Labshake search
Citations for Akoya Biosciences :
1 - 33 of 33 citations for HBSS Solution since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... CODEX fixative solution (Akoya Bioscience) was prepared right before the final fixation step ...
-
bioRxiv - Developmental Biology 2024Quote: ... purification solution (Akoya Biosciences, 7000009) was added ...
-
bioRxiv - Immunology 2021Quote: ... and a fixative solution (AKOYA Biosciences).
-
bioRxiv - Systems Biology 2022Quote: ... DAPI working solution (Akoya biosciences, USA) was applied and the slides were mounted with Prolong Diamond Anti-fade mounting medium (#P36965 ...
-
bioRxiv - Developmental Biology 2024Quote: ... The reduction solution was removed by centrifugation (12,000 x g) and exchanged with the conjugation solution (Akoya Biosciences, 7000009). Each oligonucleotide barcode was rehydrated in 10 µl nuclease-free water (Life Technologies ...
-
bioRxiv - Developmental Biology 2023Quote: ... TSA solutions included TSA-Fluorescein (Akoya Biosciences #NEL741001KT), TSA-CY3 (Akoya Biosciences #SAT704A001EA ...
-
bioRxiv - Cancer Biology 2024Quote: ... Slides were incubated in Spectral DAPI solution (Akoya) for 10 minutes ...
-
bioRxiv - Cancer Biology 2022Quote: ... J and G blocking solutions to CODEX staining buffer (Akoya Bioscience). Antibodies were then added to this blocking buffer in a 1:50 titration to make a total volume of 100 µl ...
-
bioRxiv - Neuroscience 2023Quote: ... the sections were washed and incubated with TSA solution (#NEL744001KT, Akoya Biosciences) overnight at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... The nuclei were stained with spectral DAPI (4,6-diamidino-2-phenylindole) solution (Akoya Biosciences). Finally ...
-
bioRxiv - Physiology 2022Quote: ... to which lyophilized barcodes resuspended in molecular biology grade water and “Conjugation Solution” (Akoya Biosciences) were added and incubated for 2 hours at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... conjugates were purified by four rounds of centrifugation and buffer exchange with Purification Solution (Akoya) through 50 kDa molecular weight cutoff filters (MilliporeSigma) ...
-
bioRxiv - Developmental Biology 2023Quote: ... the same steps were repeated with HRP-C2 solution and OPAL flurophore 570 (Akoya Biosciences). After nuclei staining with DAPI ...
-
bioRxiv - Cancer Biology 2023Quote: ... the PFA solution was made by diluting 16% PFA with CODEX storage buffer (Akoya Biosciences, 232107). The tissue was then washed 3x in 1x PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... followed by a 10-minute incubation with Opal Antibody Dilutent/Block solution (Akoya Bioscience, Marlborough, USA). The first primary antibody was then applied overnight at 4°C ...
-
bioRxiv - Developmental Biology 2022Quote: ... Tyramide Signal Amplification (TSA) solutions were prepared as follows: 1/500 for TSA-Fluorescein (Akoya Biosciences, NEL741001KT), 1/2000 for TSA-CY3 (Akoya Biosciences ...
-
bioRxiv - Neuroscience 2022Quote: ... placed in 1% H2O2 in PBS for 45 minutes and then blocked with TNB solution (0.1 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.5% blocking reagent from Akoya Biosciences) for 1 hour ...
-
bioRxiv - Neuroscience 2022Quote: ... placed in 1% H2O2 in PBS for 1.5 hours and then blocked with TNB solution (0.1 M Tris-HCl, pH 7.5, 0.15 M NaCl, 0.5% blocking reagent from Akoya Biosciences) for 1 hour ...
-
Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... Digoxygenin staining was then developed using the Tyramide PLUS staining solution (TSA PLUS Cy3 reagent, Akoya Biosciences, TS-000202) diluted 1:50 in Amplification buffer for 45 minutes at room temperature ...
-
bioRxiv - Developmental Biology 2023Quote: ... TSA plus cyanine 3 solution (dilute TSA-Cy3 1:250 in 100mM Boric Acid, pH8.5 containing 0.003% H2O2) (Akoya Biosciences) was added to the tissue sections and incubated for 10 min at RT ...
-
bioRxiv - Neuroscience 2021Quote: ... a working Opal dye solution was made fresh using a 1:500 ratio of Opal dye (Akoya Biosciences, Marlborough, MA, USA) to TSA buffer ...
-
bioRxiv - Cancer Biology 2022Quote: ... Reporter stock solution was prepared containing 1:300 Hoechst stain and 1:12 dilution of assay reagent in 1x CODEX buffer (Akoya Bioscience). Fluorescent oligonucleotides (Akoya Bioscience ...
-
bioRxiv - Cancer Biology 2022Quote: ... The tissue was washed and equilibrated before staining for 3 hours at room temperature with the 28-plex CODEX antibody cocktail in a staining buffer containing blocking solution (Akoya Biosciences). After staining ...
-
bioRxiv - Neuroscience 2021Quote: ... channel 1 was established and the slices were incubated in a 1:750 solution of Opal 570 (Akoya Biosciences, catalog # FP1488001KT) diluted in TSA Buffer (ACD) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Filters with 50 kDa molecular weight cut off were first blocked using 500 μl of filter blocking solution (Akoya Biosciences, 7000009). For each conjugation ...
-
bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer and incubated with Cy5-tyramide solution (TSA Plus Cyanine 5; AKOYA Biosciences, NEL745001KT) for 10 min ...
-
bioRxiv - Developmental Biology 2023Quote: ... The samples were then washed with Tris-NaCl-Tween Buffer after incubation with anti-DIG-POD antibodies and incubated with Cy3-tyramide solution (TSA Plus Cyanine 3; AKOYA Biosciences, NEL744001KT) for 10 min ...
-
bioRxiv - Neuroscience 2021Quote: ... The embryos are incubated with the 1:1,000 anti-digoxigenin-POD antibody overnight at 4°C and amplified with Tyramide Amplification working solution and exposed with fluorescein (1:150 dilution of TSA stock with 1x Plus Amplification Diluent-fluorescein) (Akoya Biosciences, Marlborough, MA). All FISH embryos were mounted using DAPI in MOPs buffer and NucBlue (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... for 1 hour at room temperature and amplified with Tyramide Amplification working solution (1:150 dilution of TSA stock with 1x Plus Amplification Diluent-fluorescence) (Akoya Biosciences, Marlborough, MA). Cells were mounted in VectaShield Anti-Fade mounting media with DAPI (Vector Laboratories ...
-
bioRxiv - Cell Biology 2022Quote: ... overnight at 4°C and amplified with Tyramide Amplification working solution (1:150 dilution of TSA stock with 1x Plus Amplification Diluent-fluorescence) (Akoya Biociences, Marlborough, MA). The embryos were washed with MOPS buffer three times then with PBST (1xPBS,0.1% TritonX-100 ...
-
Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... the antibody solution was washed off using PBST and the antibody was developed in dark for 45 minutes without agitation using a tyramide staining solution (TSA PLUS Fluorescein Reagent, Akoya Biosciences, TS-000200) diluted 1:50 in amplification buffer (1X Plus Amplification Diluent ...
-
bioRxiv - Cancer Biology 2024Quote: ... sample slides were washed 3 times in PBS and incubated in final fixative solution (1000uL of PBS + 20uL of Akoya’s final fixation reagent) for 20 minutes at room temperature.
-
bioRxiv - Developmental Biology 2022Quote: ... the samples were incubated in a 1:100 dilution of Cy3-Tyramide Signal Amplification Solution (TSA plus Cyanine 3 Kit, Akoya Biosciences, Marlborough, MA, USA) for 5 minutes at room temperature ...