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Citations for VWR :
401 - 450 of 1244 citations for 3 1 Pyrrolidinylmethyl phenyl magnesium bromide solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... the inactivating solution was replaced by pre-warmed plating medium (10% horse serum (#S900-500, VWR International GmbH, Darmstadt, Germany), 1.8 mM glutamine ...
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bioRxiv - Microbiology 2022Quote: ... after electrophoresis gels were immersed into a methanol/sodium acetate (pH=5) solution and incubated at room temperature on rocking platform (VWR) at 1 speed for 2 min with constant shaking ...
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Label-free digital holotomography reveals ibuprofen-induced morphological changes to red blood cellsbioRxiv - Cell Biology 2022Quote: ... Ibuprofen powder was obtained by crashing a 400 mg ibuprofen tablet and stock solutions were prepared by dissolving ibuprofen (2 mg/mL) in 2 mL of PBS (VWR). Four concentrations of ibuprofen solution were prepared (0.25 mM ...
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bioRxiv - Microbiology 2023Quote: ... gels were immersed in a methanol-sodium acetate solution (pH 5) and incubated at room temperature on a rocking platform (VWR) at a speed of 1 for 2 min ...
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bioRxiv - Cell Biology 2024Quote: ... This was followed by perfusion with Solution 2 consisting of DMEM supplemented with collagenase type I (0.8 mg/mL) (VWR International) for 7–8 mins at the same flow rate ...
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bioRxiv - Microbiology 2024Quote: ... The calcium chloride concentration in KGM was maintained at 0.15 mM by supplementing additional CaCl2 (Calcium Chloride Solution, 0.5 M, VWR, Radnor, PA). Cells were maintained at 37°C and 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... slides were warmed to room temperature prior to antigen retrieval in a 1X antigen retrieval solution (VWR, Cat# 103780-314) for 5 min at 80°C ...
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bioRxiv - Microbiology 2024Quote: ... The calcium chloride concentration was maintained at 0.15 mM by supplementing additional CaCl2 (Calcium Chloride Solution, 0.5 M, VWR, Radnor, PA). For all hTCEpi cell experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... Beads were formed by dripping the cell suspension into a sterile 100 mM CaCl₂ solution (VWR, Fontenay-sous-Bois, France). The beads were subsequently washed in 0.15 M NaCl solution (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: Embryonic tissue samples from different embryonic days were fixed overnight at room temperature in 4% paraformaldehyde solution (VWR, Pennsylvania, USA), followed by dehydration and embedding in paraffin ...
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bioRxiv - Microbiology 2020Quote: ... were cut with a cryostat and 3 sections of 20 µm were placed on each microscope glass slide (VWR). Sections were fixed with acetone for 10 minutes ...
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bioRxiv - Synthetic Biology 2020Quote: ... 3-D spheroids were generated by seeding 2.0E6 cells per well of a 6-well cell repellent plate (VWR) in a final volume of 4.0 mL complete media and placed on an orbital shaker at 95 RPM for 24 hours ...
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bioRxiv - Synthetic Biology 2020Quote: 3 biological replicates of each isolate were grown overnight in 96-deep-well plates (VWR International, cat #10755-248) in LB media ...
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bioRxiv - Biochemistry 2021Quote: Custom Eppendorf tubes for extract recovery were created by cutting approximately 3 mm from the bottom of a standard 0.5 mL Eppendorf tube (VWR). The tubes were then soaked in 70% ethanol to remove small molecule contaminants ...
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bioRxiv - Neuroscience 2023Quote: ... all odors in this study were presented from a 10-3 (0.1%) dilution in paraffin oil (J.T. Baker, VWR #JTS894), with the exception of movies collected from one fly ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was washed 3 times in PBS before blocking for 30 minutes room temperature with PBS+0.1% Triton X-100 (VWR) with 5% donkey serum (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... Blood for each animal was collected from the dorsal artery using 2-3 heparin coated capillary tubes (VWR®).
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bioRxiv - Microbiology 2024Quote: ... Plates were then washed 5 times in PBS before addition of 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) substrate (VWR), and incubation at 37°C for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Developmental Biology 2022Quote: ... The primary and the secondary antibodies were prepared in the blocking agent consisting of 0.1% Triton X-100 and 3% goat serum (VWR). Followed by washing the organoids with PBS 3x5min on a rocker ...
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bioRxiv - Bioengineering 2024Quote: ... The media collected from 3 flasks (25mL/T-175flask) was filtered through a 0.2μm filter (VWR, Cat#10040-464) followed by first round of ultracentrifugation (Thermo Scientific™ Sorvall™ WX ultraCentrifuges ...
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bioRxiv - Molecular Biology 2024Quote: Purified chromosomes 3 or 19 were cytocentrifuged (Cytospin3, Shandon) at 163 g for 10 min onto Polysine slides (VWR). After mounting with Vectashield containing DAPI (Vector Laboratories) ...
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bioRxiv - Neuroscience 2022Quote: ... The fixed tissue was placed in sucrose solution (30% sucrose in PBS) for 24h and frozen in tissue mounting medium (OCT mounting media, VWR chemicals). The tissue was coronally sectioned at 50-60µm thickness on a cryostat ...
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bioRxiv - Bioengineering 2021Quote: ... cell suspensions were prepared at a concentration of 300,000 cells/mL in 1X phosphate buffered saline (PBS) solution supplemented with 2% fetal bovine serum (FBS, VWR 89510-186) to reduce cell-cell and cell-PDMS adhesion ...
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bioRxiv - Cell Biology 2021Quote: ... The tissue was then incubated in 4% PFA/PBS overnight and either immersed in serial solutions of 15% and 30% sucrose/PBS and embedded in OCT (VWR Chemicals) for cryosection preparations or in 2% agarose for sectioning with a vibratome (Leica) ...
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bioRxiv - Pathology 2022Quote: ... We extracted the brains and post fixed each in the same fixative overnight and then placed them in a freeze protective solution containing 2% DMSO and 20% glycerine (PB with dimethyl sulfoxide, VWR Int. LLC, Cat# 23486.297, and glycerine, VWR, Cat# 24387.292) in a refrigerator until sectioning ...
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bioRxiv - Cell Biology 2022Quote: ... fixed breast samples were impregnated with 30% Sucrose solution in 1X PBS for at least 48h and embedded in OCT (VWR, #361603E). 70 μm-thick sections were incubated with 0.5% Triton-blocking buffer (5% FBS and 2% BSA in 1X PBS ...
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bioRxiv - Neuroscience 2020Quote: ... followed by cryoprotection in graded sucrose solutions (10 %, 20 %, 30 %) and embedded in cryomatrix (Tissue-Tek® O.C.T. Compound, Sakura® Finetek, VWR, 4583). Radial sections (14 μm thick ...
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bioRxiv - Developmental Biology 2020Quote: ... each embryo was positioned in a sagittal orientation in a tissue base mold (Sakura, 4162) in optimal cutting temperature compound (OCT) solution (Sakura, 4583) and frozen in a dry ice isopropanol (VWR, 20842) and stored at −80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... followed by cryoprotection in graded sucrose solutions (10%, 20%, 30%) and embedded in cryomatrix (Tissue-Tek® O.C.T. Compound, Sakura® Finetek, VWR, 4583). Radial sections (14 µm thick ...
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bioRxiv - Cancer Biology 2021Quote: Glass-bottom 6-well dishes (#0 thickness, IBL, #220.200.020) were treated with a bind-silane solution consisting of PlusOne Bind-Silane (VWR, #17-1330-01) and acetic acid (Pancreac Quimica ...
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bioRxiv - Systems Biology 2023Quote: ... and excess gel was washed away with 5 Milli-Q purified water washes and 5 Dulbecco’s Phosphate Buffered Saline (DPBS) solution (VWR L0119-0500) washes ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was then neutralized using 1M HEPES Solution pH 8.0 to a neutral pH before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ~2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). Next ...
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bioRxiv - Genetics 2023Quote: ... Starved flies were transferred to vials containing 600μl of Paraquat solution or 5% sucrose (mock treatment) as well as a circular Whatman filter paper (VWR, 89013-946) on top of a foam biopsy pad (Neta Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then neutralized using 1 M HEPES solution pH 8.0 before buffer exchange into 50 mM HEPES solution pH 7.9 and then concentrated to ∼ 2 g/L using a 10 kDa MWCO spin column (VWR, Radnor, PA). After ...
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bioRxiv - Molecular Biology 2024Quote: Arabidopsis thaliana Col-0 seeds were surface sterilized for 10 min in a 50% bleach solution with Tween 20 (VWR 0777A) followed by three washes with sterile dH2O ...
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bioRxiv - Microbiology 2024Quote: ... The pigmented extract was dissolved in 99.5% methanol and then 10 mL of the solution was poured into a clean cuvette and inserted into a P9 double beam UV-visible spectrophotometer (VWR, China) which directs a beam of light through the sample using methanol as negative control ...
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bioRxiv - Microbiology 2024Quote: ... a resazurin solution was prepared by dissolving one tablet of Resazurin (Resazurin Tablets for Milk Testing; Ref. 330884Y VWR International Ltd) in 30 mL of sterile PBS (phosphate buffered saline) ...
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bioRxiv - Cell Biology 2020Quote: ... Residual lipids and proteins were cleared from the extracts by spin filtration in 3-kDa-molecular-weight spin cut-off filter (VWR) added 3 mg C18-material (Waters) ...
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bioRxiv - Synthetic Biology 2022Quote: Single colonies were picked and cultured for 24 hours at 30 °C in 3 mL YPD liquid medium supplemented with appropriate antibiotics in 14 mL culture tubes (VWR) as seed culture ...
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bioRxiv - Physiology 2020Quote: ... adi14-3-3ζKO mice were fasted for six hours and challenged with d-glucose (2 g/kg b.w.; VWR, Solon, OH) by intraperitoneal administration (34 ...
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bioRxiv - Bioengineering 2020Quote: ... The PDMS slab was bonded to a 2-in by 3-in glass slide (VWR micro slides, cat. #48382-179) after plasma treatment (Harrick Plasma PDC-001 ...
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bioRxiv - Bioengineering 2021Quote: ... formulations were aliquoted 150 μL per well (n = 3/group) in a clear 96-well plate and sealed with optically clear and thermally stable seal (VWR). The plate was incubated in a microplate reader (BioTek SynergyH1 microplate reader ...
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bioRxiv - Microbiology 2020Quote: ... and 3 μL of the culture was inoculated into 3 mL of MSgg liquid media in a 6-well or 12-well microtiter plate (VWR). The plates were incubated at 30°C for 2-3 days ...
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bioRxiv - Microbiology 2020Quote: ... or 0.4 µM (cesA) of each primer (Table 3) and 60 ng of DNA (measured by a MySpec spectrophotometer, VWR International). qPCR reaction conditions for the panC detection followed the master mix supplier’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were washed with PBS 3 times and fixed and permeabilized with 3.7% formaldehyde and 0.3% Triton-X (VWR 80503-490) in PBS for 30 minutes at room temperature ...
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bioRxiv - Genetics 2022Quote: ... Pre-denaturation was performed at 91°C for 3 min and then 60°C for 20 min on heat blocks (VWR). After plates were spun ...
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bioRxiv - Microbiology 2021Quote: ... The PDMS slab was bonded to a 2-in by 3-in glass slide (VWR micro slides, cat. #48382-179) after plasma treatment (Harrick Plasma PDC-001 ...
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bioRxiv - Genetics 2022Quote: ... and an equal volume of 1x Sperm Salts with 3% paraformaldehyde was applied for 5 min before samples were affixed to a Superfrost Plus slide (VWR). For both protocols ...
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bioRxiv - Genetics 2022Quote: ... Predenaturation was performed at 91 °C for 3 minutes and then 60 °C for 20 minutes on heat blocks (VWR). After plates were spun ...