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Citations for Akoya Biosciences :
1 - 41 of 41 citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 5 ROIs in 34 WSI from the melanoma dataset were spectrally unmixed using inForm® software v6.4.2 (Akoya Biosciences), and analyzed with the HALO® image analysis platform (Indica Labs) ...
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bioRxiv - Neuroscience 2023Quote: ... The probe was tagged to opal 570 dye from Akoya Biosciences (Marlborough ...
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bioRxiv - Neuroscience 2023Quote: ... The Pvalb probe was tagged with Opal 650 reagent (Akoya #OP001005) and the mCherry probe was tagged with Opal 570 reagent (Akoya #OP001003).
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bioRxiv - Neuroscience 2023Quote: ... and the mCherry probe was tagged with Opal 570 reagent (Akoya #OP001003).
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bioRxiv - Neuroscience 2021Quote: ... fos mRNA was then tagged to the fluorophore fluorescein (1:1500; NEL741E001KT, Akoya Biosciences), and v1aR-mRNA was tagged to the fluorophore Cy5 (1:1500 ...
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bioRxiv - Neuroscience 2021Quote: ... and v1aR-mRNA was tagged to the fluorophore Cy5 (1:1500; NEL745E001KT, Akoya Biosciences). Slides were then rinsed in TBS and stained with a fluorescent Nissl (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse (Cat. No. 468281; Advanced Cell Diagnostics) and Opal 690 Dye (Cat. No. FP1497001KT; Akoya BioSciences) were used according to the manufacturer’s instructions for fluorescence in-situ hybridization ...
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bioRxiv - Immunology 2021Quote: ... A mixture of anti-Mouse + anti-Rabbit HRP-conjugated secondary antibodies (Akoya Biosciences) was added for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... The slides were washed and anti-mouse/anti-rabbit HRP polymer (Akoya Biosciences) was added for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Anti-mouse and anti-rabbit Opal Polymer Horseradish Peroxidase (Opal Polymer HRP Ms + Rb, Akoya Biosciences, Cat. ARH1001EA) were then applied as a secondary label for 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... CODEX staining and imaging was performed on pancreatic mouse tumors utilizing this panel following the manufacturer’s protocol (Akoya Bioscience). Raw fluorescent TIFF image files were processed ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding was visualized using secondary anti-mouse/anti-rabbit polymer HRP followed with TSA-Opal reagents (Akoya NEL811001KT). Tissue slides were incubated with DAPI as a counterstain and coverslipped with VectaShield mounting media (Vector Labs) ...
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bioRxiv - Cancer Biology 2023Quote: ... The flow cell (Akoya Biosciences, #240204) was assembled onto the slide as described in the user guide ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with spectral DAPI (Akoya Biosciences, cat #SKU FP1490), for 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... All images were analyzed using inForm Cell Analysis software (Akoya Biosciences). Sixty-three images of encompassing the 26 different control and experimental slides were batched analyzed after using a mouse formulated library consisting of each single TSA fluorophore listed above ...
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bioRxiv - Cancer Biology 2023Quote: ... images were analyzed using inForm® Cell Analysis™ software (Akoya Biosciences).
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bioRxiv - Developmental Biology 2023Quote: A manual cell classification was performed in CODEX MAV 1.5 (Akoya Biosciences). Annotation of the macrophage clusters was done using the same gating strategy as in flow cytometry ...
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bioRxiv - Cancer Biology 2020Quote: ... Spectral unmixing and cell phenotype identification was performed using inForm software (Akoya Biosciences). Digital immune phenotype plots were created by assembling xy data from identified cell phenotypes in individual tile scans into a whole biopsy composite using ggplot ...
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bioRxiv - Immunology 2021Quote: ... Cells were quantified in a blinded manner using the inForm Software (Akoya Biosciences).
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bioRxiv - Immunology 2023Quote: ... Mixed RNAscope (Advanced Cell Diagnostics)/antibody antigen retrieval and staining with Opal (Akoya Biosciences) fluorophores was performed manually following the RNAscope Multiplex Fluorescent v2 Assay combined with the immunofluorescence protocol (322818-TN ...
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Cell Ecosystem and Signaling Pathways of Primary and Metastatic Pediatric Posterior Fossa EpendymomabioRxiv - Cancer Biology 2020Quote: ... The LGR5 probe (311021, Advanced Cell Diagnostics) was detected using Opal 520 (FP1487001KT, Akoya Biosciences) at a 1:1500 dilution ...
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bioRxiv - Cancer Biology 2021Quote: Supervised machine learning algorithms were applied for tissue and cell segmentation (inForm 2.4.1, Akoya Biosciences). Single-cell-level imaging data were exported and further processed and analyzed using R (v3.6.2) ...
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bioRxiv - Developmental Biology 2021Quote: ... with probe for human TINAGL1(Advanced Cell Diagnostics #857221-C2) and OPAL 570 fluorophore (Akoya #FP1488001KT), following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: Cell numbers of myeloid subsets were estimated with object module of InForm Tissue Analysis Software (AKOYA). Myeloid response score was determined as described previously65,108 ...
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bioRxiv - Immunology 2022Quote: ... Cell classification to detect alveolar macrophages and other mononuclear phagocytes was performed in CODEX MAV (Akoya Biosciences), following a similar gating scheme as the one used for flow cytometry.
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bioRxiv - Cancer Biology 2022Quote: ... anti-ER (Cell Marque, 249-R-15-ASR, clone: SP1, 1:70 dilution) with Opal 650 (Akoya Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... The ER was then visualized incubating the cells using fluorescent tyramide (TSA-FITC 1:200, AKOYA Biosciences), 0.003 H202 and 0,05% Tween-20 in 100 mM borate buffer ...
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bioRxiv - Immunology 2021Quote: ... the RNAScope multiplex fluorescent kit V2 (Advanced Cell Diagnostics) was used with the opal fluorophores system (Akoya Bioscience) according to the manufacturer’s instructions and as described previously66 with some modifications ...
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bioRxiv - Microbiology 2023Quote: ... and analyzed for several immune cell populations using the inForm® tissue Finder (Version 2.4.8) and PhenochartTM (Version 1.0.12) software (Akoya Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... The slide was then transferred to the flow cell carrier and imaged on a PhenoCycler-Fusion (Akoya Biosciences, MA) using the following exposure settings ...
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bioRxiv - Cancer Biology 2023Quote: A T cell panel comprising five markers for five channels was generated for imaging with a Vectra (Akoya Biosciences) as previously described41 for validation purposes ...
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bioRxiv - Immunology 2023Quote: ... The vRNA distribution in relation to T cell and B cell zones was conducted with Voronoi spatial analysis (min:3 µm max:30 µm) using CODEX Multiplex Analysis program (Akoya).
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bioRxiv - Neuroscience 2023Quote: ... CasL/NEDD9 (ACD, 446311), and Sin/EFS (ACD, 806951) probes generated by Advanced Cell Diagnostics were uniquely amplified with Opal dyes (Akoya Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Spectral unmixing and further tissue /cell segmentation as well as phenotyping were performed using the inForm Advanced Image Analysis software (inForm v2.4.10, Akoya Biosciences®). Multiplex data analyses ...
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bioRxiv - Neuroscience 2021Quote: In situ hybridization was performed with the RNAscope® Multiplex Fluorescent Assay v2 from Advanced Cell Diagnostics (ACD) combined with Tyramide Signal Amplification technology (TSA™) and dyes from Akoya Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... based on canonical marker expression values and were confirmed via visual overlay of the cell populations on the immunofluorescent images utilizing the multiplex analysis viewer (MAV, Akoya Biosciences). Voronoi diagrams were created based on the spatial coordinates of the cell types and utilized to mathematically compute adjacent “cell-cell” contacts ...
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bioRxiv - Immunology 2023Quote: ... Slides were acquired on an automated slide scanning brightfield microscope (Vectra) and positive cells were quantified using inForm automated image analysis software (Akoya Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... A training set of 9 representative images was used to train analyses algorithms for tissue and cell segmentation and phenotyping using inForm software (Akoya Biosciences). Representative autofluorescence was measured on an unstained control slide and subtracted from study slides ...
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bioRxiv - Systems Biology 2023Quote: ... RNA in situ detection was performed using the RNAscope Multiplex Fluorescent V2 Assay kit (Advanced Cell Diagnostics) in combination with OPAL dyes 520 and 570 (Akoya Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The green and red dyes were prepared at a concentration of 1:1000 by combining 1000 µl of TSA Buffer (Advanced Cell Diagnostics, 322809) with 1 µl of Opal 520 Reagent (in DMSO, Akoya Biosciences, OP-001001) and Opal 570 Reagent (in DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... Kcnv1 mRNA was detected using the target probe Mm-Kcnv1 (Advanced Cell Diagnostics) and the Opal™ 650 dye (1:1500, Akoya Biosciences, Marlborough, MA, USA)