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Citations for Expression Systems :
1 - 46 of 46 citations for Respiratory syncytial virus RSV fusion protein exodomain human heterodimeric IgG1 Fc tag recombinant antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... A single bicistronic baculovirus encoding the human Gβ1 subunit with a N-terminal 6x His-tag and rhinovirus 3C protease site and untagged human Gγ2 subunit was generated using the BestBac method (Expression systems) in Spodoptera frugiperda (Sf9 ...
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bioRxiv - Biochemistry 2022Quote: ... human full-length CB1 containing a N-terminal FLAG tag and C-terminal histidine tag was expressed in Spodoptera frugiperda Sf9 insect cells with the baculovirus method (Expression Systems). Receptor was extracted using 1% lauryl maltose neopentyl glycol (L-MNG ...
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bioRxiv - Biochemistry 2021Quote: ... The virus titers were measured by flow cytometry assay (Expression Systems).
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bioRxiv - Biophysics 2021Quote: ... and the virus was prepared using the BestBac system (Expression System, LLC). N-terminal Flag-tagged human Gβ1 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were infected with recombinant baculovirus (BestBac Expression Systems) at a density of ∼4.0 × 106 cells per ml ...
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bioRxiv - Biophysics 2024Quote: ... The virus titer was determined by gp64-PE mouse anti-baculovirus antibody (Expression Systems, CA) using a Guava benchtop Flow Cytometer (Millipore ...
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bioRxiv - Biophysics 2022Quote: ... P0 virus was produced by transfection with FuGENE HD and BestBac 1.0 Linearized Baculovirus DNA (Expression Systems) followed by a viral amplification to P1 ...
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bioRxiv - Biochemistry 2020Quote: ... Expression was performed by transducing recombinant baculoviruses into Sf21 insect cells (Expression Systems). Cells were harvested by centrifugation and resuspended in 25 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... A recombinant baculovirus (P0) was produced in SF9 cells (Expression Systems, CA, USA) as described in the protocol of the BestBactm 2.0 Δ v-cath/ChiA Baculovirus Cotransfection Kit (Expression Systems ...
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bioRxiv - Cell Biology 2021Quote: ... First passage (P1) recombinant baculovirus was generated by transfecting Sf9 cells (Expression Systems) with bacmid DNA complexed using linear polyethylenimine (PEI MAX ...
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bioRxiv - Biophysics 2023Quote: ... recombinant FUS was expressed in Sf9 insect cells (Expression Systems, Cat#94-001F) for 72 h using the baculovirus expression system87 and produced as a C-terminal SNAP fusion with an N-terminal maltose-binding protein (MBP ...
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bioRxiv - Biophysics 2023Quote: ... and the P3 virus was used to infect Tni suspension insect cell culture (Expression Systems; Cat. 94-002S) for protein expression.
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bioRxiv - Biochemistry 2020Quote: ... an Avi tag and XbaI and used to generate Baculovirus using the BestBac method (Expression Systems). Spodoptera frugiperda (Sf9 ...
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bioRxiv - Biochemistry 2020Quote: ... A recombinant baculovirus for Gαs subunit was prepared using the BestBac™ (Expression Systems) strategy ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant P1 baculovirus DNA was transfected into and cultured in Sf9 cells (Expression System) in penicillin-streptomycin-free ESF 921 protein-free insect cell culture media (Expression Systems ...
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bioRxiv - Biochemistry 2020Quote: ... which was amplified once to give a V1 virus stock used to infect 1 l cultures of Sf9 cells (Expression Systems). Cells were grown in ESF 921 Insect Cell Culture Medium Protein Free (expression systems 96-001-01 ...
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bioRxiv - Biochemistry 2022Quote: ... and analysis of the recombinant bacmid were performed following the manufacturer’s instructions (ExpiSf Expression System, Gibco). ExpiSf9 cells were cultured in ExpiSf9 CD medium to a density of 5×106 cells/mL ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were infected (multiplicity of infection of 6 for each virus) with separate baculoviruses (prepared using the Bac-to-Bac Baculovirus Expression System, Invitrogen) containing the CXCL12LRHQ gene as well as the ACKR3 gene (residues 2-362 ...
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bioRxiv - Cell Biology 2019Quote: ... Protein-Free (Expression Systems), supplemented with Fetal Bovine Serum (2% final concentration).
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bioRxiv - Biophysics 2022Quote: ... Protein Free (Expression Systems) and seeded in a 6-well plate to produce baculovirus ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen ...
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bioRxiv - Biophysics 2019Quote: ... with N-terminal FLAG and C-terminal octahistidine tag was expressed in Spodoptera frugiperda Sf9 insect cells using the baculovirus method (Expression Systems) in the presence of the L-168,049 (Tocris Bioscience) ...
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bioRxiv - Biophysics 2020Quote: ... 1μg of recombinant bacmid DNA was combined in 1 mL of ESF-921 media (Expression Systems, Cat. #96-001-01) with 20 μL of Cellfectin II (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: Expression of recombinant RDR2 was optimized using the TEQC method (62) in which a 200 ml culture of Hi5 cells (Expression Systems, Inc) was infected at an estimated multiplicity of infection (eMOI ...
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bioRxiv - Biochemistry 2021Quote: ... His6-tagged human Gβ1and Gγ2 were expressed in Tni insect cells (Expression systems) using baculovirus as previously described ...
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bioRxiv - Bioengineering 2020Quote: The RBD (residues 319-541) of the SARS-Cov-2 S protein was expressed as a secreted protein in Spodoptera frugiperda Sf9 cells (Expression Systems) using the Bac-to-bac baculovirus method (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmid-DNA was isolated with the ZymoPURE Plasmid Midiprep (ZymoGenetics, Seattle, Washington, United States The HY5 protein was expressed with TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... and protein was expressed in Sf9 cells (Expression Systems) using the Bac-to-Bac Baculovirus expression system (Invitrogen) ...
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bioRxiv - Biophysics 2022Quote: ... The protein was expressed in Trichoplusia ni ovarian cells (Expression Systems) for 60-72 hrs maintained in ESF 921 Insect Cell Culture Medium (Expression Systems ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was expressed in the Sf9 insect cell (Expression system) in the ESF921 medium using the Bac-to-Bac baculovirus system ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were expressed in Trichoplusia ni High Five cells (Expression Systems) by baculoviral expression ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein expression was carried out in Spodoptera Frugiperda cells (Sf9, Expression Systems) using the Bac-to-Bac Baculovirus expression system (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... in penicillin-streptomycin-free ESF 921 protein-free insect cell culture media (Expression Systems) in the presence of PEI ...
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bioRxiv - Biochemistry 2024Quote: ... were cultured in protein free ESF 921 insect cell culture media (Expression Systems LLC). Bacmid preparations ...
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bioRxiv - Molecular Biology 2021Quote: HMCES-FLAG proteins were expressed in 250 mL suspension cultures of Sf9 cells (Expression Systems) by infection with baculovirus expressing rHMCES-FLAG for 72 hrs ...
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bioRxiv - Genetics 2021Quote: ... were transfected using PEI transfection methods and cultured in protein-free ESF921 media (Expression Systems, 96-001). For 6-well plate transfection ...
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bioRxiv - Biophysics 2023Quote: ... Sf9 cells were obtained from Expression Systems and maintained in protein-free cell culture media purchased from Expression Systems (Cat. no: 96-001-01) at 27°C with 135 rpm shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi5 cells grown in ESF-921 media (Expression Systems, Davis, CA, United States) at 27 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were maintained in protein-free cell culture media purchased from Expression Systems (Cat. no: 96-001-01) at 27°C with 135 rpm shaking ...
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bioRxiv - Biochemistry 2021Quote: ... Protein expression was induced by baculovirus infection of 6-8 liter cultures of Sf9 cells in ESF921 medium (Expression Systems) at a density of ~2 × 106 cells/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... were seeded per well in a 6-well plate in protein-free ESF 921 media (Expression Systems Cat# 96-001) with no additives or antibiotics ...
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bioRxiv - Cell Biology 2023Quote: ... Tni cells were used for large-scale protein expression and are maintained in ESF 921 serum free medium (Expression Systems).
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bioRxiv - Cell Biology 2023Quote: For expression of Nv-Osk and Nv-Vas proteins in S2 (Drosophila Schneider 2) cells (Expression Systems, cat # 94-005S), corresponding cDNAs were subcloned under control of Actin5C promoter (GenScript) ...
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bioRxiv - Immunology 2021Quote: ... The protein was expressed in 2 L of Sf9 cells at 2×106 cells/mL maintained in ESF921 media (Expression Systems) by adding 25 mL virus per liter of culture ...
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bioRxiv - Microbiology 2023Quote: ... large-scale expression cultures were performed with Hi-5 cells cultured in the protein free ESF 921 Insect Cell Culture Medium (Expression System) and infected with 0.8-1.0% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... with a multiplicity of infection of 5 of baculovirus at 1×106 cells per ml and incubated at 27°C for 70 hours in Protein-free Insect Cell Culture Medium (Expression Systems, 96-001-01) with penicillin/streptomycin ...