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Citations for Molecular Dimensions :
1 - 35 of 35 citations for Recombinant Human EIF4EBP1 Protein His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 μl of protein solution and 2 μl of crystallization mother liquor at 18°C ...
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bioRxiv - Microbiology 2019Quote: Purified recombinant BepA was used with proprietary crystal screens (supplied by Molecular Dimensions and Jena Bioscience) in sitting drop crystallization experiments using 2 µl of protein solution and 2 µl of crystallization mother liquor at 18°C ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 7.0 (The Protein Complex Suite F1, Molecular Dimensions, protein: precipitant ratio 2:1). The crystal was harvested and flash-cooled without adding cryo-protectant ...
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bioRxiv - Neuroscience 2020Quote: ... The protein crystallized in Wizard 1 (Molecular Dimensions), condition #1 ...
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bioRxiv - Biochemistry 2020Quote: SeMet labeled protein was expressed using Selenomet media from Molecular Dimensions. The cultures were initiated from a frozen stock into Selenomet media supplemented with 50 µg/ml of L-methionine and 100 µg/ml carbenicillin ...
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bioRxiv - Biophysics 2021Quote: Human malonate-bound SR was crystallised at 20 °C in 48-well MRC Maxi sittingdrop plates (Molecular Dimensions) in 25% PEG 3350 ...
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bioRxiv - Biophysics 2021Quote: ... the protein was subjected to the PACT crystallization screen (Molecular Dimensions, USA), with hits identified in several conditions for both inhibitors ...
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bioRxiv - Developmental Biology 2019Quote: ... japonica protein was expressed in SelenoMethionine Medium Complete (Molecular Dimensions, Suffolk, UK), and grown ...
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bioRxiv - Biochemistry 2024Quote: ... Selenomethionine substituted protein was produced using a premade SelenoMet expression medium (Molecular Dimensions). Cells were grown for 16h in 100 ml Minimal Media starter cultures containing 40 μg/ml methionine ...
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bioRxiv - Biophysics 2021Quote: ... the protein solution was homogenized with 1.5-folds volumes molten 9.9 MAG (Molecular Dimensions, USA) using a gas-tight coupled syringe device (Hampton Research ...
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bioRxiv - Molecular Biology 2020Quote: ... Buffer exchanged proteins were screened for conditions that produced crystals using the NR LBD (Molecular Dimensions), Structure (Molecular Dimensions) ...
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bioRxiv - Biophysics 2020Quote: ... Selenomethionine labeled ORF8 double mutant protein was expressed using SelenoMet Medium Base according to manufacturer’s instructions (Molecular dimensions). Purification was carried out in the same manner as wild-type but the TEV-cleavage step was omitted.
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bioRxiv - Biochemistry 2022Quote: ... The protein in buffer C (2.2 mg/ml) was crystallized using Ligand-Friendly Screening HT-96 (Molecular Dimensions) for the initial screening and protein crystals grew in 0.1 M Bis-Tris Propane pH 6.5 ...
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bioRxiv - Biochemistry 2020Quote: Initial protein crystals were grown at 4°C in condition E10 of the MemGold2-ECO Screen (Molecular Dimensions; 0.05 M Na-acetate pH 4.5 ...
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bioRxiv - Microbiology 2022Quote: ... Nucleotide-free Irgb6-T95D crystals diffracting to 2.05 Å resolution were obtained from sitting drops with a 0.5 μl of protein solution and a 0.5 μl of reservoir solution consisting of 0.2M Sodium malonate pH7.0 (Molecular Dimensions), 20% Polyethylene Glycol 3350 (Molecular Dimensions ...
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bioRxiv - Molecular Biology 2023Quote: ... crystallization trials performed at 287 K with 6.6 mg/ml protein yielded crystals in BCS screen (Molecular dimensions) condition D9 and optimized to 17.27% Tacsimate pH 6.34,10% Ethylene Glycol ...
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bioRxiv - Bioengineering 2021Quote: ... Crystallization experiments were set up in sitting drop geometry with 0.5 µl protein sample equilibrating with 0.5 µl reservoir from screen kits (Hampton and Molecular Dimensions) at room temperature ...
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bioRxiv - Microbiology 2021Quote: Nucleotide-free Irgb6 crystals diffracting to 1.9 Å resolution were obtained from sitting drops with a 12 mg/ml protein solution and a reservoir solution consisting of 0.1 M MIB buffer pH 6.0 (Molecular Dimensions), 25% Polyethylene Glycol 1500 (Molecular Dimensions ...
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bioRxiv - Biochemistry 2020Quote: ... This preparation of the protein was mixed 1 μL:1 μL with the Morpheus HT screen reagents (Molecular Dimensions) in a 96-well SwissSci MRC UV-transmissible sitting drop plate ...
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bioRxiv - Plant Biology 2021Quote: ... Drops composed of 0.3 μL of protein solution and 0.3 μL of reservoir solution were set up in MRC 96-well crystallisation plates (Molecular Dimensions), which were dispensed using an Oryx Nano or an Oryx8 robot (Douglas Instruments) ...
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bioRxiv - Plant Biology 2020Quote: ... 0.3 μl of 11 mg/ml protein was combined with 0.3 μl reservoir solution from the commercial Morpheus screen (Molecular Dimensions). Crystals appeared in several conditions ...
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bioRxiv - Biochemistry 2022Quote: ... 200 nl of protein was added to 200 nl of precipitant containing 32% low MW PEG smear solution (Molecular Dimensions) and 100 mM Tris pH 8.5 ...
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bioRxiv - Plant Biology 2023Quote: ... Drops composed of 0.3 μL of protein solution and 0.3 μL of reservoir solution were set up in MRC 96-well crystallisation plates (Molecular Dimensions), which were dispensed using an Oryx Nano or an Oryx8 robot (Douglas Instruments) ...
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bioRxiv - Biochemistry 2022Quote: ... to set up dual droplets for each condition with drop volume of 0.2 and 0.3 µl (1:1 and 2:1, protein vs reservoir solution) on 96-well sitting drop crystallization plates (Molecular Dimensions). The crystal used for final data collection was grown from a manually set up droplet containing 2 µl of protein and 1 µl of reservoir solution ...
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bioRxiv - Cell Biology 2019Quote: ... The crystals were obtained using the sitting-drop vapor-diffusion method at 18°C by mixing 300 nl of protein-ligand complex with a reservoir solution (Morpheus screen; Molecular dimensions). The diffraction data set was collected at BESSY-II beamline (Mueller et al. ...
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bioRxiv - Biochemistry 2020Quote: ... were obtained at a protein concentration of 8 mg/ml in the conditions “B11” from the Morpheus II crystallisation screen (Molecular Dimensions): 2 mM divalents mix (0.5 mM MnCl3 ...
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bioRxiv - Biochemistry 2019Quote: ... Droplets of 0.1 µL protein solution at 10 mg mL−1 were mixed with an equal volume of reservoir solution using screens from Molecular Dimensions (PACT ...
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bioRxiv - Microbiology 2020Quote: ... 100 and 200 nL drops of protein and 100 nL of screen solution were dispensed into 96 well MRC crystallization plates (Molecular Dimensions) by a Mosquito (TTP Labtech ...
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bioRxiv - Microbiology 2022Quote: Initial crystallisation trials were performed in 96-well plates using sitting drops composed of 200 nL protein plus 200 nL reservoir solution Initial crystals were identified in broad matrix screens (PACT PREMIER, Molecular Dimensions) and diffraction-quality crystals of Trag25_CTD grew in 0.1 M SPG ...
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bioRxiv - Microbiology 2023Quote: Crystallization conditions for falcilysin were screened with a protein concentration of 22 mg⋅ml-1 using commercial kits (JCSG-plusTM & Morpheus from Molecular Dimensions; Index & PEG/Ion ScreenTM from Hampton Research ...
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bioRxiv - Biochemistry 2024Quote: ... 0.4 µL drops were produced mixing an equal volume of protein and precipitant solutions (PACT, LMB and JCSG-plus crystallization kits; Molecular Dimensions) by an Oryx8 dispensing robot (Douglas Instruments ...
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bioRxiv - Biophysics 2021Quote: Initial crystallisation hits for the 31-145 DM CtIP protein were observed with condition F6 of the Morpheus® commercial screen MD-47 (Molecular Dimensions). Crystals were improved by hanging-drop vapor diffusion and micro-seeding ...
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bioRxiv - Biochemistry 2023Quote: ... *JbCDTCM crystals were obtained at 20 °C from a 1:1 (200 nL:200 nL) mixture of protein sample and solution C1 from the Morpheus crystallization screening kit (Molecular Dimensions Ltd.), containing 30% w/v PEG500MME_P20K (10% w/v PEG 20000 ...
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bioRxiv - Biochemistry 2021Quote: ... The CodB protein with 1mM cytosine was mixed with monoolein at 60:40 (w/w) ratio using a coupled syringe device (Molecular Dimensions Ltd UK) and crystallisation trials were set up at 20°C using glass sandwich plates using a Mosquito Robot ...
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bioRxiv - Molecular Biology 2024Quote: ... The crystal for structural determination of HSPB7ACD C131S was discovered and harvested after setting up for 10 days from a well containing 0.2 M magnesium formate dihydrate and 20% w/v polyethylene glycol (PEG) 3350 (JCSG-plus A5, Molecular Dimensions, protein: precipitant ratio 2:1). Crystals were cryoprotected using 10% glycerol in well solution ...