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1 - 14 of 14 citations for Lactose operon repressor LacI E.coli His since 2019

• APE1 recruits ATRIP to ssDNA in an RPA-independent manner to promote the ATR DNA damage response

  Yunfeng Lin, et al., bioRxiv - Molecular Biology 2022

  Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
• Comparing the folding landscapes of evolutionarily divergent procaspase-3

  Liqi Yao, A. Clay Clark, bioRxiv - Biophysics 2022

  Quote: ... His-bind resin was purchased from VWR.
• Systematic part transfer by extending a modular toolkit to diverse bacteria

  Kevin Keating, Eric M. Young, bioRxiv - Synthetic Biology 2023

  Quote: ... Routine culture of E.coli for cloning purposes was performed in Miller’s LB Broth (Fisher BP1426-2) in 14mL Falcon tubes (VWR 60819-761) at 37°C on a rotating drum ...
• RNA promotes the formation of spatial compartments in the nucleus

  Sofia A. Quinodoz, et al., bioRxiv - Genomics 2020

  Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
• A single-cell method to map higher-order 3D genome organization in thousands of individual cells reveals structural heterogeneity in mouse ES cells

  Mary V. Arrastia, et al., bioRxiv - Molecular Biology 2020

  Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
• 3D genome organization around nuclear speckles drives mRNA splicing efficiency

  Prashant Bhat, et al., bioRxiv - Molecular Biology 2023

  Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
• Dual-sensing genetically encoded fluorescent indicators resolve the spatiotemporal coordination of cytosolic abscisic acid and second messenger dynamics in Arabidopsis

  Rainer Waadt, et al., bioRxiv - Plant Biology 2019

  Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
• A Humanized Yeast System for Evaluating the Protein Prenylation of a Wide Range of Human and Viral CaaX Sequences

  Emily R. Hildebrandt, et al., bioRxiv - Biochemistry 2023

  Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
• Extracellular matrix deposition precedes muscle-tendon integration during murine forelimb morphogenesis

  Yue Leng, et al., bioRxiv - Developmental Biology 2022

  Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
• HSV-1 ICP22 is a selective viral repressor of cellular RNA polymerase II-mediated transcription elongation

  Nur F. Isa, et al., bioRxiv - Molecular Biology 2021

  Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
• Xist spatially amplifies SHARP recruitment to balance chromosome-wide silencing and specificity to the X chromosome

  Joanna W. Jachowicz, et al., bioRxiv - Molecular Biology 2021

  Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
• Epistasis Arises from Shifting the Rate-Limiting Step during Enzyme Evolution

  Christopher Fröhlich, et al., bioRxiv - Evolutionary Biology 2023

  Quote: ... containing 6-His tag and TEV cleaving site.19 In 50 mM HEPES (VWR, PA, USA), pH 7.5 including 50 mM potassium sulfate (Honeywell ...
• GlyConnect: a glycan-based conjugation extension of the GlycoDelete technology

  Wander Van Breedam, et al., bioRxiv - Bioengineering 2021

  Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
• A multiplex platform to identify mechanisms and modulators of proteotoxicity in neurodegeneration

  Samuel J. Resnick, et al., bioRxiv - Neuroscience 2022

  Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...