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Citations for Zymo Research :
1 - 38 of 38 citations for Recombinant Human Granzyme B His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... SD -His with uracil and 5FOA (Zymo Research) was used to analyze the phase separation effects of a toxic Ura3p activity.
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bioRxiv - Genetics 2020Quote: ... Tagged DNA was purified with the DNA Clean & Concentrator-5 kit (Zymo Research) eluting with 14 µl EB buffer (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: ... The tagged DNA was purified with the DNA Clean ConcentratorTM5 Kit (Zymo Research). The samples were pre-amplified with the Nextera Index kit and the Nextera DNA Library Prep kit (both from Illumina) ...
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bioRxiv - Immunology 2020Quote: ... The tagged DNA was purified using DNA clean and concentrator kit (Zymo research). Fluorescently labeled DNA was transferred on to Zeta probe membrane (Bio Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Tagged DNA fragments were purified using the Clean & Concentrator-5 Kit (ZYMO, D4014), then subjected to PCR amplification and double-sided bead purification (to remove primer dimers and larger than 1,000 bp fragments ...
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bioRxiv - Biochemistry 2020Quote: ... then loaded directly onto a Ni-NTA protein miniprep column (His Spin Protein Miniprep, Zymo Research). Protein was washed following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... Recombinant bacmid DNA was purified using the ZR Bac DNA Miniprep Kit (Zymo Research) and correctness of the transposed sequence was analyzed by PCR using pUC/M13 primers ...
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bioRxiv - Microbiology 2019Quote: ... The cells were harvested and the protein was purified using His-Spin Protein Miniprep kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Recombinant reporter constructs were isolated using the ZymoPURE™ Plasmid Miniprep Kit (Zymo Research, #D4210), following the manufacturer’s protocol with the following minor modifications ...
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bioRxiv - Microbiology 2022Quote: The ZymoBIOMICS human fecal reference sample (Zymo D6323) was thawed at room temperature and 100 μL was taken and centrifuged at 35g for 20 minutes to separate cells from large fecal particles ...
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bioRxiv - Microbiology 2022Quote: ... After collection of TI and TS fractions 100 µl of His-affinity gel (i.e., Ni-NTA Magnetic Agarose Beads) (ZYMO Research) and 10 µl of protease inhibitor cocktail (Sigma ...
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bioRxiv - Physiology 2023Quote: ... we used the Zymo RiboFree Total RNA Library Prep kit (R3003-B, Zymo Research, Irvine, CA) following manufacturer instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Methylated and unmethylated human gDNAs (Zymo Research, Irvine, CA) were bisulfite-converted using the EZ DNA Methylation-Gold kit (Zymo Research ...
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bioRxiv - Genomics 2021Quote: ... The Human Methylated & Non-methylated DNA Set (ZYMO Research) was used to generate DNA methylation standards ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA of sorted GC B cells were isolated by Quick-DNA Microprep Kit (Zymo Research; D3020) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... whole zebrafish larvae or human biopsies using TRI Reagent (Zymo Research) or TRIzol (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Total RNA was isolated from cultured B cells at indicated timepoints using the Quick-RNA Microprep Kit (Zymo Research). cDNA was synthesized with the RevertAid RT Kit (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... coli TOP10 cells were propagated in LB medium (ampicillin, 100 µg/ml) and used for recombinant plasmid DNA isolation (Zypy Plasmid Miniprep, Zymo Research). Prior to plasmid transformation to expression host E ...
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed donor DNA fragment and recombinant plasmid pYlCas9 were simultaneously transformed into the targeted strain using the Frozen-EZ yeast transformation II Kit (Zymo Research). The amounts of donor DNA and plasmid pYlCas9 used were 1 μg and 500 ng ...
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bioRxiv - Microbiology 2021Quote: ... One batch of B cells was then harvested immediately after infection (day 0 of infection) with TRI Reagent (Zymo Research) and the rest of the infected cells were kept in culture for 3 days at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The plasmids with the A/B standards were then extracted and purified using the ZymoPURE Plasmid Miniprep Kit (Zymo Research), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... in Lysing Matrix B tubes and RNA was purified with the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA). The quantity and quality of the RNAs were evaluated using Qubit RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: RNA from resting B cells isolated from three wild-type animals was isolated using Direct-zol RNA Miniprep Kit (Zymo). Libraries were constructed using the Illumina TruSeq Stranded Total RNA Library and sequenced on NextSeq2000 with PE100 sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... The linear A/B standards were finally treated with Proteinase K and further purified with the Zymo ChIP DCC-25 purification kit (Zymo Research). The final A/B standards were quantified using Qubit dsDNA HS Assay on Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... in Lysing Matrix B tubes and RNA was purified with the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA, United States). The quantity and quality of the RNAs were evaluated using Qubit RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: RNA from human RPTECs was extracted using the Direct-zol MicroPrep Plus Kit (Zymo) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Cultured or purified human cells were lysed directly in RNA lysis buffer (Zymo Research) and homogenized by pipetting ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from primary human PTC with the Direct-zol MicroPrep Kit (Zymo) following manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: RNA from human RPTECs was extracted with the TRIZOL and Direct-zol MicroPrep Plus Kit (Zymo) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Digestion was confirmed with methylated and unmethylated human DNA standards provided with the OneStep qMethyl kit (Zymo) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Bisulfite conversion was confirmed by PCR using Universal Methylated Human DNA Standard with hMLH1 Primers (Zymo Research). Converted DNA was then applied to an Illumina Infinium MethylationEPIC850 BeadChip (865918 probes ...
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bioRxiv - Microbiology 2022Quote: For human OE and OB RNA was extracted from 10mg of tissue per sample using Direct-zol RNA kit from Zymo Research (Catalog #R2052) ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from human iPSC-FBs and mouse Raw 264-7 cells using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instructions ...
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bioRxiv - Genomics 2023Quote: ... 350 ng of each extracted sperm DNA and 350 ng of control human NA12878 lymphoblastoid cell line genomic DNA (Coriell Institute) were bisulfite-converted using the Zymo EZ DNA Methylation kit (Zymo Research). The loci were amplified by PCR using the following primer sets ...
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bioRxiv - Cancer Biology 2023Quote: RNA from exponentially growing or senescent human and bowhead whale primary skin fibroblasts was isolated using the Quick-RNA MiniPrep kit (Zymo Research) according to manufacturer instructions.
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bioRxiv - Genomics 2019Quote: ... RNA was isolated from different number of murine splenic CD3 positive T cell samples and human oocyte samples by using the Direct-zol™ RNA MiniPrep Kit (ZYMO, cat. R2050). The extracted RNA samples were dissolved into 30 µl of nuclease-free water (Qiagen ...
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bioRxiv - Genetics 2020Quote: ... Two representative loci per fungal strain and the human MLH1 for the control were amplified by PCR (ZymoTaq PreMix, Zymo Research, Irvine, CA) using specifically designed primers for amplification of the bisulfite-treated DNA (designed using the ‘Bisulfite Primer Seeker’ (Zymo Research ...
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bioRxiv - Genomics 2022Quote: Total RNA was isolated from LV apical core tissue and from beating human iPS cell derived cardiomyocytes using Quick-RNA Miniprep Plus (Zymo Research, Irvine, CA, USA). The qRT-PCR was performed using SYBR mix (Thermofisher ...