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Citations for Gold Biotechnology :
1 - 47 of 47 citations for Mouse Cytochrome C Oxidase Subunit II COX2 MT CO2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... β subunit overexpression was induced by adding IPTG (Gold Biotechnology) to 1 mM and cell growth was monitored until apparent OD600 reached 0.9 ...
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bioRxiv - Genomics 2022Quote: ... or 50 µL of Co2+-NTA beads (GoldBio). Binding was done in 50 mM Tris buffer pH 8.2 ...
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bioRxiv - Biophysics 2023Quote: ... The lysate was then loaded onto Co2+-charged TALON resin (GoldBio), equilibrated previously with the wash buffer (50 mM Tris-HCl pH 8.5 ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently run over a Co2+ agarose bead (GoldBio, H-310-25) column at 1 mL/minute ...
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bioRxiv - Cell Biology 2021Quote: ... Lysate was run over a Co2+ agarose bead (GoldBio, H-310-25) column at 1 mL/min ...
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bioRxiv - Synthetic Biology 2022Quote: ... 100 μg/mL carbenicillin (GoldBio C-103), 25 μg/mL chloramphenicol (GoldBio C-105 ...
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bioRxiv - Biochemistry 2022Quote: Chloramphenicol (Goldbio, cat. no. C-105-5)
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bioRxiv - Bioengineering 2023Quote: ... 100 µg/ml carbenicillin (GoldBio, C-103) and 25 µg/ml chloramphenicol (GoldBio ...
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bioRxiv - Biochemistry 2020Quote: ... and His SUMO tags were cleaved with Ulp1 protease overnight at 4°C in Buffer C (25mM Tris-HCl pH 8.5, 150mM KCl, 2mM TCEP (GoldBio), 50mM Imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... and 0.05% CHAPS (GoldBio, Cat# C-080-100). We pooled and concentrated peak fractions to 5-10μM before we aliquoted and flash froze with liquid nitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... after 10 μM coelenterazine 400a (#C-320, Gold Biotechnology) addition ...
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bioRxiv - Bioengineering 2023Quote: Carbenicillin disodium (Gold Biotechnology cat. no. C-103-5)
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bioRxiv - Biochemistry 2023Quote: ... Then 10 µM of coelenterazine 400a (GoldBio, #C-320) was added to the cells and BRET readings were recorded simultaneously at λemission 410 ± 40 nm (RLU ...
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bioRxiv - Biochemistry 2022Quote: ... 10μl of a 20μM coelenterazine 400A (GoldBio; cat. no. C-320) solution diluted in Krebs/HEPES was added 5min before the end of the stimulation period ...
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bioRxiv - Neuroscience 2023Quote: ... then stimulated with the coelenterazine 400A substrate (5 mM) (Gold Biotechnology #C-320-1) and read for 30 min on Mithras2 Plate Reader (Berthold ...
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bioRxiv - Physiology 2022Quote: ... N2 worms were egg-prepped onto solid NGM with 50 μg/ml carbenicillin (GoldBio, C-103-5) and HT115(DE3 ...
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bioRxiv - Biophysics 2024Quote: ... at 18 °C for 16 hours after induction with 0.2 mM isopropyl-β-D-thiogalactoside (Goldbio, I2481C100). The cells were resuspended and disrupted in lysis buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2021Quote: ... Each mouse was intraperitoneally injected with 3-mg D-luciferin (Gold Biotechnology Inc.) in 200-μL PBS before observation ...
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bioRxiv - Biochemistry 2021Quote: ... Next the BRET readings were taken in well-mode by adding coelenterazine 400a (cat. no. C-320, GoldBio) to a final concentration of 10 µM and luminescence emission intensities were simultaneously recorded at 410 ± 40 nm and at 515 ± 15 nm ...
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bioRxiv - Biophysics 2023Quote: ... The temperature was then set to 30 °C and protein expression was induced by adding 0.2 % arabinose (Goldbio). Cells were grown for additional 16 hours ...
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bioRxiv - Developmental Biology 2023Quote: ... plants were incubated with an aqueous solution of 100 µg/ml Carbenicillin (GoldBio, C-103-5, lot # 0129.091814A) for 30 minutes.
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bioRxiv - Molecular Biology 2023Quote: ... spread on selective Luria broth (LB) agar plates containing 50 μg/mL carbenicillin (GoldBio Cat #: C-103-25), and grown in a 37 °C incubator overnight ...
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bioRxiv - Biophysics 2020Quote: ... 37 °C) to an OD600 of 0.6-0.8 and induced with 0.75 mM isopropyl β-D-1-thiogalactopyranoside (Gold Biotechnology) for 12 h ...
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bioRxiv - Biophysics 2022Quote: ... Cultures were grown to Abs600 of 0.3 at 37 °C and expression of the encoded construct was induced with 1.0 μM IPTG (Gold Biotechnology). Expression was allowed to continue at 37 °C for 2 h ...
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bioRxiv - Biophysics 2019Quote: ... Cultures were grown to Abs600nm of 0.3 at 37 °C and expression of the encoded construct was induced with 1.0 μM IPTG (Gold Biotechnology). Expression was allowed to continue at 37 °C for 2 h ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were transferred onto fresh media and treated with 100 µg/ml Carbenicillin (GoldBio, C-103-5, lot # 0129.091814A).
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bioRxiv - Microbiology 2023Quote: ... New England Biolabs] were grown at 37 °C in Luria-Bertani (LB) medium supplemented with antibiotics as appropriate for plasmid selection [50 µg mL-1 kanamycin (GoldBio), 25 µg mL-1 chloramphenicol (GoldBio) ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria grew at 37°C and protein induction occurred at 20°C for overnight upon addition of 0.1mM isopropyl D-1-thigalactopyranoside (IPTG; Goldbio, I2481C) to the bacterial culture at mid-log phase (OD600nm=0.6) ...
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bioRxiv - Biochemistry 2021Quote: ... Transformed cells were grown at 37°C until OD 0.3 then grown at 18°C until induction at OD 0.6 with 1 mM IPTG (Gold Biotechnology, Olivette, MO). Cells were grown for 15 hours then centrifuged after which the pellet was resuspended in 20 mM Sodium Phosphate pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... expression cultures were grown at 37 °C to an optical density (OD600) of 3.0 before induction with 500 μM isopropyl-β-D-1-thiogalactopyranoside (IPTG; Gold Biotechnology) and incubation overnight at 16 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were grown at 37 °C to an OD600 of 0.8 and then induced using 1 mM isopropyl-β-d-1-thiogalactopyranoside (IPTG; GoldBio, 2481C) for 8 h at 30 °C ...
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bioRxiv - Cell Biology 2024Quote: ... and cultured axenically at 21°C in HL5 media (Froquet et al., 2009) supplemented with 300 µg/mL Streptomycin Sulfate (Gold Biotechnology) on 10 cm plates ...
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bioRxiv - Microbiology 2020Quote: ... prepared in PBS and then incubated overnight at 37°C (without carbon dioxide) with freshly prepared staining solution (1mg/ml of X-gal (GoldBio Technology, USA) in 40 mM citric acid/sodium phosphate ...
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bioRxiv - Synthetic Biology 2021Quote: ... and to this 0.85 mL of liquid (70 °C) top agar (2XYT media + 0.6% agar) supplemented with 2% Bluo-Gal (GoldBio, CAS #97753-82-7) was added and mixed by pipetting up and down once ...
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bioRxiv - Molecular Biology 2020Quote: ... and grown at 37 °C in LB Broth Miller (EMD) overnight with the respective antibiotics (100 μg/ml ampicillin (Gold Biotechnology, Inc.)) for pAS06 plasmid and 30 μg/ml chloramphenicol (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2022Quote: Individual 6-day-old seedlings expressing a CCA1::LUC reporter grown under LD cycles at 22°C were arrayed on 1/2x MS + 1% Sucrose plates and sprayed with 5mM luciferin (GoldBio, Olivette, MO) prepared in 0.01% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... were transformed and plated on LB Agar (Fisher BioReagents Cat#: BP1425-500) containing 50 µg/mL carbenicillin (GoldBio Cat#: C-103-5) and incubated overnight at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... the temperature was reduced to 20 °C and protein expression was induced with the addition of IPTG (GoldBio, St. Louis, MO, USA) to a final concentration of 1 mM ...
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bioRxiv - Cancer Biology 2022Quote: ... Each mouse was intraperitoneally injected with 3 mg D-luciferin (Gold Biotechnology Inc., St. Louis, MO, USA) in 200 µl PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... tumor-bearing mice were anesthetized by isoflurane inhalation and d-luciferin (Gold Biotechnology; 150mg/kg mouse weight) was administered by intraperitoneal (i.p. ...
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bioRxiv - Bioengineering 2023Quote: ... The cultures were grown at 37°C at 220 rpm and induced at an OD600 of 1-1.2 using 1 mM isopropylthio-β-galactoside (IPTG) (GoldBio, St. Lois, MO, USA). Cultures for fluorescence imaging were grown C aerobically at 16° for 16h post-induction ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was filtered and then incubated with nutation at 4°C for 1 hr with Ni-NTA agarose (Gold Biotechnology #H-350-100) that was pre-equilibrated with lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... coli cultures were grown at 37 °C on LC medium containing either kanamycin or spectinomycin (50 or 100 μg/ml, respectively; Gold Biotechnology, Inc., St. Louis, MO) and used as the host for recombinant plasmids made via sequence and ligation-independent cloning (SLIC ...
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bioRxiv - Molecular Biology 2021Quote: Purification of the mouse lectin-mIgG2a fusion proteins was performed using Protein A agarose resin (Gold Biotechnology, P-400-5). The protein A beads were pelleted at 150g for 5 min and washed once with 1x binding buffer (0.02 M Sodium Phosphate ...
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bioRxiv - Neuroscience 2023Quote: ... containing ProBlock Gold Extra Strength Protease Inhibitor Cocktail Kit (GoldBio, Cat. #: GB-116-2). The cortical lysate was then aliquoted into a 96 well plate and stored at −80°C.
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bioRxiv - Synthetic Biology 2019Quote: ... using the Illumination™ Firefly & Renilla Luciferase Enhanced Assay Kit (Goldbio®, cat# I-920) per manufacturer instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were lysed and assessed for NF-κB activity with an Illumination Firefly Luciferase Enhanced Assay Kit (Gold Biotechnology). The results were normalized by samples’ protein concentrations.