Labshake search
Citations for Akoya Biosciences :
1 - 43 of 43 citations for 7 Amino 4 carboxymethylcoumarin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... Multiplexed immunofluorescent staining was carried out on 4-6µm sections using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
-
bioRxiv - Pathology 2019Quote: ... 7-color manual IHC kit (Akoya Biosciences ...
-
bioRxiv - Cancer Biology 2023Quote: Breast tumour sections were stained with antibodies using the Opal 7-7-colour multiplex immunohistochemistry kit (NEL811001KT, Akoya Biosciences). Staining was performed manually per the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... with the Opal 7-Color IHC kit (Akoya Biosciences). 4μm thick FFPE tissue sections were baked at 60°C for 1 hour prior to deparaffinization in xylene and rehydration in a series of graded ethanol ...
-
bioRxiv - Immunology 2021Quote: ... with Opal™ 7-Color IHC Kits (Akoya Biosciences, NEL821001KT) according to the manufacturer’s instructions (Table S9) ...
-
bioRxiv - Immunology 2021Quote: ... with Opal™ 7-Color IHC Kits (Akoya Biosciences, NEL821001KT) according to the manufacturer’s instructions using antibodies shown at Table S9 ...
-
bioRxiv - Cell Biology 2023Quote: ... Using an Opal 7-Color Automation IHC Kit (Akoya Biosciences), Opal singleplex and multiplex for initially-optimized antibodies p-HDAC4Ser632 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Using an Opal 7-Color Automation IHC Kit (Akoya Biosciences), Opal singleplex and multiplex for initially-optimized antibodies AQP1 ...
-
bioRxiv - Pathology 2022Quote: ... We adopted Opal PolarisTM 7-color Manual IHC Kit (NEL861001KT, Akoya). Primary antibodies including HERV (LS-C65284 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Multispectral immunohistochemistry was performed using the Opal 7-Color Manual IHC Kit (Akoya Biosciences/PerkinElmer ...
-
bioRxiv - Cancer Biology 2021Quote: Multiplex IHC was performed using an Opal 7-Color IHC Kit (Akoya Biosciences) on a Bond RX automated stainer (Leica Biosystems) ...
-
bioRxiv - Immunology 2022Quote: ... Staining was performed using the Opal™ 7-Color Automatic IHC Kit (Akoya) according to the manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2021Quote: Multiplexed immunofluorescent staining was carried out using the Opal Polaris 7 color IHC detection kit (Akoya biosciences) according to manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2022Quote: ... paraffin-embedded whole tissue sections using the Opal 7-color fluorescence immunohistochemistry (IHC) kit (Akoya biosciences, USA), as previously described25 ...
-
bioRxiv - Cancer Biology 2020Quote: FFPE tissue sections were stained for multiplex fluorescence-based IHC using the opal 7-colour kit (Akoya Biosciences). Sections were dewaxed and rehydrated ...
-
bioRxiv - Immunology 2022Quote: ... Tissue sections were deparaffined and rehydrated before multiple-color fluorescent staining using Opal 7-Color IHC Kits (Akoya bioscience). Samples were incubated with primary antibody (1:50 ...
-
bioRxiv - Immunology 2022Quote: OPAL multiplex immunofluorescence staining was performed using the OPAL Multiplex 7-color IHC kit (Cat. no. NEL811001KT; Akoya Biosciences), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... Multiplex immunofluorescence staining was performed with the Akoya Opal™ Polaris 7-color IHC kit fluorophores (Akoya Biosciences [NEL861001KT]). FFPE tissue sections were baked for 30 minutes ...
-
bioRxiv - Cancer Biology 2024Quote: ... Staining was performed on a Leica BOND RX autostainer according to the modified Opal 7-color (v5.2 plus) preset protocol using the Opal 6-Plex Detection kit for Whole Slide Imaging (Akoya Biosciences). Prior to the first staining round ...
-
bioRxiv - Cancer Biology 2024Quote: ... The formalin fixed paraffin embedded bullae were sectioned and processed using Opal Polaris™ 7-color Manual IHC Kit (Akoya Biosciences) following the manufacturer’s recommendation ...
-
bioRxiv - Immunology 2022Quote: ... secondary HRP-labeled antibody and detection with optimized fluorescent Opal tyramide signal amplification (TSA) dye (Opal 7-color Automation IHC kit, from Akoya, Ref. NEL821001KT) and repeated antibody denaturation cycles ...
-
bioRxiv - Cell Biology 2022Quote: ... the Opal 4-color Manual ICH kit (NEL810001KT, AKOYA Biosciences) was used according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2023Quote: ... the OPAL 4-Color Manual IHC Kit (Akoya Biosciences NEL810001KT) was used ...
-
bioRxiv - Immunology 2023Quote: All antibodies used for multiplexed immunofluorescence were validated individually first by chromogenic singleplex immunohistochemistry (IHC) and then by singleplex immunofluorescence using the Opal 7 kit reagents (NEL871001KT, Akoya Biosciences, Waltham, MA, USA). Detailed methods and antibody information are in supplementary data ...
-
bioRxiv - Immunology 2021Quote: ... we stained slides using Opal 4-Color IHC Kits (AKOYA Biosciences) and observed using the Zeiss LSM 710 or LSM 880 confocal microscope and ZEN microscope software (Carl Zeiss) ...
-
bioRxiv - Cancer Biology 2021Quote: ... The PerkinElmer Opal 4-Color Manual IHC Kit (PerkinElmer/Akoya, NEL810001KT) was used for the tyramide signal amplification according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: ... sections were stained using Opal 4-Color IHC kit (Akoya, NEL810001KT) according to manufacturer’s instructions with the following primary antibodies ...
-
bioRxiv - Cancer Biology 2020Quote: ... included in the Opal 4 color kit (Cat No. NEL810001KT, AKOYA Biosciences) to detect antibody staining ...
-
bioRxiv - Cancer Biology 2021Quote: ... the slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Akoya Biosciences) and coverslipped using ProLong Gold antifade mounting media (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2023Quote: ... and IL1B (channel 4; ACD 310361-C4) conjugated to Opal 620 (Akoya FP1495001KT). Expression of IBA1 was then visualized using a secondary antibody against rabbit IgG conjugated to Alexa-Fluor 488 (Life Technologies A21206 ...
-
bioRxiv - Cancer Biology 2020Quote: ... using Opal 4-Color Automation IHC Kit (CK/STING/DAPI) (Cat. No. NEL820001KT, Akoya Biosciences, Marlborough, MA). According to the manufacturer’s instructions ...
-
bioRxiv - Pathology 2022Quote: ... visualization of 4-color opal slides was performed by Vectra Polaris Quantitative Pathology Imaging Systems (Akoya, USA).
-
bioRxiv - Molecular Biology 2023Quote: ... The tissue sections were then fixed and stored at 4℃° C in Storage Buffer (Akoya Biosciences, MA) until image acquisition ...
-
bioRxiv - Immunology 2024Quote: ... Immunofluorescence staining of paraffin embedded tissues were processed using the OPAL 4-Color Manual IHC Kit (Akoya Biosciences, NEL810001KT).
-
bioRxiv - Cancer Biology 2022Quote: ... All other antibodies were custom conjugated to their respective CODEX barcode (Supplemental Table 4) according to Akoya’s CODEX user manual using the CODEX Conjugation Kit (Akoya, 7000009). Briefly ...
-
bioRxiv - Immunology 2023Quote: ... and 4-Plex Ancillary Kit (ACD Biotechne, Cat. No. 323120) kits were used in combination with Opal reagents from Akoya Biosciences (Opal 520 – Cat ...
-
bioRxiv - Cancer Biology 2022Quote: ... coverslips were washed twice in hydration buffer for a total of 4 minutes followed by fixation in storage buffer (Akoya Bioscience) with 1.6% paraformaldehyde for 10 minutes ...
-
bioRxiv - Immunology 2022Quote: ... of HNSCC tumors were sectioned to 4-μm thick for the subsequent multiplex immunohistochemistry via the OPAL Polaris system (Akoya Biosciences). After deparaffinization and hydration ...
-
bioRxiv - Developmental Biology 2024Quote: ... embryos were washed for 2 days at 4◦C and then incubated for 5 minutes with TSA Cyanine 5 at room temperature (NEL705A001, Akoya, biosciences). All embryos were nuclei stained with DAPI 1:1000 diluted in PBS ...
-
bioRxiv - Molecular Biology 2024Quote: ... Experimental sections (4-μm thickness) and positive tissues were stained with specific primary antibodies followed by Opal multiplex immunostaining system (Akoya Biosciences) to generate the stained slides ...
-
bioRxiv - Cancer Biology 2024Quote: mIHC staining was performed according to the manufacturer’s instructions (OpalTM 4-Color Anti-Rabbit Automation IHC; OpalTM Polymer Anti-Rabbit HRP Secondary Antibody Kit; Akoya Biosciences®). The following primary rabbit antibodies were used for staining ...
-
bioRxiv - Cancer Biology 2023Quote: Multiplex immunofluorescence staining of AKPT tumours was performed on 4 μm thick FFPE sections using the OPAL protocol (Akoya Biosciences, Marlborough, MA). The Leica BOND RXm autostainer (Leica Microsystems ...
-
bioRxiv - Cancer Biology 2022Quote: Multiplex immunofluorescence (MPIF) staining was performed on formalin-fixed paraffin embedded (FFPE) sections of thickness 4-µm using the OPAL protocol (Akoya Biosciences, Marlborough, MA) on the Leica BOND RXm autostainer (Leica Microsystems ...