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Citations for Epigentek Group :
1 - 22 of 22 citations for Acyl protein thioesterase 2 LYPLA2 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and His-tagged SARS-CoV-2 S1 RBD protein lacking the S1/S2 boundary furin site (EpiGentek) were added at a concentration of 10 ng/well to the Ni-NTA-coated strips and incubated for 1h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... His-tagged peptide containing the SARS-CoV-2-specific furin motif (EpiGentek), and His-tagged SARS-CoV-2 S1 RBD protein lacking the S1/S2 boundary furin site (EpiGentek ...
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bioRxiv - Immunology 2021Quote: ... purified His-tagged ACE2 (EpiGentek) was added at a concentration of 100 ng/well (prepared with PBS ...
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bioRxiv - Immunology 2021Quote: ... A SARS-CoV-2 neutralization antibody (EpiGentek), which targets the spike RBD ...
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bioRxiv - Neuroscience 2020Quote: ... nuclear proteins were extracted using the EpiQuick Nuclear Extraction Kit (Epigentek) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: ... the dCas9 protein was quantified using the CRISPR/Cas9 assay ELISA kit (Epigentek).
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin was isolated from 20 million IHH cells or 40mg of of human fatty liver from donor patients (using the EpiQuik Chromatin Immunoprecipitation Kit (Epigentek) according to manufacturer’s instructions for cells or tissues ...
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bioRxiv - Molecular Biology 2021Quote: EpiQuik Colorimetric General Protein-DNA Binding Assay Kit (Epigentek Inc., cat# P-2004-96) was used for measuring DNA binding ability of PNKP WT ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclear protein was then isolated from cells using the EpiQuik Nuclear Extraction Kit (EpiGentek Group Inc.), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Nuclear proteins were extracted using the EpiQuik Nuclear Extraction Kit following the manufacturer’s instructions (Epigentek, Farmingdale, NY). Nuclear extracts were frozen at − 80°C until use ...
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bioRxiv - Molecular Biology 2023Quote: ... We analyzed SUMOylation of Menin by using the EpiQuikTM In Vivo Universal Protein Sumoylation Assay Kit (EPIGENTEK). Briefly ...
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bioRxiv - Genetics 2021Quote: Extracted DNA (10-200 ng) was bisulphite converted using the Methylamp DNA Modification Kit (#P-1001-2, Epigentek) and eluted in 18 μl elution buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Fourty-eight hours after irradiation histone proteins were isolated using EpiQuik Total Histone Extraxtion Kit (Epigentek, Farmingdale, NY, Cat #OP-0006). Detailed description of the method is available in SI Appendix.
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bioRxiv - Biochemistry 2020Quote: ... with gentle sonication and core histone proteins were extracted with the EpiQuik Total Histone Extraction Kit (Epigentek, Cat# OP-0006-100). The extracted lysates and histone proteins were resolved on a 4-20% gradient gel (Biorad ...
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bioRxiv - Biochemistry 2023Quote: ... The collected nuclear extracts (20 μg protein) were then used to measure global DNA methylation and DNMT and TET activities with kits from Epigentek (USA).
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bioRxiv - Biochemistry 2019Quote: ... Bisulfite conversion of 2 µg DNase treated RNA was performed using the Methylamp One-Step DNA modification kit (Epigentek). The reaction mixture was incubated for three cycles of 90°C for 5 min and 60°C for 1h ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were blocked for 1h in 2% BSA in 0.1% Tween-20 in PBS and then incubated anti-5MC antibody (1:500; A-1014 Epigentek) in 0.1% Tween-20 in PBS overnight at 4°C and subsequently after washing with 1:500 anti-mouse Cy5-conjugated in 0.1% Tween-20 in PBS for 1 h at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... isolated from hypothalamus and PFC of F0 and F1 male mice was diluted in immunoprecipitation buffer and incubated with 2 μg 5-methyl cytosine antibody (A-1014; Epigentek) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Insoluble samples were sonicated at 16°C for 4 minutes on/off with 2-second pulses and 20% amplitude using a water bath sonicator (EpiSonic 2000, EpigenTek), centrifuged at 20,000 g for 30 minutes at 20°C and the supernatant was collected as Triton-insoluble fraction ...
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bioRxiv - Neuroscience 2023Quote: Core histone proteins from E13.5 MGE samples (pooled from 2-4 brains per genotype) were extracted using EpiQuik Total Histone Extraction kit (Epigentek# OP-0006). We obtained 40-80 μg of histone proteins per extraction and loaded 20 μg of protein onto a 4-12% Bis-Tris Plus mini gel (Invitrogen# NW04120BOX) ...
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bioRxiv - Immunology 2023Quote: ... cat# 40591-MM43) combined with an HRPO-labelled detection antibody from the SARS-CoV-2 spike RBD Antibody Pair (Epigentek cat# A73682). Capture antibody was coated at 200 ng/well and detection antibody was used at a dilution of 1:1000 ...
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bioRxiv - Microbiology 2022Quote: Assessment of Gal-9 effects on the binding of the SARS-CoV-2 spike to human ACE2 was performed using a commercially available spike-ACE2 binding assay kit (CoviDrop SARS-CoV-2 Spike-ACE2 Binding Activity/Inhibition Assay Kit, EPIGENTEK, D-1005-48) following the protocol provided by the manufacturer ...