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Citations for AmsBio :
1 - 49 of 49 citations for RGPD1 2 3 4 5 8 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and membrane protein peptide (by AMSBIO) were procured ...
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bioRxiv - Developmental Biology 2022Quote: 96-well plates with parallel-aligned 2-4 μm electrospun fibers (AMS.TECL-005-8X, AMSBio) were washed with 70% ethanol followed by coating with PO for at least 1 hour at 37°C and laminin for at least 3 hours at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: Parallel-aligned 2-4 μm electrospun fibers fitted to 12-well plate inserts were placed in 12 well plates (AMSBio, AMS-TECL-006-4x). Prior to use ...
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bioRxiv - Neuroscience 2021Quote: ... were washed once with PBS and incubated for 4 hours with 100µg/ml fluoresceinamine labelled glycosaminoglycans: 4-O-sulfated chondroitin sulphate (AMS.CSR-FACS-A1, AMSBIO), poly-sulphated chondroitin sulphate (AMS.CSR-FACS-P1 ...
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bioRxiv - Synthetic Biology 2021Quote: ... resuspension buffer supplemented with 3 μL 1000 U/mL Zymolase (Amsbio) and incubated for 30 min at 37 °C to digest the cell walls ...
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bioRxiv - Microbiology 2021Quote: ... 5 mL of Zymolyase 20T (120494-1, AMSBIO) at 5 mg/ml in 10 mM Na2HPO4 ...
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bioRxiv - Neuroscience 2023Quote: Chondroitin 4-sulfate antibody (C4S; Ly111; Amsbio, Lake Forest, CA) was used to immunoprecipitate C4S from cell lysates of treated and control cells ...
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bioRxiv - Developmental Biology 2020Quote: ... Macrophages were incubated with 3 ug/mL biotinylated Hyaluronan binding protein (Amsbio) overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Type 2 RGF PathClear (Amsbio 3533-010-02). Organoids were cultured in IntesticultTM Organoid Growth media (06005 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μM synthetic retinoid Ec23 (Amsbio #AMS.SRP002-2), 500 nM Smoothened agonist SAG (Millipore #5666600 ...
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bioRxiv - Biochemistry 2021Quote: ... 1-2 mg/mL Zymolyase (AMSBIO, 120493-1)] for lysis at 30°C for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Type 2 RGF PathClear (Amsbio 3533-010-02). In brief ...
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bioRxiv - Bioengineering 2019Quote: ... before transferring the scaffolds into perfusion plates (Amsbio #AMS.AVP-KIT-5) and connecting this to a bioreactor circuit ...
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bioRxiv - Developmental Biology 2020Quote: ... Single cells were resuspended in 40 μL drops of phenol red-free Cultrex RGF BME Type 2 (BME 2, Amsbio, #3533-005-02), and seeded in CellCarrier-96 Ultra Microplates (PerkinElmer ...
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bioRxiv - Developmental Biology 2022Quote: ... Type 2 RGF PathClear (Amsbio, Abbingdon, UK, 3533-010-02). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... reconstituted in CELLBANKER 2® freezing media (Amsbio LLC, 11891) and kept in liquid nitrogen for future use ...
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bioRxiv - Neuroscience 2021Quote: ... and resuspended in 10µg/ml 4-O-sulfated chondroitin sulphate (AMS.CSR-FACS-A1, Amsbio) diluted in freshly prepared PBS (0.8mM CaCl2 ...
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bioRxiv - Neuroscience 2023Quote: ... 2-µm diameter electrospun microfibers (Amsbio, Mimetix aligned 12-well inserts) that mimic the shape of axons were coated with poly-D-lysine and immunopanned OPCs were seeded onto the microfibers in differentiation media.
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bioRxiv - Pharmacology and Toxicology 2019Quote: Arrays of freshly frozen human colorectal tumor (37) and healthy colon tissues (3) were obtained from Amsbio. A list of the 40 different tissues ...
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bioRxiv - Microbiology 2020Quote: ... then suspended again in 3 mL of spheroplasting buffer containing 100 μg oxalyticase (Zymolyase®-20T, AMSBIO). Mixtures were incubated at 37°C for 1 hr with gentle shaking ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were passaged to mitomycin-C inactivated CF-1 MEFs (3×103 cells/cm2; GSC-6101G, Amsbio) in media consisting of DMEM/F12 with 20% Knockout Serum Replacement (10828010 ...
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bioRxiv - Immunology 2023Quote: ... After the PBMC suspension was resuspended in CELLBANKER (Amsbio CELLBANKER 2, 11914), the collected PBMCs were immediately cryopreserved and stored in a freezing container (Nalgene Mr ...
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bioRxiv - Genetics 2020Quote: ... Each PGC line was in culture for approximately 3 weeks before freezing in aliquots of 50,000 cells resuspended in 125 μl of Stem-Cellbanker (Amsbio) and stored at −150 °C.
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bioRxiv - Biochemistry 2023Quote: ... cells were resuspended in 100 µL of buffer Z (50 mM Tris, pH 7.4 and 1-3 mg/ml of zymolyase 100T (Amsbio)) and shaken at 37°C for 1-2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM 2-mercaptoethanol) and incubated with 50 mg/mL zymolyase 100T (AMSBIO) at 37°C for 30-60 minutes until the mixture became clear ...
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bioRxiv - Genomics 2020Quote: ... cells from overnight cultures were pelleted at 1000g for 3 minutes and resuspended in 50 μl of 300U/ml Zymolyase 100-T (AMSBIO) solution ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were resuspended in ice-cold BME (BME 2 RGF, AMSBIO, 3533-010-02) at a density of 3,000 cells per μl ...
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bioRxiv - Microbiology 2022Quote: ... Heparan sulfate was detected by staining with F58-10E4 antibody (5 μg/ml, AmsBio, UK #370255-S) and anti-mouse IgM-AF488 antibodies (2 μg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... 1.5 μg/mL cholesterol (Avanti, 57-88-5) and 1 μg/mL heparan sulfate (Amsbio, AMS.GAG-HS01) (TICH media) ...
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bioRxiv - Genomics 2021Quote: ... ESC were adapted to 2i media (serum-free N2B27 medium supplemented with MEK inhibitor PD0325901 [0.4 μM, Miltenyi Biotec], GSK3β inhibitor CHIR99021 [3 μM, Amsbio], and LIF) for at least four days in gelatin-coated tissue culture plates79 ...
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bioRxiv - Molecular Biology 2024Quote: ... Human embryonic kidney HEK293T (ATCC, CRL-3216), human breast cancer MDA-MB-231 (ATCC, HTB-26) and SK-BR-3 (AMSBIO, Abingdon, UK) cell lines were cultured under standard conditions in DMEM supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Molecular Biology 2022Quote: ... UIR and UUO whole kidney lysates (n=4-6 per group) with RNA Stat-60 extraction reagent (Amsbio, CS-111) and purified using the GeneJET RNA purification kit (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM β-mercaptoethanol) and spheroplasted with 2 mg/ml Zymolyase 20T (Amsbio LLC, 120491-1) at 23 °C for 45 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cells were grown as organoids embedded in basement membrane extract (Cultrex BME Type 2, AMSbio) using tumor organoid medium composed of Advanced DMEM/F12 (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... 5 pallia each) were recovered and pooled in PBS and then transferred into pre-warmed FACSmax solution (AMS.T200100, Amsbio), in which the pallia were incubated for 4 min at 28.5°C ...
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bioRxiv - Microbiology 2023Quote: ... 75 cm2 flasks were incubated in TS medium containing 2 μg/mL iMatrix-55 (AMSBIO, Abingdon, UK) for 10 minutes at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: ... 2 × 103 cells were then seeded in each well of a 96-well lipidure plate (Cat.#AMS.51011610, Amsbio) and maintained in GK15 media composed by Glasgow’s MEM (GMEM ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant FIBCD1-FReD18 was added to each well in a constant concentration of 100 ng/ml in TBS containing 0.05% Tween and 5mM CaCl2 together with 2-fold dilutions of CS-4S and CS-6S (Amsbio). After incubation overnight at 4°C and washing ...
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bioRxiv - Microbiology 2020Quote: ... overnight at 22 °C followed with two bead purification steps with 2 volumes of MagSi-NGSPREP Plus beads (AMSBIO). A PCR enrichment test was performed in order to determine the number of PCR cycles that are nesessery for each pool followed by a bead purification step and a QC with DNA HS kit (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... the reactions were neutralized with Acetic acid and were purified with 2 volumes of MagSi-NGSPREP Plus beads (AMSBIO). A second 3Tr3 adaptor were ligated to the cDNA using T4 RNA Ligase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Similar results were also obtained using a commercially available pseudotyped SARS-CoV-2 S protein expressing viral particles (Amsbio LLC).
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bioRxiv - Physiology 2022Quote: Monolayers of HCAEC grown in Transwell membranes were stained for heparan sulfate using mouse monoclonal anti-heparan sulfate antibody (10E4 epitope, 1:100 in 2% goat serum, AMSBIO) following previously established protocols.[31] Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... organoids were suspended in 20μl 80:20 ECM:Media droplets with a protein concentration of between 6.4-9.6mg/ml (BME-2, Amsbio 3533-005-02) on pre-warmed 6-well plates using previously published media recipes2,10 ...
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bioRxiv - Cancer Biology 2020Quote: ... All lines were cultured in drops of Cultrex PathClear Reduced Growth Factor Basement Membrane Extract Type 2 (Amsbio, 3533-005-02) in murine small intestinal organoids medium containing advanced DMEM/F12 medium (adDMEM/F12 ...
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bioRxiv - Cancer Biology 2024Quote: ... cell pellet resuspended in 80% growth factor-reduced basement matrix (either Matrigel®, Corning, 356231; or BME-2®, AMSBIO, 3533) and seeded at the concentration of approximately 50,000 cells/mL by depositing at least six 40 μL drops at the bottom of a non-tissue culture treated plate ...
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bioRxiv - Cell Biology 2021Quote: ... was measured by flow cytometry after incubation for 30 minutes at 4°C with an anti-HS primary antibody (Amsbio Cat#: 370255-S; clone F58-10E4) (1:200 dilution in blocking buffer) ...
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bioRxiv - Cell Biology 2022Quote: ... before high-pressure freezing the carriers containing the 3D cell cultures were dipped for one minute either directly in Cellbanker 2 cryo-preserving medium (catalog number 11891, AMSBIO, Cambridge -US) or in 20% Ficoll 70.000 MW (catalog number F2878 ...
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bioRxiv - Cancer Biology 2021Quote: Dissociated prostate cells were resuspended in 80% growth factor-reduced basement matrix (either Matrigel®,Corning, 356231; or BME-2®,AMSBIO, 3533) and seeded at the concentration of approximately 50,000 cells/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... and mixed at a 1:1 ratio with Cultrex® PathClear Reduced Growth Factors Basement Membrane Extract (BME) Type 2 (Amsbio, Abingdon, England). The BME-cell suspension was seeded as 30 μL drops on prewarmed 24-wells suspension culture plates (Greiner Bio-One ...