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Citations for Eppendorf :
501 - 550 of 813 citations for 1 6 Chloropyridazin 3 yl piperidine 4 carboxamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were labeled with 50 nM of biotinylated human ACE2 protein (Acro Biosystems, AC2-H82E6) for 30 minutes at 4°C at 700 RPM on a shaker (Eppendorf, ThermoMixer C). The cells were subsequently washed ...
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bioRxiv - Genomics 2023Quote: ... Cells were pelleted by spinning at 800 g for 10 min at 4 °C in a swinging bucket centrifuge (Eppendorf Centrifuge 5810R). The supernatant was gently removed ...
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bioRxiv - Biophysics 2023Quote: ... This pre-gel solution was kept at 4 °C inside an amber UV-protected 1.5 mL microcentrifuge tube (Eppendorf®, Hauppauge, NY), and degassed for 1 h inside a vacuum chamber to reduce oxygen concentration and prevent early gelation inside the driving syringe while running the experiment ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was centrifuged for 30 min at 15000 rpm at 4°C in the centrifuge Eppendorf 5702RH (Eppendorf SE, Hamburg, Germany). The pellet was resuspended in the 1/5 of the in initial volumes in PBS buffer (ThermoFischer Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Pre-weighted metal-free 15 mL propylene tubes were used to pellet the cells in a tabletop centrifuge at 4 °C (Eppendorf, Hauppauge, NY). Pellets were washed three times with 10 mL of ice-cold PBS ...
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bioRxiv - Cell Biology 2024Quote: ... The sample was placed on ice to incubate for 30 minutes before the demembraned flagella supernatant was removed after centrifugation at 16,000 × g for 10 min at 4°C in a microfuge (Eppendorf, Centrifuge 5415 D). The pellet containing the axoneme was resuspended in 245 μl of Cilia Final Buffer (without trehalose) ...
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bioRxiv - Systems Biology 2024Quote: ... with a mounted tissue section was dried to 37°C for 4 minutes on a thermal incubator (Eppendorf Thermomixer Option C, Germany) followed by in situ fixation in 4% PFA (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... To create extrachromosomal arrays constructs were microinjected into distal gonads of young adults using inverted microscope Leica DMi8 equipped with DIC filters and microinjection system InjectMan® 4 and FemtoJet® 4i (Eppendorf). Microinjection mixtures contained 10 ng/μL of the plasmid of interest ...
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bioRxiv - Developmental Biology 2024Quote: Chromatin was pre-cleared by centrifugation at 14,000×g for 15min at 4°C and the supernatant was moved to a 1.5mL DNA LoBind® tube (Eppendorf: Hamburg, Germany). From each chromatin sample per phenotype per lineage ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was collected after centrifugation at 4 °C for 5 min at 5000 g and dried using a SpeedVaq (Eppendorf, Montesson, France). Then ...
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bioRxiv - Bioengineering 2024Quote: ... 500μl of samples previously diluted by 10 in DPBS were deposited in Amicon® filter before centrifugation at 2000g during 15min at 4°C (Centrifuge 5804 R, Eppendorf®). Then ...
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bioRxiv - Immunology 2024Quote: ... stool suspensions of feces (in H2O) were centrifuged at 16000 rpm and 4°C for 5 min using an Eppendorf 5427R centrifuge (Eppendorf; Hamburg, Germany). 100 µL of supernatant were mixed with 400 µL of ice-cold methanol containing recovery standards for evaluation of the quality of cell harvest and correction for variations ...
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bioRxiv - Immunology 2024Quote: ... Before preparation for LC-MS analysis each sample was centrifuged at 16000 rpm and 4°C for 5 min using an Eppendorf 5427R centrifuge (Eppendorf; Hamburg, Germany). From each supernatant 350 µL were pipetted into two separate LC vials ...
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bioRxiv - Neuroscience 2024Quote: ... The adjacent 3-5 sections of 100 micron tissue sections were collected in a pre-chilled 2mL microcentrifuge tube (Eppendorf Protein LoBind Tube, Cat #22431102) to total a volume of approximately 40 mg for each donor ...
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bioRxiv - Biochemistry 2024Quote: ... clean oocytes collected from the above steps were transferred into 3 μL of master mix at the bottom of 384-well plates (Eppendorf, Protein LoBind, order No. 0030624300) or autosampler vials (Waters ...
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bioRxiv - Microbiology 2021Quote: ... The media was spun down in a centrifuge at 4°C for 10 min at 4400 g (Centrifuge 5920 R, Eppendorf Nordic, Hørsholm, Denmark) and the supernatant collected and stored at -80° for 1H NMR analysis ...
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bioRxiv - Microbiology 2021Quote: ... Development of ∼60 % cytopathic effect (CPE) led to harvesting and clarification of supernatants (3184 x g, 20 min, 4 °C, in an Eppendorf 5810 R centrifuge). Aliquots were then titred by plaque assay (see below) ...
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bioRxiv - Microbiology 2021Quote: The rest of the supernatant cell suspension (~19 ml) was centrifuged in a swing-out rotor (Eppendorf A-4-62 Swing Bucket Rotor) at 3200 × g for 10 min to pellet cells ...
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bioRxiv - Neuroscience 2023Quote: ... blood samples were allowed to clot at room temperature for 30 minutes before undergoing centrifugation at 1500 × g for 10 minutes at 4°C using a refrigerated centrifuge (Eppendorf AG, Hamburg, Germany). The supernatant serum was pipetted into sterile 1.5 mL Eppendorf tubes and stored at -80°C until further analysis.
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bioRxiv - Cell Biology 2024Quote: The cilia suspension was thawed on ice and then centrifuged at 16000 g and 4°C for 10 minutes in a microfuge in a refrigerated room (Eppendorf, Centrifuge 5415 D). The pellet was resuspended in 250 μl of ice-cold Cilia Final Buffer (50 mM HEPES at pH 7.4 ...
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bioRxiv - Physiology 2024Quote: ... Blood was allowed to clot on ice for 20 minutes then was spun down in a cold centrifuge (4°C, Eppendorf microcentrifuge, model 5415R) for 20 minutes at 2000 g ...
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bioRxiv - Biophysics 2021Quote: ... We put 500 mL of log phase density (1×106-1×107 cells/mL) cells into 1 mL disposable cuvettes (UVette, 952010051, Eppendorf AG, Hamburg, Germany). We measured the OD600 every hour for 7 hours in each of two cuvettes for each cell line ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × Denhardt’s solution (Eppendorf, 0032007.155). Hybridization was performed with a double DIG-labeled locked nucleic acid (LNA ...
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bioRxiv - Genomics 2020Quote: The ST slide with the tissue section was warmed to 37°C for 4 minutes on a thermal incubator (Eppendorf Thermomixer Option C, Germany) and in situ fixed and washed as described above ...
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bioRxiv - Microbiology 2020Quote: ... The presumptive cultures were grown in TSBYE broth for 24 h at 30°C and centrifuged at 4°C (Eppendorf, 5810R, Auckland, New Zealand) at 3,220 × g for 10 min ...
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bioRxiv - Biochemistry 2023Quote: ... 12,000 rpm was applied for 30 minutes at 4 °C to obtain the supernatant and then evaporated to obtain a dry metabolite pellet (Eppendorf Concentrator plus, Hamburg, Germany). For the NMR experiments ...
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bioRxiv - Plant Biology 2022Quote: ... with incubation on ice for 1 h followed by centrifugation at 4 °C for 20 min at 20,000 g using an Eppendorf Centrifuge 5430R microcentrifuge (Eppendorf Canada Ltd, Mississauga, ON, Canada), supernatant combined and either used same day or stored at –80 °C.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... a mixture of the antibody constructs with a 10-fold molar excess of DFO*-NCS (5 mg/mL in DMSO) (ABX, Radeberg, Germany) was incubated ON at 4 °C with gentle shaking (Eppendorf ThermoMixer, Merck, Darmstadt, Germany). The DFO*-derivatized constructs were purified by SE-HPLC under the same conditions as described above.
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bioRxiv - Biochemistry 2024Quote: ... Proteins were pelleted by centrifugation at 15.000 x g (10 min, 4°C in cold cabinet, Eppendorf centrifuge #5418, rotor FA-45-18-11). After allowing the pellet to air dry ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with 500μl of D-PBS and centrifuged at 400xg for 10 minutes at room temperature (Eppendorf Centrifuge 5810R, rotor A-4-62). The supernatant was removed and the cell pellet resuspended in 50μl of D-PBS before being stained with CD20-PE (clone A1SB12 ...
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bioRxiv - Neuroscience 2021Quote: ... were incubated with ten-fold molar excess S-XL6 for 4 hours at 37 °C in protein low bind Eppendorf tubes using Eppendorf Thermomixer at 350 rpm (Eppendorf North America, Enfield, CT, USA). After incubation ...
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bioRxiv - Microbiology 2022Quote: ... 1% oxygen (hypoxic; Eppendorf CellXpert incubator), or 0% oxygen (anoxic ...
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bioRxiv - Biochemistry 2020Quote: Microdissected tissue samples were lysed in SDT buffer (4% SDS, 0.1M DTT, 0.1M Tris/HCl, pH 7.6) in a thermomixer (Eppendorf ThermoMixer® C, 30 min, 95°C, 750 rpm). After that ...
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bioRxiv - Microbiology 2022Quote: ... Trophozoites were harvested from their growth support by incubating the tubes by tapping the glass tubes followed by centrifugation (Eppendorf centrifuge 5810R, rotor A-4-62) according to a previously reported protocol 84.
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bioRxiv - Bioengineering 2024Quote: ... Cultures were further incubated overnight (900 RPM, 20 °C, 70 % humidity, 20 h) and harvested by centrifugation (Eppendorf 5810R; 3,220 x g, 4 °C, 15 min). The obtained cell pellet was resuspended with 150 µL buffer (50 mM Bicine ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The sequencing failed for 1 sample and 1 sample was damaged during the travel (Eppendorf tube burst), decreasing our total sample size to 41 ...
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bioRxiv - Plant Biology 2024Quote: ... Duplicate samples of 1 mL were incubated for 1 h at 50 °C in a thermomixer (Eppendorf) with shaking at 600 rpm with 5 µL of CTec3 diluted 10-fold from the commercial product added to each sample ...
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bioRxiv - Cancer Biology 2024Quote: ... 3 times for 5 seconds each to ensure homogenization and centrifuged at 14000 x g for 10 minutes at 4° C (Eppendorf centrifuge 5424 R, FA-45-24-11 rotor). Supernatant was precleared by rotating 20 µl Protein A agarose beads (Cell Signaling #9863 ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein concentration determined by nanodrop (assuming an extinction coefficient of εMolar = 46410 M−1cm−1. The protein solutions were moved into 1 mL aliquots in 1.5 mL Eppendorf tubes ...
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bioRxiv - Microbiology 2022Quote: ... trypsin was inactivated with 1:1 complete media and the cell suspension was collected into a 5mL protein-low bind centrifuge tube (Eppendorf). To remove residual trypsin ...
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bioRxiv - Cancer Biology 2022Quote: ... Pups from these females were injected at P2 with the two plasmids mixed at a ratio of 1:1 using a Femtojet microinjector (Eppendorf) directly into the lateral ventricle ...
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bioRxiv - Neuroscience 2020Quote: ... and RNA concentration was measured in a 1 μl sample diluted 1:200 in nuclease-free water in a spectrophotometer (BioPhotometer, Eppendorf). cDNA was synthesized using 1 μg of input RNA with an iScript™ cDNA Synthesis Kit and MyIQ thermocycler (both Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... The 1 ml of PBS with the resuspended cells was transferred to a 1·5 ml Protein LoBind tube (Eppendorf), centrifuged at 3000 x g for 6 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... 1 min), annealing (65°C, 1 min), and elongation (72°C, 1 min) were performed in a Mastercycler thermal cycler (Eppendorf, Germany). A final extension step was added for 10 min at 72 °C at the end of the 30th amplification cycle ...
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bioRxiv - Neuroscience 2024Quote: ... The forebrain was dissected and weighed before lyophilization at a shelf temperature of –56 °C for 24h under vacuum of 0.2 mBar (Christ LMC-1 BETA 1-16) and extraction with formamide at 57°C for 24h on a shaker at 300 rpm (Eppendorf Thermomixer). Integrated density of tracer fluorescence was determined in triplicates after 1:3 ethanol dilutions ...
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bioRxiv - Cell Biology 2021Quote: ... cells were recovered in 1 mL YPD (1% yeast extract, 2% Bacto peptone, 2% D-glucose) at 30°C in a ThermoMixer C (Eppendorf, Hamburg, Germany) for 3 hours (200 RPM ...
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bioRxiv - Developmental Biology 2021Quote: ... A Femtotip microcapillary (1 μm tip diameter, Eppendorf, Germany) was mounted onto a Femtojet pump and micromanipulator (Eppendorf) ...
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bioRxiv - Cell Biology 2022Quote: ... and agitated (1,300rpm, 30 min, 1°C; Thermomixer Comfort, Eppendorf). Solubilized membrane proteins were obtained in the supernatant from this agitated suspension by centrifugation (21,000g ...
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bioRxiv - Immunology 2020Quote: ... The pH of the eluate (1 mL per Eppendorf tube) was neutralized (125 µL of Tris 1M ...
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bioRxiv - Microbiology 2020Quote: ... The remaining 1 mL of culture was centrifuged (Eppendorf 5417R) at 8000xg and washed twice with 1 mL of LB media ...