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Citations for Zymo Research :
1 - 47 of 47 citations for Siglec 15 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from HEK293 and CDK9as cells using a Quick-RNA Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Total RNA was extracted from mouse tissues and HEK293 cells using Quick-RNA MiniPrep Kit (ZYMO Research, Irvine, CA). Five μg of treated RNA was used as template for first-strand cDNA synthesis by using RevertAid RT Reverse Transcription Kit (Life Technologies ...
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bioRxiv - Genomics 2021Quote: ... 15 µl EZ Methylation Direct conversion reagent (Zymo Research) was added ...
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bioRxiv - Molecular Biology 2020Quote: ... the DNMT3A KO and WT HEK293 cells were cultured and RNA samples were extracted using Direct-zol RNA kit (Zymo Research; Irvine, CA, USA). Then cDNA was synthesized according to the protocol of the RT-PCR kit (Takara ...
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bioRxiv - Microbiology 2022Quote: The ZymoBIOMICS human fecal reference sample (Zymo D6323) was thawed at room temperature and 100 μL was taken and centrifuged at 35g for 20 minutes to separate cells from large fecal particles ...
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bioRxiv - Neuroscience 2019Quote: ... Methylated and unmethylated human gDNAs (Zymo Research, Irvine, CA) were bisulfite-converted using the EZ DNA Methylation-Gold kit (Zymo Research ...
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bioRxiv - Genomics 2021Quote: ... The Human Methylated & Non-methylated DNA Set (ZYMO Research) was used to generate DNA methylation standards ...
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bioRxiv - Genomics 2021Quote: ... Purified bisulfite-treated DNA was eluted in 15 mL of M-Elution Buffer (Zymo Research). DNA methylation levels were measured using Infinium Human Methylation 450 arrays (Illumina ...
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bioRxiv - Immunology 2020Quote: ... whole zebrafish larvae or human biopsies using TRI Reagent (Zymo Research) or TRIzol (Invitrogen ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction was stopped by proceeding to RNA purification with elution in 15 μL (R1013, Zymo Research). Polyadenylated RNA (1 μg ...
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bioRxiv - Synthetic Biology 2022Quote: ... 15 μl of PCR products were pooled and purified using PCR Purification Kit (D4013, Zymo Research Co.). Depending on the amplicon ...
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bioRxiv - Plant Biology 2019Quote: ... benthamiana plants was purified from upper non-inoculated leaves at 15 dpi using silica-gel columns (Zymo Research). For virus infection diagnosis ...
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bioRxiv - Genomics 2021Quote: RNA from human RPTECs was extracted using the Direct-zol MicroPrep Plus Kit (Zymo) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Cultured or purified human cells were lysed directly in RNA lysis buffer (Zymo Research) and homogenized by pipetting ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and bodies (thorax minus legs + abdomen) were dissected and pooled from 15 individuals in DNA/RNA shield reagent (Zymo) and stored at -20°C until further processing ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from primary human PTC with the Direct-zol MicroPrep Kit (Zymo) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... followed by a 15 minute treatment with DNase and subsequent purification with Direct-zol™ RNA MiniPrep Plus (Zymo Research). All shRNAs used in this study can be found in Supplementary Dataset 2.
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bioRxiv - Molecular Biology 2022Quote: ... The assembly is cleaned and concentrated to 15 μl mQ using the DNA Clean & Concentrator™-5 kit (Zymo, D4004). 3 μl Gel Loading Dye ...
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bioRxiv - Microbiology 2023Quote: ... 15 min) and total RNA was extracted and DNAseI-treated using the Direct-zol RNA miniprep plus kit (Zymo Research). RNA quality was verified by 1% agarose electrophoresis and quantified using Nanodrop One (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: CP-KO RNA was extracted from 15 3h PBF guts with the Direct-zol RNA Mini-prep kit (Zymo Research) including on-column DNase treatment and transcribed into cDNA with the qScript cDNA Synthesis Kit (Quantabio) ...
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bioRxiv - Genomics 2020Quote: ... 5 µl reactions were purified with the ZR-96 Oligo Clean & Concentrator and 15 µl reactions were purified with the Oligo Clean & Concentrator kit (Zymo Research) and eluted with probe resuspension buffer ...
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bioRxiv - Genetics 2021Quote: DNA samples ranging from 500 ng-2 μg were digested to free nucleosides using 5-15 U of DNA Degradase Plus (Zymo Research) in 25 μl reactions incubated for 2 hrs at 37 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The reactions were treated with DNAse (15 min at 37°C) and remaining RNA was recovered using Direct-zol RNA mini prep kit (Zymo research) according to the protocol ...
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bioRxiv - Biochemistry 2022Quote: ... The extracted RNA was treated with DNase I for 15 minutes at 37°C and further purified using a Quick-RNA miniprep kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... RNA was purified from a volume of lysate equivalent to 15 mg of tissue (525 uL) using a Quick-RNA Miniprep kit (Zymo Research). 500 ng of total RNA was used to generate libraries ...
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bioRxiv - Bioengineering 2023Quote: The 2 μL of EVs were incubated at room temperature for 15 min with 1 U of DNase I (Zymo Research) and 1x DNA Digestion Buffer ...
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bioRxiv - Bioengineering 2024Quote: Total RNA was extracted from SCD or HD HSPCs (15, 24, 48, 72 hours and 6 days post-transfection) using the Quick-DNA/RNA Miniprep (ZYMO Research). RNA was treated with DNase using the DNase I kit (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: The 10 μL of eEVs were incubated at room temperature for 15 min with 1 U of DNase I (Zymo Research) and DNA Digestion Buffer ...
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bioRxiv - Genomics 2022Quote: RNA from human RPTECs was extracted with the TRIZOL and Direct-zol MicroPrep Plus Kit (Zymo) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant was transferred to a new 15 mL falcon tube containing 3.25 mL of ammonium bisulfite solution (Zymo Research, product #5030) and heated to 98°C for 10 minutes ...
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bioRxiv - Genomics 2021Quote: ... 500 µL of supernatant was transferred to 15 mL falcon tube containing 3.25 mL of ammonium bisulfite solution (Zymo Research, product #5030) and shaken in a thermomixer at 98°C for 10 minutes (15s on/30s off) ...
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bioRxiv - Molecular Biology 2022Quote: ... The digested codon random mRFP with singlestranded overhangs was cleaned and concentrated to 15 μl mQ using the DNA Clean & Concentrator™-5 kit by Zymo (D4004). The complete 15 μl containing the codon random mRFP library was used in a ligation reaction to generate the plasmid library.
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bioRxiv - Genetics 2019Quote: ... Small RNAs were sized selected and purified from a 15% denaturing polyacrylamide-urea gel using the ZR small-RNA™ PAGE Recovery Kit (Zymo Research). For oxidized RNA library preparations ...
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bioRxiv - Genetics 2022Quote: ... 30 ng of purified dsDNA was tagmented at 55 °C for 15 min and purified using DNA Clean & Concentrator-5 kit (Zymo Research, D4004) The tagmented DNA was PCR amplified using Nextera primers ...
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bioRxiv - Physiology 2023Quote: ... and immediately lysed with TRIzol (Fischer Scientific, 15-596-018) for RNA isolation using the Direct-zol RNA MicroPrep Kit (Zymo Research, R2062) per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... Digestion was confirmed with methylated and unmethylated human DNA standards provided with the OneStep qMethyl kit (Zymo) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Bisulfite conversion was confirmed by PCR using Universal Methylated Human DNA Standard with hMLH1 Primers (Zymo Research). Converted DNA was then applied to an Illumina Infinium MethylationEPIC850 BeadChip (865918 probes ...
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bioRxiv - Immunology 2020Quote: ... chromatin was de-crosslinked for 15 minutes at 65°C and purified using the ChIP DNA Clean & Concentrator kit (Zymo Research, Freiburg, Germany). Binding of Msh2 ...
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bioRxiv - Systems Biology 2023Quote: ... The reactions were incubated for 15 min at 50 °C and 3 μl were transformed into Mix & Go! Competent Cells - DH5 Alpha (Zymo Research Corporation, Irvine, CA). Correct assembly was checked by colony PCR followed by Sanger sequencing of the gene fragment region and the Gibson junctions ...
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bioRxiv - Genomics 2019Quote: 200 ng of mouse E14 genomic DNA sheared to 15 kb fragments were treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (Zymo Research, Irvine, CA, USA Research), or an EpiTect Bisulfite Kit (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: For human OE and OB RNA was extracted from 10mg of tissue per sample using Direct-zol RNA kit from Zymo Research (Catalog #R2052) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from human iPSC-FBs and mouse Raw 264-7 cells using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instructions ...
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bioRxiv - Genomics 2023Quote: ... 350 ng of each extracted sperm DNA and 350 ng of control human NA12878 lymphoblastoid cell line genomic DNA (Coriell Institute) were bisulfite-converted using the Zymo EZ DNA Methylation kit (Zymo Research). The loci were amplified by PCR using the following primer sets ...
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bioRxiv - Cancer Biology 2023Quote: RNA from exponentially growing or senescent human and bowhead whale primary skin fibroblasts was isolated using the Quick-RNA MiniPrep kit (Zymo Research) according to manufacturer instructions.
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bioRxiv - Genomics 2019Quote: ... RNA was isolated from different number of murine splenic CD3 positive T cell samples and human oocyte samples by using the Direct-zol™ RNA MiniPrep Kit (ZYMO, cat. R2050). The extracted RNA samples were dissolved into 30 µl of nuclease-free water (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... Two representative loci per fungal strain and the human MLH1 for the control were amplified by PCR (ZymoTaq PreMix, Zymo Research, Irvine, CA) using specifically designed primers for amplification of the bisulfite-treated DNA (designed using the ‘Bisulfite Primer Seeker’ (Zymo Research ...
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bioRxiv - Genomics 2022Quote: Total RNA was isolated from LV apical core tissue and from beating human iPS cell derived cardiomyocytes using Quick-RNA Miniprep Plus (Zymo Research, Irvine, CA, USA). The qRT-PCR was performed using SYBR mix (Thermofisher ...