-A beads (Repligen) for an additional 2 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 mg of anti-MHC-I mouse monoclonal antibodies (W6/32) covalently conjugated to Protein A sepharose (Repligen) using DMP as previously described [42,43] were added to the clarified supernatants and incubated with constant agitation for 2 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates (500 µg for HEK293 or 2 mg for U251N) were incubated with 3 µg of indicated antibodies and 12 µl protein-A sepharose beads (IPA300, Repligen) (co-IP ...
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bioRxiv - Cell Biology 2022Quote: ... 200 µg for HEK293) were incubated with 3 µg of the indicated antibodies and 12 µL protein sepharose beads (IPA300, Repligen) at 4°C for 4 h ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were first purified using CaptivA Protein A affinity resin (Repligen), unless otherwise specified ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein A beads (Repligen) were used to capture antibody-bound protein at 4 °C for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein A beads (Repligen) were blocked in BC150 supplemented with 1% Fish gelatin (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A agarose beads (RepliGen) were blocked for 1 hr at 4°C in Buffer BC150 containing 1% fish skin gelatine (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein A agarose beads (Repligen) were blocked with 1 mg/ml BSA and 1 mg/ml yeast tRNA in 1x TE buffer at 4⁰C for 1 hr ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein A agarose beads (Repligen) were blocked with 1 mg/ml BSA and 1 mg/ml yeast tRNA in native ChIP incubation buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein A agarose beads (Repligen) were used to capture the immunoprecipitated material for 1 h at 4°C ...
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bioRxiv - Physiology 2022Quote: ... and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen). All proteins were further polished with Superdex 200 Increase 10/300 GL (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... The recombinant proteins were purified from supernatants by NGL COVID-19 Spike Protein Affinity Resin (Repligen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Protein A agarose (Repligen, #CA-PRI-0100), Mouse anti-HA magnetic beads (Pierce ...
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bioRxiv - Neuroscience 2022Quote: ... protein-A Agarose beads (Repligen 102500-03) and RFP trap magnetic beads (Chromo Tech rtma ...
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bioRxiv - Immunology 2021Quote: Protein-A sepharose beads (Repligen, Waltham, Mass. USA) were covalently conjugated to 10 mg/mL W6/32 (pan-anti-HLA-I ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein A agarose beads (Repligen, 10-1003-02) or protein A/G magnetic beads (Pierce ...
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bioRxiv - Cell Biology 2022Quote: ... Protein A-Sepharose was from Repligen (Needham, MA). Other common reagents were from Sigma-Aldrich.
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bioRxiv - Cell Biology 2020Quote: ... 60 μL of Protein A agarose bead slurry (Repligen) were washed and resuspended in lysis buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 500 μg of total brain or heart lysate extracts were incubated with 15 μL of Protein-A Sepharose beads (CaptivA protein resin, Repligen, Cat#CA- PRI-0100) and 2 μg of anti-Cav1.2 α1-subunit or control rabbit IgG antibody ...
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bioRxiv - Molecular Biology 2020Quote: Sonicated chromatin was precleared using Protein A agarose beads (Repligen) for 1 hour (1h ...
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bioRxiv - Molecular Biology 2019Quote: ... or protein A agarose beads (Repligen, for histone or RNAPII ChIP) blocked with 1 mg/ml BSA and 1 mg/ml yeast tRNA ...
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bioRxiv - Cell Biology 2022Quote: ... followed by addition of Protein A agarose (Repligen Corporation, Waltham, MA) or protein G agarose (Thermo-Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... MAbs were purified using CaptivA® Protein A Affinity Resin (Repligen), for affinity chromatography ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.4) overnight at 4 C in 3.5 kDa MWCO dialysis tubing (Repligen). 1-1.5 mL of Ni2+ Sepharose Excel (GE Life Sciences ...
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bioRxiv - Neuroscience 2019Quote: ... Immobilized Protein A beads (10μl packed beads/ sample) (Repligen #10-2500-04) were rinsed 3 times with TMT buffer ...
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bioRxiv - Immunology 2020Quote: Preparation of immunoaffinity columns: 1.5 mL of protein A agarose resin (Repligen) was washed (PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... ChIP reactions were incubated with pre-blocked protein A agarose beads (Repligen) for 2 hr (RING1B and SUZ12 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 h incubation with 25 µL Protein A-agarose beads (RepliGen). Beads were washed five times with 0,1 x PBS after which beads were eluted in SDS sample buffer and incubated for 30 min at 37°C.
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bioRxiv - Molecular Biology 2020Quote: ... Diluted chromatin was pre-cleared for 1 hr using Protein A agarose beads (Repligen) that were pre-blocked with 1 mg/ ml BSA and 1 mg/ml yeast tRNA ...
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bioRxiv - Molecular Biology 2019Quote: ... 150 mM NaCl) and pre-cleared for 1 hour using Protein A agarose beads (Repligen) blocked with 1 mg/ml BSA and 1 mg/ml yeast tRNA ...
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bioRxiv - Bioengineering 2022Quote: ... Then the slurry was washed with 10x of 85 mM imidazole in PBS and the active protein was eluted with 250 mM imidazole and dialyzed three times against 100-fold excess of PBS at 4 °C (3,000 kDa molecular weight cut-off, Spectra/Por, Repligen). Finally ...
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bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dialyzed against 3 liters of Milli-Q water at 4°C using a 1 kDa cut-off dialysis tubing (Repligen Spectra/Por 6 Pre-Wetted Regenerated Cellulose ...
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bioRxiv - Synthetic Biology 2021Quote: ... the Avestin EmulsiFlex-C3 homogeniser was used at 15000-20000 psi and the final pellet-free cell extract was dialysed (using a Spectra/Por 4 Dialysis Tubing, 12-14 kD MWCO from Repligen) overnight in S30B buffer before one final centrifugation at 12000xg for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... 1x PIC) and pre-cleared for 1 h with either protein A agarose beads (Repligen, for RING1B ChIP) or protein A magnetic Dynabeads (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... chromatin was diluted to 300 µg/ml with lysis buffer and precleared with Protein A agarose beads (Repligen), blocked with BSA and tRNA ...
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bioRxiv - Molecular Biology 2021Quote: ... The culture supernatant was applied to a column packed with CaptivA® Protein A Affinity Resin (Repligen, Massachusetts, USA) pre-equilibrated with 137 mM NaCl 2.7 mM KCl 10 mM Na2HPO4 2 mM KH2PO4 pH 7.4 (PBS) ...
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bioRxiv - Molecular Biology 2020Quote: ... Each aliquot was rotated for 1 hour with 100 μl 50% Sepharose Protein A Fast-Flow bead slurry (IPA400HC, Repligen) previously equilibrated in Buffer L ...
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bioRxiv - Immunology 2022Quote: ... SmT1v3-R and –D were purified using a one-step affinity method with NGL COVID-19 Spike Protein Affinity Resin (Repligen, Waltham, MA, USA). 150-250 ml of supernatant was applied to a 15-ml column by gravity flow ...
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bioRxiv - Immunology 2023Quote: ... Spike protein from different variants were purified using a one-step affinity method with NGL COVID-19 Spike Protein Affinity Resin (Repligen, Waltham, MA, USA) (19) ...