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Citations for Zymo Research :
1 - 32 of 32 citations for RT qPCR Master Mixes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and 2 ng of RNA per sample was used in each qPCR reaction according to instruction in the ZymoScript Onestep RT-qPCR Kit (Zymo Research). qPCR was conducted using a QuantStudio3 (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA for RT-qPCR was isolated using Direct-zol RNA MiniPrep Kit (Zymo Research) for all other experiments RNA was isolated using standard Tri-reagent (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... and via RT-qPCR with a next generation sequencing (NGS) library quantification kit (Zymo Research, Irvine, CA). RNA sequencing (1 lane ...
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bioRxiv - Microbiology 2022Quote: ... or by RT-qPCR (IDT technologies, Charité kit) after RNA extraction with TRI reagent and Direct-Zol RNA miniprep kit (Zymo Research).
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bioRxiv - Microbiology 2020Quote: ... 75μl supernatants were collected at 48hpi and inactivated 1:1 in 1X DNA/RNA Shield for RNA extraction and RT-qPCR analysis (Zymo Research, Irvine, CA). RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... supernatants were collected at 48hpi and inactivated 1:1 in 1X DNA/RNA Shield for RNA extraction and RT-qPCR analysis (Zymo Research, Irvine, CA). RNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen ...
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bioRxiv - Synthetic Biology 2022Quote: ... and fragment mixes for homology dependent assembly (Figure 5) were cleaned and concentrated with DNA Clean & Concentrator-5 columns (Zymo), and DNA was eluted with 10 μL molecular grade water ...
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bioRxiv - Bioengineering 2023Quote: ... cDNA was synthesized from extracted RNA using ZymoScript RT PreMix kit (Zymo Research). 2 ng of the cDNA template was combined with 0.25 μM forward and reverse qPCR primers (Table S8) ...
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bioRxiv - Immunology 2023Quote: ... First-strand cDNA was synthesized by ZymoScript RT PreMix Kit (ZYMO Research, Cat#R3012). All gene expression results were expressed as an arbitrary unit (AU ...
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bioRxiv - Cancer Biology 2022Quote: Chromatin immunoprecipitation and qPCR was performed using the Zymo-Spin ChIP kit (Zymo, D5209) as previously described (24) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA for quantitative RT-PCR assays was isolated using the Quick-RNA Microprep kit (Zymo) with on-column DNase treatment per the manufacturer’s protocol.
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bioRxiv - Genomics 2023Quote: qPCR for bacterial DNA load was performed using the Femto Bacterial DNA Quantification kit (Zymo Research). 2 μL of eluate was used as the input ...
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bioRxiv - Developmental Biology 2022Quote: ... GATA-3 and RORC were generated by PCR with ZymoTaq PCR Master Mix (Zymo Research) using the primers listed in Table 1 ...
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bioRxiv - Cell Biology 2019Quote: ... washed twice with ice cold PBS and processed for qPCR using a RNA purification kit (Zymo Cat# D7011). 500 ng total RNA from each sample were reverse transcribed using Superscript™ III reverse transcriptase using an oligo(dT ...
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bioRxiv - Genetics 2024Quote: ... rDNA size was measured by qPCR using DNA that was extracted by YeaStar Genomic DNA Kit (Zymo Research) using the primers listed in Supplemental Table 2 ...
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bioRxiv - Neuroscience 2023Quote: ... knockdown efficiency was calculated via qPCR: RNA was extracted with the Zymo Direct-Zol RNA microprep kit (#R2062, Zymo); cDNA synthesis was performed with the SuperScript IV First Strand Synthesis System using oligo-dT priming (#18091050 ...
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bioRxiv - Microbiology 2023Quote: ... The negative controls had no or extremely low DNA concentrations to proceed to library preparation as determined by RT-PCR using the Femto Bacterial DNA Quantification Kit from Zymo and were therefore excluded from further analysis (n =10 ...
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bioRxiv - Microbiology 2024Quote: ... the PCR master mix has been treated with 0.02% (v/v) of DNAse I 1 U/mL (Zymo) at room temperature for 15 minutes ...
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bioRxiv - Microbiology 2019Quote: ... qPCR was used to quantify 16S rRNA gene abundances [40] using the Femto™ Bacterial DNA Quantification Kit (Zymo Research). Assays were conducted following manufacturer instructions using a BioRad CFX Connect™ Real-Time PCR Detection System ...
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bioRxiv - Molecular Biology 2022Quote: ... TurboDNase treatment as described above usually removes most genomic DNA and small fragments of DNA that could prime as random primers (detectable by ‘no RT primer’ control) are cleared away efficiently by the RNA Clean & Concentrator-5 kit (Zymo Research). Hence ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR products containing linked TCRα:β cDNA amplicons were purified using a DNA Clean & Concentrator Kit (D4033, Zymo Research, Irvine, CA).
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bioRxiv - Bioengineering 2023Quote: ... The columns were centrifuged for 4 min at 1500 x g and 1 min at 10000 x g at RT and incubated with 400 µl Genomic lysis buffer (Zymo Research) supplemented with RNase A (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... using bead-beating for 30 min at RT followed by purification using Direct-zol RNA Miniprep kit (Zymo Research; Tustin, CA). Eluted RNA was analyzed in a Nanodrop Lite (Thermo Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... The ITS regions were amplified by qPCR using ITS-specific primers provided by the Femto(tm) Fungal DNA Quantification Kit (Zymo Research) to quantify the relative abundance of fungal DNA following the manufacturer’s recommendations for the three biological replicates ...
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bioRxiv - Genomics 2021Quote: ... The libraries were pooled based on qPCR data and subjected to bisulfite conversion using the EZ DNA Methylation Direct Kit (Zymo Research) with changes to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA extracted from individual particle-attached communities was quantified through qPCR of the 16S rRNA gene using the Femto Bacterial DNA Quantification Kit (Zymo Research), which has a lower limit of detection of 20fg ...
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bioRxiv - Microbiology 2023Quote: ... the reaction was prepared in a volume of 20 µL consisting in 2 µL of the template DNA and 18 µL of Femto Bacterial qPCR Premix (Femto Bacterial DNA Quantification Kit, Zymo Research). The PCR reaction was performed using a 7500 Fast Real-Time PCR System (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... were thoroughly mixed 1:1 with 2X DNA/RNA Shield™ and incubated at RT for 20 min for inactivation and vRNA was extracted using the Quick-RNA™ Viral Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: MSP1-specific B cells that successfully expanded in culture were collected by centrifugation (5 min. at 250 × g and RT) and stored at -70°C in 50 µl Tri-Reagent (Zymo #R2050-1-200). Heavy and light chain variable regions were amplified from MSP1-specific B cells by cDNA synthesis and a series of PCR reactions ...
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bioRxiv - Cancer Biology 2019Quote: RNA was isolated from normal bladder tissues and from VM-CUB1 and RT-112 cells using Direct-zol RNA MiniPrep kits (Zymo Research, Irvine, CA). Next ...
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bioRxiv - Genomics 2020Quote: ... After PCR amplification with a Phusion Hot-start master mix and purification with a DNA Clean & Concentrator-5 kit (Zymo Research, D4013), probes were T7 amplified with a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England BioLabs ...
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bioRxiv - Systems Biology 2022Quote: ... the second sample was stored at RT for 3 – 5 days and the third sample was collected in a Zymo research tube (Zymo Research Inc., Irvine, CA, USA) containing 9ml DNA/RNA shield and stored at RT for 3 – 5 days before storage at −80 °C ...