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Citations for Electron Microscopy Sciences :
1 - 40 of 40 citations for Inactive Serine Protease 35 PRSS35 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... 35S::PILS5-GFP (PILS5OE) seedlings descended from 3000 ethyl methanesulfonate (EMS) (0.3% ...
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bioRxiv - Bioengineering 2019Quote: Samples were fixed for 35 min in 4% paraformaldehyde (EMS, Cat# 15710), washed three times with 1× PBS ...
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bioRxiv - Synthetic Biology 2019Quote: Cells were passed through a 35 μm nylon strainer (EMS #64750-25). Green fluorescent signal from GFP and red fluorescent signal from mCherry were detected on a BD Accuri C6 flow cytometer (675 nm LP filter ...
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bioRxiv - Developmental Biology 2020Quote: ... immersed in 0.8% low-melting point agarose and mounted in glass-bottomed 35 mm Petri dishes (Electron Microscopy Sciences). After mounting ...
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bioRxiv - Neuroscience 2021Quote: ... immersed in 0.8% low-melting point agarose and mounted in glass-bottomed 35 mm Petri dishes (Electron Microscopy Sciences). After mounting ...
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bioRxiv - Cell Biology 2023Quote: ... high Grid-50 Glass Bottom is a 35 mm then fixed in Karnovsky’s fixative: 2% Glutaraldehyde (EMS Cat# 16000) and 4% pFormaldehyde (EMS Cat# 15700 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells are dehydrated in series of ethanol (e.g., 35%, 50%, 75%, 95%, and 100%) are washed three changes in pure epoxy resin (Electron Microscopy Sciences) and infiltrated overnight ...
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bioRxiv - Cell Biology 2023Quote: ... was inserted into the left ventricle to transcardially perfuse the animal with 20 ml of PBS followed by 1.5 ml/g (−35 ml) of 4% (v/v) paraformaldehyde (PFA) (15710, Electron Microscopy Sciences). Note that the transcardial perfusion of 4% PFA is critical to preserve the sample to visualize primary cilia in tissues ...
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bioRxiv - Molecular Biology 2021Quote: Cells grown on glass bottom dishes (35 mm, 14 mm glass, uncoated, Mat-Tek Corporation) were fixed with 4% Paraformaldehyde (Electron Microscopy Sciences) in 1 M HEPES (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: The selected MC12 cell lines were plated on a 35-mm glass bottom dish (IWAKI) and fixed with 4% formaldehyde (Electron Microscopy Sciences) in 250 mM HEPES-NaOH (pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: Mice were anesthetized and perfused with normal Ringer’s solution at 35°C followed by 0.15M cacodylate buffer containing 4% formaldehyde (Electron Microscopy Sciences, Hartfield, PA). Brain tissue was cut into 100 μm vibratome sections ...
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bioRxiv - Biochemistry 2019Quote: ... 3 L of the αSyn sample solution of interest (35 M, directly from the fibrillation assay) was pipetted on a discharged carbon-coated copper TEM grid (Electron Microscopy Sciences, CF400CU; 400 mesh) and incubated for 1 min ...
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bioRxiv - Cell Biology 2020Quote: ... sections of 160 nm thickness were cut with a 35° DiATOME ultra knife and collected on carbon-coated formvar finder grids (Ted Pella 01910-F; Electron Microscopy Sciences, Hatfield, PA, USA). Grids were kept in the dark throughout the whole process and were observed as soon as possible ...
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bioRxiv - Neuroscience 2023Quote: ... 15nm or 18nm gold secondary antibodies (EMS). Grids were washed ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-mouse IgG/IgM-6nm gold secondary antibody (EMS) diluted 1:25 in 1% BSA PBS was added for 1.5 hours followed by another washing step ...
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bioRxiv - Cell Biology 2019Quote: ... incubated for 45 minutes with goat anti-rabbit 15nm gold antibody (EMS) diluted 1:20 in filtered blocking solution ...
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bioRxiv - Neuroscience 2020Quote: ... Girds were immersed in 15µl beads of secondary antibody (Electron microscopy sciences, goat-anti-rabbit 15 nm ...
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bioRxiv - Cell Biology 2019Quote: ... goat secondary antibodies conjugated with 6 nm and 10 nm gold particles (EMS) were applied to the sections on the grids ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used were 6 nm anti-mouse immunogold particles (Electron Microscopy Sciences). Negative staining was carried out as previously described [17] ...
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bioRxiv - Cell Biology 2023Quote: ... and incubated with anti-rabbit secondary antibody conjugated to gold particles (Electron Microscopy Sciences) in the blocking solution for 1 h ...
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bioRxiv - Genetics 2019Quote: ... for 20 minutes (for all antibodies except GluRIIA) or in Bouin’s Fixative (Electron Microscopy Sciences) for 5 minutes (for GluRIIA staining) ...
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bioRxiv - Neuroscience 2024Quote: ... A secondary antibody (goat anti-mouse IgG conjugated to 15 nm colloidal gold; Electron Microscopy Sciences) diluted 1:20 in 0.1% BSA in PBS was added for 1.5 h ...
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bioRxiv - Microbiology 2022Quote: ... Secondary antibodies used were goat-anti-Rabbit IgG conjugated to 10nm gold particles (Electron Microscopy Sciences, Cat. # 25109). We also labeled the samples with secondary gold-conjugated antibody alone to test for the non-specific labeling and found virtually no labeling in each case (data not shown) ...
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bioRxiv - Cell Biology 2019Quote: ... All reagents for electron microscopy and secondary antibodies for immuno-gold labelling were purchased from Electron Microscopy Sciences.
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bioRxiv - Cancer Biology 2019Quote: ... and surface antibodies were fixed in place by a 15-minute incubation with 1.5% paraformaldehyde (PFA; Electron Microscopy Sciences). Subsequently ...
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bioRxiv - Molecular Biology 2019Quote: ... and labeled for 1 h with 20 µg/mL anti-PEG10 antibody diluted in EM blocking solution (EMS, Aurion). Samples were washed in PBS for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... segments were rinsed thoroughly with PBS and incubated overnight with the secondary antibody Ultra small (0.8 nm) Gold conjugated (EMS) in blocking buffer at RT ...
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bioRxiv - Cell Biology 2024Quote: ... segments were rinsed thoroughly with PBS and incubated overnight with the secondary antibody Ultra small (0.8 nm) Gold conjugated (EMS) in blocking buffer at RT ...
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bioRxiv - Cell Biology 2019Quote: The following reagents/primary antibodies were used for immunofluorescence analyses: paraformaldehyde solution (10%) was purchased from Electron Microscopy Sciences (Hatfield, PA); mouse anti-TfR2 ...
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bioRxiv - Cell Biology 2021Quote: Secondary antibodies: donkey anti-rabbit IgG (H&L) conjugated with 25-nm gold particles (Electron Microscopy Science Aurion #25708, PA, USA), donkey anti-mouse IgG (H&L ...
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bioRxiv - Neuroscience 2022Quote: ... and subsequently incubated with a colloidal gold-conjugated secondary antibody (1:50; goat anti-chicken IgG gold UltraSmall, Electron Microscopy Sciences) for 24 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... The grids were blocked again for 1 h at room temperature and incubated for 2 h with 1:20 Goat-Anti-Rabbit IgG antibodies (H+L) (EMS 25104) and 1:10 Goat-Anti-Mouse IgG (H+L ...
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bioRxiv - Developmental Biology 2024Quote: Larvae were dissected to optimally visualize the body wall muscles in ice-cold HL3.1 buffer and fixed with 4% paraformaldehyde in HL3.1 for 20 minutes for all antibodies except GluRIIA for which samples were fixed in Bouin’s fixative (Electron Microscopy Sciences, 15990) for 5 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with a secondary antibody conjugated with electron-dense colloidal gold nanoparticles of 10 nm size (Aurion, Electron Microscopy Sciences). GFP (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... The grids were washed twice with blocking buffer before labeling with secondary antibodies (goat anti-rat or goat anti-rabbit, 10nm, Electron Microscopy Sciences), rinsed three times in 1X PBS ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Cell Biology 2023Quote: ... labeled with a secondary anti-rabbit or anti-mouse gold (depending on the primary antibody used) at RT for 1 hour (1:200 goat-anti-rabbit or goat-anti-mouse, Electron Microscopy Sciences), washed in 1% PBS with 3 sequential 50 µL drops ...
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bioRxiv - Pathology 2022Quote: ... Slides were incubated at RT for an hour in secondary antibody and mounted with Fluoro-Gel II containing DAPI (Electron Microscopy Sciences, 17985). Slides were viewed using a Zeiss Axio Imager.A2 Microscope and images were taken using Axiocam 105 color (chromogenic detection ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3x wash in PBS for 20 min and 1 h incubation with a secondary gold-conjugated antibody (anti-rabbit IgG 10 nm gold; EMS Electron Microscopy Sciences). After washing with PBS ...
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bioRxiv - Microbiology 2023Quote: ... the grid was floated on a 20 µl drop of anti-rabbit secondary antibody coupled to 6 nm colloidal gold particles (Electron Microscopy Sciences #25104) diluted 1:50 in blocking buffer for 1 h in a closed ...