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Citations for Invivogen :
1 - 30 of 30 citations for PGK Promoter Lin28 Lentivirus since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... B16-F10 cells expressing luciferase (B16-F10-luc) were generated by infection of recombinant lentivirus stably expressing luciferase luc2 under the control of hEF1A-eIF4G promoter (InvivoGen)61 ...
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bioRxiv - Microbiology 2021Quote: ... or 10 µg/mL blasticidin (InvivoGen; AX526 lentivirus).
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bioRxiv - Genetics 2020Quote: The human GAL5.1 enhancer was cloned into the pCpGfree-promoter-lucia vector (InvivoGen; contains the EF1α promoter) and subjected to CpG methylation using SssI (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... each followed by CpG free hEF1alpha promoter (Invivogen) and SV40 polyA sequences ...
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bioRxiv - Cell Biology 2024Quote: ... Cells infected with pLV-tetO-Tfap2c lentivirus were selected by Zeocinβ (InvivoGen, ant-zn-05) to identify transformants.
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bioRxiv - Genetics 2020Quote: ... namely mEF1 and rEF1 promoters from the pVItro1-msc plasmid (InvivoGen #pvitro1-mcs) and the addition of an attP docking site recognition sequence ...
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bioRxiv - Microbiology 2023Quote: Stable IL-17 promoter reporter cells (HEK-Blue™ IL-17 cells; InvivoGen) were maintained in growth medium consisting of DMEM (Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Microbiology 2021Quote: ... The HCMV enhancer was PCR amplified from the pDrive5 GFP-1 Promoter Test (Invivogen). The E2BS4 and enhancer regions from HPV16 (nt 7451-7857 ...
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bioRxiv - Cancer Biology 2023Quote: ... and A375 cell lines were infected with the lentivirus followed by drug selection using Puromycin (1ug/mL, InVivoGen). An early passage (2 weeks post viral infection ...
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bioRxiv - Immunology 2020Quote: ... We used pNiFty2-IFNB-SEAP and pNiFty2-56K-SEAP promoter-reporter plasmids (InvivoGen; San Diego, CA), encoding the INFβ minimal promoter and the ISG-56K promoter ...
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bioRxiv - Immunology 2021Quote: ... were transduced 3 times with lentivirus containing supernatant and selected with 1 μg/ml Puromycin (Invivogen, San Diego, USA). Surviving cells were expanded and referred to as HEK293 luciferase reporter cells.
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bioRxiv - Genomics 2024Quote: ... THP1 cells expressing ASC-GFP from an NF-κB-dependent promoter were purchased from Invivogen (thp-ascgfp).
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bioRxiv - Cancer Biology 2021Quote: We transduced cells with lentivirus carrying KRAB-dCas9 and sgRNA targeting candidate progrowth enhancers and performed puromycin (2 μg/ml) (InvivoGen) for three days postelectroporation to select against non-transduced cells ...
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bioRxiv - Cell Biology 2024Quote: ... Targeted cells were transduced with the lentivirus and selected in medium containing puromycin (1 μg/mL, InvivoGen, #ant-pr-1) for 2-3 days ...
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bioRxiv - Cancer Biology 2022Quote: B-ALL cells were infected with Cas9-2A-blast lentivirus followed by one-week selection with 5-15 µg/mL blasticidin (InvivoGen, CA). Cells were amplified and tested for Cas9 activity using the reporter assay described in Tzelepis et al.14 Pools with >75% Cas9 activity were used going forward ...
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bioRxiv - Molecular Biology 2021Quote: ... the zebrafish codon-optimized genes of the corresponding NIR FPs and mClover3 were cloned into an expression vector carrying the bidirectional promoter (p-EF1a:2xCMV:EF1a) consisting of two CpG free mCMV enhancers (Invivogen) each followed by CpG free hEF1alpha promoter (Invivogen ...
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bioRxiv - Immunology 2021Quote: Activity of the secreted Lucia luciferase under IRF3 promoter control (THP1 Dual cells) was assessed using QuantiLuc substrate (Invivogen) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... IFN-β promoter activity was induced by transfecting 250 ng of poly I:C (average size 0.2-1 kb) (InvivoGen) during 16 h ...
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bioRxiv - Immunology 2020Quote: The IgJ-GFP construct includes the 1.1kb NdeI/NheI genomic fragment harboring IgJ enhancer activity previously described27 and the 1.4 kb IgJ promoter followed by EGFP gene from pMOD-ZGFPsh vector (Cayla Invivogen), all flanked by two insulators from chicken β-globin gene locus [23] ...
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bioRxiv - Molecular Biology 2020Quote: ... H1299 cells were transduced by the lentivirus vectors and selected with DMEM containing 5 µg/ml puromycin (InvivoGen, San Diego, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... A549-Cas9 cells were transduced with a lentivirus containing hACE2 and subjected to Zeocin (200 μg/ml) (InvivoGen, San Diego, CA, USA) at 72 h after transduction ...
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bioRxiv - Immunology 2021Quote: ... and Rig-I(rawl-korigi) carried a Lucia luciferase reporter construct under control of an IFN-inducible ISG54 promoter (Invivogen) and were cultured in DMEM containing 10% Serum (Fischer Scientific) ...
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bioRxiv - Pathology 2020Quote: ... reporter gene under the control of an IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites (Invivogen).
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bioRxiv - Bioengineering 2024Quote: ... this clone was used to generate a stable pool harboring the human glycosyltransferase gene ST6GAL1 via transfection with a plasmid containing the coding sequence of human beta-galactoside alpha-2,6-sialyltransferase 1 isoform a (hSTGAL1) (GenBank NP_001340845.1) and expressed from a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a derivative of pcDNA3.1(+ ...
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bioRxiv - Bioengineering 2024Quote: ... a clonal 9xKO-hSTGAL1-expressing CHO parent was made to express human A1AT via transfection with a plasmid containing the coding sequence of human alpha-1-antitrypsin (hA1AT) (GenBank NP_000286.3) under a composite promoter mCMV-hEF1-HTLV (InvivoGen, San Diego, CA) in a pcDNA3.1/Zeo(+ ...
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bioRxiv - Immunology 2024Quote: ... HEK-Blue TLR3 and HEK-Blue TLR4 reporter cells expressing SEAP enzyme under NF-kb and AP-1 promoter (Cat. no.# hkb-htlr2, hkb-htlr3 and hkb-htlr4) were purchased from InvivoGen (USA). Human Peripheral blood mononuclear cells (PBMCs ...
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bioRxiv - Immunology 2020Quote: The VRC01 LC-P2A-HC construct was PCR-amplified from engineered C57BL/6J mouse cDNA and cloned between the promoter and constant regions of a mouse IgG2b heavy chain expression vector (InvivoGen, #pfuse-mchg2b) using Gibson Assembly ...
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bioRxiv - Microbiology 2020Quote: ... cells were transduced similarly with supernatant containing ACE2-encoding lentivirus and selected with 1 ug/mL of puromycin (InvivoGen, San Diego, CA, catalogue #ant-pr-1).
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bioRxiv - Cell Biology 2024Quote: ... Isogenic cells stably expressing LEXY from a doxycycline-sensitive promoter were selected by treatment with 200 µg/ml hygromycin B (InvivoGen, ant-hg-5).
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bioRxiv - Immunology 2020Quote: ... Promoter induction was measured by the SEAP reporter secretion (quantified at 620nm following addition of Quanti-Blue™ media, Invivogen, per the manufacturer’s instructions).