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Citations for Repligen :
1 - 9 of 9 citations for 4 1BBR TNFRSF9 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 200 µg for HEK293) were incubated with 3 µg of the indicated antibodies and 12 µL protein sepharose beads (IPA300, Repligen) at 4°C for 4 h ...
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bioRxiv - Neuroscience 2019Quote: ... CNTNAP2-Fc secreted in the medium was affinity purified using ProteinA-CaptivA TM PriMAB (RepliGen, Waltham, MA) and subsequently cleaved with recombinant 3C protease to remove the Fc fragment and leave the mature (i.e. ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.4) overnight at 4 C in 3.5 kDa MWCO dialysis tubing (Repligen). 1-1.5 mL of Ni2+ Sepharose Excel (GE Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C followed by Protein A agarose beads (CA-PRI-0100, Repligen) for 1 hr the following morning ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody-bound nucleosomes were captured for 1 hr at 4°C using protein A agarose (Repligen) beads ...
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bioRxiv - Bioengineering 2022Quote: ... Then the slurry was washed with 10x of 85 mM imidazole in PBS and the active protein was eluted with 250 mM imidazole and dialyzed three times against 100-fold excess of PBS at 4 °C (3,000 kDa molecular weight cut-off, Spectra/Por, Repligen). Finally ...
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bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dialyzed against 3 liters of Milli-Q water at 4°C using a 1 kDa cut-off dialysis tubing (Repligen Spectra/Por 6 Pre-Wetted Regenerated Cellulose ...
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bioRxiv - Synthetic Biology 2021Quote: ... the Avestin EmulsiFlex-C3 homogeniser was used at 15000-20000 psi and the final pellet-free cell extract was dialysed (using a Spectra/Por 4 Dialysis Tubing, 12-14 kD MWCO from Repligen) overnight in S30B buffer before one final centrifugation at 12000xg for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates clarified by centrifugation at 10,000g for 15 minutes at 4°C were incubated with 5ul of serum containing antibodies for 2 hours at 4°C followed by the addition of pre-blocked (with 1% BSA) Protein-A beads (Repligen) for an additional 2 hours ...