Labshake search
Citations for Expression Systems :
1 - 43 of 43 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
-
bioRxiv - Biochemistry 2021Quote: ... His6-tagged human Gβ1and Gγ2 were expressed in Tni insect cells (Expression systems) using baculovirus as previously described ...
-
bioRxiv - Biochemistry 2021Quote: ... A single bicistronic baculovirus encoding the human Gβ1 subunit with a N-terminal 6x His-tag and rhinovirus 3C protease site and untagged human Gγ2 subunit was generated using the BestBac method (Expression systems) in Spodoptera frugiperda (Sf9 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... were infected with recombinant baculovirus (BestBac Expression Systems) at a density of ∼4.0 × 106 cells per ml ...
-
bioRxiv - Biophysics 2020Quote: ... 1μg of recombinant bacmid DNA was combined in 1 mL of ESF-921 media (Expression Systems, Cat. #96-001-01) with 20 μL of Cellfectin II (Thermo Fisher ...
-
bioRxiv - Biochemistry 2019Quote: ... or GST-tagged BMAL1 PAS-AB were expressed in Sf9 suspension insect cells (Expression Systems). Sf9 suspension cells were infected with a P3 virus at 1.2 × 106 cells per milliliter and grown for 72 hours ...
-
bioRxiv - Biochemistry 2022Quote: ... High Five cells (Expression Systems) in suspension culture at a density of 1×106 cells/ml were infected with baculovirus (V1 ...
-
bioRxiv - Biophysics 2023Quote: ... High-five insect cells (Expression Systems) and Expi293F mammalian cells (Thermo Fisher) ...
-
bioRxiv - Biochemistry 2020Quote: ... Expression was performed by transducing recombinant baculoviruses into Sf21 insect cells (Expression Systems). Cells were harvested by centrifugation and resuspended in 25 mM HEPES pH 7.4 ...
-
bioRxiv - Microbiology 2020Quote: ... A recombinant baculovirus (P0) was produced in SF9 cells (Expression Systems, CA, USA) as described in the protocol of the BestBactm 2.0 Δ v-cath/ChiA Baculovirus Cotransfection Kit (Expression Systems ...
-
bioRxiv - Cell Biology 2021Quote: ... First passage (P1) recombinant baculovirus was generated by transfecting Sf9 cells (Expression Systems) with bacmid DNA complexed using linear polyethylenimine (PEI MAX ...
-
bioRxiv - Biophysics 2023Quote: ... recombinant FUS was expressed in Sf9 insect cells (Expression Systems, Cat#94-001F) for 72 h using the baculovirus expression system87 and produced as a C-terminal SNAP fusion with an N-terminal maltose-binding protein (MBP ...
-
bioRxiv - Molecular Biology 2021Quote: ... Trichoplusia ni High Five cells (Expression Systems) were infected (or coinfected ...
-
bioRxiv - Cell Biology 2023Quote: ... and Tni (High-Five) cells (Expression System). Sf9 cells were used for baculovirus preparation and maintained in Sf900 II serum free medium (Thermo Fisher) ...
-
bioRxiv - Biochemistry 2020Quote: ... A recombinant baculovirus for Gαs subunit was prepared using the BestBac™ (Expression Systems) strategy ...
-
bioRxiv - Biochemistry 2023Quote: ... Recombinant P1 baculovirus DNA was transfected into and cultured in Sf9 cells (Expression System) in penicillin-streptomycin-free ESF 921 protein-free insect cell culture media (Expression Systems ...
-
bioRxiv - Molecular Biology 2021Quote: ... and High Five™ insect cells (Expression Systems) were cultured in ESF 921 serum-free medium (Expression Systems ...
-
bioRxiv - Biochemistry 2022Quote: DHX9-His was expressed by transducing 800 ml Tni cell culture in ESF921 serum-free media (Expression Systems) at a density of 1×106 cells/ml with 16 ml baculoviral suspension (generated in Sf9 cells ...
-
bioRxiv - Biochemistry 2022Quote: ... and analysis of the recombinant bacmid were performed following the manufacturer’s instructions (ExpiSf Expression System, Gibco). ExpiSf9 cells were cultured in ExpiSf9 CD medium to a density of 5×106 cells/mL ...
-
bioRxiv - Microbiology 2023Quote: ... large-scale expression cultures were performed with Hi-5 cells cultured in the protein free ESF 921 Insect Cell Culture Medium (Expression System) and infected with 0.8-1.0% (v/v ...
-
bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen ...
-
bioRxiv - Cell Biology 2020Quote: ... Established BMEC lines were transduced with GFP-tagged RNAi lentivirus (BLOCK-iT HiPerform Lentiviral Pol II miR RNAi Expression system, Invitrogen Cat# K4934-00) targeting Raptor or Rictor ...
-
bioRxiv - Biophysics 2022Quote: ... and expressed in Tn5 (Trichoplusia ni, High Five, Expression Systems, LLC). Cell pellets resuspended in Lysis buffer (50 mM Tris pH 8.0 ...
-
bioRxiv - Biochemistry 2020Quote: ... High Five cells were grown in ESF 921 medium (Expression Systems) by shaking at 120 rpm at 27 °C until the density reached 1.5-2×106 cells mL−1 ...
-
bioRxiv - Molecular Biology 2023Quote: ... The proteins were expressed in Trichoplusia ni High Five cells (Expression Systems) by baculoviral expression ...
-
bioRxiv - Molecular Biology 2021Quote: Expression of recombinant RDR2 was optimized using the TEQC method (62) in which a 200 ml culture of Hi5 cells (Expression Systems, Inc) was infected at an estimated multiplicity of infection (eMOI ...
-
bioRxiv - Biochemistry 2021Quote: ... and High Five cells were maintained in ESF 921 Insect Cell Culture Medium (Expression System). Human embryonic kidney 293T cells (HEK-293T ...
-
bioRxiv - Biochemistry 2020Quote: ... Sf9 and High Five cells were cultured in ESF 921 medium (Expression Systems, 96-001-01) in a shaker at 140 rpm ...
-
bioRxiv - Molecular Biology 2021Quote: ... GIPR and Gs heterotrimer were co-expressed in High Five™ cells grown in ESF 921 culture medium (Expression Systems) to a density of 3.2 × 106 cells/mL ...
-
bioRxiv - Biophysics 2019Quote: ... heterotrimeric Gs was expressed in High Five™ (Trichuplusia ni) insect cells using baculoviruses generated by the BestBac method (Expression Systems). Two separate baculoviruses were used ...
-
bioRxiv - Plant Biology 2021Quote: ... Plasmid-DNA was isolated with the ZymoPURE Plasmid Midiprep (ZymoGenetics, Seattle, Washington, United States The HY5 protein was expressed with TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega ...
-
bioRxiv - Biochemistry 2022Quote: ... Omicron-SARS-CoV-2 spike RBD and ACE2 variants (CVD293 and CVD432) were expressed in high density Expi293FTM cells in Expi293TM expression media following manufacturer’s protocol (Expi293 Expression System, ThermoFisher Scientific). Briefly ...
-
bioRxiv - Plant Biology 2021Quote: ... Proteins were produced using in vitro transcription and translation system using wheat germ extract following manufactures’ instructions (SP6 High Yield Expression System from Promega). Briefly ...
-
bioRxiv - Immunology 2023Quote: ... M-IgA was expressed by transfecting a pair of alpha heavy and kappa light chain genes into FreeStyle CHO-S cells that were grown in a 24-well plate according to the manufacturer’s protocol (CHOgro High Yield Expression System, Takara Bio, Shiga, Japan). For large-scale antibody production ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... and then used to infect 1 L of Sf21 cells (Expression Systems) and incubated for 48 hrs at 27°C ...
-
bioRxiv - Immunology 2020Quote: ... one liter of cells (2.5×106 cells ml−1, medium from Expression Systems) was infected with 20 ml baculovirus at 28 ℃ ...
-
bioRxiv - Immunology 2020Quote: 50 ml of sf21 cells (2.5×106 cells ml−1, medium from Expression Systems) were infected with 1 ml baculovirus of GST-rNLRP1FIIND wild-type or mutants ...
-
bioRxiv - Biochemistry 2023Quote: Mouse CRY1 PHR domain (residues 1-491) was expressed in Sf9 suspension insect cells (Expression Systems) as previously described (Parico et al. ...
-
bioRxiv - Biophysics 2019Quote: ... 13 mL of the second amplification was used to infect 1 L of Sf21 cells (Expression Systems) at 2.8 × 106 cells/mL and incubated at 27°C for 48 hours ...
-
bioRxiv - Biophysics 2019Quote: ... 1 microgram of bacmid DNA was diluted in ESF 921 media (Expression systems, no antibiotic or serum), mixed with 6 μL of Fugene HD transfection reagent (Promega ...
-
bioRxiv - Cell Biology 2019Quote: ... elegans embryos were permeabilized using perm-1 RNAi (Carvalho et al., 2011) and dissected into a 62% solution of ESF-921 Media (Expression Systems). To arrest the embryos at metaphase ...
-
bioRxiv - Biochemistry 2021Quote: ... Sf9 cells were cultured at 27 °C in 1 L culture flasks with ESF-921 insect cell culture medium (Expression systems). Next ...
-
bioRxiv - Biochemistry 2020Quote: ... which was amplified once to give a V1 virus stock used to infect 1 l cultures of Sf9 cells (Expression Systems). Cells were grown in ESF 921 Insect Cell Culture Medium Protein Free (expression systems 96-001-01 ...