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Citations for Gold Biotechnology :
1 - 26 of 26 citations for Nickel oxalate dihydrate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... A Nickel-Nitrilotriacetic acid (Ni-NTA, GOLDBIO) column was washed with water and equilibrated in Buffer A containing 20 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... 10 ml of Nickel NTA Agarose Beads (Gold Biotechnology) was added to each clarified lysate ...
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bioRxiv - Biochemistry 2022Quote: ... soluble fractions were subjected to nickel affinity purification (GoldBio) after being exchanged into native binding buffer (20 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2020Quote: ... Cleared lysate was added to 200 μL of Nickel beads (Gold Biotech) in a small drip column (BioRad) ...
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bioRxiv - Biochemistry 2023Quote: ... coli and isolated using nickel agarose beads (Goldbio, Cat# H-350-5). 5 μM of HaBlap was mixed with 50 μM nitrocefin in 5 mM phosphate buffer pH 7 ...
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bioRxiv - Synthetic Biology 2023Quote: ... purified using Nickel NTA agarose beads (Catalog No. H-350-5, GoldBio), and eluted (500 mM NaCl ...
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bioRxiv - Molecular Biology 2022Quote: ... which was then incubated with 1.5 mL washed and packed nickel resin (GoldBio) at 4°C for one hour with gentle rocking ...
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bioRxiv - Microbiology 2024Quote: ... The supernatants were applied to a 2 ml nickel agarose (high density, GoldBio) for binding for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... which was then incubated with 1.5 mL washed and packed nickel resin (GoldBio) at 4°C for one hour with gentle rocking ...
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bioRxiv - Microbiology 2024Quote: ... and the supernatant flowed over high-density nickel agarose beads (GoldBio H-320). After washing with Buffer A and eluting with 500mM imidazole ...
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bioRxiv - Microbiology 2024Quote: ... Clarified lysates were loaded to an equilibrated column of Nickel-NTA resin (Goldbio), washed with 10-15 column volumes of wash buffer (50 mM HEPES ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was run through a nickel-NTA agarose affinity chromatography gravity column (Goldbio) equilibrated by lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... Lysates were clarified using centrifugation and proteins were purified via nickel agarose beads (Gold Biotechnology), eluted with 300mM imidazole ...
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bioRxiv - Cancer Biology 2021Quote: ... The supernatant was rotated over Nickel NTA Agarose beads (Gold Biotechnology, Cat# H-350-25) in a column (Gold Biotechnology ...
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bioRxiv - Biochemistry 2023Quote: ... Nickel-NTA Agarose resin and n-Dodecyl-β-D-Maltopyranoside (DDM) were from Gold Biotechnology. n-Decyl-β-D-Maltopyranoside (DM) ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate supernatant was then passed over a nickel-NTA affinity gravity flow column (GoldBio) pre-prepared in lysis buffer ...
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A nepenthesin insert allosterically controls catalysis in the malaria parasite protease plasmepsin VbioRxiv - Microbiology 2022Quote: ... supernatants were thawed and bound to a column of nickel agarose beads (GoldBio; St. Louis, MO), washed five times in wash buffer (20 mM imidazole ...
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bioRxiv - Biochemistry 2020Quote: ... The hexahistidine-tagged proteins were affinity purified from soluble lysate using nickel agarose beads (Gold Biotechnology). Bound protein was washed with 50 mL lysis buffer before elution using 5 mL of elution buffer containing 25 mM Tris HCl pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was added to a wash buffer-equilibrated nickel-NTA affinity gravity flow column (GoldBio) and elution of the protein was achieved using 1 column volume of elution buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2020Quote: ... The hexahistidine-tagged DXR protein was affinity purified from soluble lysate via nickel agarose beads (Gold Biotechnology). Bound protein was eluted in 300 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... Parasite material was then pelleted at 17,000 g and the supernatant poured onto nickel HTC agarose beads (Gold Biotechnology) that had been equilibrated in PBS + 100 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... The resulting supernatant was filtered through a 0.45-μm PES membrane and loaded onto pre-equilibrated Nickel NTA Agarose Beads (Goldbio). The beads were washed with equilibration/wash buffer containing 20 mM Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... The lysate was cleared by centrifugation at 16,000 rpm for 30 minutes and the supernatant passed over a nickel NTA gravity column (Goldbio) followed by washing with 50 column volumes of chilled lysis including PMSF ...
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bioRxiv - Microbiology 2021Quote: ... Lysate was centrifuged at 18,000xg for 1 hr and the supernatant containing soluble protein was bound to nickel agarose beads (Gold Biotechnology), washed with buffer containing 25mM Tris HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2022Quote: ... FN3-AFF sensors were purified from the soluble fractions of cell lysates using nickel nitriloacetate resin (Gold Biotechnology, St. Louis, MO) following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2024Quote: ... Extract was cleared by centrifugation at 12,000 rpm for 30 min prior to 2h incubation with nickel affinity beads for Ubp2 purification (GOLDBIO catalog H-355) or glutathione-containing beads (GOLDBIO G-250-5 ...