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1 - 22 of 22 citations for 7 Deacetyl 7 O hemisuccinyl forskolin since 2019

• Multispectral Imaging Differentiates Unique Macrophage Profiles in Patients with Distinct Chronic Liver Diseases

  Omar A. Saldarriaga, et al., bioRxiv - Pathology 2019

  Quote: ... 7-color manual IHC kit (Akoya Biosciences ...
• The circulating immune cell landscape stratifies metastatic burden in breast cancer patients

  S Mangiola, et al., bioRxiv - Cancer Biology 2023

  Quote: Breast tumour sections were stained with antibodies using the Opal 7-7-colour multiplex immunohistochemistry kit (NEL811001KT, Akoya Biosciences). Staining was performed manually per the manufacturer’s protocol ...
• Spatial analysis of the metastatic brain tumor immune and extracellular matrix microenvironment

  Samuel S. Widodo, et al., bioRxiv - Cancer Biology 2022

  Quote: ... with the Opal 7-Color IHC kit (Akoya Biosciences). 4μm thick FFPE tissue sections were baked at 60°C for 1 hour prior to deparaffinization in xylene and rehydration in a series of graded ethanol ...
• Tissue-resident FOLR2⁺ macrophages associate with tumor-infiltrating CD8⁺ T cells and with increased survival of breast cancer patients

  Rodrigo Nalio Ramos, et al., bioRxiv - Immunology 2021

  Quote: ... with Opal™ 7-Color IHC Kits (Akoya Biosciences, NEL821001KT) according to the manufacturer’s instructions (Table S9) ...
• Tissue-resident FOLR2⁺ macrophages associate with tumor-infiltrating CD8⁺ T cells and with increased survival of breast cancer patients

  Rodrigo Nalio Ramos, et al., bioRxiv - Immunology 2021

  Quote: ... with Opal™ 7-Color IHC Kits (Akoya Biosciences, NEL821001KT) according to the manufacturer’s instructions using antibodies shown at Table S9 ...
• Gut microbiota-dependent increase in phenylacetic acid induces endothelial cell senescence during aging

  Seyed Soheil Saeedi Saravi, et al., bioRxiv - Cell Biology 2023

  Quote: ... Using an Opal 7-Color Automation IHC Kit (Akoya Biosciences), Opal singleplex and multiplex for initially-optimized antibodies p-HDAC4Ser632 ...
• AQP1 Differentially Orchestrates Endothelial Cell Senescence

  Khatereh Shabanian, et al., bioRxiv - Molecular Biology 2024

  Quote: ... Using an Opal 7-Color Automation IHC Kit (Akoya Biosciences), Opal singleplex and multiplex for initially-optimized antibodies AQP1 ...
• Human endogenous retrovirus activation contributes to biliary atresia pathogenesis through re-education of resident macrophages

  Jianpeng Sheng, et al., bioRxiv - Pathology 2022

  Quote: ... We adopted Opal PolarisTM 7-color Manual IHC Kit (NEL861001KT, Akoya). Primary antibodies including HERV (LS-C65284 ...
• Multi-modal profiling of the extracellular matrix of human fallopian tubes and serous tubal Intraepithelial carcinomas

  Carine Renner, et al., bioRxiv - Cancer Biology 2021

  Quote: ... Multispectral immunohistochemistry was performed using the Opal 7-Color Manual IHC Kit (Akoya Biosciences/PerkinElmer ...
• Tissue remodeling and cell signaling underpin changes in tumor microenvironment heterogeneity in glioma oncogenesis

  Marija Dinevska, et al., bioRxiv - Cancer Biology 2021

  Quote: Multiplex IHC was performed using an Opal 7-Color IHC Kit (Akoya Biosciences) on a Bond RX automated stainer (Leica Biosystems) ...
• A cellular and spatial map of salivary glands at single cell resolution reveals the functional basis of tertiary lymphoid structure formation in Sjogren’s syndrome

  Saba Nayar, et al., bioRxiv - Immunology 2022

  Quote: ... Staining was performed using the Opal™ 7-Color Automatic IHC Kit (Akoya) according to the manufacturer’s recommendations ...
• Dysregulated coordination of MAPT exon 2 and exon 10 splicing underlies different tau pathologies in PSP and AD

  Kathryn R. Bowles, et al., bioRxiv - Neuroscience 2021

  Quote: Multiplexed immunofluorescent staining was carried out using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
• Multi-scale spatial modeling of immune cell distributions enables survival prediction in primary central nervous system lymphoma

  Margaretha G.M. Roemer, et al., bioRxiv - Systems Biology 2022

  Quote: ... paraffin-embedded whole tissue sections using the Opal 7-color fluorescence immunohistochemistry (IHC) kit (Akoya biosciences, USA), as previously described25 ...
• 4-1BB is a target for immunotherapy in patients with undifferentiated pleomorphic sarcoma

  MJ Melake, et al., bioRxiv - Cancer Biology 2020

  Quote: FFPE tissue sections were stained for multiplex fluorescence-based IHC using the opal 7-colour kit (Akoya Biosciences). Sections were dewaxed and rehydrated ...
• CLEC5A and TLR2 are critical in SARS-CoV-2-induced NET formation and lung inflammation

  Pei-Shan Sung, et al., bioRxiv - Immunology 2022

  Quote: ... Tissue sections were deparaffined and rehydrated before multiple-color fluorescent staining using Opal 7-Color IHC Kits (Akoya bioscience). Samples were incubated with primary antibody (1:50 ...
• The single-cell atlas of the murine reproductive tissues during preterm labor

  Valeria Garcia-Flores, et al., bioRxiv - Immunology 2022

  Quote: OPAL multiplex immunofluorescence staining was performed using the OPAL Multiplex 7-color IHC kit (Cat. no. NEL811001KT; Akoya Biosciences), according to the manufacturer’s instructions ...
• Circadian rhythm disruption alters mammary gland morphology and accelerates “cold” aggressive tumorigenesis through a LILRB4-dependent pathway

  Olajumoke Ogunlusi, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Multiplex immunofluorescence staining was performed with the Akoya Opal™ Polaris 7-color IHC kit fluorophores (Akoya Biosciences [NEL861001KT]). FFPE tissue sections were baked for 30 minutes ...
• 17q21.31 sub-haplotypes underlying H1-associated risk for Parkinson’s disease are associated with LRRC37A/2 expression in astrocytes

  KR Bowles, et al., bioRxiv - Neuroscience 2021

  Quote: ... Multiplexed immunofluorescent staining was carried out on 4-6µm sections using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
• Multiplex Imaging Reveals Novel Subcellular, Microenvironmental, and Racial Patterns of MRTFA/B Activation in Invasive Breast Cancers and Metastases

  Stephanie M. Wilk, et al., bioRxiv - Cancer Biology 2024

  Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
• Integrated Analyses of Multi-omic Data Derived from Paired Primary Lung Cancer and Brain Metastasis Reveals the Metabolic Vulnerability as a Novel Therapeutic Target

  Hao Duan, et al., bioRxiv - Cancer Biology 2024

  Quote: ... The formalin fixed paraffin embedded bullae were sectioned and processed using Opal Polaris™ 7-color Manual IHC Kit (Akoya Biosciences) following the manufacturer’s recommendation ...
• Multilevel human secondary lymphoid immune system compartmentalization revealed by complementary multiplexing and mass spectrometry imaging approaches

  Benjamin L. Oyler, et al., bioRxiv - Immunology 2022

  Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
• Spatial analysis of Hofbauer cell transcriptome, distribution and morphology in placentas exposed to Plasmodium falciparum

  Ricardo Ataide, et al., bioRxiv - Immunology 2023

  Quote: All antibodies used for multiplexed immunofluorescence were validated individually first by chromogenic singleplex immunohistochemistry (IHC) and then by singleplex immunofluorescence using the Opal 7 kit reagents (NEL871001KT, Akoya Biosciences, Waltham, MA, USA). Detailed methods and antibody information are in supplementary data ...