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Citations for Cambridge Isotope Labs :
1 - 41 of 41 citations for Insulin like growth factor binding protein 6 IGFBP 6 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... CAFs seeded were seeded in 6 well plates at 2×105 cells/well and incubated for 72 hours in growth media containing U13C-Glucose (Cambridge Isotope Laboratories).
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bioRxiv - Cell Biology 2022Quote: ... arginine-6/lysine-8 (heavy, Cambridge Isotope Laboratories ...
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bioRxiv - Microbiology 2020Quote: [1-13C1]-Glucose and [6-13C1]-glucose were purchased from Cambridge Isotope Laboratories Inc ...
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bioRxiv - Physiology 2023Quote: ... and [U-13C]-fructose-6-phosphate (M6-F6P) were purchased from Cambridge Isotope Labs (CLM-1553-PK ...
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bioRxiv - Cancer Biology 2019Quote: ... which was supplemented with 25 mM 6-13C labeled D-glucose (Cambridge Isotope Laboratories). The cells were subjected to this treatment for 12.5 h ...
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bioRxiv - Biophysics 2019Quote: ... except that 13C-D-glucose (Cambridge Isotope Laboratories; at 6 g/L of M9 media) was used as well ...
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bioRxiv - Plant Biology 2024Quote: ... (v/v 2:1) with 10 μM 13C(6)-tyrosine (Cambridge Isotope, CLM-1542-0.25) as an internal recovery standard ...
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bioRxiv - Systems Biology 2023Quote: ... [U-13C]glucose-D-6-phosphate disodium salt hydrate (99%) were purchased from Cambridge Isotope Laboratories (Andover ...
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bioRxiv - Molecular Biology 2021Quote: ... a supplement of [13C6] L-arginine (Arg-6) and [2H4] L-lysine (Lys-4) was added (Cambridge Isotope Laboratories). For the heavy isotope containing medium ...
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bioRxiv - Cell Biology 2022Quote: ... The second plate was cultured in medium SILAC media with the same composition as above except Arg0 and Lys0 were replaced by L-Arginine [13C6] HCl (Arg-6) and L-ysine-4,4,5,5-d4 (Lys-4) (Cambridge Isotope Laboratories). The third plate was cultured in heavy SILAC media with the same composition except arginine and lysine were replaced by L-Arginine [13C6,D7,15N4]HCl (Arg-17 ...
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bioRxiv - Neuroscience 2023Quote: ... 5–10 μL of CSF or serum was extracted in 4:6:3 chloroform:methanol:water mixture supplemented with isotopically labeled T3 and T4 (at 100 nM, Cambridge Isotope Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... 7.0 × 105 cells were plated onto 6 well plates for next day pulse-labeling with either 25 mM [1,2-13C] glucose (Cambridge Isotope Laboratories). Isotope labeling experiments were performed for 4 hours and extra plates were included for cell counts ...
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bioRxiv - Biochemistry 2022Quote: ... modified glucose-free DMEM (Hyclone) was adjusted to 25 mM glucose with either 1-13C-glucose or 6-13C-glucose (Cambridge Isotope Laboratories). Cells were reverse transfected as described above ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.798 mM L-(13C6,15N2)-lysine (Lys”8”)] or “medium-heavy” amino acids [0.398 mM L-(13C6)-arginine (Arg”6”) and 0.798 mM L-(D4)-lysine (Lys”4”)] (Cambridge Isotope Laboratories, Tewksbury, USA). For chloramphenicol (CAP ...
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bioRxiv - Synthetic Biology 2021Quote: ... a 80:20 mixture of 6-13C glucose:U-13C6 glucose or a 80:20 mixture of 1-13C glucose:U-13C6 glucose (Cambridge Isotope, MA and Omicron Biochemicals, IN). Cultures used as internal standards for metabolomic measurements were grown in two subsequent M9 minimal mediums cultures containing 100% U-13C6.
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bioRxiv - Biochemistry 2021Quote: ... BioExpress cell growth media (Cambridge Isotope Laboratories Inc., Andover, MA) was added (2×100 mL of 10X U-15N ...
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Domain-specific quantification of prion protein in cerebrospinal fluid by targeted mass spectrometrybioRxiv - Neuroscience 2019Quote: ... coli in 15N cell growth medium (Cambridge Isotope Laboratories CGM-1000-N) induced with 15N auto-induction medium (Millipore 71759-3) ...
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bioRxiv - Biochemistry 2023Quote: ... 10 mL Bioexpress Cell Growth Media (Cambridge Isotope Laboratories, Tewksbury, MA, USA), 5 mL Gibco™ MEM Vitamin Solution (100x) ...
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bioRxiv - Biochemistry 2021Quote: ... 5 mL 15N Bioexpress Cell Growth Medium (Cambridge Isotope Laboratories, Tewksbury, MA, USA), 5 mL of Gibco MEM Vitamin Solution (100x) ...
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bioRxiv - Biochemistry 2019Quote: ... coli in 15N-enriched cell growth medium (Cambridge Isotope Laboratories CGM-1000-N) supplemented with Overnight Express Autoinduction Medium (Millipore-Sigma 71300-4) ...
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bioRxiv - Biophysics 2022Quote: ... D7 glucose and 20 mL BioExpress cell growth media (Cambridge Isotope Laboratories CGM-1000-DN) per liter added ...
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bioRxiv - Microbiology 2021Quote: Growth trials were conducted for each isolate using labeled substrates (> 98% 13C, 99% 15N, Cambridge Isotope Laboratories, Inc.) to track C into 13C labeled microbial biomass as well as respired 13CO2 ...
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bioRxiv - Biochemistry 2020Quote: ... 15N AIM media was composed of 1X BioExpress 15N cell growth media (Cambridge isotope labs CGM-1000-N) in EMD Millipore Overnight Express induction system (Sigma-Aldrich 71300-M ...
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bioRxiv - Microbiology 2021Quote: The ability of Thaumarchaeota to oxidize DON during growth on NH3 was assessed using 15N isotope tracers (98-99% 15N, Cambridge Isotope Laboratories). Axenic cultures were grown on SCM as described above except using 100 µM ammonium chloride and 20 µM pyruvate ...
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bioRxiv - Biochemistry 2023Quote: Uniformly 15N labeled BTK samples were produced as described earlier by growth in modified M9 minimal media containing 15N ammonium chloride (1g/L, Cambridge Isotope Laboratories, Inc.) as the sole source of nitrogen [39] ...
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bioRxiv - Biochemistry 2021Quote: ... protein was expressed in M9 minimal media with 15N-NH4Cl (Cambridge Isotope labs) as the sole nitrogen source instead of 2xYT media ...
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bioRxiv - Microbiology 2023Quote: ... We spiked 13C and 15N labeled algal AAs or protein (Cambridge Isotope Laboratories, Inc., MA) into late-log phase ...
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Conformational dynamics at microsecond timescale in the RNA-binding regions of dsRNA-binding domainsbioRxiv - Biophysics 2019Quote: ... the protein was prepared by growing the cells in the M9 media containing 15NH4Cl (Cambridge Isotope Laboratories) as a sole source of nitrogen.
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bioRxiv - Molecular Biology 2021Quote: ... and proteins were produced in the M9 media supplemented with 2 g/L [15N]NH4Cl (Cambridge Isotope Laboratories) and 3 g/L d-[13C6]glucose (Cambridge Isotope Laboratories) ...
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bioRxiv - Molecular Biology 2020Quote: ... protein was expressed in BL21/DE3 Escherichia coli cells using M9 minimal media supplemented with 15NH4Cl (Cambridge Isotope Laboratories) induced at an OD600 of 0.6 with 0.5 mM IPTG for 16 hours at 18°C ...
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bioRxiv - Biochemistry 2019Quote: ... assay and immediately diluted (1:1) to a final protein concentration of 0.5 mg/mL with either 18O heavy (Cambridge Isotope Laboratories) or 16O light (Fisher ...
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bioRxiv - Biophysics 2023Quote: ... 15N-labeled and 15N-13C labeled (as required) TRBP2-dsRBD2 protein was prepared using 15NH4Cl and 13C-glucose (Cambridge Isotope Laboratories) as a sole source of nitrogen and carbon ...
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bioRxiv - Biophysics 2024Quote: ... to obtain the 15N uniformly single labelled protein or in a media containing 15NH4Cl (0.1% w/v) and 13C-labelled glucose (0.4% w/v) (Cambridge isotope Laboratories, Inc.) for 15N and 13C uniformly double labelled protein ...
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bioRxiv - Biophysics 2019Quote: ... and uniformly 15N- or 15N,13C-labeled proteins were purified from cells grown in M9 minimal media with 15N ammonium chloride and/or 13C D-glucose (Cambridge Isotope Labs) as the sole nitrogen and/or carbon source ...
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bioRxiv - Biochemistry 2021Quote: ... then 10% of bead volume was lysed with 1% Triton buffer for protein extracts and the remaining 90% was extracted in 80% methanol containing heavy labeled amino acid standards (Cambridge Isotope Labs) on a rotator shaker for 10□min at 4□°C.
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bioRxiv - Biochemistry 2023Quote: ... 15N-labeled protein samples were grown in 1L minimal media containing 3 g/L 15N ammonium chloride (Cambridge Isotope Laboratories, Tweksbury, MA) and unlabeled protein samples were grown in 1L super broth media ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... using a pET-46 tobacco etch virus (TEV) protease-cleavable N-terminal hexahistidine tag fusion protein in M9 media supplemented with 15NH4Cl (Cambridge Isotope Labs, Inc.). Nurr1 LBD was eluted against a 500 mM imidazole gradient through a Ni-NTA column ...
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bioRxiv - Biochemistry 2022Quote: ... and sortase A-compatible proteins were either expressed in Terrific Broth (TB) media or M9 media supplemented with 13C-glucose and/or 15NH4Cl (Cambridge Isotope Labs, Inc.) followed by protein induction with 0.5 mM isopropyl ß-D-1-thiogalactopyranoside (Gold Biotechnology ...
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bioRxiv - Biophysics 2024Quote: ... the cells were transferred to fresh LB medium for the overexpression of the unlabeled protein or to M9 minimal medium supplied with 15NH4Cl (Cambridge Isotope Laboratories Inc.) as the only nitrogen source for the labeled protein overexpression ...
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bioRxiv - Biochemistry 2021Quote: ... cells while expression was carried out in LB media for non-labeled protein and M9 minimal media containing 15NH4Cl (Cambridge Isotope Laboratories, Tewksbury, MA) for 15N ...
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bioRxiv - Cell Biology 2022Quote: ... Ultra pure bovine serum albumin (Cell Signalling Technology 9998) was used as a protein source and iPSDM were incubated with media containing 13C-labeled glucose (Cambridge Isotope Laboratories CLM-1396-5) and the described treatments for 1h before continuing with downstream sample processing ...