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Citations for Biotium Inc. :
1 - 46 of 46 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... cells were lysed directly in 1X RIPA buffer and blots were incubated with goat anti-mouse 680 (1:5000, cat #20253, Biotium). Fluorescent immunoreactivity was imaged on a LICOR Odyssey.
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bioRxiv - Neuroscience 2024Quote: ... Blots were blocked with fish gelatin (Biotium, #22010) in TBS for 1 h at RT and then probed with anti-C3 (1:2000 ...
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bioRxiv - Cell Biology 2020Quote: ... Immunostaining was performed by first blocking in permeabilizing blocking buffer and background suppression buffer from Biotium (TrueBlack® IF Background Suppressor System 1:100 dilution in PBS ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... ; Blots were washed 3 times in TBS/0.1% Tween and incubated with secondary IRD-tagged antibodies (CF770, Biotium) diluted 1:10,000 in Odyssey blocking buffer for 1 h at room temperature.
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bioRxiv - Cancer Biology 2024Quote: ... in Tyramide Amplification Buffer Plus (Biotium, 22029) as described by the manufacturer ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were permeabilized using Permeabilization Buffer (Biotium #22016) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Wash Buffer with 1 mM ADVASEP-7 (Biotium; 70029), and finally Wash Buffer alone ...
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bioRxiv - Neuroscience 2022Quote: ... the blocking buffer (1x Fish Gelatin Blocking Agent (22010, BioTium), 0.5% Triton x-100 in PBS ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... blocked for 1 h in TrueBlack® Blocking Buffer (Biotium, 23013), and incubated overnight at 4°C primary with antibodies diluted in TrueBlack® Antibody Dilutent (Biotium ...
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bioRxiv - Cell Biology 2022Quote: Membranes were then blocked in either TrueBlack WB Blocking Buffer (Biotium) or EveryBlot Blocking Buffer (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2019Quote: ... The final three wells were prepared with Luciferase Assay Buffer (Biotium #30085). Plates were scanned in a microplate reader (Biotek Synergy H1 ...
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bioRxiv - Immunology 2020Quote: ... medium was removed and cells were lysed in Passive lysis buffer (Biotium). Luciferin substrate (Xenogen ...
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bioRxiv - Molecular Biology 2022Quote: PCR products were mixed with 1x GelRed prestain loading buffer (Biotium #41010), separated on a 2% or 4% agarose gel (Seakem LE agarose ...
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bioRxiv - Microbiology 2020Quote: ... Gels were post-stained for 10 minutes in 0.5X TB Buffer with GelGreen (Biotium) and rinsed with sterile water before imaging with UV on an EZ Dock Imager (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with primary antibodies diluted in TrueBlack® IF Blocking Buffer (Biotium) for 2 hours at RT ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated with secondary antibodies diluted in TrueBlack® IF Blocking Buffer (Biotium) for 1 hour at RT ...
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bioRxiv - Microbiology 2023Quote: ... The cells were lysed using lysis buffer supplied with Renilla Luciferase Assay Kit 2.0 (Biotium). 20 µl of cell lysate was mixed with 100 µl of freshly prepared working solution and the luminescence was recorded using a TECAN spectrophotometer.
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bioRxiv - Biophysics 2023Quote: ... we fixed the samples using a fixative buffer composed of 4% Paraformaldehyde (PFA, Biotium, Freemont, CA) supplemented with 2.5% of Glutaraldehyde (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: Free Ca2+ calibration solutions were made using Calcium Calibration Buffer Kit #1 (Cat. No. C3008MP, Biotium) according to standard protocol ...
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bioRxiv - Biophysics 2024Quote: ... the cells were incubated in AnV buffer (1:175 dilution of the fluorescently tagged AnV (Biotium) in Hank’s balanced salt solution ...
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bioRxiv - Neuroscience 2020Quote: ... on a 1% Agarose Gel (LifeTech 16500500; 1X TBE buffer, LifeTech 16500500) with GelRed (Biotium 41003-T) for gel electrophoresis ...
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bioRxiv - Microbiology 2020Quote: ... Binding was analyzed by gel electrophoresis on a 7 % polyacrylamide gel in TBE buffer stained with GelRed (Biotium) following electrophoresis.
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bioRxiv - Biochemistry 2022Quote: ... Samples in 25 µl permeabilization buffer were subjected to copper-catalyzed alkyne-azide conjugation to CF405M-azide (Biotium) by addition of 100 µl click reaction mix (50 mM HEPES pH 7.5 ...
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bioRxiv - Genomics 2023Quote: ... rinsed in PBS for 10 min at RT and blocked using blocking buffer (Fish Gelatin blocking agent, BIOTIUM) in the dark for 1 hr ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then washed with annexin-binding buffer and additionally incubated with NucSpot Live 650 (1:1,000; Biotium) for 1 hr at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... The full reaction volume was then loaded onto an 8% acrylamide 0.5x Tris-borate (TB) gel and ran for 60 minutes at 100V and stained with 0.5x TB buffer with GelGreen (Biotium) before imaging using an EZ Dock Imager (Bio-Rad) ...
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bioRxiv - Molecular Biology 2021Quote: ... 50 μg of RNA were biotinylated in biotinylation buffer (10 mM Tris-HCl pH 7.4, 1 mM EDTA, 20 ng/μl MTSEA Biotin-XX (Biotium)) for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... The amplification buffer and enzyme mixes provided in library kit was used for the master mix together with EvaGreen for qPCR (Biotium).
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bioRxiv - Microbiology 2019Quote: ... was incubated in biotinylation buffer (50 mM HEPES [pH 7.5], 5 mM EDTA) and 5 μg MTSEA-biotin (Biotium #90066), rotating at room temperature in the dark for 30 min ...
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bioRxiv - Genetics 2020Quote: ... 6 μl of the PCR product was used for electrophoresis in Tris-Acetate EDTA running buffer at 120V for 45 min on a 2% agarose gel stained with GelRed (Biotium). Experiments were repeated twice to verify the results ...
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bioRxiv - Neuroscience 2020Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594- or CF488A-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated donkey anti-mouse IgG ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were run on a 1.5-2% agarose gel (7-01P02-R, BioConcept) in TAE buffer (3-07F03-I, BioConcept) and imaged using GelRed Nucleic Acid Stain (41003, Biotium). For RT-qPCR ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The slices were then washed three times with the blocking buffer and then incubated with secondary antibodies (CF594-conjugated donkey anti-rabbit IgG, 1:1000, Biotium; CF488A-conjugated goat anti mouse IgG ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were separated on a 1.5-2% agarose gel (7-01P02-R, BioConcept) dissolved in TAE buffer (3-07F03-I, BioConcept) and visualized using GelRed Nucleic Acid Stain (Cat# 41003, Biotium). Presence of rat cells in myoblast culture/muscle lysate was assessed by PCR for rat dystrophin using primers Rat Dmd i22-i23.F/ Rat Dmd i22-i23.R and GoTaq G2 Hot Start Green Master Mix (M7423 ...
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bioRxiv - Immunology 2024Quote: ... The membranes were placed in an opaque gel box and incubated with blocking buffer (PBST + 10% Fish gelatin (PBS 10X, pH 7.2, Thermo Fisher Scientific, Cat-No. 70-013-032; Biotium 10X Fish Gelatin Blocking Agent ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then re-hydrated in PBS for 10 minutes before blocking with TrueBlack® IF blocking buffer (Biotium #23012B) for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Infected cells were then washed with PBS buffer (pH 7.4) and resuspended in complete RPMI containing NucSpot® Live 488 (Biotium, #40081) following the manufacturer recommendations ...
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bioRxiv - Cell Biology 2019Quote: ... The Calbryte-590 loaded cells were then incubated in HBSS buffer containing 0.5 µg/mL CF 488-tagged-Annexin-V (Biotium, cat. 29011), a fluorescence PS probe ...
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bioRxiv - Genomics 2021Quote: ... 100 µL 2x copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction buffer was added to cells to append a fluorophore reporter (2x concentrations: 10 µM CF488A picolyl azide (Biotium #92187), 2 mM CuSO4 (Sigma C8027) ...
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bioRxiv - Plant Biology 2023Quote: ... Amplicons products were analyzed by electrophoresis on a 1% agarose gel in Tris-acetate-EDTA (TAE) buffer stained with GelRed® Nucleic Acid Gel Stain (Biotium) and visualized under UV light.
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bioRxiv - Genomics 2023Quote: ... PCR products were analyzed by electrophoresis on a 1% agarose gel in TAE buffer stained with GelRed® Nucleic Acid Gel Stain (Biotium) and visualized under UV light.
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were washed twice in Wash buffer without TritonX-100 and resuspended in 100 µl of 40% ethanol containing TrueBlack Lipofuscin Autofluorescence Quencher (Biotium, #23007) for 40-50 seconds ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were washed twice in Wash buffer without TritonX-100 and resuspended in 100 µl of 40% ethanol containing TrueBlack Lipofuscin Autofluorescence Quencher (Biotium, #23007) for 40-50 seconds.
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bioRxiv - Cell Biology 2024Quote: ... The fluorescence values of these reads were compared to those read from calcium standards made with the Buffer Kit for Calibration of Fluorescent Ca² Indicators (Biotium, 59100). To measure free magnesium concentrations ...
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bioRxiv - Genetics 2020Quote: ... PCR product was examined by 1.5% (w/v) agarose 0.5x TBE buffer (45mM Tris-borate, 1mM EDTA) gel electrophoresis with GelGreen stain (Biotium Inc., Fremont, CA, USA).
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bioRxiv - Neuroscience 2020Quote: ... samples were passively incubated for 48 hours at room temperature in eFLASH sample buffer.32 Rapid 24 hour immunostaining of whole hemispheres with polyclonal CF640R rabbit anti-RFP (Biotium, Fremont, CA) was carried out using the eFLASH protocol.32 The samples were again passively washed in PBSN three times for a minimum of 12 hours each before refractive index matching.