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Citations for 3M :
1 - 49 of 49 citations for African Swine Fever Virus P30 Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: Melanoma cells (A375, MALME 3M, SKMEL-5) were seeded at a concentration of 4x104 per well in a 6 well plate format ...
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bioRxiv - Immunology 2022Quote: ... At 24 hpi cells were harvested to collect intracellular RNA by lysing the cells in 100 μl GITC reagent (3M GITC ...
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bioRxiv - Genomics 2022Quote: ... cells were washed in 2XSSCT/50% formamide (0.3M NaCl ...
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bioRxiv - Developmental Biology 2022Quote: ... Cell lysis was performed with a lysis buffer (0.3M sucrose ...
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bioRxiv - Cell Biology 2023Quote: Cells were seeded into 150 mm dishes (3M/dish) and allowed to grow until near confluent ...
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bioRxiv - Genomics 2024Quote: ... 4f-SAMMY-seq (4f) starting from 3million cells (3M), 4f-SAMMY-seq starting from 50,000 or 10,000 cells (50k and 10k ...
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bioRxiv - Cancer Biology 2024Quote: ... melanoma cells (A375, B16, BLM, G361 and Malme-3M), lung cancer cells (A549 ...
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bioRxiv - Molecular Biology 2022Quote: PCa cell lines (PC-3, PC-3M, DU145, and DUTXR) were plated in a white-welled luminometer 96-well plate at a seeding density of 2×103 cells/well and treated with indicated concentrations of FK866 as a single agent ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed for 30min shaking in NP lysis buffer (3M Tris-HCl pH8 ...
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bioRxiv - Bioengineering 2020Quote: ... or 3 ×106 viable cells per device (0M, 1M, 3M, respectively). Excess media flowed through the semi-permeable hollow fibers while cells remained within the extraluminal space of the reactor ...
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bioRxiv - Molecular Biology 2023Quote: ... in brief: cells were resuspended in 1mL of Buffer 1 (0.3M Sucrose ...
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bioRxiv - Cell Biology 2022Quote: ... For cilia rescue experiments in LRP5/6DKO 293T cells (Figure 3M), LRP6 (WT and CTS mutants ...
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bioRxiv - Genomics 2023Quote: ... 1 million cells were treat with 1.5mL of Nun Buffer (0.3M NaCl ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were lysed for 30min on ice with classic lysis buffer (0.3M NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed once with SSCx2 (0.3M sodium chloride, 30mM sodium citrate), followed by incubating with Wash buffer (SSCx2 with 10% formamide) ...
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bioRxiv - Genomics 2023Quote: ... 85M K562 cells per replicate were seeded in three T225 flasks (28.3M per flask), incubated for 24 hours ...
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bioRxiv - Physiology 2024Quote: ... Cells were placed in Ringer’s solution during imaging with perfluorocarbon (FC-770, 3M FluorinertTM) added mucosally to prevent ASL evaporation ...
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bioRxiv - Cancer Biology 2020Quote: Harvested cell culture pellets were resuspended in protein lysis buffer (25mM HEPES pH 7,5; 0,3M NaCl ...
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bioRxiv - Molecular Biology 2022Quote: Melanoma cells (M14, MDA-MB-435, M19-Mel, SK-Mel-5, UACC-257, Malme-3M obtained from the Department of Dermatology ...
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bioRxiv - Cell Biology 2019Quote: ... then cells were treated with RNase in 2XSSC buffer (0.3M NaCl, 30mM Sodium Citrate, pH7.0), dehydrated and denatured under 72°C following cold ethanol wash ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were incubated in 2x SSC-T (3.0M NaCl, 0.3M Sodium Citrate, 0.1% Tween 20) for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were lysed with Guanidine lysis buffer (6M Guanidine-HCl, 50mM NaHPO4 buffer pH:8, 0.3M NaCl ...
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bioRxiv - Neuroscience 2019Quote: ... Then the cells were incubated with blocking buffer (1XPBS, 1% BSA, 0.3M glycine, 0.1% Tween 20) supplemented with 5% goat serum for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Samples were kept on ice and plated on aerobic cell count Petrifilm (3M Nelson Jameson Company) within 24 hours of collection ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated in permeabilization solution (0.1% TritonX-100, 5% serum, 0.3M glycine in 1xPBS) for 1hr at RT ...
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bioRxiv - Neuroscience 2019Quote: ... PC12 cells were lysed in ice-cold gradient buffer (0.3M NaCl, 1mM MgCl2, 15mM Tris-HCl (PH7.4), 0.1mg/mL cyclohexamide and 1mg/mL heparin ...
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Engineering of a fluorescent chemogenetic reporter with tunable color for advanced live-cell imagingbioRxiv - Molecular Biology 2021Quote: HeLa cells were treated with MEM media containing the appropriate fluorogen (HBP-3,5DOM, HBP-3,5DM, HBP-3M, HBT-3,5DOM ...
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bioRxiv - Molecular Biology 2023Quote: ... Briefly: The cell pellet containing expressed untagged RAD15 was thawed in two volumes of lysis buffer (3M NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... and retrobead-expressing cells were quantified through 8 serial sections through the VP from 8 mice (5F, 3M).
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bioRxiv - Molecular Biology 2022Quote: ... The flow cell consists of two ~0.16mm thick coverslips and a 0.074 mm double-sided acrylic tape (9495MP, 3M). The inner bottom surface of the flow cell is coated with an hydrophobic solution (nitrocellulose or Sigmacote ...
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bioRxiv - Genomics 2023Quote: ... and using standard parameters for single cell libraries (--soloMultiMappers Unique --soloBarcodeReadLength 28 --soloType CB_UMI_Simple --soloUMIlen 12 -- soloCBwhitelist 3M-february-2018.txt --soloFeatures GeneFull --clipAdapterType CellRanger4 --outFilterScoreMin 30 --soloCBmatchWLtype 1MM_multi_Nbase_pseudocounts --soloUMIfiltering MultiGeneUMI_CR --soloUMIdedup 1MM_CR --readFilesCommand zcat --outSAMtype BAM Unsorted) ...
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bioRxiv - Developmental Biology 2024Quote: ... Tetraploid embryos were produced via electrofusion of 2-cell stage embryos isolated from CD1 mice (40V, 30µs with a 250 µm electrode chamber in 0.3M mannitol ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentrations were determined with a BCA protein assay kit (23225, Thermo Fischer Scientific) and cell lysates were incubated with 5X Reducing Sample Buffer (0.3M Tris-HCL pH6.8 ...
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bioRxiv - Biophysics 2020Quote: ... A flow cell was assembled by combining a coverslip and a quartz slide with double-sided adhesive tape (3M). For convenient buffer exchange ...
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bioRxiv - Neuroscience 2021Quote: ... was kept low by continuously recirculating (~2 L/min) through a vacuum degassing system with a Liqui-Cell G453 2.5 × 8 Membrane Contactor (3M Separation and Purification Sciences Division ...
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bioRxiv - Biochemistry 2022Quote: ... and a custom-made flow cell containing 1−2-μm-wide chromium barriers using two-sided tape (3M 665). Single-molecule fluorescent images were collected with an inverted Nikon Ti-E microscope using prism based TIRF microscopy ...
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bioRxiv - Biochemistry 2020Quote: ... and a custom-made flow cell containing 1-2-μm-wide chromium barriers using two-sided tape (3M 665). Single-molecule fluorescent images were collected with a prism TIRF microscopy-based inverted Nikon Ti-E microscope ...
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bioRxiv - Genetics 2023Quote: ... Fixed cells were incubated in blocking buffer (1% BSA, 2% FCS, 0.3M glycine and 0.1% Tween-20 in PBS) at room temperature for 1 hour ...
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bioRxiv - Biophysics 2020Quote: ... we cut custom three-cell flow chambers out of double-sided adhesive sheets (Soles2dance, 9474-08×12 - 3M 9474LE 300LSE) using a laser cutter ...
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bioRxiv - Genomics 2022Quote: ... Cells are then resuspended in 10ml Sucrose/Glycerol buffer (1:1) (Sucrose Buffer: 0.3M Sucrose; 1% Triton-X (Sigma Aldrich); 10mM HEPES (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed once with PBS and the media was replaced with imaging media (complete 3M without phenol red). Cells loaded with Fluo-4 were incubated at room temperature for 20 minutes before imaging ...
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bioRxiv - Genomics 2023Quote: ... Each library was then mapped against its corresponding genome with the 10X V3 cell barcode whitelist (3M-february-2018.txt) and using standard parameters for single cell libraries (--soloMultiMappers Unique --soloBarcodeReadLength 28 --soloType CB_UMI_Simple --soloUMIlen 12 -- soloCBwhitelist 3M-february-2018.txt --soloFeatures GeneFull --clipAdapterType CellRanger4 --outFilterScoreMin 30 --soloCBmatchWLtype 1MM_multi_Nbase_pseudocounts --soloUMIfiltering MultiGeneUMI_CR --soloUMIdedup 1MM_CR --readFilesCommand zcat --outSAMtype BAM Unsorted) ...
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bioRxiv - Bioengineering 2023Quote: ... with an interior carrier film for enhanced mechanical stability With a thickness (0.17 mm) less than the battery pack cells and interconnects (3M 9495LE). The double-sided adhesive sheet is cut (prior to removing the liner of both sides ...
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bioRxiv - Biophysics 2019Quote: ... we made custom three-cell flow chambers using laser-cut double-sided adhesive sheets (Soles2dance, 9474-08×12 - 3M 9474LE 300LSE), glass slides (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... Cell powder (~20g) was resuspended in lysis buffer (50 mM Tris-HCl, 200 mM NaCl, 0.3M sucrose, 20 mM imidazole, pH 8.0), and centrifuged at 14,000 RPM for 40 minutes at 4°C in a Thermo Scientific Fiberlite F14-14 × 50cy rotor ...
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bioRxiv - Microbiology 2021Quote: ... Gaussia luciferase activity was assessed 48 hrs after infection by combining cell culture media with Gaussia luciferase substrate (0.1M Tris pH 7.4, 0.3M sodium ascorbate, 10 μM coelenterazine). Luminescence was immediately measured by a BioTek Cytation5 plate reader.
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bioRxiv - Molecular Biology 2021Quote: ... cells were removed by centrifugation and cell-free supernatants were passed through a positively charged 3M™Zeta Plus™ depth filter (3M) for removal of negatively-charged contaminants ...
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bioRxiv - Neuroscience 2019Quote: ... and FACS was performed using Beckman Coulter MoFlo Cell Sorter with proper gate settings and doublet discrimination (Supplemental Figure 3M, Supplemental Figure 6A) at the Johns Hopkins Bloomberg School of Public Health Flow Cytometry Core ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets containing the expressed NP constructs were resuspended in lysis buffer (50mM NaH2PO4, pH 7.0, 500mM NaCl, 3M Urea, and 5mM β-mercaptoethanol), supplemented with 1 mM phenyl-methyl sulphonyl fluoride (PMSF ...