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Citations for Repligen :
1 - 36 of 36 citations for 5 Pyrimidinecarbonitrile 1 2 3 4 tetrahydro 4 oxo 6 phenyl 2 thioxo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Lysates clarified by centrifugation at 10,000g for 15 minutes at 4°C were incubated with 5ul of serum containing antibodies for 2 hours at 4°C followed by the addition of pre-blocked (with 1% BSA) Protein-A beads (Repligen) for an additional 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dialyzed against 3 liters of Milli-Q water at 4°C using a 1 kDa cut-off dialysis tubing (Repligen Spectra/Por 6 Pre-Wetted Regenerated Cellulose ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody-bound nucleosomes were captured for 1 hr at 4°C using protein A agarose (Repligen) beads ...
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bioRxiv - Bioengineering 2021Quote: An alternating tangential flow filtration system (ATF 2, Repligen) was used for cell retention ...
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bioRxiv - Bioengineering 2021Quote: ... an alternating tangential flow filtration system (ATF 2, Repligen) was utilized ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.4) overnight at 4 C in 3.5 kDa MWCO dialysis tubing (Repligen). 1-1.5 mL of Ni2+ Sepharose Excel (GE Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C followed by Protein A agarose beads (CA-PRI-0100, Repligen) for 1 hr the following morning ...
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bioRxiv - Biochemistry 2023Quote: ... An XCell™ ATF 2 stainless steel device (Repligen, Waltham, MA) with a 0.2 μm hollow filter fiber cartridge (Repligen ...
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bioRxiv - Bioengineering 2022Quote: ... Then the slurry was washed with 10x of 85 mM imidazole in PBS and the active protein was eluted with 250 mM imidazole and dialyzed three times against 100-fold excess of PBS at 4 °C (3,000 kDa molecular weight cut-off, Spectra/Por, Repligen). Finally ...
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bioRxiv - Physiology 2022Quote: ... and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen). All proteins were further polished with Superdex 200 Increase 10/300 GL (GE Healthcare Life Sciences ...
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bioRxiv - Synthetic Biology 2021Quote: ... the Avestin EmulsiFlex-C3 homogeniser was used at 15000-20000 psi and the final pellet-free cell extract was dialysed (using a Spectra/Por 4 Dialysis Tubing, 12-14 kD MWCO from Repligen) overnight in S30B buffer before one final centrifugation at 12000xg for 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 mg of anti-MHC-I mouse monoclonal antibodies (W6/32) covalently conjugated to Protein A sepharose (Repligen) using DMP as previously described [42,43] were added to the clarified supernatants and incubated with constant agitation for 2 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol and 2 mM dithiothreitol (DTT)) in 12-14 kDa Spectra/Por dialysis tubing (Repligen, cat#123697) with mild agitation at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... The PET hydrolase reaction was induced on a cellulose dialysis membrane (Spectra/Por 2 Trial Kit, 12–14 kD: flat width 25 mm; Repligen, USA), and bottle-derived PET powder was used as the substrate ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02% sodium azide) using 6-8 kDa dialysis tubing (Repligen) at 4 °C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Media dialysis was carried out using Spectra/Por®2 dialysis membrane tubing (29mm diameter) with a molecular weight cutoff of 12-14kD (Repligen, Waltham, Ma).
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bioRxiv - Synthetic Biology 2023Quote: ... and buffer exchanged into PBS by overnight dialysis through regenerated cellulose tubing (6-8 kDa MWCO, Repligen). To test conjugation ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02% sodium azide) overnight at 4C using 6-8 kDa molecular weight cut-off dialysis tubing (Repligen) and the expression tag was simultaneously removed using recombinant TEV protease (∼1:20 ratio mg/mg) ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: ... Dialysis was performed against deionized water using Spectra/Por 3 dialysis membrane tubing (Repligen) with a MWCO of 3.5 kDa for 48 hours ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: ... Supernatants from each culture were dialyzed into 1.5 L 1X PBS for 24 hours using 6-8 kDa molecular weight cut off (MWCO) dialysis tubing (Repligen, #132655). Dialysed samples were collected and imidazole was added to a final concentration of 20 mM ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates (500 µg for HEK293 or 2 mg for U251N) were incubated with 3 µg of indicated antibodies and 12 µl protein-A sepharose beads (IPA300, Repligen) (co-IP ...
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bioRxiv - Cell Biology 2022Quote: ... 200 µg for HEK293) were incubated with 3 µg of the indicated antibodies and 12 µL protein sepharose beads (IPA300, Repligen) at 4°C for 4 h ...
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bioRxiv - Bioengineering 2021Quote: ... Alternating tangential flow was set to 0.11 L/min (5 L/m2/min) using an XCell Lab Controller (Repligen). The desired rate of medium exchange was achieved by controlling the flow rate of the permeate (spent medium ...
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bioRxiv - Bioengineering 2021Quote: ... the clarified lysate was concentrated by tangential flow filtration at 5 L/min/m2 using a KR2i KrosFlo (Repligen) with Pellicon 2 mini cassettes (300 kDa ...
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bioRxiv - Cancer Biology 2022Quote: ... The resultant liposomes were then cooled on ice for 5 minutes before dialysis using a 50kDa MWCO regenerated cellulose dialysis bag (Repligen, California, USA) overnight in pH 7.4 HBS at 4°C.
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bioRxiv - Bioengineering 2022Quote: ... The polymer was further purified using dialysis (1 kDa MWCO, Repligen) against 3.5 liters of deionized water with a total of four changes over 72 h at room temperature and lyophilized to obtain a pure white solid powder ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 1 h incubation with 25 µL Protein A-agarose beads (RepliGen). Beads were washed five times with 0,1 x PBS after which beads were eluted in SDS sample buffer and incubated for 30 min at 37°C.
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bioRxiv - Molecular Biology 2020Quote: ... Diluted chromatin was pre-cleared for 1 hr using Protein A agarose beads (Repligen) that were pre-blocked with 1 mg/ ml BSA and 1 mg/ml yeast tRNA ...
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bioRxiv - Bioengineering 2023Quote: ... The product was then purified by dialysis in 1 kg/mol MWCO tubing (Repligen) against deionized water for three days ...
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bioRxiv - Molecular Biology 2019Quote: ... 150 mM NaCl) and pre-cleared for 1 hour using Protein A agarose beads (Repligen) blocked with 1 mg/ml BSA and 1 mg/ml yeast tRNA ...
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bioRxiv - Immunology 2020Quote: ... Formulation was performed by TFF (ProStream mPES,1 m2 10 kDa MWCO, L-screen; Repligen) operated with a TMP of 15 psig at a process flux of 8 LMH ...
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bioRxiv - Biophysics 2024Quote: ... The dissolved peptide was added to a 1 mL Float-A-Lyzer G2 Dialysis Device from Repligen with a 0.5-1 kDa cutoff ...
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bioRxiv - Molecular Biology 2020Quote: ... 1x PIC) and pre-cleared for 1 h with either protein A agarose beads (Repligen, for RING1B ChIP) or protein A magnetic Dynabeads (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: ... provides shear rate (1516 s−1) and other fluid dynamic parameters similar to those of an 11 m2 filter (ATF10, Repligen) used for perfusion of a 2000 L bioreactor of circa 1500 L working volume under recommended conditions ...
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bioRxiv - Bioengineering 2021Quote: ... The submerged addition line was welded to the bioreactor connection line of an XCell ATF 1 single-use hollow-fibre filter and pump device (Repligen) with a 0.22 μm pore size ...
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bioRxiv - Molecular Biology 2020Quote: ... Each aliquot was rotated for 1 hour with 100 μl 50% Sepharose Protein A Fast-Flow bead slurry (IPA400HC, Repligen) previously equilibrated in Buffer L ...