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Citations for Biotium Inc. :
1 - 47 of 47 citations for Recombinant Human KDR Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells that were imaged were labeled using CF640-conjugated streptavidin to label biotinylated proteins (CF640R Streptavidin, Biotium cat 29041). Affinity purification was performed using streptavidin-conjugated magnetic beads (Pierce ...
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bioRxiv - Cell Biology 2021Quote: ... 70 μg of RNA was biotinylated with MTSEA biotin-XX (Biotium) as previously described (Chan et al. ...
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bioRxiv - Immunology 2021Quote: ... Oxidized vicinal diols were subsequently biotinylated with 1 mM biocytin hydrazide (Biotium) in the presence of 10 mM aniline (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... biotinylated Donkey anti-Sheep IgG (for ChAT, 1:250, #20191-1, Biotium). All antibodies were diluted in the blocking solution.
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bioRxiv - Cell Biology 2021Quote: ... and then fragmented with NaOH and biotinylated with MTSEA biotin-XXlinker (Biotium). The biotinylated RNA was then purified using µMACS Streptavidine MicroBeads (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was biotinylated with 10 µg of MTSEA biotin-XX (Biotium) in 100 mM HEPES/pH 7.5 and 10 mM EDTA for 30 min at 25°C in dark with gentle agitation and the biotinylated RNA was recovered by ethanol precipitation ...
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bioRxiv - Physiology 2021Quote: ... PS exposure was detected using CF 594–tagged Annexin V (AnV, Biotium, # 29011). To monitor Ca2+ and PS dynamics before stimulation ...
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bioRxiv - Biophysics 2020Quote: ... Human Transferrin – CF488A (Biotium) at 130 nM ...
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bioRxiv - Molecular Biology 2020Quote: ... and the remaining 4sU-labeled nascent RNA was biotinylated using MTSEA-Biotin-XX (Biotium, 90066) as previously described67,81 ...
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bioRxiv - Microbiology 2020Quote: ... and the remaining 4sU-labeled nascent RNA was biotinylated using MTSEA-Biotin-XX (Biotium; 90066) as previously described83 ...
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bioRxiv - Molecular Biology 2023Quote: ... 4sU-RNA was then biotinylated using 0.1 mg/ml MTSEA biotin-XX linker (Biotium, #BT90066) for 30 min at room temperature in the dark with rotation ...
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bioRxiv - Biophysics 2024Quote: ... the cells were incubated in AnV buffer (1:175 dilution of the fluorescently tagged AnV (Biotium) in Hank’s balanced salt solution ...
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bioRxiv - Molecular Biology 2020Quote: ... 4tU-labeled RNA was biotinylated with 1 mg/mL MTSEA Biotin-XX (Biotium, Fremont, CA, #90066) for 30 min at room temperature in biotinylation buffer (20 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... poly(A) RNA was biotinylated with activated disulfide methane thiosulfonate (MTS) biotin (Biotium Biotin-XX MTSEA). 4tU-containing RNA was enriched as previously described (Duffy et al ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μg of RNA was biotinylated with 4 μg of MTSEA Biotin XX (Biotium Cat# 90066). The biotinylated RNA was isolated by binding to 80 μl of a Dynabeads MyOne Streptavidin C1 bead suspension (Invitrogen Cat# 65001 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... ; Blots were washed 3 times in TBS/0.1% Tween and incubated with secondary IRD-tagged antibodies (CF770, Biotium) diluted 1:10,000 in Odyssey blocking buffer for 1 h at room temperature.
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bioRxiv - Molecular Biology 2023Quote: ... RNA was denatured at 60°C for 10 min and biotinylated using 5 µg/mL biotin-MTS (Biotium) in 20% dimethylformamide (Sigma) ...
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bioRxiv - Biophysics 2023Quote: ... After removal of excess NeutrAvidin the flow cell is incubated with 150 pM biotinylated NTA beads (#90074, Biotium) for 3 minutes ...
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bioRxiv - Molecular Biology 2021Quote: RNA was biotinylated using 40 μl (~40 μg) total RNA and 4 μg MTSEA biotin-XX (Biotium #90066-1) in the following reaction ...
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bioRxiv - Immunology 2020Quote: ... sialic acid residues at the cell surface were oxidized using sodium meta-periodate (Thermo) and biotinylated with aminooxy-biotin (Biotium). Following quenching ...
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bioRxiv - Cell Biology 2023Quote: ... The RNA was denatured at 60C for ten minutes before being biotinylated by the addition of 5ug/ml biotin-MTS (Biotium) in 20% dimethylformamide (Sigma) ...
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bioRxiv - Cell Biology 2019Quote: ... The Calbryte-590 loaded cells were then incubated in HBSS buffer containing 0.5 µg/mL CF 488-tagged-Annexin-V (Biotium, cat. 29011), a fluorescence PS probe ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were removed by centrifugation (3000 x g for 1 minute) and lysates split equally and incubated with either methanol (vehicle) or 1 μM biotinylated phalloidin (Biotium, 00028) for 2 hour at 4°C on a rotator ...
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bioRxiv - Neuroscience 2022Quote: ... Human transferrin-CF488A conjugate was purchased from Biotium (cat. #00081). Mouse monoclonal anti-GFAP (cat ...
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bioRxiv - Cell Biology 2023Quote: ... anti-mouse and anti-human IgG coupled to CF488R (Biotium) or CF640R (Biotium ...
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bioRxiv - Biophysics 2022Quote: ... The protein band was stained by One-Step Blue Protein Gel Stain (Biotium, Fremont, CA).
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bioRxiv - Neuroscience 2022Quote: ... or 10 μM fluorescently labeled human transferrin (Tf488; Biotium, cat. #00081) for 4 h at 37 °C under gentle agitation ...
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bioRxiv - Genomics 2022Quote: ... Total protein level of each lane was normalized by VersaBlot™ Total Protein Normalization Kits (Biotium Cat. # 33025).
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bioRxiv - Molecular Biology 2020Quote: ... Autofluorescence quenching: Human sections were treated with TrueBlack Lipofuscin Autofluorescence Quencher (TLAQ) (Biotium) per manufactures instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Labeling of protein carbonyl groups was obtained by overnight incubation of 20μg protein extracts with CF555DI hydrazide (Biotium, Fremont, CA, USA, #92165) at 4°C under medium agitation ...
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bioRxiv - Neuroscience 2022Quote: ... Human neuronal cultures were fixed on coverslips in 4%PFA (catalog#22023, Biotium, Fremont, CA, USA) for 1h at RT and then blocked for 1.5h at RT in 0.1% Triton-X and 10% horse serum (catalog#H0146 ...
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bioRxiv - Developmental Biology 2023Quote: ... explants were incubated with fluorescently conjugated human transferrin-594 (CF 594; 25 μg/mL; Biotium Fremont CA) for 40 minutes ...
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bioRxiv - Immunology 2021Quote: ... stained with one-step blue protein gel stain (Biotium, ref. 21003), and by SEC-HPLC with an xbridge protein BEH SEC (Waters ...
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bioRxiv - Microbiology 2024Quote: ... and 1/1000 Alexa FluorTM 488-conjugated cross-absorbed goat anti-human IgG (heavy chain specific) (Biotium, 20444). The plates were incubated for 30 min at 37°C in the dark ...
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bioRxiv - Microbiology 2024Quote: ... the secondary antibody 1/1000 Alexa Fluor 647TM-conjugated goat anti-human IgG (gamma chain specific) (Biotium, 20448) in PBS/1% BSA contained 1/2500 of VybrantTM DyeCycleTM Violet stain and 20 μg/ml Ethidium Bromide (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Protein gels were transferred onto nitrocellulose membranes and subsequently stained with Ponceau S (Biotium) before blocking in 5% BSA (Sigma ...
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bioRxiv - Biochemistry 2022Quote: Thermal melt measurements were using a GloMelt Thermal Shift protein stability kit from Biotium on a Qiagen Rotor-gene Q qPCR instrument ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... protein expression of the FRHΔ100 plasmid was induced with 0.2 mM IPTG (Biotium, 10021), at 18°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... the gels were re-stained with One-Step Lumitein™ Protein Gel Stain (Biotium) as per manufacturer’s directions and imaged again as before.
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bioRxiv - Genetics 2023Quote: ... Total protein stain was then conducted by diluting CF568-labeled succinimidyl ester (Biotium, 92131) at 1:100,000 in water containing 0.1 M NaHCO3 and 25 mM Na2CO3 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... pH 7.9) and subjected to SDSCPAGE followed by Lumitein Protein Gel Stain (Biotium, Hayward, CA, USA) and immunoblotting ...
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bioRxiv - Bioengineering 2023Quote: The melting temperature of purified nanoCLAMPs was determined using GloMelt Thermal Shift Protein Stability Kit (Biotium) per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Viability of cardiac PCs and CAECs exposed to 1 μg/mL (5.8 nM) S protein or PBS vehicle was evaluated using the Viability/Cytotoxicity Assay Kit (Biotium #30002), according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... one mg of eluted protein at a concentration of 50-150 µM was incubated with a 2× molar excess of CF660R (Biotium) or Cy3B (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... all proteins were used at a final concentration of 5 µM for this assay and GloMelt™ Thermal Shift Protein Stability Kit (Biotium) was used at final concentration of 0.5X ...
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bioRxiv - Immunology 2020Quote: ... Unconjugated primary antibodies CD23 and IgM were conjugated with Mix-n-Stain fluorescent protein tandem dyes antibody labeling kit for APC-CF750T (Biotium, Fremont, CA, USA) and Mix-n-Stain cf dye antibody labeling kit for CF405M (Biotium ...
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bioRxiv - Bioengineering 2022Quote: ... according to the manufacturer’s protocol and purified as described previously 45.The ACE2 or DnbALFA proteins were labeled by CF®640R succinimidyl ester dye (Biotium, USA, cat. 92108) in 0.1 M bicarbonate buffer using a 1:4 protein to dye ratio ...