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Citations for Expression Systems :
1 - 45 of 45 citations for Recombinant Human CD40 protein Fc tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... His6-tagged human Gβ1and Gγ2 were expressed in Tni insect cells (Expression systems) using baculovirus as previously described ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... were infected with recombinant baculovirus (BestBac Expression Systems) at a density of ∼4.0 × 106 cells per ml ...
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bioRxiv - Biochemistry 2019Quote: ... or GST-tagged BMAL1 PAS-AB were expressed in Sf9 suspension insect cells (Expression Systems). Sf9 suspension cells were infected with a P3 virus at 1.2 × 106 cells per milliliter and grown for 72 hours ...
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bioRxiv - Biochemistry 2020Quote: ... Expression was performed by transducing recombinant baculoviruses into Sf21 insect cells (Expression Systems). Cells were harvested by centrifugation and resuspended in 25 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... A recombinant baculovirus (P0) was produced in SF9 cells (Expression Systems, CA, USA) as described in the protocol of the BestBactm 2.0 Δ v-cath/ChiA Baculovirus Cotransfection Kit (Expression Systems ...
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bioRxiv - Cell Biology 2021Quote: ... First passage (P1) recombinant baculovirus was generated by transfecting Sf9 cells (Expression Systems) with bacmid DNA complexed using linear polyethylenimine (PEI MAX ...
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bioRxiv - Biophysics 2023Quote: ... recombinant FUS was expressed in Sf9 insect cells (Expression Systems, Cat#94-001F) for 72 h using the baculovirus expression system87 and produced as a C-terminal SNAP fusion with an N-terminal maltose-binding protein (MBP ...
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bioRxiv - Biochemistry 2020Quote: ... A recombinant baculovirus for Gαs subunit was prepared using the BestBac™ (Expression Systems) strategy ...
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bioRxiv - Biochemistry 2023Quote: ... Recombinant P1 baculovirus DNA was transfected into and cultured in Sf9 cells (Expression System) in penicillin-streptomycin-free ESF 921 protein-free insect cell culture media (Expression Systems ...
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bioRxiv - Biophysics 2024Quote: ... The virus titer was determined by gp64-PE mouse anti-baculovirus antibody (Expression Systems, CA) using a Guava benchtop Flow Cytometer (Millipore ...
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bioRxiv - Cell Biology 2021Quote: His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...
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bioRxiv - Biochemistry 2022Quote: ... and analysis of the recombinant bacmid were performed following the manufacturer’s instructions (ExpiSf Expression System, Gibco). ExpiSf9 cells were cultured in ExpiSf9 CD medium to a density of 5×106 cells/mL ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Viral titres were analysed by flow cytometry on cells stained with gp64-PE antibody (Expression Systems). Human GLP1R ...
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bioRxiv - Cell Biology 2019Quote: ... Protein-Free (Expression Systems), supplemented with Fetal Bovine Serum (2% final concentration).
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bioRxiv - Biophysics 2022Quote: ... Protein Free (Expression Systems) and seeded in a 6-well plate to produce baculovirus ...
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bioRxiv - Biochemistry 2022Quote: ... Titers were determined by flow cytometric analysis staining P1 infected cells with gp64-PE antibody (Expression Systems). Expression of AE1 for structural studies was carried out by infection of Sf9 cells at a cell density of 2-3 ×106 cells/ml with P1 virus at MOI (multiplicity of infection ...
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bioRxiv - Cell Biology 2020Quote: ... Recombinant adenoviral vectors were produced and purified using HEK293A cells according to manufacturer’s protocol (ViraPower Adenoviral Expression System; Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Established BMEC lines were transduced with GFP-tagged RNAi lentivirus (BLOCK-iT HiPerform Lentiviral Pol II miR RNAi Expression system, Invitrogen Cat# K4934-00) targeting Raptor or Rictor ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Approximate titers were estimated by flow cytometric analysis staining P1 infected cells with gp64-PE antibody (Expression Systems). All protein for this study ...
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bioRxiv - Biophysics 2020Quote: ... 1μg of recombinant bacmid DNA was combined in 1 mL of ESF-921 media (Expression Systems, Cat. #96-001-01) with 20 μL of Cellfectin II (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: ... Viral titers were performed by the flow cytometric method23 on a Guava easycyte 8HT in which cells were stained with Anti-gp64-PE antibody (Expression Systems).
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bioRxiv - Molecular Biology 2021Quote: Expression of recombinant RDR2 was optimized using the TEQC method (62) in which a 200 ml culture of Hi5 cells (Expression Systems, Inc) was infected at an estimated multiplicity of infection (eMOI ...
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bioRxiv - Bioengineering 2020Quote: The RBD (residues 319-541) of the SARS-Cov-2 S protein was expressed as a secreted protein in Spodoptera frugiperda Sf9 cells (Expression Systems) using the Bac-to-bac baculovirus method (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... The percentage of cells infected by virus was quantified by flow cytometry following staining of 10 µL of cells with 10 µL of PE-conjugated anti-gp64 antibody (Expression Systems, catalog #97-201) for 20 min in the dark at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmid-DNA was isolated with the ZymoPURE Plasmid Midiprep (ZymoGenetics, Seattle, Washington, United States The HY5 protein was expressed with TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... and protein was expressed in Sf9 cells (Expression Systems) using the Bac-to-Bac Baculovirus expression system (Invitrogen) ...
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bioRxiv - Biophysics 2022Quote: ... The protein was expressed in Trichoplusia ni ovarian cells (Expression Systems) for 60-72 hrs maintained in ESF 921 Insect Cell Culture Medium (Expression Systems ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was expressed in the Sf9 insect cell (Expression system) in the ESF921 medium using the Bac-to-Bac baculovirus system ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were expressed in Trichoplusia ni High Five cells (Expression Systems) by baculoviral expression ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein expression was carried out in Spodoptera Frugiperda cells (Sf9, Expression Systems) using the Bac-to-Bac Baculovirus expression system (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... A single bicistronic baculovirus encoding the human Gβ1 subunit with a N-terminal 6x His-tag and rhinovirus 3C protease site and untagged human Gγ2 subunit was generated using the BestBac method (Expression systems) in Spodoptera frugiperda (Sf9 ...
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bioRxiv - Biochemistry 2023Quote: ... in penicillin-streptomycin-free ESF 921 protein-free insect cell culture media (Expression Systems) in the presence of PEI ...
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bioRxiv - Biochemistry 2024Quote: ... were cultured in protein free ESF 921 insect cell culture media (Expression Systems LLC). Bacmid preparations ...
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bioRxiv - Molecular Biology 2021Quote: HMCES-FLAG proteins were expressed in 250 mL suspension cultures of Sf9 cells (Expression Systems) by infection with baculovirus expressing rHMCES-FLAG for 72 hrs ...
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bioRxiv - Genetics 2021Quote: ... were transfected using PEI transfection methods and cultured in protein-free ESF921 media (Expression Systems, 96-001). For 6-well plate transfection ...
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bioRxiv - Biophysics 2023Quote: ... Sf9 cells were obtained from Expression Systems and maintained in protein-free cell culture media purchased from Expression Systems (Cat. no: 96-001-01) at 27°C with 135 rpm shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi5 cells grown in ESF-921 media (Expression Systems, Davis, CA, United States) at 27 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were maintained in protein-free cell culture media purchased from Expression Systems (Cat. no: 96-001-01) at 27°C with 135 rpm shaking ...
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bioRxiv - Biochemistry 2021Quote: ... Protein expression was induced by baculovirus infection of 6-8 liter cultures of Sf9 cells in ESF921 medium (Expression Systems) at a density of ~2 × 106 cells/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... were seeded per well in a 6-well plate in protein-free ESF 921 media (Expression Systems Cat# 96-001) with no additives or antibiotics ...
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bioRxiv - Cell Biology 2023Quote: ... Tni cells were used for large-scale protein expression and are maintained in ESF 921 serum free medium (Expression Systems).
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bioRxiv - Cell Biology 2023Quote: For expression of Nv-Osk and Nv-Vas proteins in S2 (Drosophila Schneider 2) cells (Expression Systems, cat # 94-005S), corresponding cDNAs were subcloned under control of Actin5C promoter (GenScript) ...
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bioRxiv - Immunology 2021Quote: ... The protein was expressed in 2 L of Sf9 cells at 2×106 cells/mL maintained in ESF921 media (Expression Systems) by adding 25 mL virus per liter of culture ...
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bioRxiv - Microbiology 2023Quote: ... large-scale expression cultures were performed with Hi-5 cells cultured in the protein free ESF 921 Insect Cell Culture Medium (Expression System) and infected with 0.8-1.0% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... with a multiplicity of infection of 5 of baculovirus at 1×106 cells per ml and incubated at 27°C for 70 hours in Protein-free Insect Cell Culture Medium (Expression Systems, 96-001-01) with penicillin/streptomycin ...