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Citations for Gold Biotechnology :
1 - 23 of 23 citations for Recombinant E. coli LT IIa His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli were 50 µg/ml kanamycin (GoldBio) or 100 µg/ml carbenicillin (GoldBio) ...
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bioRxiv - Microbiology 2022Quote: ... coli: 200 μM isopropyl beta-D-1-thiogalactopyranoside (IPTG, GoldBio), 0.1% L-arabinose (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... All recombinant human cytokines were purchased from GoldBio (Massachusetts, USA). Following stimulation ...
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bioRxiv - Cancer Biology 2020Quote: Human recombinant IL-1α (GoldBio, St. Louis, MO; 1110-01A-100), IL-1β (GoldBio ...
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bioRxiv - Cancer Biology 2019Quote: Human recombinant IL-1α (GoldBio, St Louis MO; 1110-01A-100) and IL-1β (GoldBio ...
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bioRxiv - Bioengineering 2024Quote: ... at 1 mg/mL and elastase at 6 U/mL (E-240, Goldbio) for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine-tagged proteins were affinity purified from soluble lysate using nickel agarose beads (Gold Biotechnology). Bound protein was washed with 50 mL lysis buffer before elution using 5 mL of elution buffer containing 25 mM Tris HCl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... coli in the presence of 100 μg/mL ampicillin (GoldBio, Olivette, MO). The cultures were incubated at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... coli and isolated using nickel agarose beads (Goldbio, Cat# H-350-5). 5 μM of HaBlap was mixed with 50 μM nitrocefin in 5 mM phosphate buffer pH 7 ...
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bioRxiv - Microbiology 2020Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The His6-tagged protein variants present in the supernatant were purified using Ni2+-NTA affinity chromatography (Goldbio) and dialyzed overnight at 4 ⁰C against 50 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Microbiology 2019Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (ZymoResearch T3001) cells and plated on selection media (Ampicillin, Goldbio 69-52-3). Sanger sequencing confirmed plasmids that contained the correct protease insert ...
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bioRxiv - Molecular Biology 2023Quote: ... coli (ZymoResearch T3001) cells and plated on selection media (Ampicillin, Goldbio 69-52-3). A green-white screen46 was used to inoculate assembled colonies in selection liquid media ...
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bioRxiv - Molecular Biology 2021Quote: ... expression of recombinant protein was induced with 0.5 mM isopropyl β-D-thiogalactoside (GoldBio). For purification of PABPC1 and ePAB ...
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bioRxiv - Biochemistry 2020Quote: ... recombinants were selected on YPD agar plates containing 100 μg/ml nourseothricin (Gold Biotechnology), resulting in ySI42 ...
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bioRxiv - Immunology 2020Quote: ... Recombinant human interleukin-15 (IL-15) and Fibroblast Growth Factor-basic (bFGF) were purchased from GoldBio. Recombinant Human RANTES (CCL5 ...
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bioRxiv - Microbiology 2021Quote: ... coli TOP10 and plated on LB agar plates supplemented with 100 μg/mL hygromycin B (GoldBio, USA). An individual colony was picked and transferred to 5 mL of LB medium supplemented with 100 μg/mL hygromycin B and incubated overnight at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... Clarified cell supernatant containing recombinant antibodies was passed over Protein A Agarose (Goldbio Cat# P-400-5), followed by extensive washing with PBS ...
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bioRxiv - Biochemistry 2022Quote: ... 20 mM KCl and home-made protease inhibitor cocktail (500 μM AEBSF, 1 μM E-64, 1 μM Leupeptin, 150 nM Aprotinin) (Gold Biotechnology). Total cellular membranes were harvested by ultracentrifugation ...
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bioRxiv - Microbiology 2023Quote: ... coli strains were inoculated from an agar colony plate into 300 mL BHI media with 100 ug/mL carbenicillin (GoldBio, 00901C103) and 100 uM isopropyl β-d-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli S17-1 λ pir was routinely grown in Luria Bertani (LB) broth (LabExpress) supplemented with 100 μg/mL carbenicillin (Gold Biotechnology) for plasmid selection.