Labshake search
Citations for 3M :
1 - 28 of 28 citations for Centrosomal Protein 63 kDa CEP63 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: Samples were incubated in primary antibody solution (primary antibody in 5% DMSO/0.3M Glycine/PTxwH) for 8 days ...
-
bioRxiv - Biochemistry 2019Quote: ... Fully deuterated samples were made by first denaturing the protein (3M guanidine HCl and high heat for at least 30 minutes) ...
-
bioRxiv - Developmental Biology 2023Quote: ... Columns for protein binding were prepared as follows: C18 Discs (Emmore 3M C18) were punched out using a blunted syringe needle and pushed into 200 μl tips before activating with methanol ...
-
bioRxiv - Neuroscience 2024Quote: ... and bound proteins were dissociated in 50 μL of 1x loading dye (0.3M Tris·HCl ...
-
Novel model of distal myopathy caused by the myosin rod mutation R1500P disrupts acto-myosin bindingbioRxiv - Molecular Biology 2019Quote: Protein lysates were prepared by homogenizing hindlimb muscle tissue in myosin extraction buffer (0.3M NaCl ...
-
bioRxiv - Immunology 2021Quote: ... The protein thus obtained was mixed with an equal volume of guanidine solution (3M GuHCl ...
-
bioRxiv - Genetics 2024Quote: Protein lysates or histone samples were mixed with 5X Loading Buffer (5% SDS, 0.3M Tris pH 6.8 ...
-
bioRxiv - Cancer Biology 2020Quote: Harvested cell culture pellets were resuspended in protein lysis buffer (25mM HEPES pH 7,5; 0,3M NaCl ...
-
bioRxiv - Cell Biology 2021Quote: ... for 30 minutes at room temperature and then incubated with primary antibody overnight at 4°C in antibody solution (2.5% BSA, 0.3M Glycine and 1% Tween in DPBS). Murine and human arterial sections were stained with primary antibodies [Supplementary Table I] ...
-
bioRxiv - Cell Biology 2021Quote: ... for 30 minutes at room temperature and then incubated with primary antibody overnight at 4°C in antibody solution (2.5% BSA, 0.3M Glycine and 1% Tween in DPBS). Murine and human arterial sections were stained with primary antibodies [Supplementary Table I] ...
-
bioRxiv - Developmental Biology 2022Quote: ... Bound protein complexes were trypsinized on bead and peptides were desalted with C18-STAGE tips (3M) as described previously (Zee et al. ...
-
bioRxiv - Developmental Biology 2022Quote: ... Protein eluates were dialyzed overnight at 4°C in dialysis buffer (0.1M Tris [pH 8.0], 0.3M NaCl) and concentrated using amicon ultra concentrators ...
-
bioRxiv - Biophysics 2023Quote: ... Different concentrations of protein samples were mixed with 4X Loading buffer (0.25 M Tris-HCl pH 6.8, 8% SDS, 0.3M DTT ...
-
bioRxiv - Systems Biology 2020Quote: ... Incubation with primary antibodies (0.3M glycine, 0.1% Tween 20, in PBS) was performed overnight in a humidified dark chamber at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... and resuspended in 50mL MURB protein sample buffer (100mM MES pH7.0, 1% SDS, 3M urea, 10% 2-mercaptoethanol) prior to Western analysis.
-
bioRxiv - Cell Biology 2020Quote: ... lysed in protein sample buffer (0.2M Tris HCl, 0.4 M DTT, 277 mM SDS, 6mM Bromophenol Blue, 4.3M glycerol), and boiled and subjected to SDS-PAGE and Western blotting as described above.
-
bioRxiv - Neuroscience 2024Quote: ... Resulting protein digests equivalent to 5 µg were desalted using StageTip (SDB-XC, 3M, St. Paul, MN, USA)51 ...
-
bioRxiv - Bioengineering 2019Quote: ... incubated with mouse anti-mKO1 monoclonal antibodies (M104-3M, 1/200; MBL) overnight ...
-
bioRxiv - Bioengineering 2019Quote: ... incubated with mouse anti-mKO1 monoclonal antibodies (M104-3M, 1/200; MBL), rabbit anti-M-opsin antibodies (AB5405 ...
-
bioRxiv - Systems Biology 2020Quote: ... and incubated with secondary antibodies (0.3M glycine, 0.1% Tween 20, in PBS) for 1 hour in a humidified dark chamber at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined with a BCA protein assay kit (23225, Thermo Fischer Scientific) and cell lysates were incubated with 5X Reducing Sample Buffer (0.3M Tris-HCL pH6.8 ...
-
bioRxiv - Microbiology 2023Quote: ... Proteins were eluted with ten column volumes of elution buffer (0.1M Tris HCl, pH 7.5, 0.3M Nacl, 250mM imidazole) in fractions of 1.0 ml in DNA/protease-free Eppendorf tubes ...
-
bioRxiv - Molecular Biology 2020Quote: Purified His6-KIAA1841(BTB) protein at 0.1mg/ml in sample buffer (10mM HEPES, pH8.0; 0.3M NaCl, 10% glycerol, 1mM TCEP) was crosslinked with 5-fold increasing concentration of glutaraldehyde (i.e ...
-
bioRxiv - Biochemistry 2021Quote: ... The pH of eluted antibodies was immediately neutralized using sodium acetate (3M, pH 9.0). After protein measurements at 280 nm ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were incubated at room temperature overnight in a solution of antibody diluent (0.3M NaCl ...
-
bioRxiv - Immunology 2021Quote: ... of MVA-HIV 1086.c Env construct (in a volume of 0.25 ml divided over left and right biceps) (35) and 15 μg 1086Δ7 gp120 K160N protein mixed with 3M-052 adjuvant in stable emulsion (3M-052-SE) (34 ...
-
bioRxiv - Neuroscience 2022Quote: ... Both primary and secondary antibodies were diluted in TNT (0.1M Tris-HCL, 0.3M NaCl, 0.5% Triton X-100). All confocal microscopy was performed on a Zeiss LSM 700 system ...
-
bioRxiv - Immunology 2021Quote: ... Additional high stringency washes were performed after the secondary antibody and Opal fluor applications using high-salt TBST (0.05M Tris, 0.3M NaCl, and 0.1% Tween-20, pH 7.2-7.6). TCT was used as the blocking buffer (0.05M Tris ...