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Citations for Molecular Devices :
1 - 50 of 50 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Hydrated anti-human IgG Fc biosensors (Molecular Devices) were dipped in expression medium containing RBD-IgG1 for 60 s ...
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bioRxiv - Biochemistry 2020Quote: ... IgG1 Fc-fused sACE22 (wild type or engineered variant sACE22.v2.4) were immobilized at 100 nM for 10 minutes to anti-human IgG Fc biosensors (Molecular Devices). The assay buffer was 10 mM HEPES pH 7.6 ...
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bioRxiv - Neuroscience 2022Quote: Whole cell recordings were obtained from HEK293 cells using an Axopatch 200B amplifier (Molecular Devices, San Jose, CA). Recordings were filtered at 5 kHz with the amplifier internal filter and digitized at 50 kHz using a Digidata 1550B digitizer (Molecular Devices ...
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bioRxiv - Biophysics 2023Quote: ... HEK293 cells were recorded at room temperature (25 ± 2 °C) using an Axopatch 200B amplifier (Molecular Devices, USA) with a holding potential of -60 mV ...
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bioRxiv - Microbiology 2020Quote: ... nanoluciferase expression as a surrogate for virus replication was quantitated on a Spectramax (Molecular Devices) plate reader according to the manufacturer’s instructions (Promega ...
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bioRxiv - Microbiology 2022Quote: ... Both HEK293 and Vero E6 toxicity assays luminescence readings were recorded using a SpectraMax® M5 (Molecular Devices®) plate reader.
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bioRxiv - Neuroscience 2023Quote: Whole-cell patch-clamp recordings from mouse and rat primary DRG neurons and transfected HEK293 cells were performed with an Axon patch 700B amplifier/Digidata 1440 and pCLAMP 10.0 software (Molecular Devices) or using EPC10 amplifier and Patchmaster/Fitmaster V2 software (HEKA instruments ...
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bioRxiv - Physiology 2022Quote: ... Cells infected with the virus were imaged inside a BSL3 lab on a PicoXpress microscope (Molecular Devices) on 96 well plates alternatively excited for 500ms through the FITC 445-485/509-539 filter cube and 800ms through the customized F49-395 395/25ET Bandpass ...
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bioRxiv - Neuroscience 2020Quote: Whole-cell recordings of transfected HEK293 cells were performed at room temperature (25 °C) using an Axopatch 200B amplifier (Molecular Devices). Electrodes were pulled with borosilicate glass capillaries by a programmable puller (P-1000 ...
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bioRxiv - Genetics 2020Quote: TALK-1 channel currents were recorded in HEK293 cells using a whole-cell voltage-clamp technique with an Axopatch 200B amplifier and pCLAMP10 software (Molecular Devices), as previously described9 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Whole-cell patch-clamp measurements were performed on transfected HEK293 cells expressing rat version of the GluN1-4a/GluN2A (GluN1/GluN2A) receptors using an Axopatch 200B amplifier (Molecular Devices) as described 25,51 ...
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bioRxiv - Microbiology 2020Quote: ... the OD600nm was measured using a multifunctional microplate reader (Thermo Multiskan FC, Molecular Devices, USA). The assays were conducted in triplicates.
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bioRxiv - Microbiology 2020Quote: ... Fluorescence was measured every 60s for 1 hour after addition of virus on a FilterMax F5 multimode microplate reader (Molecular Devices). Enzyme Vmax and Km was calculated using Prism software (GraphPad).
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bioRxiv - Molecular Biology 2021Quote: ... Stitched images of 9 fields covering the central 50% of the well were analyzed for virus infected cells using Cell Reporter Xpress software (Molecular Devices). The cell number was determined by automated counted of DAPI stained nuclei and infected cells were identified as those cells in which positive N staining was detected associated with nuclear DNA staining.
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bioRxiv - Microbiology 2020Quote: ... Images for quantification of virus infection and cell viability were acquired with a fluorescence microscope and analyzed using ImageXpress Micro XLS (Molecular Devices, Sunnyvale, CA). Images for assessment of tagRFP-TMEM41B subcellular localization were obtained using a Revolve inverted microscope (Echo ...
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bioRxiv - Biophysics 2022Quote: Protein-protein interaction studies are carried out using Fortebio OctateK2 (Molecular Devices, USA). 0.5 mg/ml of PduBB’ or PduCDE (PduCDE in case of enzyme-shell interaction study ...
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bioRxiv - Microbiology 2021Quote: ... the IgGs were captured on anti-human IgG (AHC) biosensors (Molecular Devices). For BLI measurements involving Strep-tagged antigens ...
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bioRxiv - Microbiology 2021Quote: The concentrations of protein samples were predetermined using the Quickdrop from Molecular Devices. The unfolding of our trimeric protein by heat was performed as described in (Chen and King ...
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bioRxiv - Pathology 2019Quote: ... The total protein concentration was determined using a SpectraMax® Plus 384 (Molecular Devices) spectrophotometer with a 562 nm filter using the Pierce™ BCA Protein Assay kit (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were determined via a Bradford assay using SpectraMax Plus 384 (Molecular Devices). Pooled fractions were concentrated via 10 kDa centrifugal filters (Amicon Ultra Ultracel-10K ...
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bioRxiv - Synthetic Biology 2022Quote: ... Protein expression was monitored at 37°C in a SpectraMax i3x plate reader (Molecular Devices). GFP was monitored at ex ...
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bioRxiv - Immunology 2021Quote: ... and protein-of-interest average intensity fluorescence was analyzed using MetaMorph software (Molecular Devices, CA).
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bioRxiv - Microbiology 2023Quote: Protein binding kinetics was analyzed using BLI assays with the Octet RED96 instrument (Molecular Devices) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... We quantified the resulting supernatants for their protein concentrations on a SpectraMax M2 (Molecular Devices) using the DC Protein Assay Kit II (BioRad) ...
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bioRxiv - Bioengineering 2019Quote: ... Protein signal data were extracted from the multicolor fluorescent images using GenePix Pro 6.1 (Molecular Devices) by aligning a microtrough array template with feature blocks per antibody per microtrough to the protein signal features ...
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bioRxiv - Neuroscience 2021Quote: Absorption spectra of enriched protein samples were recorded on a SpectraMax 190 Microplate Reader (Molecular Devices) at 23°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein assay plates were quantified using a SpectraMax M2e plate reader (Molecular Devices, San Jose, CA).
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bioRxiv - Developmental Biology 2021Quote: ... was performed to quantify protein using a SpectraMax M5 microplate reader (Molecular Devices, San Jose, CA, USA). 40 µg of protein was electrophoresed on a 10% SDS polyacrylamide gel as previously described (Smith et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... was performed to quantify protein using a SpectraMax M5 microplate reader (Molecular Devices, San Jose, CA, USA). 40 µg of protein was electrophoresed on a 10% SDS polyacrylamide gel as previously described (Smith et al. ...
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bioRxiv - Synthetic Biology 2023Quote: ... Evaluation of in vivo reporter protein expression was performed using a SpectraMax M2 plate reader (Molecular Devices) to record absorbance (λab = 600 nm ...
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bioRxiv - Synthetic Biology 2019Quote: ... The UV absorbance at 280 nm was mensurated as the protein concentration by SpectraMax M2e(Molecular Devices, American) (Annamalai et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extract was employed for fluorometric quantification of GUS activity using a Spectra Max M2 flouorometer (Molecular Devices) (Monte ...
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bioRxiv - Microbiology 2020Quote: Protein interaction studies via Bio-layer interferometry (BLI) were performed on an Octet K2 instrument (Fortébio Molecular Devices (UK) Limited ...
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bioRxiv - Pathology 2023Quote: ... The concentration of soluble protein was determined using BSA as a standard (SpectraMax iD5, Molecular Devices, Sunnyvale, CA, USA). Equal quantities of protein (30 - 60 μg ...
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bioRxiv - Microbiology 2024Quote: Protein binding kinetics were evaluated through Bio-Layer Interferometry (BLI) assays conducted on the Octet RED96 instrument (Molecular Devices). Briefly ...
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bioRxiv - Neuroscience 2019Quote: ... The linear density and total gray value of each synaptic protein cluster were measured automatically using MetaMorph Software (Molecular Devices). Cultures directly compared were stained simultaneously and imaged with the same acquisition parameters ...
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bioRxiv - Pathology 2019Quote: Protein concentration was determined using the Bio-Rad Protein Assay according to the manufacturer’s protocol with absorbance read at 595 nM on a SpectraMax ID3 spectrophotometer (Molecular Devices). Proteins were denatured in NuPage sample buffer containing 2.5 % β-mercaptoethanol and equal amounts (10-20 µg ...
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Kinetic analysis reveals the rates and mechanisms of protein aggregation in a multicellular organismbioRxiv - Biophysics 2020Quote: Relative protein amounts in developing AM141 animals were determined from fluorescence images acquired on the ImageXpress confocal platform (Molecular Devices). Relative intracellular protein concentrations were obtained by dividing the integrated fluorescence intensity per animal by the total fluorescent volume per animal determined as described above.
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bioRxiv - Neuroscience 2022Quote: The protein concentration of each sample was measured using the Lowry method and a SpectraMax M3 plate reader (Molecular Devices) was used to measure the colorimetric reaction ...
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bioRxiv - Plant Biology 2020Quote: ... Then fluorescence intensity of 100ul filtered medium and 0.1g mycelium proteins was measured with microplate reader (Molecular Devices i3x, Shanghai, China), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration was determined using a Bio-Rad protein concentration assay dye and absorbance read at 595 nm on a spectrophotometer (SpectraMax ID3, Molecular Devices). 20µg total protein in βmercaptoethanol-containing loading buffer was added per well onto 4-12% SDS-PAGE gels (NuPAGE ...
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bioRxiv - Cell Biology 2021Quote: ... Densitometric quantification of protein expression as detected by Western blotting was essentially performed following standard procedures using MetaMorph (Molecular Devices, USA). In brief ...
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bioRxiv - Bioengineering 2019Quote: ... the microchambers were flushed and the captured protein or metabolite on the arrays were converted into fluorescent readout and digitized by a Genepix scanner (Molecular Devices).
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bioRxiv - Systems Biology 2020Quote: ... Measurements for cAMP ELISA and BCA Protein Assay kits were performed in a FlexStation 3 Multi-Mode Microplate Reader (Molecular Devices). The cAMP dose response to light was fit to a hyperbolic equation and confidence intervals were extracted by bootstrapping in a custom Python Script available on GitHub (https://qithub.com/jpfon/cAMP).
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bioRxiv - Genomics 2023Quote: ... to determine the amount of protein necessary to generate signal within the linear range of the GenePix 4300B Microarray Scanner (Molecular Devices) at a resolution of 5µm ...
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bioRxiv - Neuroscience 2020Quote: ... and puncta size and the density of the indicated presynaptic marker proteins were analyzed in a blinded manner using MetaMorph software (Molecular Devices Corp.).
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bioRxiv - Biochemistry 2020Quote: ... absorbance at 280 nm (protein) and 498 nm (vesicles) were determined in a Spextramax® Paradigm® microplate reader (Molecular Devices, CA, 95134, USA). Absorbance at 280 nm and 498 nm were plotted against the qEV-eluted fractions.
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bioRxiv - Evolutionary Biology 2023Quote: ... absorbance for each sample and protein standard was measured at 562 nm in a SpectraMax plate reading spectrophotometer (Molecular Devices, LLC, San Jose, CA). The standard curve was used to convert absorbances to total protein content (μg ind-1).
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bioRxiv - Zoology 2023Quote: ... The carbonyl and protein contents were determined by reading the absorbance at λ⍰=⍰370⍰nm and 280 nm with a SpectraMax 190 spectrophotometer (Molecular Devices, California, USA) for each sample against an appropriate blank ...
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bioRxiv - Microbiology 2023Quote: McpR protein concentration was determined (i) by measuring the absorbance of the sample at 280 nm with the SpectraMax iD3 (Molecular Devices, San Jose, CA, USA) or (ii ...