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Citations for Gold Biotechnology :
401 - 450 of 459 citations for D Iditol 1 13C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Antibiotics used in this study include kanamycin (GoldBio #K-120-5, 50 μg mL-1), and rifampicin (GoldBio #R-120-1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Brain sections were incubated in True Black lipofuscin autofluorescence quencher (Goldbio cat # TBH-250-1).
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bioRxiv - Immunology 2022Quote: ... 4.5 mg E1D1 antibody was incubated with 1 mL Protein A resin (GoldBio, P-400) with rotation at room temperature for 30 minutes and then washed thoroughly with PBS ...
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bioRxiv - Biochemistry 2022Quote: ... and the cell pellet was resuspended in 0.5mg ml-1 luciferin (GoldBio; cat. no. LUCNA) solution prepared in 1X HBSS buffer (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... The PCR product was run out on a 1% agarose (GoldBio Cat#: A-201-1000) gel in Tris-Acetate-EDTA (TE ...
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bioRxiv - Microbiology 2023Quote: ... according to the selectable marker expressed by the lentiviral vector (GoldBio, Cat#: H-270-1). Dox-inducible myrAKT-HA fibroblasts (FIG 4 ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µL of which was plated on selective media with and without 1% arabinose (GoldBio) and grown overnight at 37 °C.
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bioRxiv - Plant Biology 2024Quote: ... Leaf discs were harvested after 3 days and exposed to 1 mM luciferin (Gold Biotechnology) for 10 minutes in the dark ...
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bioRxiv - Biochemistry 2024Quote: ... by incubation for 1.5 hrs at room temperature and arrested with 1 mM AEBSF (GoldBio) and 10 mM EDTA ...
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bioRxiv - Biophysics 2022Quote: ... at which time HnSpns expression was induced by the addition of 1 mM IPTG (Gold Biotechnology). The cultures were incubated overnight (∼15 h ...
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DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein EngineeringbioRxiv - Bioengineering 2023Quote: ... Protein expression is induced with the addition of 50 μL of IPTG (GoldBio 367-93-1) to a 1 mM final concentration ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% bovine serum albumin (BSA, #A-420-1, Gold Biotechnology, St. Louis, MO), for phospho-specific antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Molecular mass standards for gels were 100 bp and 1 kb DNA ladders (GoldBio, St. Louis, MO).
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bioRxiv - Plant Biology 2023Quote: ... The primary transformants were screened on ½ MS plates containing 15 µg ml-1 hygromycin B (Gold Biotechnology). After 2 weeks ...
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bioRxiv - Genetics 2022Quote: ... 500 μl of 4 mg/ml stock of Hygromycin solution (Gold Biotechnology, catalog no. H-270-1) was topically added to the bacterial lawn of injection plates to select for transgenic (F1 ...
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bioRxiv - Molecular Biology 2020Quote: ... the old medium was replaced with 60 µl HEPES-buffered explant medium supplemented with luciferin (1 mM) (GoldBio), 10% FBS and antibiotics ...
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bioRxiv - Pathology 2024Quote: ... DNA extraction buffer containing proteinase K in a 1:100 ratio (20mg/ml, GoldBio, Cat # P-480-100) was added to each sample and left for overnight incubation in a shaking block (600rpm ...
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bioRxiv - Biochemistry 2020Quote: ... washed twice with PBS and once with 50 mM HEPES pH 7.6, and resuspended in lysis buffer (50 mM HEPES pH 7.6, 1% Nonidet-P40, 0.2 mM TCEP (Gold Biotechnology), and Roche cOmplete EDTA-free Protease Inhibitor Cocktail) ...
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bioRxiv - Plant Biology 2021Quote: ... The transformants were screened on 0.5× MS plates supplemented with Hygromycin (35 mg/mL, Gold Biotechnology, H-270-1). Candidates were screened for insertion/deletions (indels ...
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bioRxiv - Plant Biology 2021Quote: Fourteen days old plants grown on half strength MS were sprayed with 1 mM Luciferin (Gold Biotechnology, LUCK-1G) with 0.001% triton X-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were collected by centrifugation at 6,000g for 20 min and resuspended in a final volume of 40mL cell lysis buffer (100 mM Tris-HCl, pH 8.0, 1 M NaCl, 20% glycerol, 5 mM TCEP (GoldBio), and 2 cOmplete™ EDTA-free protease inhibitor cocktail tablets (Sigma)) ...
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bioRxiv - Biophysics 2023Quote: ... and resuspended in buffer containing 50 mM Tris pH 8.0 and 150 mM NaCl in the presence of protease inhibitors (2.5 μg ml-1 leupeptin (L-010-100, GoldBio), 1 μg ml-1 pepstatin A (P-020-100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Blots were blocked for 1 h at RT in 1X TBST + 3% BSA Fraction V (Gold Biotechnology, Olivette, MO) and then incubated in primary antibody diluted in blocking buffer with overnight rocking at 4°C (phosphorylated and total EGFR ...
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bioRxiv - Microbiology 2021Quote: ... Lysate was centrifuged at 18,000xg for 1 hr and the supernatant containing soluble protein was bound to nickel agarose beads (Gold Biotechnology), washed with buffer containing 25mM Tris HCl (pH 7.5) ...
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bioRxiv - Cell Biology 2021Quote: GST pulldowns were performed by loading 1 µg of purified GST or GST-p130 onto 15 µL of glutathione beads (GoldBio) in NETN (20 mM Tris pH8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 5 mL of digestion buffer (1 mL of Dispase [Corning, 354235], 50 μL of DNase [5 mg/mL, GoldBio, D-301] ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.5% Triton with 500 μM each of potassium ferrocyanide and potassium ferricyanide and infiltrated with wash buffer as above containing 1 mM X-Gluc (GoldBio). Samples were incubated at 37°C in the dark until staining became visible and were cleared with ethanol for imaging ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were then incubated in X-gal staining solution (X-gal rinse buffer plus 1 mg/mL X-gal (GoldBio X4281C ...
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bioRxiv - Cell Biology 2023Quote: ... The transformants were selected for 4-5 days in 1 ml of YPD broth with clonNAT (200 µg/mL, GoldBio), resulting in the intermediate MTS-mCherry-GFP1-10-clonNat library ...
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bioRxiv - Microbiology 2023Quote: ... New England Biolabs] were grown at 37 °C in Luria-Bertani (LB) medium supplemented with antibiotics as appropriate for plasmid selection [50 µg mL-1 kanamycin (GoldBio), 25 µg mL-1 chloramphenicol (GoldBio) ...
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bioRxiv - Genetics 2023Quote: ... and depleted of non-transposed cells by resuspending the pelleted cells in 300 mL of fresh SCD media containing 1 mg/mL of 5-fluoroorotic acid (ZymoResearch) and 0.1 mg/mL of nourseothricin (Gold Biotechnology) and shaking overnight at 30°C ...
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bioRxiv - Biophysics 2024Quote: ... Cells were harvested by centrifugation at 5,000xg and were resuspended in 40 mL of lysis buffer (100 mM KPi, pH 7.5) supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) (Gold Biotechnology). Cells were lysed by sonication on ice with continuous stirring ...
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bioRxiv - Biochemistry 2024Quote: NADH oxidation data were recorded in the presence of 2 mM ATP (Gold Biotechnology, Inc. p/n: A-081-1), 2 mM phosphoenolpyruvic acid monopotassium salt (PEP) ...
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bioRxiv - Biochemistry 2020Quote: ... Single colonies were isolated from YPD (1% yeast extract, 2% peptone, 2% glucose) agar plates containing 400 μg/ml hygromycin (Gold Biotechnology), and integration was verified by PCR ...
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bioRxiv - Plant Biology 2020Quote: ... Chemiluminescence was detected using the NightSHADE LB 985 system (Berthold) in the presence of 20 mg mL−1 potassium luciferin (Gold Biotech). The LUC/REN ratio was calculated to infer effectiveness of miR775 targeting.
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bioRxiv - Cell Biology 2021Quote: ... cell extraction was carried out as above with the addition of a phosphatase inhibitor cocktail (Simple Stop 1 #GB-450) purchased from Gold Biotechnology, USA ...
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bioRxiv - Biochemistry 2021Quote: ... Transformed cells were grown at 37°C until OD 0.3 then grown at 18°C until induction at OD 0.6 with 1 mM IPTG (Gold Biotechnology, Olivette, MO). Cells were grown for 15 hours then centrifuged after which the pellet was resuspended in 20 mM Sodium Phosphate pH 8 ...
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bioRxiv - Molecular Biology 2023Quote: ... each plate was sprayed with 1 milliliter of an aqueous solution of 5 mM firefly luciferin (Gold Biotechnology, St Louis, MO) and 0.01% (vol/vol ...
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bioRxiv - Microbiology 2023Quote: ... after completion of virus infection virus containing media was replaced with media containing 5 µg/ml MTT reagent (Thiazolyl blue tetrazolium bromide, cat no.298-93-1, Gold biotechnology). Incubate at 37°C for 3-4 hr and then remove MTT containing media and add 100 µl of DMSO to each well to dissolve the formazan crystals formed ...
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bioRxiv - Plant Biology 2024Quote: ... Selection of the transgenic lines was carried out on 1X LS medium containing 25 µg/mL Hygromycin B (H-270-1, GoldBio, USA). Seeds were collected from the yellow-to-brown siliques of T2 transgenic plants and dry stored for 12 days ...
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bioRxiv - Biophysics 2024Quote: ... at 37 °C until OD 0.3 then grown at 18 °C until induction at OD 0.6 with 1 mM IPTG (Gold Biotechnology, Olivette, MO). Cells were grown for 15 h then centrifuged ...
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bioRxiv - Cancer Biology 2021Quote: ... Approximate tumor size was monitored via bioluminescence imaging (Xenogen IVIS Spectrum Imaging System) 1-2x per week following intraperitoneal injection of luciferin reagent (GOLDBIO, #LUCK-1G) at 150mg/kg ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli S17-1 λ pir was routinely grown in Luria Bertani (LB) broth (LabExpress) supplemented with 100 μg/mL carbenicillin (Gold Biotechnology) for plasmid selection.
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bioRxiv - Systems Biology 2023Quote: ... The NAT-resistance cassettes were transformed into FY5 (MATα) and selected on YPD + NAT (200 µg/mL; Gold Biotechnology #N-500-1) plates ...
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bioRxiv - Plant Biology 2020Quote: ... 200 mM IAA in ETOH was first diluted in sterile water and then added to hydroponically growing rice roots approximately 1 day after germination to create a final concentration of 200 nM (GoldBio, I-110-25) ...
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bioRxiv - Genomics 2021Quote: ... and then stained with the indicated antibody in FACS buffer (PBS with 1% FBS, 10 U/ml DNAse I, GoldBio, St. Louis MO) for 30 minutes on ice with gentle agitation ...
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Structural Model of Bacteriophage P22 Scaffolding Protein in a Procapsid by Magic-Angle Spinning NMRbioRxiv - Biochemistry 2024Quote: ... the cells were grown to an OD600 of 0.5 at 37°C before induction of SP with 1 mM IPTG (Gold Biotechnology, St. Louis, MO). For preparation of 5F-Trp-SP ...
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bioRxiv - Cell Biology 2024Quote: ... on the day of the experiment, fresh NGM-RNAi plates supplemented with 1 mM IPTG (Dot Scientific, #DSI5600-25) and 100 μg/mL carbenicillin (GoldBio, #C-103-25) were seeded with HT115 bacteria carrying a pL4440 plasmid expressing double-stranded RNA (dsRNA ...
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bioRxiv - Biochemistry 2022Quote: ... in the medium with the exception of the 293T-based CAPN5-3×FLAG stable cell line that was selected and maintained with 200 µg/ml hygromycin (Gold Biotechnology, H-270-1).
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bioRxiv - Microbiology 2021Quote: ... Fecal samples were collected regularly over 2-6 weeks and plated onto SAB and SAB with nourseothricin (100 μg/mL, Gold Biotechnology, N-500-1) plates.