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Citations for Akoya Biosciences :
1 - 46 of 46 citations for Native Red Fluorescent Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Fluorescent oligonucleotides (Akoya Bioscience) were added to this reporter solution at a final concentration of 1:50 in a total of 250 µl per well ...
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bioRxiv - Developmental Biology 2023Quote: ... Fluorescent oligonucleotides (Akoya Biosciences) were added to this reporter solution at a final concentration of 1:50 in a total of 250 μl per well ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescent slides were counterstained with spectral DAPI (Akoya Biosciences) for 16 minutes before being mounted with ProLong gold antifade (ThermoFischer).
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bioRxiv - Immunology 2024Quote: ... Fluorescent slides were counterstained with spectral DAPI (Akoya Biosciences) for 16 minutes before being mounted with ProLong gold antifade (ThermoFischer ...
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bioRxiv - Neuroscience 2024Quote: ... and 620 fluorescent dyes (1:5000, Akoya Biosystems, FP1487001KT, FP1495001KT). All HRP solutions (C1-C2 ...
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bioRxiv - Neuroscience 2023Quote: ... and TSA plus Cy3 (Red; Akoya Bioscience, Marlborough, MA). Note that the first peroxidase label on the fluorescein probe was completely quenched with 3% H2O2 for 40 mins before the second antibody for the DIG probe was labeled ...
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bioRxiv - Immunology 2023Quote: ... Fluorescent multiplex IHC was achieved using Opal fluorochromes 570 and 690 (Akoya Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... pax7 transcripts were targeted with the fluorescent dyes Opal 520 (#FP1487001KT; Akoya Biosciences). At the end of the in situ hybridization protocol ...
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bioRxiv - Cell Biology 2020Quote: ... Pax7 and Myomixer transcripts were targeted with fluorescent dyes Opal 520 (Akoya Biosciences #FP1487001KT) and Opal 620 (Akoya Biosciences #FP1495001KT ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were developed with Opal fluorescent dye at 1:50 dilution (Akoya Biosciences) in different channels ...
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bioRxiv - Developmental Biology 2023Quote: Fluorescent in situ hybridization was performed using the Tyramide Signal Amplification kit (Akoya Biosciences). A more detailed protocol can be found in the Supplement ...
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bioRxiv - Genetics 2021Quote: ... was used to detect fluorescent signal for Tcfl5 and Opal 690 reagent (Akoya Biosciences, FP1497001KT) for A-Myb ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent oligonucleotide-conjugated reporters were combined with Nuclear Stain and CODEX Assay Reagent (Akoya Biosciences) in light-protected 96-well plates sealed with foil (Akoya Biosciences ...
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bioRxiv - Neuroscience 2022Quote: ... and probes were amplified using TSA fluorescent detection kits as directed by manufacture (Akoya Bioscience). The C1 probe was conjugated to Fluorescein ...
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bioRxiv - Cell Biology 2023Quote: ... Fluorescent detection was performed using an Opal fluorophore tyramide-based signal amplification system (Akoya Biosystems) with the following combinations ...
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bioRxiv - Cell Biology 2023Quote: ... Pax7 and myomixer transcripts were detected using the fluorescent dyes Opal 620 (Akoya Biosciences #FP1495001KT) and Opal 520 (Akoya Biosciences #FP1487001KT) ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were scanned at a Vectra Polaris 1.0 fluorescent slide scanner (Akoya Biosciences, Marlborough, MS, USA) at 20× (0.5 μm/pixel ...
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bioRxiv - Cancer Biology 2022Quote: ... at 85°C prior to the next staining cycles and finally stained with fluorescent DAPI (Akoya) for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... at 85°C prior to the next staining cycles and finally stained with fluorescent DAPI (Akoya) for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: Multiplex immunofluorescence (mIF) analysis was performed with tyramide signal amplification using Opal fluorescent dyes (Akoya Biosciences). Three mIF panels were used in this study (IP1.2 ...
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bioRxiv - Genomics 2020Quote: ... Fluorescent labeling was conducted using Opal 480 (GFAP) and Opal 570 (CD44) dyes (1:150; Akoya Biosciences) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: Brightfield and fluorescent images were acquired using a Mantra 2.0™ Quantitative Pathology Imaging System (Akoya Biosciences). To maximize signal-to-noise ratios ...
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bioRxiv - Cell Biology 2024Quote: ... The ER was then visualized incubating the cells using fluorescent tyramide (TSA-FITC 1:200, AKOYA Biosciences), 0.003 H202 and 0,05% Tween-20 in 100 mM borate buffer ...
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bioRxiv - Immunology 2021Quote: ... the RNAScope multiplex fluorescent kit V2 (Advanced Cell Diagnostics) was used with the opal fluorophores system (Akoya Bioscience) according to the manufacturer’s instructions and as described previously66 with some modifications ...
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bioRxiv - Molecular Biology 2021Quote: ... The binding sites of HRP-conjugated secondary antibodies were detected with Red Opal (1:100, AKOYA Biosciences) and a DAPI counterstain was applied (1:1000) ...
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bioRxiv - Immunology 2022Quote: ... Tissue sections were deparaffined and rehydrated before multiple-color fluorescent staining using Opal 7-Color IHC Kits (Akoya bioscience). Samples were incubated with primary antibody (1:50 ...
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bioRxiv - Genomics 2022Quote: ... an ISH was performed using the RNAScope Multiplex Fluorescent V2 Assay (ACD 323100) with Opal fluorophore reagents (Akoya Biosciences) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2023Quote: ... Sections were counterstained with DAPI and mounted for multispectral fluorescent microscopy using the Vectra Polaris slide scanner (Akoya Biosciences). Quantification was performed using the Indica HALO image analysis platform ...
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bioRxiv - Immunology 2024Quote: Fluorescent and chromogen labeled slides were imaged at 40X using a Vectra PolarisTM Quantitative Pathology Imaging System (Akoya Biosciences). For fluorescently labeled slides ...
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bioRxiv - Neuroscience 2022Quote: ... Bound probes were detected using the RNAscope v2 multiplex fluorescent detection kit and Opal 520 and 690 Dyes (Akoya Biosciences).
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bioRxiv - Developmental Biology 2023Quote: FISH was performed using the RNAscope Multiplex Fluorescent V2 assay (ACDBio, Cat# 323100) using TSA- Cy3 (Akoya Biosciences Cat#NEL744001KT) and TSA-Cy5 (Akoya Biosciences ...
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bioRxiv - Cell Biology 2023Quote: Double fluorescent immunostainings for PARP-1/EpCAM and PARP-1/FRα were performed using Opal Multiplex IHC kit (Akoya Biosciences).
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bioRxiv - Immunology 2023Quote: ... were generated for affiliated brightfield and multiplex fluorescent immunohistochemistry (mfIHC) panels were generated using a multispectral Vectra Polaris Automated Quantitative Pathology Image System (Akoya Biosciences ...
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bioRxiv - Neuroscience 2021Quote: In situ hybridization was performed with the RNAscope® Multiplex Fluorescent Assay v2 from Advanced Cell Diagnostics (ACD) combined with Tyramide Signal Amplification technology (TSA™) and dyes from Akoya Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... The fluorescent Opal 570 dye was added to channel 2 containing SLC17A7-C2 (excitation: 550 nm, emission: 570 nm; Akoya Biosciences). DAPI was added in the final step to spheroids for 30-sec ...
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bioRxiv - Genomics 2020Quote: ... the slides were processed for the standard signal amplification and application of fluorescent dye (Opal dye 520, 570 and 620, AKOYA Biosciences) steps ...
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bioRxiv - Systems Biology 2023Quote: ... RNA in situ detection was performed using the RNAscope Multiplex Fluorescent V2 Assay kit (Advanced Cell Diagnostics) in combination with OPAL dyes 520 and 570 (Akoya Biosciences) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... We used the Six probe 500011-C3 and Jag1 probe 412831-C2 for hybridization and developed each channel with fluorescent dyes Opal Reagent 620 or 690 Reagent Pack (Akoya Biosciences) using RNAscope Multiplex Fluorescent Reagent Kit v2 (Advanced Cell Diagnostics ...
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bioRxiv - Genomics 2023Quote: ... The sections are counterstained with DAPI and mounted for multispectral fluorescent microscopy performed using the PhenoImager HT automated quantitative pathology imaging system (Akoya Biosciences).
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bioRxiv - Immunology 2022Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
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bioRxiv - Immunology 2021Quote: ... protein blocking in Akoya Antibody Diluent/Block (Akoya biosciences, MA) took place for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein blocking was performed using antibody diluent/blocking buffer (Akoya, ARD1001EA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... was stained with 6 protein markers (Figure 4A) and captured with PhenoImager® imaging system (Akoya Biosciences). Over 15,000 cell type annotations from 35 training set slides were used as a training set ...
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bioRxiv - Systems Biology 2023Quote: ... Proximal protein biotinylation occurs with treatment of H2O2 and tyramide-biotin (TSA Biotin System, Akoya Bioscience NEL700A001KT), resulting in tyramide-biotin radicalization and covalent deposition onto proximal proteins ...
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bioRxiv - Cancer Biology 2022Quote: ... tissue slides were exposed to multiple staining cycles each including a 30 minute incubation with a protein block (Akoya), 1 hour incubation with the respective primary antibody ...
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bioRxiv - Cancer Biology 2022Quote: ... tissue slides were exposed to multiple staining cycles each including a 30 minute incubation with a protein block (Akoya), 1 hour incubation with the respective primary antibody ...