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Citations for ChromoTek :
1 - 44 of 44 citations for 6H Pyrrolo 3 2 e benzothiazole 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... and serine 2 (Chromotek, diluted 1:200). After washing with PBS ...
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bioRxiv - Microbiology 2021Quote: ... The resin was then washed 3 times in GFP-Trap_A Wash buffer (Chromotek) and GFP-proteins were eluted using SDS sample buffer at 95°C for 10 min and analyzed by immunoblotting.
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bioRxiv - Cell Biology 2021Quote: ... Myc-Trap agarose beads A (Chromotek, yta-2 Myc Trap_A) were prepared by 3 rounds of washing in extraction buffer and centrifugation at 1000 x g for 5min at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then transferred in 900 μl aliquots to fresh Eppendorf tubes and incubated with gentle head-over-tail agitation for 2 h at 4°C with 30 μl of anti-RFP or control beads (Chromotek rtma-2 and bmab-20, respectively) that had been washed in 1x lysis buffer.
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bioRxiv - Biochemistry 2019Quote: ... were immunoprecipitated from 2 mg of lysates using GFP-Trap beads (Chromotek) at 4°C for 4h under rotation ...
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bioRxiv - Genetics 2019Quote: ... Immunoprecipitations were performed in the same buffer with 0.5% IGEPAL for 3-4 hours at 4 °C with GFP-trap resin (Chromotek). Beads were washed 3 times with the same buffer and bound proteins were eluted by addition of SDS loading dye and heating to 90°C for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... MEFs were washed extensively and GFP-booster (tagged with Alexa488, ChromoTek, gb2AF488-50, 1:500 in 3%BSA/PBS) and anti-rabbit-Alexa568 (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... 0.25 mM PMSF) and eluted with 4 × 1 mL 0.15 mg/mL (50 µM) 3 x FLAG peptide (Chromotek). The elutant was concentrated with Amico-4 (50k MWCO ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were blocked in 3% BSA in PBS-T for 30 min before incubation with GFP-Booster _Atto488 (1:300; Chromotek), or mouse anti-G3BP1 in 3% BSA in PBS-T for 1 h at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... The immunoprecipitation was performed using 3 mL of plant extract mixed with 25 µL of GFP-trap magnetic beads (Chromotek) at 4 °C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... The extracts were cleared by 10 min centrifugation at 12,000 rpm and the supernatant was incubated on a rotor wheel at 4°C for 3 h with 25 µl of GFP-Trap magnetic agarose bead slurry (Chromotek), pre-washed with extraction buffer ...
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bioRxiv - Biochemistry 2022Quote: ... cleared lysate was incubated with 2 μl washed GFP-Trap magnetic agarose beads (Chromotek) per well for >4 h at 4 °C ...
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bioRxiv - Plant Biology 2019Quote: ... S2Lb-GFP proteins were immunoprecipitated for 2 hours using a GFP-trap system (Chromotek) coupled to magnetic beads ...
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bioRxiv - Microbiology 2021Quote: ... Slides were subsequently blocked overnight in PBS containing 3% BSA before labelling with anti-mNeonGreen mouse monoclonal antibody (32F6; 1:300; Chromotek, Germany) followed by Alexa Fluor 488 goat anti-mouse antibody (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Immunocytochemistry was performed with the following primary antibodies: rat anti-GFP (Chromotek, 50430-2-AP) 1:500 ...
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bioRxiv - Microbiology 2024Quote: ... Lysates from HaCaT cells grown on two 150 mm dishes and infected with HSV-2 strain 186 viruses carrying pUL21-mCherry fusion proteins at an MOI of 3 were prepared at 18 hpi and immobilized on RFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Lysates from 21-GFP cells grown on two 150 mm dishes and infected with HSV-2 strain 186 at an MOI of 3 or uninfected were prepared at 18 hpi and immobilized on GFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... rat antibodies for the detection of phosphorylated RNAPII (serine 5 and serine 2) (Chromotek, 1: 100) and secondary goat anti-rat Alexa Fluor 488 antibodies (A-11006 ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatants were incubated (2 h, at 4°C) with 20 µl GFP-Trap®_MA (ChromoTek). Beads were then washed 3 times for 10 min with 1 ml of IP buffer and eluted with Laemmli sample buffer ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 4 mgs of protein extract per sample was then incubated with GFP-Traps beads (ChromoTek; 3 μl/mg of extract) for 3 hours at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were incubated for 1-2 h at 4°C with GFP-Trap_MA (magnetic beads, ChromoTek, handling according to manufacturer’s manual) ...
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bioRxiv - Plant Biology 2019Quote: ... For RNA Pol II phosphorylated at serine 2 in CTD domain rat 3E10 antibody was used (Chromotek), diluted 1:100 ...
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bioRxiv - Immunology 2023Quote: ... 2 mg of cell lysates were incubated with 25 ml of GFP-TRAP beads (Chromotek, GTA-20) for 1 hr at 4°C on rotation ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were incubated for 2 hours at 4°C with 25 μL GFP-Trap coupled agarose beads (Chromotek). After incubation ...
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bioRxiv - Genomics 2020Quote: ... at 4°C or for 2 hours with 20 uL of GFP-Trap magnetic beads (Chromotek, gtma-20) at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... incubated with a 1:1,000 dilution of mouse anti-GFP [B-2] (SantaCruz Biotechnology) or a rat anti-RFP [5F8] (Chromotek) followed by a 1:5,000 dilution of the horseradish peroxidase-conjugated secondary antibody against mouse IgG (7076 ...
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bioRxiv - Cell Biology 2020Quote: ... For IP 2 mg of cytoplasmic and nuclear extracts were incubated with either 15 µl GFP Trap beads (Chromotek) for 1 h at 4°C or 1-1.5 μg IgG overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Cell Biology 2023Quote: ... Insoluble material was removed by centrifugation at 14,000 x g for 2 minutes and the supernatant was incubated with 50 µl HNG-washed agarose beads (Chromotek) with end-over-end rotation for 15 minutes to capture non-specific binding proteins ...
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bioRxiv - Plant Biology 2020Quote: ... and 2 mM dithiobis [succinimidyl propionate] containing 1x Complete Protease Inhibitor Cocktail) and then incubated with GFP-TRAP agarose beads (ChromoTek) for 2 hours at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated 1 h at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated overnight or 2 nights at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were then incubated for 2 hours at 4°C with 15 μL GFP-Trap coupled to agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were then incubated for 2 hours at 4°C with 150 μL GFP-Trap coupled to agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Cell Biology 2024Quote: ... 0.6 mL of the resulting supernatant (containing 2 – 4 mg of proteins) were supplemented with 15 μL of RFP-Trap magnetic particles (ChromoTek), and incubated for 2.5 h at 4°C on a rotating wheel ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mL of supernatant was incubated at 4°C for 2 h with GFP-Trap_A beads (Chromotek, Planegg-Martinsried, Germany). The beads were collected by centrifugation at 800 × g and washed four times in 1 mL washing buffer (25 mM Tris pH=7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... A volume of 2 mL of total protein was mixed with 25 μL of GFP-TRAP agarose beads (50% slurry, ChromoTek) and incubated shaking overnight at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... The GFP-TRAP agarose beads were centrifuged for 2 min at 3000 x g before washing three times with wash buffer (ChromoTek). Bound proteins were recovered by resuspending the beads in loading buffer (loading dye ...
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bioRxiv - Cell Biology 2022Quote: ... transduced cells were incubated with 100 μL Hybridization Buffer containing 2 μL 12.5 μM CF568-labled gRNA FISH probes and 1:2000 AF647-labeled anti-GFP nanobody (Chromotek, gb2AF647-50) for 4 hours in the dark ...
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bioRxiv - Pathology 2020Quote: ... aliquots of the supernatants equivalent to 2 x 108 cells were incubated with 10 μL anti-Myc agarose beads (Myc-Trap™, Chromotek) for 1 h at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 500μg of lysates in 500 μL of SEC buffer were incubated for 2 hours with GFP-trap magnetic agarose beads (Chromotek #gtma-10) or binding control beads (Chromotek #bmab-20) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with phalloidin diluted in 2% BSA/PBS at room temperature for 20 min or stained with phalloidin and GFP-booster (ChromoTek, gb2AF488-10) when imaging cortactin-EGFP using 3D-SIM ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of the protein lysates was incubated for 2 h with 10 µl of either Myc-Trap® or GFP-Trap® beads (Chromotek). To determine the dependence of interactions on RNA ...
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bioRxiv - Neuroscience 2021Quote: ... glycerol 5% and incubated for 2 h with 30 μl of pre-washed GFP-Trap magnetic agarose beads (gtma-20, ChromoTek, Planegg-Martinsried) using gentle rotation at 4 °C ...
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bioRxiv - Cancer Biology 2020Quote: MDA-MB-231 cells with Tks5αGFP were cultured on HDFC prepared on #1.5 coverglass for 2 days before stained for F-actin (phalloidin-Alexa Fluor-647) and Tks5αGFP (GFP-nanobody, ChromoTek GFP Vhh, # gt-250) using manufacturer recommended procedures ...