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Citations for Eppendorf :
1 - 31 of 31 citations for Recombinant Human Epidermal Growth Factor Receptor His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
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bioRxiv - Neuroscience 2023Quote: ... The suspension was centrifuged at 1500 rpm for 5 minutes at 4°C and the pellet was resuspended in cold growth factor reduced Matrigel and aliquoted (15 μL per well) in a 48 well plate (Eppendorf, Cat#0030723113). Matrigel was let to polymerize for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... Growth was measured spectrophotometrically at 600 nm using a BioPhotometer (Eppendorf). Spectrometric growth measurements were normalized to bacterial growth without DFO.
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bioRxiv - Synthetic Biology 2024Quote: The growth was conducted in a DASBox mini fermentation system (Eppendorf, Germany). The starting volume of each bioreactor was 150 ml M9 media supplemented with either 30 mM and 10mM D-xylose for the evolved and ancestor samples or only 10mM D-xylose for BW including compact set mutations and/or the deletions used for their insertions (DataS1 file) ...
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bioRxiv - Synthetic Biology 2024Quote: The growth was conducted in a DASBox mini fermentation system (Eppendorf, Germany). The starting volume of each bioreactor was 150 ml M9 media supplemented with 60 mM Formate ...
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bioRxiv - Microbiology 2022Quote: ... Cell growth was monitored as optical density in a spectrophotometer (BioSpectrometer®, Eppendorf) at a wavelength of 600 nm (OD600) ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged Z-B dimers (to 25 nM in 25 µL) in a 1.5-mL LoBind tube (Eppendorf). Then canonical nucleosomes (from native or recombinant source ...
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bioRxiv - Physiology 2019Quote: ... and mitochondrial transcription factor A (TFAM) were quantified by quantitative real-time PCR (Mastercycler® RealPlex2, Eppendorf, Germany), using SYBR Green chemistry (iTaqTM Universal SYBR® Green Supermix ...
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bioRxiv - Microbiology 2019Quote: ... Growth was monitored by measuring optical density at 600 nm (1 cm cuvettes; Eppendorf BioSpectrometer Basic); when OD600 was above 0.5 ...
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bioRxiv - Microbiology 2022Quote: ... Growth was monitored spectrophotometrically by measuring culture optical density at 600 nm (OD600; Eppendorf BioSpectrometer Basic) for up to six weeks ...
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bioRxiv - Microbiology 2023Quote: ... Cell growth was followed by measuring the OD600 in a Bio-Photometer Plus (Eppendorf, Hamburg, Germany).
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bioRxiv - Synthetic Biology 2023Quote: The growth of the various strains was done in a DASBox multi-parallel fermentation system (Eppendorf). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The plasmids encoding wild type Rac1 tagged with GFP (Rac1-wt-GFP) and Rac1-T17N tagged with GFP (Rac1-DN-GFP) were kindly provided by Stefan Linder (University Hospital Hamburg-Eppendorf, Germany).
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bioRxiv - Microbiology 2020Quote: ... Growth was monitored by measuring optical density (OD) at 600 nm (1 cm cuvettes, Eppendorf BioSpectrometer Basic). When OD600 was above 0.5 ...
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bioRxiv - Cell Biology 2023Quote: ... 100 mL of culture growth medium was harvested and centrifuged using the Eppendorf centrifuge 5810 R (Eppendorf, Germany) to remove any additional pellet containing uninfected and iRBCs at 1,800 × g for 15 min ...
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bioRxiv - Immunology 2020Quote: ... The cultures were placed on ice for 10 minutes to slow growth then centrifuged at 3,000 × g for 10 minutes at 4°C (Eppendorf 5810R). The supernatants were discarded and the pellets were resuspended in fresh RPMI 10mM glucose until a McFarland of 5.0 (∼109 CFU/mL ...
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bioRxiv - Molecular Biology 2020Quote: ... one milliliter of NS1 recombinant protein (3 mg/ml in 2x PBS) was placed into a 1.5 ml Eppendorf tube (Eppendorf, Germany); and the tube mixed for 66 hours (55°C ...
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bioRxiv - Neuroscience 2023Quote: ... insoluble PFF were produced by incubation of 300 µM recombinant α-syn in a LoBind reaction tube (Eppendorf GmbH, GE) with one borosilicate glass bead (d = 3.0 mm ...
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bioRxiv - Microbiology 2021Quote: ... 1.5 mL of overnight cultures in LB media in the stationary phase of growth were added to 2mL microcentrifuge tubes (Eppendorf, Canada) and incubated in a 60°C water bath (model FSGPD28 ...
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bioRxiv - Biophysics 2020Quote: ... 200 ml of M9 minimal media were added to 4 mL of pre-inoculum and the cell growth was monitored using Biophotometer D30 (Eppendorf, Hamburg, Germany) until the growth rate was 0.8 (OD at 600 nm) ...
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bioRxiv - Genetics 2022Quote: ... we inoculated yeast strains into 400 µL of liquid SC -lys medium with G418 for overnight growth in 2 mL 96 well plates at 30 °C with 1000 rpm mixing on a MixMate (Eppendorf, Hamburg, Germany). The next day ...
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bioRxiv - Biochemistry 2021Quote: Parasites were cultured in 5% 0+ human erythrocytes (Blood bank, Universitätklinikum Hamburg Eppendorf) in RPMI medium supplemented with 0.5% Albumax at 37°C in an atmosphere of 1% O2 ...
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bioRxiv - Cell Biology 2023Quote: ... human lung primary cells were processed in 1.5 ml DNA LoBind tubes (Eppendorf), washed in PBS via centrifugation at 400g for 5 min at 4 °C and lysed for 3 min on ice before washing via centrifugation at 500g for 5 min at 4 °C ...
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bioRxiv - Microbiology 2020Quote: ... including human and microbial cells (4°C, Eppendorf 5810R centrifuge, 15 min, 3,220 rcf) represented in the “cell pellet” ...
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bioRxiv - Microbiology 2022Quote: ... Trophozoites were harvested from their growth support by incubating the tubes by tapping the glass tubes followed by centrifugation (Eppendorf centrifuge 5810R, rotor A-4-62) according to a previously reported protocol 84.
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bioRxiv - Cell Biology 2020Quote: ... falciparum parasites (strain 3D7) were cultured in human RBCs (O+) (transfusion blood, Universitätsklinikum Hamburg Eppendorf, Hamburg). Cultures were maintained at 37° C in an atmosphere of 1% O2 ...
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bioRxiv - Microbiology 2022Quote: ... falciparum parasites were cultured in human red blood cells (O+ or B+, Universitätsklinikum Eppendorf, Hamburg, Germany) at 5 % haematocrit in an atmosphere of 1% O2 ...
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bioRxiv - Cell Biology 2023Quote: Human erythrocytes (blood group 0+ donated from the blood bank of the University Medical Center Hamburg-Eppendorf) and trophozoites to be examined were washed twice with incomplete TY-I-S-33 medium (200 x g ...
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bioRxiv - Cell Biology 2023Quote: ... falciparum 3D7 [129] were cultured in human red blood cells (O+; University Medical Center Hamburg, Eppendorf (UKE)) ...
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bioRxiv - Molecular Biology 2022Quote: ... falciparum 3D7 (68) were cultured in human red blood cells (O+ or B+, Blood bank, Universitätsklinikum Hamburg-Eppendorf). Cultures were maintained at 37°C in an atmosphere of 1% O2 ...
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bioRxiv - Immunology 2023Quote: ... cells were labeled with 50 nM of biotinylated human ACE2 protein (Acro Biosystems, AC2-H82E6) for 30 minutes at 4°C at 700 RPM on a shaker (Eppendorf, ThermoMixer C). The cells were subsequently washed ...