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Citations for Expression Systems :
1 - 42 of 42 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade E CM244 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The virus titers were measured by flow cytometry assay (Expression Systems).
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bioRxiv - Biochemistry 2020Quote: ... which was amplified once to give a V1 virus stock used to infect 1 l cultures of Sf9 cells (Expression Systems). Cells were grown in ESF 921 Insect Cell Culture Medium Protein Free (expression systems 96-001-01 ...
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bioRxiv - Biophysics 2021Quote: ... and the virus was prepared using the BestBac system (Expression System, LLC). N-terminal Flag-tagged human Gβ1 ...
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bioRxiv - Biophysics 2024Quote: ... The virus titer was determined by gp64-PE mouse anti-baculovirus antibody (Expression Systems, CA) using a Guava benchtop Flow Cytometer (Millipore ...
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bioRxiv - Biophysics 2022Quote: ... P0 virus was produced by transfection with FuGENE HD and BestBac 1.0 Linearized Baculovirus DNA (Expression Systems) followed by a viral amplification to P1 ...
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bioRxiv - Biophysics 2023Quote: ... and the P3 virus was used to infect Tni suspension insect cell culture (Expression Systems; Cat. 94-002S) for protein expression.
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were infected (multiplicity of infection of 6 for each virus) with separate baculoviruses (prepared using the Bac-to-Bac Baculovirus Expression System, Invitrogen) containing the CXCL12LRHQ gene as well as the ACKR3 gene (residues 2-362 ...
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bioRxiv - Cell Biology 2019Quote: ... Protein-Free (Expression Systems), supplemented with Fetal Bovine Serum (2% final concentration).
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bioRxiv - Biophysics 2022Quote: ... Protein Free (Expression Systems) and seeded in a 6-well plate to produce baculovirus ...
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bioRxiv - Biochemistry 2021Quote: ... His6-tagged human Gβ1and Gγ2 were expressed in Tni insect cells (Expression systems) using baculovirus as previously described ...
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bioRxiv - Bioengineering 2020Quote: The RBD (residues 319-541) of the SARS-Cov-2 S protein was expressed as a secreted protein in Spodoptera frugiperda Sf9 cells (Expression Systems) using the Bac-to-bac baculovirus method (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmid-DNA was isolated with the ZymoPURE Plasmid Midiprep (ZymoGenetics, Seattle, Washington, United States The HY5 protein was expressed with TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... and protein was expressed in Sf9 cells (Expression Systems) using the Bac-to-Bac Baculovirus expression system (Invitrogen) ...
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bioRxiv - Biophysics 2022Quote: ... The protein was expressed in Trichoplusia ni ovarian cells (Expression Systems) for 60-72 hrs maintained in ESF 921 Insect Cell Culture Medium (Expression Systems ...
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bioRxiv - Biochemistry 2023Quote: ... The protein was expressed in the Sf9 insect cell (Expression system) in the ESF921 medium using the Bac-to-Bac baculovirus system ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were expressed in Trichoplusia ni High Five cells (Expression Systems) by baculoviral expression ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Protein expression was carried out in Spodoptera Frugiperda cells (Sf9, Expression Systems) using the Bac-to-Bac Baculovirus expression system (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... A single bicistronic baculovirus encoding the human Gβ1 subunit with a N-terminal 6x His-tag and rhinovirus 3C protease site and untagged human Gγ2 subunit was generated using the BestBac method (Expression systems) in Spodoptera frugiperda (Sf9 ...
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bioRxiv - Biochemistry 2023Quote: ... in penicillin-streptomycin-free ESF 921 protein-free insect cell culture media (Expression Systems) in the presence of PEI ...
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bioRxiv - Biochemistry 2024Quote: ... were cultured in protein free ESF 921 insect cell culture media (Expression Systems LLC). Bacmid preparations ...
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bioRxiv - Molecular Biology 2021Quote: HMCES-FLAG proteins were expressed in 250 mL suspension cultures of Sf9 cells (Expression Systems) by infection with baculovirus expressing rHMCES-FLAG for 72 hrs ...
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bioRxiv - Genetics 2021Quote: ... were transfected using PEI transfection methods and cultured in protein-free ESF921 media (Expression Systems, 96-001). For 6-well plate transfection ...
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bioRxiv - Biophysics 2023Quote: ... Sf9 cells were obtained from Expression Systems and maintained in protein-free cell culture media purchased from Expression Systems (Cat. no: 96-001-01) at 27°C with 135 rpm shaking ...
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bioRxiv - Molecular Biology 2019Quote: ... All proteins were expressed in Hi5 cells grown in ESF-921 media (Expression Systems, Davis, CA, United States) at 27 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were maintained in protein-free cell culture media purchased from Expression Systems (Cat. no: 96-001-01) at 27°C with 135 rpm shaking ...
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bioRxiv - Biochemistry 2021Quote: ... Protein expression was induced by baculovirus infection of 6-8 liter cultures of Sf9 cells in ESF921 medium (Expression Systems) at a density of ~2 × 106 cells/ml ...
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bioRxiv - Molecular Biology 2020Quote: ... were seeded per well in a 6-well plate in protein-free ESF 921 media (Expression Systems Cat# 96-001) with no additives or antibiotics ...
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bioRxiv - Cell Biology 2023Quote: ... Tni cells were used for large-scale protein expression and are maintained in ESF 921 serum free medium (Expression Systems).
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bioRxiv - Cell Biology 2023Quote: For expression of Nv-Osk and Nv-Vas proteins in S2 (Drosophila Schneider 2) cells (Expression Systems, cat # 94-005S), corresponding cDNAs were subcloned under control of Actin5C promoter (GenScript) ...
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bioRxiv - Immunology 2021Quote: ... The protein was expressed in 2 L of Sf9 cells at 2×106 cells/mL maintained in ESF921 media (Expression Systems) by adding 25 mL virus per liter of culture ...
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bioRxiv - Microbiology 2023Quote: ... large-scale expression cultures were performed with Hi-5 cells cultured in the protein free ESF 921 Insect Cell Culture Medium (Expression System) and infected with 0.8-1.0% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... with a multiplicity of infection of 5 of baculovirus at 1×106 cells per ml and incubated at 27°C for 70 hours in Protein-free Insect Cell Culture Medium (Expression Systems, 96-001-01) with penicillin/streptomycin ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and then used to infect 1 L of Sf21 cells (Expression Systems) and incubated for 48 hrs at 27°C ...
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bioRxiv - Immunology 2020Quote: ... one liter of cells (2.5×106 cells ml−1, medium from Expression Systems) was infected with 20 ml baculovirus at 28 ℃ ...
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bioRxiv - Immunology 2020Quote: 50 ml of sf21 cells (2.5×106 cells ml−1, medium from Expression Systems) were infected with 1 ml baculovirus of GST-rNLRP1FIIND wild-type or mutants ...
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bioRxiv - Biochemistry 2023Quote: Mouse CRY1 PHR domain (residues 1-491) was expressed in Sf9 suspension insect cells (Expression Systems) as previously described (Parico et al. ...
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bioRxiv - Biophysics 2019Quote: ... 13 mL of the second amplification was used to infect 1 L of Sf21 cells (Expression Systems) at 2.8 × 106 cells/mL and incubated at 27°C for 48 hours ...
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bioRxiv - Biophysics 2019Quote: ... 1 microgram of bacmid DNA was diluted in ESF 921 media (Expression systems, no antibiotic or serum), mixed with 6 μL of Fugene HD transfection reagent (Promega ...
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bioRxiv - Biophysics 2020Quote: ... 1μg of recombinant bacmid DNA was combined in 1 mL of ESF-921 media (Expression Systems, Cat. #96-001-01) with 20 μL of Cellfectin II (Thermo Fisher ...
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bioRxiv - Cell Biology 2019Quote: ... elegans embryos were permeabilized using perm-1 RNAi (Carvalho et al., 2011) and dissected into a 62% solution of ESF-921 Media (Expression Systems). To arrest the embryos at metaphase ...
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bioRxiv - Biochemistry 2021Quote: ... Sf9 cells were cultured at 27 °C in 1 L culture flasks with ESF-921 insect cell culture medium (Expression systems). Next ...
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bioRxiv - Cell Biology 2021Quote: His-tagged mouse CRY2 D325H PHR domain (residues 1-512) was expressed in Sf9 suspension insect cells (Expression Systems, Davis, CA) infected with a P3 baculovirus at 1.2 × 106 cells per milliliter and grown for 72 hours at 27°C with gentle shaking ...