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Citations for Akoya Biosciences :
1 - 36 of 36 citations for Dl 4 Methoxyestradiol 13 14 15 16 17 18 13C6 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and 16 minute incubation with the Opal reactive fluorophore (Akoya Biosciences). For the last round of staining ...
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bioRxiv - Physiology 2022Quote: 14 of the 23 antibodies used here were conjugated in-house using the protocol outlined by Akoya Biosciences (Table 3)19,20 ...
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bioRxiv - Cancer Biology 2023Quote: ... the PFA solution was made by diluting 16% PFA with CODEX storage buffer (Akoya Biosciences, 232107). The tissue was then washed 3x in 1x PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by 15 minutes at 20% power in AR6 Buffer (Akoya Biosciences, AR600250ML) to affix Opal to target sites and remove primary and secondary antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation in Opal 690 reagent for 15 minutes (Akoya Biosciences, 1:100) in blocking buffer ...
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bioRxiv - Neuroscience 2023Quote: ... v2C2/C3HPR for 15 min and followed by TSA Plus Fluorescein/Cyanine5 (NEL754001KT, Akoya Biosciences) for 30 min at 40°C ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-ER (Cell Marque, 249-R-15-ASR, clone: SP1, 1:70 dilution) with Opal 650 (Akoya Biosciences ...
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bioRxiv - Immunology 2022Quote: ... for 15 minutes and visualized following a 10-minute incubation with Tyramide signal amplification and Opal Fluorophore (Akoya) before being boiled at 100°C to strip antibody-HRP complexes from tissue ...
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bioRxiv - Neuroscience 2023Quote: ... the FITC-labeled probe was visualized using tyramide-FITC Plus (1/50 dilution, 15-minute incubation, Akoya Biosciences). Following washes in TNT ...
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bioRxiv - Cell Biology 2022Quote: ... the Opal 4-color Manual ICH kit (NEL810001KT, AKOYA Biosciences) was used according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... the OPAL 4-Color Manual IHC Kit (Akoya Biosciences NEL810001KT) was used ...
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bioRxiv - Immunology 2022Quote: ... Antigen retrieval was performed by boiling samples to 100°C for 15 minutes in pH 9 Antigen retrieval buffer (Akoya). Tissues were incubated for 45 minutes at room temperature with primary antibodies specific for CD20 (L26 ...
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bioRxiv - Neuroscience 2023Quote: ... ISH was completed by washing slides 3x for 15 minutes in PBS before incubation in Polaris 780 (1:125 in diluent buffer, Akoya) for 30 minutes at 40C ...
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bioRxiv - Immunology 2021Quote: ... we stained slides using Opal 4-Color IHC Kits (AKOYA Biosciences) and observed using the Zeiss LSM 710 or LSM 880 confocal microscope and ZEN microscope software (Carl Zeiss) ...
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bioRxiv - Cancer Biology 2021Quote: ... The PerkinElmer Opal 4-Color Manual IHC Kit (PerkinElmer/Akoya, NEL810001KT) was used for the tyramide signal amplification according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... sections were stained using Opal 4-Color IHC kit (Akoya, NEL810001KT) according to manufacturer’s instructions with the following primary antibodies ...
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bioRxiv - Neuroscience 2023Quote: ... After the described washes and blocking steps the DIG-labeled probes were visualized using tyramide-Cy3 Plus (1/75 dilution, 15-minute incubation, Akoya Biosciences). After washes in TNT ...
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bioRxiv - Cancer Biology 2020Quote: ... included in the Opal 4 color kit (Cat No. NEL810001KT, AKOYA Biosciences) to detect antibody staining ...
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bioRxiv - Cell Biology 2024Quote: ... 15 minutes of Protease III (ACD) at 40°C and 1:750 dilution of OPAL570 or OPAL690 reagents (Akoya Biosciences, Marlborough, MA). Immunohistochemistry for claudin-5 co-localization was performed after RNAScope staining ...
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bioRxiv - Cancer Biology 2021Quote: ... the slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Akoya Biosciences) and coverslipped using ProLong Gold antifade mounting media (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... and IL1B (channel 4; ACD 310361-C4) conjugated to Opal 620 (Akoya FP1495001KT). Expression of IBA1 was then visualized using a secondary antibody against rabbit IgG conjugated to Alexa-Fluor 488 (Life Technologies A21206 ...
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bioRxiv - Neuroscience 2022Quote: ... Each probe was then developed by sequential application of the appropriate HRP reagent (HRP-C1, HRP-C2) for 15 minutes each at 40°C and fluorophore (Akoya Biosciences Opal 690 and Opal 520) for 30 minutes each at 40°C ...
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bioRxiv - Cancer Biology 2020Quote: ... using Opal 4-Color Automation IHC Kit (CK/STING/DAPI) (Cat. No. NEL820001KT, Akoya Biosciences, Marlborough, MA). According to the manufacturer’s instructions ...
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bioRxiv - Pathology 2022Quote: ... visualization of 4-color opal slides was performed by Vectra Polaris Quantitative Pathology Imaging Systems (Akoya, USA).
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bioRxiv - Molecular Biology 2023Quote: ... The tissue sections were then fixed and stored at 4℃° C in Storage Buffer (Akoya Biosciences, MA) until image acquisition ...
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bioRxiv - Immunology 2024Quote: ... Immunofluorescence staining of paraffin embedded tissues were processed using the OPAL 4-Color Manual IHC Kit (Akoya Biosciences, NEL810001KT).
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bioRxiv - Neuroscience 2021Quote: ... Multiplexed immunofluorescent staining was carried out on 4-6µm sections using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table 4) according to Akoya’s CODEX user manual using the CODEX Conjugation Kit (Akoya, 7000009). Briefly ...
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bioRxiv - Immunology 2023Quote: ... and 4-Plex Ancillary Kit (ACD Biotechne, Cat. No. 323120) kits were used in combination with Opal reagents from Akoya Biosciences (Opal 520 – Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... coverslips were washed twice in hydration buffer for a total of 4 minutes followed by fixation in storage buffer (Akoya Bioscience) with 1.6% paraformaldehyde for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... of HNSCC tumors were sectioned to 4-μm thick for the subsequent multiplex immunohistochemistry via the OPAL Polaris system (Akoya Biosciences). After deparaffinization and hydration ...
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bioRxiv - Molecular Biology 2024Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
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bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were washed for 2 days at 4◦C and then incubated for 5 minutes with TSA Cyanine 5 at room temperature (NEL705A001, Akoya, biosciences). All embryos were nuclei stained with DAPI 1:1000 diluted in PBS ...
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bioRxiv - Cancer Biology 2023Quote: Multiplex immunofluorescence staining of AKPT tumours was performed on 4 μm thick FFPE sections using the OPAL protocol (Akoya Biosciences, Marlborough, MA). The Leica BOND RXm autostainer (Leica Microsystems ...
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bioRxiv - Cancer Biology 2022Quote: Multiplex immunofluorescence (MPIF) staining was performed on formalin-fixed paraffin embedded (FFPE) sections of thickness 4-µm using the OPAL protocol (Akoya Biosciences, Marlborough, MA) on the Leica BOND RXm autostainer (Leica Microsystems ...