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Citations for AmsBio :
1 - 50 of 50 citations for Actin Alpha 2 Smooth Muscle ACTA2 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: hESCs were differentiated to skeletal muscle cells by using commercially available skeletal muscle differentiation medium kit (Amsbio). In brief ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were seeded onto a collagen I (Sigma)-coated dish at 5,000 cells /cm2 dish and maintained for 6 days in skeletal muscle induction medium (SKM01, Amsbio). At day 6 ...
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bioRxiv - Developmental Biology 2020Quote: ... were differentiated three times toward skeletal muscle lineage using commercial media designed from Caron’s work (23) (Skeletal Muscle Induction medium SKM01, Myoblast Cell Culture Medium SKM02, Myotube Cell Culture Medium SKM03, AMSbio). This protocol is a 2D directed differentiation that uses 3 consecutive defined media (SKM01 from day 0 to 10 ...
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bioRxiv - Physiology 2022Quote: Monolayers of HCAEC grown in Transwell membranes were stained for heparan sulfate using mouse monoclonal anti-heparan sulfate antibody (10E4 epitope, 1:100 in 2% goat serum, AMSBIO) following previously established protocols.[31] Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... Type 2 RGF PathClear (Amsbio 3533-010-02). Organoids were cultured in IntesticultTM Organoid Growth media (06005 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μM synthetic retinoid Ec23 (Amsbio #AMS.SRP002-2), 500 nM Smoothened agonist SAG (Millipore #5666600 ...
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bioRxiv - Biochemistry 2021Quote: ... 1-2 mg/mL Zymolyase (AMSBIO, 120493-1)] for lysis at 30°C for 15 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Type 2 RGF PathClear (Amsbio 3533-010-02). In brief ...
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bioRxiv - Immunology 2022Quote: ... and membrane antibody (by AMSBIO) were procured ...
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bioRxiv - Developmental Biology 2020Quote: ... Single cells were resuspended in 40 μL drops of phenol red-free Cultrex RGF BME Type 2 (BME 2, Amsbio, #3533-005-02), and seeded in CellCarrier-96 Ultra Microplates (PerkinElmer ...
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bioRxiv - Cancer Biology 2023Quote: ... Heparan Sulfate 10E4 Antibody from Amsbio, CDH1 (3195) ...
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bioRxiv - Developmental Biology 2022Quote: ... Type 2 RGF PathClear (Amsbio, Abbingdon, UK, 3533-010-02). Briefly ...
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bioRxiv - Biophysics 2024Quote: ... reconstituted in CELLBANKER 2® freezing media (Amsbio LLC, 11891) and kept in liquid nitrogen for future use ...
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bioRxiv - Neuroscience 2023Quote: ... 2-µm diameter electrospun microfibers (Amsbio, Mimetix aligned 12-well inserts) that mimic the shape of axons were coated with poly-D-lysine and immunopanned OPCs were seeded onto the microfibers in differentiation media.
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bioRxiv - Cancer Biology 2020Quote: ... HS was stained using an antibody (370255, Amsbio). Quantification of HA expression of cells in well plates was performed by imaging on a Zeiss Axiovert fluorescent microscope with a 20X objective ...
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bioRxiv - Plant Biology 2021Quote: ... Antibodies used were αGFP (210-PS-1GP, Amsbio) and αHA (11867423001 ...
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bioRxiv - Genomics 2023Quote: ... Human Anti-CD27 agonist antibody (100111-1, AMSBio) and Mouse Anti-CD6 agonist antibody (Clone UMCD6 ...
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bioRxiv - Plant Biology 2024Quote: ... and revealed using anti-GFP antibodies (AMSBIO TP401) and after membrane stripping by streptavidin-HRP (Sigma S2438-250UG) ...
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bioRxiv - Immunology 2023Quote: ... After the PBMC suspension was resuspended in CELLBANKER (Amsbio CELLBANKER 2, 11914), the collected PBMCs were immediately cryopreserved and stored in a freezing container (Nalgene Mr ...
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bioRxiv - Developmental Biology 2021Quote: ... Antibodies used were Δ-Heparan Sulfate (AMSBIO, F69-3G10), Chondroitin 6 Sulfate (Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... 50 mM 2-mercaptoethanol) and incubated with 50 mg/mL zymolyase 100T (AMSBIO) at 37°C for 30-60 minutes until the mixture became clear ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were resuspended in ice-cold BME (BME 2 RGF, AMSBIO, 3533-010-02) at a density of 3,000 cells per μl ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the binding of the 10E4 antibody (clone F58-10E4, Amsbio) to sulfated HS can be quantitated using flow cytometry ...
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bioRxiv - Neuroscience 2023Quote: Chondroitin 4-sulfate antibody (C4S; Ly111; Amsbio, Lake Forest, CA) was used to immunoprecipitate C4S from cell lysates of treated and control cells ...
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bioRxiv - Developmental Biology 2022Quote: 96-well plates with parallel-aligned 2-4 μm electrospun fibers (AMS.TECL-005-8X, AMSBio) were washed with 70% ethanol followed by coating with PO for at least 1 hour at 37°C and laminin for at least 3 hours at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... The following antibodies were used: Heparan sulfate (clone 10e4) from Amsbio; HPSE1 (GTX32650 ...
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bioRxiv - Biochemistry 2020Quote: ... 10 mM β-mercaptoethanol) and spheroplasted with 2 mg/ml Zymolyase 20T (Amsbio LLC, 120491-1) at 23 °C for 45 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Tumor cells were grown as organoids embedded in basement membrane extract (Cultrex BME Type 2, AMSbio) using tumor organoid medium composed of Advanced DMEM/F12 (Gibco) ...
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bioRxiv - Microbiology 2023Quote: ... 75 cm2 flasks were incubated in TS medium containing 2 μg/mL iMatrix-55 (AMSBIO, Abingdon, UK) for 10 minutes at 37°C in 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-HS-stub 3G10 antibody mouse (Amsbio Cat# 370260-1; 1:1000 IB), anti-vGluT1 (Homemade YZ6089 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with primary antibodies for HS (10E4, Amsbio 370255, 1:100) and CS (CS-56 ...
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SARS-CoV-2 accessory proteins ORF3a and ORF7a modulate autophagic flux and Ca2+ homeostasis in yeastbioRxiv - Microbiology 2022Quote: ... The following primary antibodies were used in this work: Rabbit anti-GFP (TP401, Amsbio), mouse anti-flag (M2 ...
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bioRxiv - Cell Biology 2019Quote: ... 2 × 103 cells were then seeded in each well of a 96-well lipidure plate (Cat.#AMS.51011610, Amsbio) and maintained in GK15 media composed by Glasgow’s MEM (GMEM ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant FIBCD1-FReD18 was added to each well in a constant concentration of 100 ng/ml in TBS containing 0.05% Tween and 5mM CaCl2 together with 2-fold dilutions of CS-4S and CS-6S (Amsbio). After incubation overnight at 4°C and washing ...
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bioRxiv - Microbiology 2020Quote: ... overnight at 22 °C followed with two bead purification steps with 2 volumes of MagSi-NGSPREP Plus beads (AMSBIO). A PCR enrichment test was performed in order to determine the number of PCR cycles that are nesessery for each pool followed by a bead purification step and a QC with DNA HS kit (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... the reactions were neutralized with Acetic acid and were purified with 2 volumes of MagSi-NGSPREP Plus beads (AMSBIO). A second 3Tr3 adaptor were ligated to the cDNA using T4 RNA Ligase (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Similar results were also obtained using a commercially available pseudotyped SARS-CoV-2 S protein expressing viral particles (Amsbio LLC).
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bioRxiv - Cancer Biology 2021Quote: ... organoids were suspended in 20μl 80:20 ECM:Media droplets with a protein concentration of between 6.4-9.6mg/ml (BME-2, Amsbio 3533-005-02) on pre-warmed 6-well plates using previously published media recipes2,10 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained with biotin-labelled HABP primary antibody (AMSBIO, Abington, UK, AMS.HKD-BC41, 1:125) overnight at 4°C and stained with fluorescein-conjugated streptavidin (Vector Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... All lines were cultured in drops of Cultrex PathClear Reduced Growth Factor Basement Membrane Extract Type 2 (Amsbio, 3533-005-02) in murine small intestinal organoids medium containing advanced DMEM/F12 medium (adDMEM/F12 ...
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bioRxiv - Cancer Biology 2024Quote: ... cell pellet resuspended in 80% growth factor-reduced basement matrix (either Matrigel®, Corning, 356231; or BME-2®, AMSBIO, 3533) and seeded at the concentration of approximately 50,000 cells/mL by depositing at least six 40 μL drops at the bottom of a non-tissue culture treated plate ...
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bioRxiv - Cell Biology 2022Quote: ... before high-pressure freezing the carriers containing the 3D cell cultures were dipped for one minute either directly in Cellbanker 2 cryo-preserving medium (catalog number 11891, AMSBIO, Cambridge -US) or in 20% Ficoll 70.000 MW (catalog number F2878 ...
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bioRxiv - Microbiology 2022Quote: ... Heparan sulfate was detected by staining with F58-10E4 antibody (5 μg/ml, AmsBio, UK #370255-S) and anti-mouse IgM-AF488 antibodies (2 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: Dissociated prostate cells were resuspended in 80% growth factor-reduced basement matrix (either Matrigel®,Corning, 356231; or BME-2®,AMSBIO, 3533) and seeded at the concentration of approximately 50,000 cells/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... and mixed at a 1:1 ratio with Cultrex® PathClear Reduced Growth Factors Basement Membrane Extract (BME) Type 2 (Amsbio, Abingdon, England). The BME-cell suspension was seeded as 30 μL drops on prewarmed 24-wells suspension culture plates (Greiner Bio-One ...
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bioRxiv - Microbiology 2020Quote: ... then heparan sulfate was stained using 1:30 of F58-10E4 as primary antibody (AMSBIO, cat#370255-S) in PBS 3% BSA for 30 to 40 minutes on ice then washed with PBS 1% FBS and incubated with 1:30 anti-mouse Alexa Fluor 594 (Thermo ...
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bioRxiv - Developmental Biology 2022Quote: Parallel-aligned 2-4 μm electrospun fibers fitted to 12-well plate inserts were placed in 12 well plates (AMSBio, AMS-TECL-006-4x). Prior to use ...
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bioRxiv - Microbiology 2022Quote: ... The neoantigen generated by HS removal (ΔHS) was detected with the F69-3G10 antibody (10 μg/ml, AmsBio #370260-S) and anti-mIgG-AF647 antibodies (4 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... PNN “stubs” after ABC digestion were identified by staining with mouse anti-chontroitin sulfate (1B5) antibody (1:20, Amsbio, 270431-CS). Secondary antibodies were AlexaFluor 405 goat anti-rabbit for PV or AlexaFluor 594 goat anti-mouse (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... was measured by flow cytometry after incubation for 30 minutes at 4°C with an anti-HS primary antibody (Amsbio Cat#: 370255-S; clone F58-10E4) (1:200 dilution in blocking buffer) ...